Composite magnetic nanoparticles Fe3O4@Au/MPA/NTA-Ni<2+> as well as preparation and application thereof

A nano-particle and composite magnetic technology, which is applied in the field of nano-magnetic materials and biology, can solve the problems of stability and uneven surface modification, and achieve good monodispersity, good dispersion, and rapid separation.

Inactive Publication Date: 2018-08-07
HUBEI UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In order to solve the above problems, the invention provides a composite magnetic nanoparticle Fe 3 o 4 @Au / MPA / NTA-Ni 2+ The preparation and application of histidine-tagged protein separation and purification not only solved the problems of its stability and uneven surface modification, but also greatly improved its separation and purification efficiency and recycling rate

Method used

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  • Composite magnetic nanoparticles Fe3O4@Au/MPA/NTA-Ni&lt;2+&gt; as well as preparation and application thereof
  • Composite magnetic nanoparticles Fe3O4@Au/MPA/NTA-Ni&lt;2+&gt; as well as preparation and application thereof
  • Composite magnetic nanoparticles Fe3O4@Au/MPA/NTA-Ni&lt;2+&gt; as well as preparation and application thereof

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Experimental program
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Effect test

Embodiment 1

[0040] The above-mentioned composite magnetic nanoparticles Fe 3 o 4 @Au / MPA / NTA-Ni 2+ Prepared by the following method, comprising the steps of:

[0041] 1) Preparation of Fe by solvothermal method 3 o 4 Nanoparticles: 1.156g ferric chloride hexahydrate (FeCl 3 ·6H 2 O), 3.3012g ammonium acetate (NH 4 AC), 0.3424g of trisodium citrate dissolved in 60mL of ethylene glycol, heated to 170 ° C for 1 hour; after cooling to room temperature, the solution was transferred to the reaction kettle, and reacted at 200 ° C for 16 hours; after the reaction, magnetic Separation, washing with absolute ethanol and water alternately for 3 times, and finally dispersing in absolute ethanol;

[0042] 2) Fe 3 o 4 Surface modification of nanoparticles: ①Take 1.96g of maleic anhydride (maleic anhydride) in a 10mL round bottom flask, add 3.4mL of aminopropyltriethoxysilane (APTES) dropwise under ice-water bath conditions , put into the rotor and stir until the reactant turns into a white so...

Embodiment 2

[0049] The above-mentioned composite magnetic nanoparticles Fe 3 o 4 @Au / MPA / NTA-Ni 2+ Prepared by the following method, comprising the steps of:

[0050] 1) Preparation of Fe by solvothermal method 3 o 4 Nanoparticles: 1.156g ferric chloride hexahydrate (FeCl 3 ·6H 2 O), 2.312g ammonium acetate (NH 4 AC), 0.8216g of trisodium citrate dissolved in 60mL of ethylene glycol, heated to 170 ° C for 1 h; after cooling to room temperature, the solution was transferred to the reaction kettle, and reacted at 200 ° C for 8 h; after the reaction, magnetic separation was carried out. Alternately wash 3 times with absolute ethanol and water, and finally disperse in absolute ethanol;

[0051] 2) Fe 3 o 4 Surface modification of nanoparticles: ①Take 1.96g of maleic anhydride (maleic anhydride) in a 10mL round bottom flask, add 3.4mL of aminopropyltriethoxysilane (APTES) dropwise under ice-water bath conditions , put into the rotor and stir until the reactant turns into a white soli...

Embodiment 3

[0057] The above-mentioned composite magnetic nanoparticles Fe 3 o 4 @Au / MPA / NTA-Ni 2+ Prepared by the following method, comprising the steps of:

[0058] 1) Preparation of Fe by solvothermal method 3 o 4 Nanoparticles: 1.156g ferric chloride hexahydrate (FeCl 3 ·6H 2 O), 5.78g ammonium acetate (NH 4 AC), 0.2312g of trisodium citrate dissolved in 60mL of ethylene glycol, heated to 170 ° C for 1 h; after cooling to room temperature, the solution was transferred to the reaction kettle, and reacted at 200 ° C for 10 h; after the reaction, magnetic separation was carried out. Alternately wash 4 times with absolute ethanol and water, and finally disperse in absolute ethanol;

[0059] 2) Fe 3 o 4 Surface modification of nanoparticles: ①Take 1.96g of maleic anhydride (maleic anhydride) in a 10mL round bottom flask, add 3.4mL of aminopropyltriethoxysilane (APTES) dropwise under ice-water bath conditions , put into the rotor and stir until the reactant turns into a white soli...

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Abstract

The invention belongs to the technical field of nano magnetic materials and biology and in particular relates to composite magnetic nanoparticles Fe3O4@Au / MPA / NTA-Ni<2+> as well as preparation and application thereof. BSA (Bull Serum Albumin) surface sulfhydrylation magnetic Fe3O4 nanoparticles and gold nanoparticles are self-assembled under the action of a disulfide bond to form superparamagneticFe3O4@Au nanoparticles; after surfaces of the superparamagnetic Fe3O4@Au nanoparticles are carboxylated by adopting MPA (3-mercaptopropionic acid), coupling with an affinity ligand NTA-Ni<2+> throughan amide bond effect; the prepared Fe3O4@Au / MPA / NTA-Ni<2+> composite magnetic nanoparticles have superparamagnetic performance, the average hydration particle diameter is 100nm to 1000nm, the averageZeta potential is 0 to -25mv and the dispersity is good; when the composite magnetic nanoparticles are applied to protein separation and purification, a process is simple and the separation is rapid;after being circularly used for 6 times, the composite magnetic nanoparticles still have relatively high separation and purification efficiency.

Description

technical field [0001] The invention belongs to the field of nano magnetic materials and biotechnology, in particular to a composite magnetic nano particle Fe 3 o 4 @Au / MPA / NTA-Ni 2+ and its preparation and application. Background technique [0002] With the development of biotechnology, the preparation of protein drugs by recombinant technology has become a research hotspot. In the research of protein drugs, the separation and purification of proteins is the most critical part. The efficiency and cost of separation and purification directly affect the development of protein drugs. Therefore, The optimization of protein separation and purification technology is urgent. [0003] Existing protein separation and purification methods include salting out, organic solvents, membrane separation techniques, ion exchange techniques, and affinity chromatography techniques, among which affinity chromatography is based on the interaction between immobilized ligands and proteins with ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/22B01J20/30B01J20/28C07K1/14
CPCB01J20/02B01J20/06B01J20/22B01J20/28009B01J2220/46B01J2220/4806B01J2220/4812C07K1/14
Inventor 郭惠玲孙宏浩刘明星祝红达孙红梅
Owner HUBEI UNIV OF TECH
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