163results about How to "Improve separation and purification efficiency" patented technology

The invention discloses a Process for the purification and separation of trehalose, containing enzymolysis reaction which adds saccharify enzyme in enzyme reaction liquor which is getted from the produced trehalose by enzymic conversion method and inoculating 3-10wt% Saccharomyces cerevisiae in saccharify enzyme enzymolysis liquor, fermenting and culturing in the condition of 25-30deg C and pH 5. 5-7. 5, then getting trehalose pure product after centrifuging, hyperfiltration, ion-exchange, condensation, crystallization and desiccation. Comparing with prevenient activated charcoal column chromatography and organolite process, this invention using two-stage remove mixed sugar of enzymolysis liquor, detecting the getted trehalose pure product by HPLC, its purity is as high as 98. 0%. at the same time, this invention has the advantage of high detaching and purifying efficiency, few lose of trehalose, low cost, simple technology and so on, bringing active impel on predigesting productive technology, improving production and reducing production costs of trehalose's produce by enzyme method.

The invention discloses a method for extracting and purifying anthocyanins from blueberry red leaves. The method is characterized in that two kinds of anthocyanins of which the content is close are contained in a blueberry red leaf anthocyanin extract and account for 93.9 to 99.0 percent of the content of the anthocyanins in the extract. The method comprises the following steps of: selecting fresh blueberry red leaves serving as raw materials, removing leaves which have plant diseases and insect pests and rotted leaves, cleaning by using clean water, drying under vacuum, crushing, sieving, extracting, concentrating under reduced pressure, purifying and separating by macroporous resin, concentrating under the reduced pressure, freeze-drying, saturating, dissolving, growing grains at low temperature, recrystallizing and the like to prepare a natural blueberry red leaf pigment product. By the method, the purity of the recrystallized anthocyanins is over 95 percent; and the anthocyanins are high in purity and simple in composition, and the method is low in production cost and high in popularization.

The invention relates to a preparation method of sterol ester and in particular relates to an enzymatic synthesis method of phytosterol ester / phytostanol ester by utilizing ultrasonic enhancement. The method is characterized by comprising the following steps: 1) preparing a heterogeneous enzyme reaction system: weighing the phytosterol ester / phytostanol ester and fatty acid at a molar ratio of 1:(1.2-2), adding a reaction solvent, and adding enzyme and a molecular sieve; 2) performing enzymatic esterification with ultrasonic assistance: immersing an ultrasonic probe in a reaction solution, opening an ultrasonic wave generator, constantly radiating with ultrasonic wave for 0.5 to 3 hours under mechanical stirring, and continuously reacting for 4 to 10 hours under mechanical stirring after the radiation is completed; and 3) removing the fatty acid with ultrasonic assisted saponification to obtain the product. According to the method, the enzymatic esterification reaction time can be remarkably shortened, the usage of enzyme can be reduced, and the saponification speed of excessive fatty acid can be accelerated, so that the efficiency of separation and purification can be improved, and the obtained phytosterol ester / phytostanol ester product has the characteristics of light color, high purity and safety without contamination.

The invention aims at the field of chemical industry and relates to a separation and purification system of organic synthesis feed liquid and a separation and purification method of the organic synthesis feed liquid. The separation and purification system comprises a crystallization system and a solid-liquid separation system which are sequentially arranged from front to back, wherein the crystallization system comprises a mixed suspension-mixed product-removal (MSMPR) crystallizer and a draft-tube-baffled (DTB) crystallizer which are connected with each other in series; the separation and purification system also comprises a liquid processing system used for processing waste liquid produced by the solid-liquid separation system; the liquid processing system comprises a desalting device and a membrane concentration device which are sequentially arranged from front to back. By virtue of the separation and purification system of the organic synthesis feed liquid and the separation and purification method thereof, the organic synthesis feed liquid is separated and purified by using a continuous crystallization technology, an electrodialysis technology and a membrane separation and concentration technology; the produced waste liquid is fully recycled and used, so that the separation and purification efficiency is improved, the purity and the yield of products are improved and the discharge of the waste liquid is reduced; the separation and purification system of the organic synthesis feed liquid and the separation and purification method thereof are strong in production industrialization and low in costs, and are especially suitable for producing iminodiacetonitrile.

The present invention relates to a preparation method of coenzyme compound. Said invention adopts mature technical processes of temperature difference wall breakage and reduced pressure concentration, and utilizes the steps of ultrafiltering separation, column chromatography to make purification, concentration and precipitation so as to obtain the invented coenzyme compound.

The invention relates to a new technology for preparing poria polysaccharide extract through membrane separation and purification technology. The technology comprises the following steps: (1) grinding and sieving poria powder into crude powder with 20 meshes, and then adding five to ten times of deionized water to obtain feed liquor; (2) filtering the feed liquor obtained in the step (1) through a centrifugal machine; (3) filtering the filtrate obtained in the step (2) through a micro-filtering membrane PP cotton; (4) further filtering the filtrate through the polysulfone hollow fiber type ultrafiltration film with nominal molecular weight cutoff of 10000 dalton; (5) drying the filtrate obtained in the step (4) in a vacuum freeze-drying manner; (6) carrying out anti-oxidization and anti-aging research to the poria polysaccharide by taking a drosophila as an animal model; (7) carrying out hypoglycemic action research by adopting an in-vitro alpha-glucosidase inhibition model through the poria polysaccharide prepared in the step (5). The new technology is simple to operate, and capable of greatly increasing the purity and efficiency of polysaccharide.

A process for preparing the extract from capejasmine fruit by use of macroreticular adsorption resin includes such steps as extracting in hydrophilic solvent to obtain coarse extract, membrane separation for removing impurities, adding water to become suspension, adsorbing by macroreticular resin, water washing for removing polar impurities, eluting with the aqueous solution of hydrophilic solvent, vacuum recovery of solvent, and vacuum concentrating.

The invention discloses a method for separating and preparing anti-tumor components ganoderic acid C1 and ganoderic acid F, which comprises the following steps: (1) primarily enriching and purifying the alcohol extract of lucid ganoderma by use of macroporous resin, and separating a ganoderic acid part with purity of 30-60% from the alcohol extract of lucid ganoderma; (2) separating the separated ganoderic acid part through high-speed counter-current chromatography to obtain ganoderic acid with relatively high purity, wherein a solvent system consists of petroleum ether, ethyl acetate, methanol and water at a volume ratio of 5:(3-7):(1-6):(4-9); the upper phase of the solvent is a stationary phase, and the lower phase is a mobile phase; a sample is dissolved with the lower phase solution and fed; the flow velocity of the stationary phase is 10-20mL / min, the flow velocity of the mobile phase is 1.5-3mL / min, and the rotation speed of the host is 600-1,000rpm; the separated object is collected by an automatic fraction collector, and one fraction is collected from every 4-10mL; (3) analyzing and detecting the components of the liquid collected by each test tube by high performance liquid chromatography, collecting, concentrating and drying according to different components, and separating to obtain ganoderic acid C1 and ganoderic acid F with purity up to 95%. The method has the characteristics of high efficiency, high speed, large separation quantity, high recovery rate, solvent saving and the like.

The invention discloses a device system for purifying by utilizing a gas separation membrane. The device system comprises a mixing device, a pretreatment unit and three gas separation membrane units, wherein two ends of the mixing device are respectively communicated with a raw material gas and the pretreatment unit; a gas passes through the pretreatment unit and enters a gas inlet of the first gas separation membrane unit; an impermeable gas of the first gas separation membrane unit is communicated with a gas inlet of the second separation membrane unit; impermeable gases of the first and second gas separation membrane units pass through a mixed flow tank, and are communicated with a gas inlet of the third separation membrane unit; an impermeable gas outlet of the third separation membrane unit is provided with a backpressure control valve, and is communicated with a gas inlet of the mixing device for cyclic purification; the impermeable gas of the second separation membrane unit is a tail gas; the permeable gas of the third separation membrane unit is a product gas. According to the invention, three membrane units are used for purifying independently so as to completely purify gases, such as helium, hydrogen, carbon dioxide, and the recycling rate is relatively high; the device system is simple and compact in structure, and is applied to industrial production.

The invention relates to a separation and purification method for metallic sodium. The method comprises the following steps that sodium slag is placed in a liquid dispersion medium which has no chemical reaction with the metallic sodium, the temperature of the liquid dispersion medium is increased to be higher than the melting point of the metallic sodium, the temperature of the liquid dispersion medium is decreased to be lower than the boiling point of the metallic sodium after mechanical stirring, filtration is conducted with a filter screen, and metallic sodium particles are obtained after purification. By means of the method, the separation and purification efficiency of the metallic sodium is high, and the recovery rate of the metallic sodium from general sodium slag reaches 90%-98%. As for low-grade sodium slag only containing 20 wt% of the metallic sodium, the recovery rate of the metallic sodium can still reach 80%. By using the method, the troublesome, dangerous and low-value sodium slag in the electrolysis metallic sodium industry can be subjected to secondary development and high value treatment, so that the sodium slag is used to the greatest extent, and waste is turned into wealth.

The invention belongs to the technical field of nano magnetic materials and biology and in particular relates to composite magnetic nanoparticles Fe3O4@Au / MPA / NTA-Ni<2+> as well as preparation and application thereof. BSA (Bull Serum Albumin) surface sulfhydrylation magnetic Fe3O4 nanoparticles and gold nanoparticles are self-assembled under the action of a disulfide bond to form superparamagneticFe3O4@Au nanoparticles; after surfaces of the superparamagnetic Fe3O4@Au nanoparticles are carboxylated by adopting MPA (3-mercaptopropionic acid), coupling with an affinity ligand NTA-Ni<2+> throughan amide bond effect; the prepared Fe3O4@Au / MPA / NTA-Ni<2+> composite magnetic nanoparticles have superparamagnetic performance, the average hydration particle diameter is 100nm to 1000nm, the averageZeta potential is 0 to -25mv and the dispersity is good; when the composite magnetic nanoparticles are applied to protein separation and purification, a process is simple and the separation is rapid;after being circularly used for 6 times, the composite magnetic nanoparticles still have relatively high separation and purification efficiency.

The invention relates to an extraction and separation method for geniposide and gardenia yellow with high yield and high purity. The invention provides a highly-efficient mild aqueous two-phase technology to realize synchronous separation and purification of the geniposide and the gardenia yellow. The method comprises the following steps: preparing gardenia extract by using gardenia fruit powder as a raw material, then subjecting the obtained gardenia extract to extraction and separation through a multistage low-molecular alcohol-salt aqueous two-phase system, carrying out concentration and drying so as to obtain primary geniposide and gardenia yellow crystals, and carrying out recrystallization so as to obtain geniposide crystal with purity of more than 98.5% and gardenia yellow crystal with a color value of 535. The method provided by the invention has the advantages of simple operation, greenness, high efficiency, economy, time conversation and facilitation to enlargement. Meanwhile, the method initiates application of an aqueous two-phase extraction technology in synchronous separation of the gardenia yellow and the geniposide, and improves separation and purification efficiencies of the geniposide and the gardenia yellow.

The invention relates to a method for separating and purifying geniposide by using an isopropanol-salt double-aqueous-phase system. The invention provides an efficient and gentle method for separating and purifying geniposide by using a double-aqueous-phase technique. The method comprises the following steps: producing a waste gardenia yellow liquid from a cape jasmine fruit raw material, performing extraction separation and concentration drying on the waste gardenia yellow liquid by using the isopropanol-salt double-aqueous-phase system, thereby obtaining a primary geniposide crystal product, and further recrystallizing, thereby obtaining geniposide crystal of which the purity is greater than 98.5%. By adopting the method, a natural geniposide product with high purity is separated and purified from a medical resource for both food and medicine. The method is simple and convenient to operate, environmentally friendly, high in efficiency, economical, time-saving and easy to amplify. The method is not only used for widening the double-aqueous-phase system for extracting geniposide and improving the efficiency in separating and purifying geniposide, but also is used for extracting and producing other pigments.

The invention provides a method for purifying lysine, comprising the following steps of: (1) contacting a lysine-containing solution with a cation exchange resin for carrying out ion exchange to ensure that the lysine is adsorbed onto the cation exchange resin, wherein the pH value of the lysine-containing solution is 3.5-6; (2) eluting the cation exchange resin adsorbed with the lysine in the step (1) with an eluent to ensure that the lysine-containing solution adsorbed onto the cation exchange resin by the eluent is replaced so as to obtain a cleaning solution; (3) eluting the lysine adsorbed onto the cation exchange resin in the step (2) with ammonia water to obtain a lysine eluent; and (4) regenerating resin by contacting the cation exchange resin eluted with the ammonia water in the step (3) with a sulphuric acid solution, and reusing the regenerated cation exchange resin in the step (1). According to the method provided by the invention, the exchange adsorption capacity of the lysine and the cation exchange resin can be greatly improved.

The invention relates to a recycling system and a recycling method for waste liquor produced in separation and purification of an organically synthesized feed liquid, and belongs to the field of chemical engineering. The recycling system for the waste liquor comprises a filter device, a desalting device and a membrane concentrating device which are arranged from the front to the rear in sequence, or the filter device is replaced with a filtrate collecting slot, wherein the filter device is used for filtering and collecting the waste liquor; the filtrate collecting slot is used for stewing and collecting the waste liquor; the desalting device is used for desalting the collected waste liquor; the membrane concentrating device is used for concentrating a liquid which is desalted by virtue of the desalting device; and the desalting device is preferably an electrodialysis device. According to the recycling system and the recycling method, a membrane separating and concentrating technology, an electrodialysis technology and the like are comprehensively utilized to separate and purify the organically synthesized feed liquid, and the produced waste liquor is sufficiently recycled, the separating and purifying efficiency and the purity and the yield of products are improved, discharge of the waste liquor is reduced, the production industrialization is strong and the cost is low, and thus, the recycling system and the recycling method are especially suitable for being applied in production of iminodiacetonitrile.

The invention relates to a method for separation of peptide compounds and concretely relates to a method for separation of palmitoyl pentaspeptide-3 by high performance liquid chromatography. The method mainly solves the problem that in the palmitoyl pentapeptide-3 separation, because of small polarity, a lot of an organic solvent is consumed. The method comprises 1, preparing a chromatographic column used in the high performance liquid chromatography, wherein a silica gel column is used as a stationary phase, 2, selecting chromatographic grade acetonitrile as a mobile phase A, and a mobile phase B with a volume ratio of trifluoroacetic acid to methanol or ethanol of 0.001: 1, 3, selecting a mobile phase A gradient range of 5% to 70%, 4, selecting the detection wavelength of 230nm, and 5, selecting an acetate aqueous solution with a mass percent of 0.5% and chromatographic grade acetonitrile as salt transfer mobile phases. The method reduces the retention time of palmitoyl pentaspeptide-3 so as to improve the purification efficiency.

The invention discloses a method for efficiently separating and purifying mammary epithelial cells. The method is suitable for a plurality of mammals. The mammary tissues of adult mammals, which are in the middle and later periods of gestation or in the lactation period, are collected through aseptic operation. According to the provided method, the separation and purification can be achieved in one step, the whole experiment is simple and efficient; the purification, which comprises repeated differential digestion and differential attachment, in the conventional method is eliminated, the purity of mammary epithelial cells obtained by the provided method can reach 98% or more, the obtained mammary epithelial cells can be continuously cultured in-vitro and passed down for 40 generations, and the lactating function of mammary epithelial cells is not affected. The obtained mammary epithelial cells can be applied to the researches on growth of mammary gland, lactating mechanism, mammary bioreactor, transgenic animals, mammary diseases, and the like.

A process for preparing the extract from capejasmine fruit by use of membrane separation technique includes such steps as extracting in hydrophilic solvent to obtain coarse extract, adding water to become suspension, and membrane separation for removing protein, polyose and other macromolecular impurities.

The invention discloses an efficient contrast agent synthesizing method. The method includes the following steps of firstly, preparing an intermediate mixture of a compound shown in the formula (II) and a compound shown in the formula (I) and / or (III); secondly, conducting separating to obtain the compound shown in the formula (II) and the compound shown in the formula (I) and / or (III); thirdly, taking the compound shown in the formula (II) to prepare a contrast agent (iopromide); fourthly, taking the compound shown in the formula (III) to prepare a contrast agent (iobitridol), and / or taking the compound shown in the formula (I) to prepare a contrast agent (iohexol, ioversol, iopentol or iodixanol). In the method, the iodic contrast agent is prepared by synthesizing and separating intermediates in the formula (II) and the formula (I) and / or the formula (III) and using the intermediates as the raw materials, the problem that diacylation byproducts need to be removed in an existing method is effectively solved, all the intermediates are effectively used, efficiency is high, and the actual application prospects are good. The formulas (I), (II) and (III) can be seen in the description.

The present invention adopts single tower, on the basis of general precision rectification process adds flooding operation, its total reflux is implemented at twice, reflux ratio of 4-80, and betweentwo stages of total reflux the light component can be fractionized out from top of tower and the water content is removed. It provides the equipment for implementing the above-mentioned method. It istechnically characterized by adopting high-effective precision rectification filling material and electromagnetic needle pendulum reflux ratio control instrument, height of filling layer is 140-150 cm, its complete condenser is water-splitting complete condenser, and upper portion of filling layer and its middle portion are respectively equipped with returned liquor distributor and oblique cone ring baffle.

A device for separating and cleaning wastewater with rare earth magnet ring, especially a separating and cleaning device for wastewater containing magnetic suspended matter, comprising cradle water tank, rare earth magnrtic ring, supporting framework and cantilevered scum structure. The rare earth magnetic ring comprises ring framework (3), rare earth permanent magnet (4) and ring cover (5), the cantilevered scum structure (10) is connected with hold-down support (12), which connects with cradle (1), the scum sheet (11) is installed on the upper of the rare earth magnetic ring and supports the two sides of the ring cover (5). The device is characterized by little material consumption, light weight, alow cost, sound rigidity, low wastewater current rate in disk, high effecienncy of separating and cleaning and stable property, and the device is the ideal device for treating wastewater containing magnetic suspended matter.

The invention discloses a separation and purification method of an ibrutinib intermediate-(3R)-4-amino-3-(4-phenoxyphenyl)-1-(1-tert-butoxycarbonylpiperidine-3-group)-1-1H-pyrazole [3, 4-d] pyrimidine. The separation and purification method includes: after Mitsunobu reaction for preparing the intermediate stops, adding magnesium chloride into a mixture; cooling after back-flowing; filtering to remove compound precipitate of magnesium chloride and triphenyl phosphine oxide to obtain the intermediate high in purity. Column chromatography is omitted in the process, so that the separation and purification method is high in efficiency and low in cost.

The invention discloses a two-aqueous-phase extraction method of polygonatum sibiricum polysaccharide, and belongs to the technical field of bioengineering. The method solves the problems of low efficiency, high cost and low product purity of existing polygonatum sibiricum polysaccharide separation and purification methods. The method comprises the following steps: crushing polygonatum sibiricum raw materials, degreasing, drying, and performing extraction by using a water extraction method to obtain polygonatum sibiricum polysaccharide extract solution; and extracting and separating the polygonatum sibiricum polysaccharide by adopting a small-molecular organic solvent-soluble salt two-aqueous-phase extraction system, concentrating under reduced pressure, and drying to obtain a purified polygonatum sibiricum polysaccharide product. According to the method, the polygonatum sibiricum polysaccharide is extracted and separated by adopting the small-molecular organic solvent-soluble salt two-aqueous-phase extraction system, so that the separation and purification efficiency is greatly improved, the production cost is reduced, soluble impurities in the polygonatum sibiricum polysaccharideare effectively removed, and the product purity is improved; and the raffinate contains a small amount of polygonatum sibiricum polysaccharide, can be separated and purified again after being recovered, and the method is low in cost and convenient for industrial amplification.

The invention discloses an extraction separation preparation method of lignin monomers in schisandra chinensis, which comprises the following steps: performing CO2 supercritical extraction of schisandra chinensis medicinal material powder, performing separation of crude extract by high-speed countercurrent chromatography, performing gradient elution, wherein the high-speed countercurrent solvent system A comprises n-hexane, ethyl acetate, methanol and water, fully mixing, standing, performing elution with the upper phase A as a stationary phase A and the bottom phase A as a mobile phase A to obtain schisandrol A and schisandrol B, changing the solvent system, increasing the volume ratio of methanol to water, performing elution with the bottom phase B as a mobile phase B to obtain schisantherin B; changing the solvent system, further increasing the volume ratio of methanol to water, performing elution with the bottom phase C as a mobile phase C to obtain schisandrin B. According to the invention, the method combines supercritical fluid extraction with high-speed countercurrent chromatography separation; the product can be used directly for subsequent monomer separation and purification without complicated sample treatment after extraction; 5 lignin monomers can be obtained by only separation once; the method is simple and high-efficient.

The present invention discloses a method for producing cis-decahydronaphthalene through naphthalene hydrogenation. According to the method, a fixed bed reactor is adopted; naphthalene and hydrogen-containing gas enter the reactor; in the presence of a hydrogenation catalyst, a hydrogenation reaction is performed under a hydrogenation reaction condition to obtain the cis-decahydronaphthalene, wherein the hydrogenation catalyst comprises a ZSM-5 molecular sieve, an alkali metal, a VIII group metal, an auxiliary agent and silicon dioxide. With the method of the present invention, the specific catalyst and the suitable reaction condition are adopted, such that the content of the cis-decahydronaphthalene produced through naphthalene hydrogenation can be more than 60 wt%, separation purification efficiency can be significantly improved, and target product production cost can be reduced.

The invention discloses a protein separation and purification device. A feed pot is communicated with an aqueous two-phase system through a first drainage pipe; a distillation device is communicated with an anion exchange resin device through a second drainage pipe. Compared with the traditional technology, the protein separation and purification device provided by the invention is provided with the feed pot, the aqueous two-phase system comprising polymer / salt, and the anion exchange resin device so as to separately perform the protein separation and purification steps, realizes pollution-free step-by-step and segmented treatment from primary separation and purification of the aqueous two-phase system comprising polymer / salt to secondary separation and purification of the anion exchange resin device through feeding of the feed pot, achieves ideal effect, realizes the separation of the protein through adjusting temperature, pH and the like at an early stage, realizes the continuous and automated production of protein separation and purification, and has high separation and purification efficiency.

The invention relates to a preparation method for a glucan bioseparation and purification medium. The preparation method comprises the following steps: adding glucan into water, carrying out heating, stirring and dissolving and then adding an aqueous sodium hydroxide solution so as to obtain an alkaline glucan solution; adding a cross-linking agent into an oil phase and carrying out heating, stirring and dissolving; and rapidly adding the alkaline glucan solution into the oil phase, carrying out heating and violent stirring so as to obtain emulsion droplets with a uniform particle size of 20 to 120 mu m, slowly adding chloropropylene oxide into the emulsion for a reaction, carrying out rapid cooling and then carrying out cleaning with toluene and methanol so as to obtain the glucan bioseparation and purification medium. The method only needs one step of reaction, substantially reduces influence factors, has easily controllable reaction process, improves porosity of the medium and uniformity of granularity, enhances static adsorption capacity and accelerates a mass transfer rate, thereby improving purification and separation efficiency; moreover, the preparation method for the medium is simple and stable and suitable for large scale production. Raw materials used for preparation of the medium are fewer and cheaper, so production cost is greatly reduced.

The invention relates to the technical field of waste treatment, and particularly discloses a construction waste separation device. A rack and a box are included. The box is fixed to the rack, the topof the box is opened, a filter frame is in sliding connection in the box, the upper end face of the filter frame is opened, and the filter frame can be limited at the middle portion of the box. A motor is arranged below the box and fixed to the rack, and a rotation shaft of the motor is fixedly connected with a threaded rod. The end, away from the motor, of the threaded rod extends to the position below the filter frame in the box, the threaded rod is rotationally in sealed connection with the box, and a paddle is arranged on the threaded rod in the box. According to the purpose of the construction waste separation device, the problem that in the construction waste separation process, water flow is needed for continuous washing, and consequently the water consumption is large is solved.

The invention discloses a separation and purification method for geniposide and belongs to the technical field of geniposide separation and purification. After green gardenia fruits are juiced, alcohol extraction is conducted with rice wine, dialysis concentration is conducted, then dialysis concentrated liquor is subjected to adsorption decoloring with a macroporous resin column and crystallized with methyl ester, geniposide crystals are re-dissolved in ethyl acetate, concentration is conducted again, finally, the concentrated liquor is added to an ice-water bath, re-crystallized and then dried, and the separation and purification method for geniposide is obtained. It is proved through an instance that operation is easy and convenient, no toxic reagent is added, environment friendliness is achieved, no impurity exists in prepared geniposide powder products, purity is larger than 98%, the product yield is larger than 97%, and the separation and purification method is suitable for industrial production.