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Extraction method of natural plant polysaccharide

A natural plant and extraction method technology, applied in the field of natural product preparation, can solve the problems of restricting the popularization and use of natural plant polysaccharides, complex extraction steps, and high cost, and achieve the goal of reducing extraction costs, simplifying extraction steps, and increasing extraction volume Effect

Inactive Publication Date: 2019-04-12
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this type of extraction method, there are complex extraction steps, high cost, and the use of toxic and harmful reagents such as chloroform, ether, n-butanol, etc., which seriously restricts the large-scale promotion and use of natural plant polysaccharides in animal husbandry.

Method used

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  • Extraction method of natural plant polysaccharide
  • Extraction method of natural plant polysaccharide
  • Extraction method of natural plant polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] The present embodiment provides a kind of extraction method of natural plant polysaccharide, it comprises the following steps:

[0069] A. Preparation of enzyme-producing fermentation medium:

[0070] Prepare enzyme-producing fermentation medium according to the following formula: (NH) 2 SO 4 4g, KH 2 PO 4 2g, MnSO 4 ·7H 2 O 0.5g, peptone 10g and beef extract 5g, dilute to 1L with deionized water, adjust the pH of the solution to 7.2, and sterilize at high temperature.

[0071] B. Inoculation and cultivation:

[0072] Inoculate Bacillus licheniformis into the enzyme-producing fermentation medium according to the inoculum amount of 1%, then add sterilized astragalus powder according to the mass volume ratio of 10g / L, mix well, and incubate at 37°C and 220rpm for 48h to obtain fermentation broth.

[0073] C. Extraction of polysaccharides:

[0074] After centrifuging the fermentation broth, take the supernatant, and mix it with anhydrous ethanol solution at a volu...

Embodiment 2

[0076] The present embodiment provides a kind of extraction method of natural plant polysaccharide, it comprises the following steps:

[0077] A. Preparation of enzyme-producing fermentation medium:

[0078] Prepare enzyme-producing fermentation medium according to the following formula: (NH) 2 SO 4 5g, KH 2 PO 4 1g, MnSO 4 ·7H 2 O 0.1g, peptone 7g and beef extract 3g, dilute to 1L with deionized water, adjust the pH of the solution to 7.0, and sterilize at high temperature.

[0079] B. Inoculation and cultivation:

[0080] Bacillus licheniformis was inoculated in the enzyme-producing fermentation medium according to the inoculation amount of 1.5%, and then sterilized astragalus powder was added according to the mass volume ratio of 15g / L, and after mixing, it was incubated at 30°C and 300rpm for 12h to obtain fermentation broth.

[0081] C. Extraction of polysaccharides:

[0082] After centrifuging the fermentation broth, take the supernatant and mix it with 70% etha...

Embodiment 3

[0084] The present embodiment provides a kind of extraction method of natural plant polysaccharide, it comprises the following steps:

[0085] A. Preparation of enzyme-producing fermentation medium:

[0086] Prepare enzyme-producing fermentation medium according to the following formula: (NH) 2 SO 4 3g, KH 2 PO 4 3g, MnSO 4 ·7H 2 O 1g, peptone 13g and beef extract 7g, dilute to 1L with deionized water, adjust the pH of the solution to 7.2, and sterilize at high temperature.

[0087] B. Inoculation and cultivation:

[0088] Bacillus licheniformis was inoculated in the enzyme-producing fermentation medium according to the inoculation amount of 0.5%, and then sterilized astragalus powder was added according to the mass volume ratio of 5g / L, and after mixing, it was incubated at 45°C and 150rpm for 60h to obtain fermentation broth.

[0089] C. Extraction of polysaccharides:

[0090] After centrifuging the fermentation broth, take the supernatant and mix it with 90% ethano...

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Abstract

The invention provides an extraction method of natural plant polysaccharide and belongs to the field of natural product preparation. The extraction method comprises the steps of inoculating enzyme-producing bacteria capable of secreting cell wall degrading enzyme to an enzyme-producing fermentation culture medium, then adding natural plant powder for incubating and fermenting, mixing an obtained fermentation liquid with an alcohol solution, and conducting precipitation. The extraction method greatly simplifies the extraction steps of the natural plant polysaccharide, reduces the extraction cost, improves the efficiency of extracting the natural plant polysaccharide, avoids the use of toxic and harmful reagents including ether, chloroform and the like, and is beneficial to mass applicationof the natural plant polysaccharide in animal husbandry.

Description

technical field [0001] The invention relates to the field of natural product preparation, in particular to a method for extracting natural plant polysaccharides. Background technique [0002] Plant polysaccharides, also known as plant polysaccharides, are polysaccharides with a degree of polymerization of more than 10 produced by plant cell metabolism. Studies have found that plant polysaccharides have obvious growth-promoting effects, improve the efficacy of drugs and the ability of antigen immune response, and can activate immune cells. The main source of plant polysaccharides is extracted from natural plants rich in polysaccharides, that is, natural plant polysaccharides. [0003] The existing extraction methods of natural plant polysaccharides are mainly water decoction extraction method, physical and chemical extraction method and so on. In this type of extraction method, there are complex extraction steps, high cost, and the use of toxic and harmful reagents such as ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 彭军胡栋吴家强王涛徐煜琳朱迎春赵情
Owner SHANDONG AGRICULTURAL UNIVERSITY
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