Method for detecting crude proteins in castor-oil plant seeds or castor bean meal

A detection method and technology for castor seeds are applied in the field of analysis and determination, and can solve the problems of easy generation of solid precipitation, large amount of catalyst, slow detection speed, etc., and achieve the effects of fast detection speed, short digestion time, and guaranteed accuracy.

Active Publication Date: 2017-11-17
新疆农业科学院生物质能源研究所
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Problems solved by technology

[0007] The purpose of the present invention is to provide a detection method of crude protein in castor seeds or castor cake, which can digest crude protein in castor seeds or castor cake with simple, non-toxic, fast and accurate operation to solve the problem of digestion Long cooking time, slow detection speed, large amount of catalyst used, easy to produce solid precipitation and other problems

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  • Method for detecting crude proteins in castor-oil plant seeds or castor bean meal
  • Method for detecting crude proteins in castor-oil plant seeds or castor bean meal

Examples

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Embodiment 1

[0030] The detection method of crude protein in castor bean seeds, comprises the following steps:

[0031] The castor seeds are dried; the castor seeds are rubbed into slices with a pulverizer or a grinder, as a sample to be tested;

[0032]Weigh 0.5g of the sample to be tested (accurate to 0.0002g), put it into a 250mL Kjeldahl bottle, add 0.2g of copper sulfate, 6g of potassium sulfate, 20mL of concentrated sulfuric acid, 10mL of hydrogen peroxide, and put 2 capsules Glass beads; put a funnel on the mouth of the Kjeldahl nitrogen bottle; place the Kjeldahl bottle on the digestion furnace and heat it with a small fire (starting from about 100°C), try to avoid foam splashing when heating, and when the foam stops, Use a strong fire to gradually increase the heating temperature. The reaction system starts to boil and smoke at about 200°C; continue to smoke at about 250°C; , turning into a black solid; in the process of heating up to 400°C, add 10mL of hydrogen peroxide and cont...

experiment example 1

[0034] The method for digesting and digesting crude protein of the present invention (GB / T6432-1994), a kind of rapid digesting method (CN 103063507 A) for measuring crude protein in feed, the assay method of oilseed crude protein ( The digestion method in GB / T 4489.2-1993) is compared, and then the crude protein content of the sample is measured according to the method of GB / T6432-1994, and the results are shown in Table 1 and Table 2 below:

[0035] Table 1 Digestion time of different castor varieties and different methods

[0036]

[0037] Two castor seeds of different varieties such as Kai feng2 and Kai feng4 imported from Israel were used as test materials to digest crude protein and detect the crude protein content. As can be seen from the experimental results in Table 1, there is a difference between the digestion time of the method of the present invention and the digestion time in other methods. The digestion time difference between the method of the present inven...

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Abstract

The invention relates to a method for detecting crude proteins in castor-oil plant seeds or castor bean meal. The method comprises the following steps: adding copper sulfate, potassium sulfate and concentrated sulfuric acid into a sample to be detected, fully mixing the sample to be detected and the added substances to make the sample to be detected completely wet by the concentrated sulfuric acid; and adding hydrogen peroxide, heating all above materials, carrying out full digestion, and detecting the content of the crude proteins in the above obtained digestion solution. Compared with the prior art, the method disclosed in the invention has the advantages of small relative error, short digestion time, and increase of the digestion speed on the premise of guaranteeing the accuracy. The method allows the digestion time to be short and the detection speed to be fast during the determination of the crude proteins in high-oil plants, and the use amount of a catalyst in the method is very small, so the method is of great realistic significance ecologically and economically.

Description

technical field [0001] The invention belongs to analysis and determination methods, in particular to a method for detecting crude protein in castor seeds or castor cakes. Background technique [0002] Castor bean (Ricinus communis L.) is an annual shrub-like herbaceous plant of the genus Castor in the family Euphorbiaceae (Euphorbiaceae), also known as hemp seed. The plant is tall, branched, and blooms in late summer. The fruit is spherical and splits when ripe. Castor is one of the top ten oil crops. It originated in Egypt, Ethiopia and other countries, and was later introduced to Argentina, Brazil, the United States and other places. At present, castor plantation is mainly distributed in tropical and temperate regions such as India, Brazil, and China (Yao Yuan, Li Fengshan, Chen Yongsheng, etc. Research progress of castor at home and abroad[J]. Journal of Inner Mongolia University for Nationalities, 2009,24(2):172 -175. Ji Zhiqiang, Dong Xue, Gao Liqi, etc. Discussion on...

Claims

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Application Information

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IPC IPC(8): G01N31/16G01N1/28
CPCG01N1/286G01N31/16G01N2001/2866
Inventor 再吐尼古丽·库尔班山其米克吐尔逊·吐尔洪王旭辉王卉古丽尼帕尔·艾合拜尔叶凯涂振东王钊英
Owner 新疆农业科学院生物质能源研究所
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