220results about How to "Simplified extraction steps" patented technology

The invention discloses a lane line extraction method, apparatus and system, relates to the technical field of traffic, and mainly aims to improve lane line extraction precision and efficiency. The method comprises the steps of collecting a measureable real scene road image; by analyzing the measureable real scene road image, constructing a grid dynamic window-based lane identification region of interest, and performing lane line edge detection on the lane identification region of interest; and extracting lane lines according to lane line information obtained by detection and a lane line extraction model subjected to line fitting correction in a preset lane. The lane line extraction method, apparatus and system is suitable for measureable real scene road image-based lane line extraction.

The invention discloses a method for extracting the width of river channels within a basin by using a digital elevation model. The method comprises the following main steps: the digital elevation model (DEM) data of the basin are extracted; the slope grids, river channel grids and confluence accumulative grids of the basin are calculated according to the digital elevation model (DEM) data of the basin; near-river channel slope grids are extracted by using the slope grids and the river channel grids; near-river channel confluence accumulative grids are extracted by using the confluence accumulative grids and the river channel grids; moving average calculation is performed on the basis of the near-river channel slope grids, so that moving averaged near-river channel slope grids can be obtained; and the width of the river channels within the basin is extracted on the basis of the near-river channel confluence accumulative grids and the moving averaged near-river channel slope grids. The method of the invention has the advantages of stable and reliable data sources, high calculation efficiency, objective and reasonable results and the like. With the method adopted, the rapid calculation and extraction of the width of the river channels within the basin can be benefitted.

A device and method for extracting micro-plastic in settlings are provided. The device includes a filter drum, a liquid storage tank, a motor, a pedestal, a screen sieve and a transmission shaft. The filter drum is mounted in the liquid storage tank. The upper port of the filter drum is provided with the screen sieve. The liquid storage tank and the motor are mounted on the pedestal. The bottom of the liquid storage tank is provided with a drainage hole communicated with the filter drum. The motor is connected to one end of the transmission shaft through a rotating shaft, and the other end of the transmission shaft passes through the drainage hole and is connected to the bottom of the filter drum. The device and the method are advantageous in that (1) dispersion effects of muddy settlings and elution effects of a sample are obviously improved; (2) the recovery rate of the sample is increased, (3) the extraction efficiency is greatly increased, (4) extraction time is shortened and (5) labor is saved.

The present invention discloses a strain of Bacillus methylotrophicus VJ4-1, and a method for producing natural vanillin by ferulic acid biotransformation with the Bacillus methylotrophicus VJ4-1, wherein a name of the strain is Bacillus methylotrophicus VJ4-1, the strain is preserved in the China center for type culture collection (CCTCC) in Wuhan University on January 16, 2012, and a preservation number is CCTCC NO:M2012004. The method of the present invention has advantages of high yield, simple process, mild reaction conditions, less environmental pollution, high product concentration, simple extraction step, clean production, environmentally friendly products, safety, reliability, wide main raw material source, low cost, and natural property, and has an industrial production prospect.

The invention discloses a mountain torrent rapid early warning and forecasting method based on multi-dimensional rainfall characteristic space optimal decision. The method comprises: firstly, calculating initial soil water saturation S of a successive historical rainfall flood process based on a three-layer evapotranspiration principle; secondly, counting the rainfall P of the successive historical rainfall flood process; thirdly, quantifying a rainfall space distribution characteristic K of a successive historical rainfall flood process by combining drainage basin DEM data; calculating a landform unit line of the drainage basin based on the DEM data; taking P, K and S as mutually orthogonal coordinate axes to construct a multi-dimensional rainfall characteristic space coordinate system, establishing a rainfall data point set, dividing the corresponding rainfall data point set into two types of an ultra-alarm subset A and a non-ultra-alarm subset B according to whether the flood process is over-alarm or not, and solving an optimal classification decision equation between the two types of subsets by combining an SVM principle; and if early warning is needed, calculating the runoff process at the outlet of the drainage basin by taking the rainfall as the input of a landform unit line, and carrying out real-time flood forecasting.

The invention discloses a method for extracting DNA (Deoxyribonucleic Acid) of a rice leaf blade or a rice seed rapidly and efficiently by using a 96 deep-hole plate and a mixer mill, relating to the field of biology. The method is a rapid and effective plant DNA extraction method, the whole extraction course is free from single-shotgun operation but undergoes stranded-shotgun operation completely, and in addition, serial numbers do not need to be written, so that a rapid high-flux extraction method is realized. The method can be applied to rice molecular breeding, purity identification, seed authenticity detection, gene cloning and the like.

The invention discloses a system for inducing foreign genes to express in gram-negative bacteria and application of the system. The invention provides a method for expressing the foreign genes in the gram-negative bacteria. According to the method, a regulating and control element comprising a repressor protein expression box and an RNA (ribonucleic acid) polymerase expression box is used for regulating and controlling the expression of a foreign gene expression box in the gram-negative bacteria. Experiments prove that the system for inducing the foreign genes to effectively express in the gram-negative bacteria provided by the invention consists of two parts including specific polymerase and a corresponding promoter; the polymerase, the promoter and driven foreign genes are induced to express in the gram-negative bacteria; the efficient mass expression of the foreign genes can be realized; the important significance of simplifying the extraction step, improving the protein expression quantity and reducing the production cost are realized in industrial production; wide application prospects are realized; wide commercial utilization values are realized.

The invention provides a method for extracting Sargassum furiforme polysaccharides, which has the advantages of simple operation steps, low energy and material consumption, higher polysaccharide content and higher purity. The method comprises the following steps: (1) drying Sargassum furiforme, pulverizing the Sargassum furiforme into powder, and passing the powder through a 40-200-mesh screen; (2) thoroughly mixing the Sargassum furiforme alga powder obtained in the step (1) with 95% ethanol, and carrying out reflux degreasing; (3) recycling the degreased alga powder in the step (2), adding distilled water, filtering through bolting silk several times, collecting the filtrates, merging the filtrates, and concentrating under reduced pressure to obtain a concentrated solution; (4) adding 95% ethanol into the concentrated solution in the step (3), carrying out alcohol precipitation, centrifugating, collecting the precipitate, adding distilled water, and stirring until the solid is completely dissolved; and (5) carrying out freeze vacuum drying on the polysaccharide solution in the step (4) to obtain the polysaccharide product.

The invention relates to the technical field of biologics, in particular to a method for extracting a toxin protein which causes vascular permeability increase from white cyanea tentacles. The method comprises the following steps: unfreezing fresh white cyanea tentacles which are frozen at a super-low temperature at a low temperature, washing by a small quantity of buffer solution, collecting the buffer solution, centrifuging to obtain a supernatant and using the supernatant as cyanea crude toxin, and sequentially separating by an ion chromatographic column and a gel chromatographic column to obtain the toxin protein CnP45 which causes vascular permeability increase, wherein the molecular weight of the toxin protein is 45kD. The extracting process of the jellyfish toxin is simplified, the interference of impurity proteins is reduced, and an important method instruction is provided for the further separation and purification of jellyfish toxin ingredients.

The invention provides a layout characteristic parameter extraction method for graphic data of a grid point edge region. The method comprises the steps of performing grid point division on original design layout data for at least four times, and during the grid point division, expanding all edges of a grid point from inside to outside of a graph to form an annular region; distinguishing a graph in the grid point from an edge graph of the grid point; calculating the weight line width, perimeter and density of the graph in the grid point to obtain calculation results and influence factor localization; calculating the weight line width, perimeter and density of the graph in the grid point edge region to obtain calculation results and influence factor localization; and calculating geometric characteristic parameter values of all grid points.

The invention relates to the technical field of detection of baijiu ingredients, in particular to a method for determining the squalene content of baijiu. According to the method, conditions such as the extraction stage number, the dilution of a liquor sample, types of organic solvents, chromatographic column selection and the like are optimized, and the method combining multistage-LIME (liquid-liquid micro-extraction) and GC-MS (gas chromatography-mass spectrometry) for determining the squalene content of baijiu is established. The method has the advantages of simplicity, convenience, accuracy, sensitivity and rapidness, the limit of detection of the squalene is 0.17 mu g/L, the limit of quantitation is 0.55 mu g/L, the determined recovery rate of the squalene in 11 types of baijiu ranges from 85.12% to 113.64%, the relative standard deviation is smaller than 13.80%, and quantitative analysis of trace triterpene with biological activity and with the content of ng/L or mu g/L level in baijiu can be realized.

The invention provides an extraction method of natural plant polysaccharide and belongs to the field of natural product preparation. The extraction method comprises the steps of inoculating enzyme-producing bacteria capable of secreting cell wall degrading enzyme to an enzyme-producing fermentation culture medium, then adding natural plant powder for incubating and fermenting, mixing an obtained fermentation liquid with an alcohol solution, and conducting precipitation. The extraction method greatly simplifies the extraction steps of the natural plant polysaccharide, reduces the extraction cost, improves the efficiency of extracting the natural plant polysaccharide, avoids the use of toxic and harmful reagents including ether, chloroform and the like, and is beneficial to mass applicationof the natural plant polysaccharide in animal husbandry.

The invention discloses a chicken whole blood simple, convenient and quick DNA extraction method which comprises the following steps of after adding chicken whole blood in an anticoagulant, adding a mixture in lysate for mixing, putting a mixture in a water solution boiler with the temperature being 56-60 DEG C for 20-30min, adding a separating medium in a product, putting a mixture in the water solution boiler with the temperature being 70-80 DEG C for 20-30min for centrifugation, taking a supernate, transferring the supernate into another EP pipe, adding a precipitated solution, performing centrifugation, reserving precipitates, adding a washing solution, and performing uniform mixing and centrifugation; and carefully discarding the supernate, collecting the precipitates, drying the precipitates at the room temperature, and adding a TB buffer liquid to obtain a chicken DNA extracting solution. By adopting the chicken whole blood simple, convenient and quick DNA extraction method provided by the invention, the purity and the integrity of extracted DNA can both reach test requirements, and the concentration and the purity are superior to that of DNA extracted by an existing kit. Bycombining with application on a chicken molecular breeding aspect, proved that the method is an environment-friendly DNA extraction method with low cost and simple operation.

The invention discloses a method for searching a similar watershed based on a spatial dimension division theory. The method comprises the following steps: firstly, dividing a drainage basin into a plurality of orthogonal grid units based on DEM data to obtain an elevation in each grid unit, thereby obtaining an elevation grid Raster_Ele of the drainage basin, and calculating to obtain an elevationfractal dimension value Fra_Ele; secondly, calculating the gradient in each grid unit to obtain a gradient grid Raster_Slope of the drainage basin, and calculating to obtain a gradient fractional dimension value Fra_Slope; next, calculating the slope direction in each grid unit to obtain a slope direction grid Raster_Slope Dir of the drainage basin, and calculating to obtain a slope direction sub-dimension value Fra_Slope Dir; and finally, constructing a multi-dimensional feature space, generalizing a drainage basin into data points, labeling the data points in the multi-dimensional feature space, and classifying the data points, so that the drainage basin divided into one class is regarded as a similar drainage basin.

The invention discloses a metagenome extraction method for specifically reducing or removing a host genomic DNA based on a CRISPR-Cas system composition. The invention aims to provide a technique forreducing a host genomic DNA background in metagenome sequencing and improving effective sequencing data of other microorganisms. The CRSIPR-Cas system in the method comprises a plurality of crRNA or crRNA/tracrRNA derivatives, as well as a biotin-labeled Cas protein or variant protein with endonuclease activity deficiency. The sequence of the crRNA or crRNA/tracrRNA derivatives is complemented with a repetitive sequence Alu element region (target sequence) in the host genomic DNA, so that the binding of the Cas protein or the variant thereof with endonuclease activity deficiency is guided to be bonded on the host genomic DNA, the CRISPR-Cas system and the bonded host DNA are captured by adding magnetic beads coated with streptavidin, and thus the concentration of the host genomic DNA in asample solution is reduced and the abundance of nucleic acids of other non-host genomic DNA is improved.

The invention discloses a method for extracting high-activity immunoglobulin in blood, belonging to the range of food and feed additive and bio-chemical detection reagent preparation technology. Said invention is aimed at overcoming the defect of the existent technique and provides the method adopting low-temp. organic solvent and combining it with ultrafiltration process. Said method includes the following steps: firstly, using low-temp. organic solvent to extract immunoglobulin in the blood, then ultrafiltering and purifying immunoglobulin, finally freze-drying or low-temp. spray-drying so as to obtain the invented product. Its immunoglobulin content can be up to above 65%. Said invention can simplify production steps of low-temp. ethyl alcohol precipitation method, can reduce denaturing rate of immunoglobulin, and can recover albumin and ethyl alcohol and can fully recover and utilize wastes.

The invention belongs to the technical field of fungal hypha total DNA extraction, and particularly relates to a fungal hypha total DNA extracting solution and a method for extracting fungal hypha total DNA. The fungal hypha total DNA extracting solution comprises an extracting solution I and an extracting solution II, wherein the extracting solution I comprises a buffer salt, a nonionic surfactant and a neutral salt water solution, and the pH value of the extracting solution I is 7.5-8.5; and the extracting solution II comprises a buffer salt and a cationic surfactant, and the pH value is 7.5-8.5. The fungal hypha total DNA extracting solution simplifies the total DNA extraction steps, is suitable for extracting trace amounts of total DNA, provides an effective experimental means for the scarce experimental materials, avoids the use of the toxic organic solvents, can simplify the operation steps and shorten the time, and avoids the possibility of high tendency to cause frost damage to skin in the liquid nitrogen grinding process.