The invention relates to a kit for diagnosing or detecting leukemia, which contains a lymphocytes separation medium, total RNA extracting solution, reverse transcription buffer solution, M-MLV reverse transcriptase, a reverse transcription primer Oligo(dT)18, an RNA enzyme inhibitor, DEPC water, quantitative PCR buffer solution, Taq DNA polymerase, dNTPs solution, a Wnt5a gene reference standard, an internal reference GAPDH gene reference standard, a primer and a fluorescent probe for detecting a Wnt5a gene and a primer and a fluorescent probe for detecting an internal reference GAPDH gene. The kit for diagnosing or detecting the leukemia has the advantages of simple preparation, convenient detection, rapidness, sensitivity, accurate quantification, high repetitiveness and specificity and suitability for clinical application.