The invention relates to a method for separating and purifying placental blood by using a protective fluid and a placenta squeezer and a method for resuscitating the placental blood, and relates to the method for separating and purifying the placental blood by using the protective fluid and the placenta squeezer and the method for resuscitating the placental blood. The purpose of the invention isto solve the problems of difficult separation and extraction of placental blood, low viability of nucleated cells, low number of cells, easy contamination during preparation, and reduced cell qualityduring resuscitation in a conventional method. The placenta squeezer provided by the invention is used, and the protective fluid is added, so that the blood in a placenta is fully squeezed out, and through the efficient separation and purification method, the purposes that the total number of the nucleated cells can reach 15x10<8> or more, the viability is 95% or more, the colony formation is obvious, the phenotype flow detection CD34 reaches 0.3% or more, the pollution rate is controlled within 1%, the viability after resuscitation is 90% or more, the cloning ability is realized, the phenotype flow detection CD34 can reach 0.3% or more, and the sterility test is negative can be ensured. The methods are applied to the field of placental hematopoietic stem cell preparation.