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Mycose separation and purification method

A technology for separation and purification of trehalose, applied in the field of separation and purification of trehalose, can solve problems such as unsuitable industrial production, and achieve the effects of low cost, less trehalose loss, and high separation and purification efficiency

Inactive Publication Date: 2006-07-26
胡耀辉
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Problems solved by technology

However, the recovery rate of trehalose in this method is only about 49.8%, which is not suitable for the requirements of industrial production.

Method used

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Embodiment Construction

[0030] A method for separating and purifying trehalose, including the following steps:

[0031] a. Provide the enzyme reaction solution obtained by the enzyme conversion method to produce trehalose and pass it:

[0032] The enzyme reaction solution can be made of starch, starch hydrolysate (maize starch hydrolysate), malto-oligosaccharides, maltose, etc., through enzymes (malto-oligosaccharyl trehalose synthase MTSasc and malto-oligosaccharyl trehalose hydrolase MTHase ​​and The enzyme reaction solution obtained by the action of Trehalose synthase, etc.). After determination, the trehalose content of the enzyme reaction solution accounts for about 60% of the total sugar content. The enzyme reaction solution is passivated at 100°C for 10 minutes to make the enzyme in the reaction solution Denaturation of similar and heat-insensitive proteins;

[0033] b. Glucoamylase hydrolysis reaction:

[0034] According to the sugar content in the enzyme reaction solution and the conversion rate o...

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Abstract

The invention discloses a Process for the purification and separation of trehalose, containing enzymolysis reaction which adds saccharify enzyme in enzyme reaction liquor which is getted from the produced trehalose by enzymic conversion method and inoculating 3-10wt% Saccharomyces cerevisiae in saccharify enzyme enzymolysis liquor, fermenting and culturing in the condition of 25-30deg C and pH 5. 5-7. 5, then getting trehalose pure product after centrifuging, hyperfiltration, ion-exchange, condensation, crystallization and desiccation. Comparing with prevenient activated charcoal column chromatography and organolite process, this invention using two-stage remove mixed sugar of enzymolysis liquor, detecting the getted trehalose pure product by HPLC, its purity is as high as 98. 0%. at the same time, this invention has the advantage of high detaching and purifying efficiency, few lose of trehalose, low cost, simple technology and so on, bringing active impel on predigesting productive technology, improving production and reducing production costs of trehalose's produce by enzyme method.

Description

Technical field [0001] The invention relates to a method for separating and purifying trehalose, in particular to a method for separating and purifying trehalose in the process of producing trehalose by using an enzyme conversion method. Background technique [0002] Trehalose is a non-reducing disaccharide formed by the combination of two glucose molecules through α,α-1,1 bonds. Because trehalose has a non-reducing end, it has the function of protecting biological cells and biologically active substances from being destroyed under adverse environmental conditions such as dehydration, drought, high temperature, freezing, high osmotic pressure, and toxic reagents. Therefore, it has been affected in recent years. People are paying more and more attention. [0003] At present, the production methods of trehalose mainly include microbial extraction, fermentation, enzyme conversion and genetic engineering. Among them, the enzyme conversion method has broad application prospects due to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/04C12S3/00
Inventor 胡耀辉朴春红于寒松彭帅刘俊梅刘仁杰王欢
Owner 胡耀辉
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