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Tuberculous infection T cell detection kit and detection method thereof

A detection kit and cell technology, applied in the field of immunoassay, can solve the problems of high price, complicated preparation method, and long time consumption, and achieve the effects of convenient and fast production, cost reduction, and simplified separation and purification process

Active Publication Date: 2017-12-01
南通表源生物技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, the existing detection kits for tuberculosis-infected T cells have problems such as complicated preparation methods, long time-consuming, and expensive

Method used

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  • Tuberculous infection T cell detection kit and detection method thereof
  • Tuberculous infection T cell detection kit and detection method thereof

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Embodiment 1

[0032] Human peripheral blood (PBMC) samples were derived from tuberculosis patients and non-tuberculosis populations, and the number was 127 samples to be tested.

[0033] 10ml of blood was collected from each sampled individual with a sodium heparin collection tube, and transported to the laboratory within 6 hours at room temperature.

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Abstract

The invention provides a tuberculous infection T cell detection kit and a detection method thereof. The kit comprises seven polypeptides with amino acid sequences as shown in SEQ ID NO. 1 to SEQ ID NO. 7. The detection method includes the steps: (1) separating a sample to be detected by a Ficoll lymphocyte separation medium, and re-suspending separated cells in a serum-free medium; (2) adding the seven polypeptides with the sequences as shown in SEQ ID NO. 1 to SEQ ID NO. 7 into the re-suspended cells, and then adding gamma interferon antibodies into a co-incubation system to perform antigen antibody reaction; (3) washing the reacted system, adding a chromogenic substrate for chromogenic reaction and counting spots generated by reaction. The kit can be artificially synthesized in a complete sequence manner, is convenient and rapid to produce and high in stability and specificity and has a good commercial application prospect, and cost is greatly reduced.

Description

technical field [0001] The invention belongs to the technical field of immune detection, and in particular relates to a detection kit for T cells infected with tuberculosis and a detection method thereof. Background technique [0002] After the human body is infected with Mycobacterium tuberculosis, Mycobacterium tuberculosis is processed by the antigen-processing cells in the body and presented to the anti-tuberculosis specific T lymphocytes that already exist in the body, but the number is very small, and the T-lymphocytes are stimulated to replicate and expand. At this time, anti-tuberculosis-specific T lymphocytes can be detected in human circulating peripheral blood. However, it is difficult to detect anti-tuberculosis specific T lymphocytes in the blood of people without tuberculosis infection. Therefore, according to the number of anti-tuberculosis specific T lymphocytes in circulating peripheral blood, it can be judged whether they are infected with Mycobacterium tub...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/50
CPCG01N33/505G01N33/6893
Inventor 武尔杰倪佳王志勇王德祥
Owner 南通表源生物技术有限公司
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