Two-aqueous-phase extraction method of polygonatum sibiricum polysaccharide

A Polygonatum polysaccharide, two-phase technology, applied in the field of two-phase extraction of Polygonatum polysaccharide, can solve the problems of high cost, low efficiency, and low product purity, and achieve the effect of low cost, high efficiency, and high purity of the separated product

Active Publication Date: 2020-05-22
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
View PDF3 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problems of low efficiency, high cost and low product purity in the existing Polygonatum polysaccharide separation and purification method, the present invention provides a two-water phase extraction method for separating and purifying Polygonatum polysaccharide

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Two-aqueous-phase extraction method of polygonatum sibiricum polysaccharide
  • Two-aqueous-phase extraction method of polygonatum sibiricum polysaccharide
  • Two-aqueous-phase extraction method of polygonatum sibiricum polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0027] (1) After the Polygonatum raw material is pulverized, pass through a 50-mesh sieve, use petroleum ether to degrease and dry, and extract Polygonatum Polysaccharide with water to obtain a concentration of 50g / L Polygonatum polysaccharide extract, wherein the extraction temperature is 80°C, and the solid-to-liquid ratio is 1: 20, stirring rate 400r / min, extraction time 2h.

[0028] (2) Adding 49g mass fraction to 40.8% ammonium sulfate aqueous solution in 20g dehydrated ethanol obtains a two-phase extraction system, and the ethanol-ammonium sulfate two-phase phase diagram is as follows figure 2 shown.

[0029] (3) Take 31g Polygonatum polysaccharide extract and add it into the ethanol-ammonium sulfate two-phase extraction system, the extraction temperature is 40°C, the stirring speed is 400r / min, and the extraction time is 10min. After the extraction, the liquid was separated and the lower phase was kept.

[0030] (4) Add 5 times of absolute ethanol to the volume of th...

specific Embodiment approach 2

[0033] (1) After the Polygonatum raw material is pulverized, pass through a 50-mesh sieve, use petroleum ether to degrease and dry, and extract Polygonatum Polysaccharide with water to obtain a concentration of 50g / L Polygonatum polysaccharide extract, wherein the extraction temperature is 80°C, and the solid-to-liquid ratio is 1: 20, stirring rate 400r / min, extraction time 2h.

[0034] (2) Adding 46g mass fraction to 39.13% ammonium sulfate aqueous solution in 25g dehydrated ethanol, obtains two-phase extraction system, and ethanol-ammonium sulfate two-phase phase diagram is as follows figure 2 shown.

[0035] (3) Take 29g Polygonatum polysaccharide extract and add it into the ethanol-ammonium sulfate two-phase extraction system, the extraction temperature is 40°C, the stirring speed is 400r / min, and the extraction time is 10min. After the extraction, the liquid was separated and the lower phase was kept.

[0036] (4) Add 5 times of absolute ethanol to the volume of the tw...

specific Embodiment 3

[0039] Embodiment 3 differs from Embodiment 1 in that the Polygonatum polysaccharide extract is used in an amount of 26 g, and the amount of absolute ethanol is 25 g, and the rest of the treatment and processing methods are the same as in Embodiment 1. The yield of Polygonatum Polysaccharide obtained is 34.8%, and the purity is 85%.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a two-aqueous-phase extraction method of polygonatum sibiricum polysaccharide, and belongs to the technical field of bioengineering. The method solves the problems of low efficiency, high cost and low product purity of existing polygonatum sibiricum polysaccharide separation and purification methods. The method comprises the following steps: crushing polygonatum sibiricum raw materials, degreasing, drying, and performing extraction by using a water extraction method to obtain polygonatum sibiricum polysaccharide extract solution; and extracting and separating the polygonatum sibiricum polysaccharide by adopting a small-molecular organic solvent-soluble salt two-aqueous-phase extraction system, concentrating under reduced pressure, and drying to obtain a purified polygonatum sibiricum polysaccharide product. According to the method, the polygonatum sibiricum polysaccharide is extracted and separated by adopting the small-molecular organic solvent-soluble salt two-aqueous-phase extraction system, so that the separation and purification efficiency is greatly improved, the production cost is reduced, soluble impurities in the polygonatum sibiricum polysaccharideare effectively removed, and the product purity is improved; and the raffinate contains a small amount of polygonatum sibiricum polysaccharide, can be separated and purified again after being recovered, and the method is low in cost and convenient for industrial amplification.

Description

technical field [0001] The invention relates to an aqueous two-phase extraction method of Polygonatum Polysaccharides, which belongs to the technical field of bioengineering. Background technique [0002] Polygonatum sibiricum Red, also known as tiger ginger and chicken head ginseng, is a perennial herb belonging to the genus Polygonatum sibiricum in the Liliaceae family. Polygonatum sibiricum polysaccharides, saponins, amino acids, flavonoids, and anthraquinones are the main chemical components of Polygonatum sibiricum polysaccharides (PSP). Polygonatum polysaccharide has many functions such as delaying aging, enhancing human immunity, lowering blood sugar, lowering blood fat, anti-arteriosclerosis, anti-tumor, anti-inflammation, and anti-virus. Polysaccharides, also known as polysaccharides, are polymers formed by the condensation of monosaccharides. They are widely distributed in nature and are an important class of biologically active substances. Polygonatum polysaccha...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003Y02P20/54
Inventor 王建勋张同咸漠董晴晴荀明月杜文宣
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap