Lymphocyte separating medium and preparation method thereof

Abstract

The invention discloses a lymphocyte separating medium and a preparation method thereof, the lymphocyte separating medium is characterized by comprising the following components: glycerin, ethanol, sucrose, meglumine, diatrizoic acid and epichlorohydrin; the sucrose and the diatrizoic acid are added into an ethanol solution to synthesize a water-soluble polysucrose ethanol solution as a molecular sieve separating medium; the method also includes adding meglumine and epichlorohydrin into glycerol, preparing a clear biological solution at room temperature, putting the biological solution into a dialysis paper bag, putting the dialysis paper bag into deionized water, carrying out ion replacement culture for a period of time, and detecting that the dialysis paper bag does not contain redundant foreign ions; then adjusting the pH value of the two solutions to a proper range by using sodium hydroxide and hydrochloric acid; finally, mixing and stirring the two solutions with the pH value adjusted to be stable to be uniform, and obtaining the lymph separation medium. The lymphocyte separation efficiency is high, the separation purity is high, the quality of lymphocytes is improved, high-quality lymphocytes can be obtained, and the lymphocyte separation medium is suitable for clinical application and popularization.

Description

technical field [0001] The invention relates to the technical field of lymphocyte separation, in particular to a lymphocyte separation liquid and a preparation method thereof. Background technique [0002] The separation and purification of pure lymphocytes mainly utilizes the characteristics of single cells adhered to glass, plastic, nylon wool, cotton fiber or dextran gel to remove the mononuclear cells in the mononuclear cell suspension to obtain pure lymphocytes. Lymphocyte population. [0003] At present, lymphocytes are mainly separated by adhesion and adhesion method, adsorption column filtration method, and gradient centrifugation separation method. The existing separation methods have cumbersome operation steps, strong skills, relatively high requirements for the operator's experience, and the separation effect will be greatly improved. Affected, even the lymphocyte layer cannot be separated. In addition, most of the separation liquid has certain toxicity, which af...

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Problems solved by technology

[0005] For the shortcomings of existing technologies, there is currently no solution