163results about How to "Improve separation and purification efficiency" patented technology

The invention discloses a Process for the purification and separation of trehalose, containing enzymolysis reaction which adds saccharify enzyme in enzyme reaction liquor which is getted from the produced trehalose by enzymic conversion method and inoculating 3-10wt% Saccharomyces cerevisiae in saccharify enzyme enzymolysis liquor, fermenting and culturing in the condition of 25-30deg C and pH 5. 5-7. 5, then getting trehalose pure product after centrifuging, hyperfiltration, ion-exchange, condensation, crystallization and desiccation. Comparing with prevenient activated charcoal column chromatography and organolite process, this invention using two-stage remove mixed sugar of enzymolysis liquor, detecting the getted trehalose pure product by HPLC, its purity is as high as 98. 0%. at the same time, this invention has the advantage of high detaching and purifying efficiency, few lose of trehalose, low cost, simple technology and so on, bringing active impel on predigesting productive technology, improving production and reducing production costs of trehalose's produce by enzyme method.

The invention discloses a method for extracting and purifying anthocyanins from blueberry red leaves. The method is characterized in that two kinds of anthocyanins of which the content is close are contained in a blueberry red leaf anthocyanin extract and account for 93.9 to 99.0 percent of the content of the anthocyanins in the extract. The method comprises the following steps of: selecting fresh blueberry red leaves serving as raw materials, removing leaves which have plant diseases and insect pests and rotted leaves, cleaning by using clean water, drying under vacuum, crushing, sieving, extracting, concentrating under reduced pressure, purifying and separating by macroporous resin, concentrating under the reduced pressure, freeze-drying, saturating, dissolving, growing grains at low temperature, recrystallizing and the like to prepare a natural blueberry red leaf pigment product. By the method, the purity of the recrystallized anthocyanins is over 95 percent; and the anthocyanins are high in purity and simple in composition, and the method is low in production cost and high in popularization.

The invention aims at the field of chemical industry and relates to a separation and purification system of organic synthesis feed liquid and a separation and purification method of the organic synthesis feed liquid. The separation and purification system comprises a crystallization system and a solid-liquid separation system which are sequentially arranged from front to back, wherein the crystallization system comprises a mixed suspension-mixed product-removal (MSMPR) crystallizer and a draft-tube-baffled (DTB) crystallizer which are connected with each other in series; the separation and purification system also comprises a liquid processing system used for processing waste liquid produced by the solid-liquid separation system; the liquid processing system comprises a desalting device and a membrane concentration device which are sequentially arranged from front to back. By virtue of the separation and purification system of the organic synthesis feed liquid and the separation and purification method thereof, the organic synthesis feed liquid is separated and purified by using a continuous crystallization technology, an electrodialysis technology and a membrane separation and concentration technology; the produced waste liquid is fully recycled and used, so that the separation and purification efficiency is improved, the purity and the yield of products are improved and the discharge of the waste liquid is reduced; the separation and purification system of the organic synthesis feed liquid and the separation and purification method thereof are strong in production industrialization and low in costs, and are especially suitable for producing iminodiacetonitrile.

The invention provides the gene of an antibacterial peptide. The gene is used for coding the antibacterial peptide with an amino acid sequence represented by SEQ ID No.1, and possesses one of the nucleotide sequences represented by SEQ ID No.2-4. The invention also provides a preparation method of the antibacterial peptide. The preparation method comprises following steps: (1) an expression system containing one of the nucleotide sequences represented by SEQ ID No.2-4 is constructed, wherein expression carriers are constructed, and cell transformation of host cells is realized by the expression carriers so as to obtain recombinant cells capable of realizing expression of the antibacterial peptide; (2) the recombinant cells are cultured so as to realize expression of the antibacterial peptide; and (3) the expression products are separated and purified so as to obtain the antibacterial peptide with the amino acid sequence represented by SEQ ID No.1. The antibacterial peptide can be used as an antibacterial agent in the fields of agriculture, food, hygienic products, medicine, cosmetic, biopesticide, biological feed additive, natural food antiseptics, and zoological and botanical resistance gene engineering.

The invention discloses a method for separating and preparing anti-tumor components ganoderic acid C1 and ganoderic acid F, which comprises the following steps: (1) primarily enriching and purifying the alcohol extract of lucid ganoderma by use of macroporous resin, and separating a ganoderic acid part with purity of 30-60% from the alcohol extract of lucid ganoderma; (2) separating the separated ganoderic acid part through high-speed counter-current chromatography to obtain ganoderic acid with relatively high purity, wherein a solvent system consists of petroleum ether, ethyl acetate, methanol and water at a volume ratio of 5:(3-7):(1-6):(4-9); the upper phase of the solvent is a stationary phase, and the lower phase is a mobile phase; a sample is dissolved with the lower phase solution and fed; the flow velocity of the stationary phase is 10-20mL/min, the flow velocity of the mobile phase is 1.5-3mL/min, and the rotation speed of the host is 600-1,000rpm; the separated object is collected by an automatic fraction collector, and one fraction is collected from every 4-10mL; (3) analyzing and detecting the components of the liquid collected by each test tube by high performance liquid chromatography, collecting, concentrating and drying according to different components, and separating to obtain ganoderic acid C1 and ganoderic acid F with purity up to 95%. The method has the characteristics of high efficiency, high speed, large separation quantity, high recovery rate, solvent saving and the like.

The invention relates to an extraction and separation method for geniposide and gardenia yellow with high yield and high purity. The invention provides a highly-efficient mild aqueous two-phase technology to realize synchronous separation and purification of the geniposide and the gardenia yellow. The method comprises the following steps: preparing gardenia extract by using gardenia fruit powder as a raw material, then subjecting the obtained gardenia extract to extraction and separation through a multistage low-molecular alcohol-salt aqueous two-phase system, carrying out concentration and drying so as to obtain primary geniposide and gardenia yellow crystals, and carrying out recrystallization so as to obtain geniposide crystal with purity of more than 98.5% and gardenia yellow crystal with a color value of 535. The method provided by the invention has the advantages of simple operation, greenness, high efficiency, economy, time conversation and facilitation to enlargement. Meanwhile, the method initiates application of an aqueous two-phase extraction technology in synchronous separation of the gardenia yellow and the geniposide, and improves separation and purification efficiencies of the geniposide and the gardenia yellow.

The invention relates to a method for separating and purifying geniposide by using an isopropanol-salt double-aqueous-phase system. The invention provides an efficient and gentle method for separating and purifying geniposide by using a double-aqueous-phase technique. The method comprises the following steps: producing a waste gardenia yellow liquid from a cape jasmine fruit raw material, performing extraction separation and concentration drying on the waste gardenia yellow liquid by using the isopropanol-salt double-aqueous-phase system, thereby obtaining a primary geniposide crystal product, and further recrystallizing, thereby obtaining geniposide crystal of which the purity is greater than 98.5%. By adopting the method, a natural geniposide product with high purity is separated and purified from a medical resource for both food and medicine. The method is simple and convenient to operate, environmentally friendly, high in efficiency, economical, time-saving and easy to amplify. The method is not only used for widening the double-aqueous-phase system for extracting geniposide and improving the efficiency in separating and purifying geniposide, but also is used for extracting and producing other pigments.

The invention provides a method for purifying lysine, comprising the following steps of: (1) contacting a lysine-containing solution with a cation exchange resin for carrying out ion exchange to ensure that the lysine is adsorbed onto the cation exchange resin, wherein the pH value of the lysine-containing solution is 3.5-6; (2) eluting the cation exchange resin adsorbed with the lysine in the step (1) with an eluent to ensure that the lysine-containing solution adsorbed onto the cation exchange resin by the eluent is replaced so as to obtain a cleaning solution; (3) eluting the lysine adsorbed onto the cation exchange resin in the step (2) with ammonia water to obtain a lysine eluent; and (4) regenerating resin by contacting the cation exchange resin eluted with the ammonia water in the step (3) with a sulphuric acid solution, and reusing the regenerated cation exchange resin in the step (1). According to the method provided by the invention, the exchange adsorption capacity of the lysine and the cation exchange resin can be greatly improved.

The invention relates to a recycling system and a recycling method for waste liquor produced in separation and purification of an organically synthesized feed liquid, and belongs to the field of chemical engineering. The recycling system for the waste liquor comprises a filter device, a desalting device and a membrane concentrating device which are arranged from the front to the rear in sequence, or the filter device is replaced with a filtrate collecting slot, wherein the filter device is used for filtering and collecting the waste liquor; the filtrate collecting slot is used for stewing and collecting the waste liquor; the desalting device is used for desalting the collected waste liquor; the membrane concentrating device is used for concentrating a liquid which is desalted by virtue of the desalting device; and the desalting device is preferably an electrodialysis device. According to the recycling system and the recycling method, a membrane separating and concentrating technology, an electrodialysis technology and the like are comprehensively utilized to separate and purify the organically synthesized feed liquid, and the produced waste liquor is sufficiently recycled, the separating and purifying efficiency and the purity and the yield of products are improved, discharge of the waste liquor is reduced, the production industrialization is strong and the cost is low, and thus, the recycling system and the recycling method are especially suitable for being applied in production of iminodiacetonitrile.

The invention relates to a method for separation of peptide compounds and concretely relates to a method for separation of palmitoyl pentaspeptide-3 by high performance liquid chromatography. The method mainly solves the problem that in the palmitoyl pentapeptide-3 separation, because of small polarity, a lot of an organic solvent is consumed. The method comprises 1, preparing a chromatographic column used in the high performance liquid chromatography, wherein a silica gel column is used as a stationary phase, 2, selecting chromatographic grade acetonitrile as a mobile phase A, and a mobile phase B with a volume ratio of trifluoroacetic acid to methanol or ethanol of 0.001: 1, 3, selecting a mobile phase A gradient range of 5% to 70%, 4, selecting the detection wavelength of 230nm, and 5, selecting an acetate aqueous solution with a mass percent of 0.5% and chromatographic grade acetonitrile as salt transfer mobile phases. The method reduces the retention time of palmitoyl pentaspeptide-3 so as to improve the purification efficiency.

A process for preparing the extract from capejasmine fruit by use of membrane separation technique includes such steps as extracting in hydrophilic solvent to obtain coarse extract, adding water to become suspension, and membrane separation for removing protein, polyose and other macromolecular impurities.

The invention discloses an efficient contrast agent synthesizing method. The method includes the following steps of firstly, preparing an intermediate mixture of a compound shown in the formula (II) and a compound shown in the formula (I) and/or (III); secondly, conducting separating to obtain the compound shown in the formula (II) and the compound shown in the formula (I) and/or (III); thirdly, taking the compound shown in the formula (II) to prepare a contrast agent (iopromide); fourthly, taking the compound shown in the formula (III) to prepare a contrast agent (iobitridol), and/or taking the compound shown in the formula (I) to prepare a contrast agent (iohexol, ioversol, iopentol or iodixanol). In the method, the iodic contrast agent is prepared by synthesizing and separating intermediates in the formula (II) and the formula (I) and/or the formula (III) and using the intermediates as the raw materials, the problem that diacylation byproducts need to be removed in an existing method is effectively solved, all the intermediates are effectively used, efficiency is high, and the actual application prospects are good. The formulas (I), (II) and (III) can be seen in the description.

The invention discloses a separation and purification method of an ibrutinib intermediate-(3R)-4-amino-3-(4-phenoxyphenyl)-1-(1-tert-butoxycarbonylpiperidine-3-group)-1-1H-pyrazole [3, 4-d] pyrimidine. The separation and purification method includes: after Mitsunobu reaction for preparing the intermediate stops, adding magnesium chloride into a mixture; cooling after back-flowing; filtering to remove compound precipitate of magnesium chloride and triphenyl phosphine oxide to obtain the intermediate high in purity. Column chromatography is omitted in the process, so that the separation and purification method is high in efficiency and low in cost.

The invention discloses an extraction separation preparation method of lignin monomers in schisandra chinensis, which comprises the following steps: performing CO2 supercritical extraction of schisandra chinensis medicinal material powder, performing separation of crude extract by high-speed countercurrent chromatography, performing gradient elution, wherein the high-speed countercurrent solvent system A comprises n-hexane, ethyl acetate, methanol and water, fully mixing, standing, performing elution with the upper phase A as a stationary phase A and the bottom phase A as a mobile phase A to obtain schisandrol A and schisandrol B, changing the solvent system, increasing the volume ratio of methanol to water, performing elution with the bottom phase B as a mobile phase B to obtain schisantherin B; changing the solvent system, further increasing the volume ratio of methanol to water, performing elution with the bottom phase C as a mobile phase C to obtain schisandrin B. According to the invention, the method combines supercritical fluid extraction with high-speed countercurrent chromatography separation; the product can be used directly for subsequent monomer separation and purification without complicated sample treatment after extraction; 5 lignin monomers can be obtained by only separation once; the method is simple and high-efficient.

The present invention discloses a method for producing cis-decahydronaphthalene through naphthalene hydrogenation. According to the method, a fixed bed reactor is adopted; naphthalene and hydrogen-containing gas enter the reactor; in the presence of a hydrogenation catalyst, a hydrogenation reaction is performed under a hydrogenation reaction condition to obtain the cis-decahydronaphthalene, wherein the hydrogenation catalyst comprises a ZSM-5 molecular sieve, an alkali metal, a VIII group metal, an auxiliary agent and silicon dioxide. With the method of the present invention, the specific catalyst and the suitable reaction condition are adopted, such that the content of the cis-decahydronaphthalene produced through naphthalene hydrogenation can be more than 60 wt%, separation purification efficiency can be significantly improved, and target product production cost can be reduced.

The invention relates to a preparation method for a glucan bioseparation and purification medium. The preparation method comprises the following steps: adding glucan into water, carrying out heating, stirring and dissolving and then adding an aqueous sodium hydroxide solution so as to obtain an alkaline glucan solution; adding a cross-linking agent into an oil phase and carrying out heating, stirring and dissolving; and rapidly adding the alkaline glucan solution into the oil phase, carrying out heating and violent stirring so as to obtain emulsion droplets with a uniform particle size of 20 to 120 mu m, slowly adding chloropropylene oxide into the emulsion for a reaction, carrying out rapid cooling and then carrying out cleaning with toluene and methanol so as to obtain the glucan bioseparation and purification medium. The method only needs one step of reaction, substantially reduces influence factors, has easily controllable reaction process, improves porosity of the medium and uniformity of granularity, enhances static adsorption capacity and accelerates a mass transfer rate, thereby improving purification and separation efficiency; moreover, the preparation method for the medium is simple and stable and suitable for large scale production. Raw materials used for preparation of the medium are fewer and cheaper, so production cost is greatly reduced.

The invention discloses a separation and purification method for geniposide and belongs to the technical field of geniposide separation and purification. After green gardenia fruits are juiced, alcohol extraction is conducted with rice wine, dialysis concentration is conducted, then dialysis concentrated liquor is subjected to adsorption decoloring with a macroporous resin column and crystallized with methyl ester, geniposide crystals are re-dissolved in ethyl acetate, concentration is conducted again, finally, the concentrated liquor is added to an ice-water bath, re-crystallized and then dried, and the separation and purification method for geniposide is obtained. It is proved through an instance that operation is easy and convenient, no toxic reagent is added, environment friendliness is achieved, no impurity exists in prepared geniposide powder products, purity is larger than 98%, the product yield is larger than 97%, and the separation and purification method is suitable for industrial production.