A method for optically and ultrasonically acquiring the information about biological tissue and chromatographic imaging includes ultrasonically scanning the biologic tissue to find out possible lesion position, emitting the laser pulses and ultasonic waves to the tissue at lesion position to obtain photoultrasonic and reflected ultrasonic signals, receiving the ultrasonic echo and photo-acoustical signals, data processing by computer, and straight projection for chromatographic imaging. Its equipment is composed of laser, multi-element ultrasonic array, externally triggered signal source, data acquisition card, delay circuit, and computer.