Method for extracting useful substances from shrimp shells
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A shrimp shell, a useful technology, applied in the field of extracting useful substances, can solve problems such as complex processes, achieve simple extraction steps, good quality, and improve the effect of raw material utilization
Active Publication Date: 2019-08-20
HUNAN BEIBEISHENG BIOTECHNOLOGY CO LTD
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After adding the enzyme solution, an organic solvent must be added. This method is not an all-biological enzymatic hydrolysis method. The organic solvent is still used. The crude enzyme solution may play a catalytic role. It also needs to use resin adsorption and elution, alcohol washing and other procedures. Complex, and there are safety and health issues in the use of organic solvents
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Embodiment 1
[0041] Embodiment 1: a kind of biological enzymatic method extracts the method for astaxanthin and shrimp protein from crayfish shell, comprises the following steps:
[0042] Step 1. Crayfish. Shrimp shells (including shrimp heads and shrimp tails) are pretreated, and 100kg of frozen crayfish shells are minced into 20-50mm pieces with a meat grinder. Step 2. Put the minced shrimp shell fragments in a 1:1 ratio of water in a reaction tank, heat up to 30-40°C, pH value 7.0, add biological alkaline enzyme powder 1000:1, and stir at a constant temperature of 45°C. Rotate at 45-60 rpm, digest for 60 minutes, and get 100kg of enzymatic hydrolyzate. Step 3. Centrifuge the obtained 100kg of enzymatic hydrolysis solution to obtain 17.4kg of astaxanthin-rich shrimp protein slurry. Step 4. Put the 17.4kg of astaxanthin-rich shrimp protein slurry into the digestion tank and add 7kg of vegetable oil , heated to 58°C, added pure water at a temperature of 58.5°C at a mass ratio of 6:4, sti...
Embodiment 2
[0045] Embodiment 2: A method for extracting astaxanthin and shrimp protein from sea shrimp shells by a biological enzyme method, comprising the following steps:
[0046] Step 1. Mince the frozen sea shrimp shells (including shrimp heads and shrimp tails) into 20-50mm pieces.
[0047] Step 2. Put the minced shrimp shell fragments into the reaction tank with 1:1 water, heat up to 30°C to 40°C, and the pH value is 6.8-7.0. Add biological enzyme alkaline enzyme powder 1000:1, constant temperature 45%, stirring speed 50 rpm, digest for 90 minutes, and obtain 100kg enzymolysis solution.
[0048] Step 3, centrifuging 100 kg of the obtained enzymatic hydrolyzate to obtain 16.5 kg of astaxanthin-rich shrimp protein slurry.
[0049] Step 4. Put 16.5kg of astaxanthin-rich shrimp protein slurry into the digestion tank, add 7kg of vegetable oil, stir, heat to 55°C, add pure water at a temperature of 56°C at a mass ratio of 6:4, Stir at constant temperature for 60 minutes, centrifuge to ...
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Abstract
The invention relates to a method for extracting useful substances from shrimp shells. The method comprises the following steps: crushing the shrimp shells, mixing the crushed shrimp shells and water,performing heating to 28-35 DEG C, adjusting the pH to 6.8-7.5, preferably, 6.8-7, adding an alkaline protease, performing mixing, performing heating to 42-48 DEG C, performing constant-temperature enzymolysis for 50-70 min, and performing sieving to obtain an enzymatic hydrolysate and solid residues; performing centrifugal separation treatment on the enzymatic hydrolysate to obtain a shrimp protein deposit containing astaxanthin; mixing the shrimp protein deposit and water, performing heating while stirring, adjusting the pH to 6.8-7.0, performing heating to 58-60 DEG C, adding vegetable oil, and performing emulsification for 50-70 min under stirring to obtain an emulsion; and performing centrifugation on the emulsion, and performing layering to obtain oil containing the astaxanthin at the upper layer, water in the middle layer, and a shrimp protein at the lower layer. The method provided by the invention adopts waste biomass obtained after shrimps are processed as a raw material, and can simultaneously extract four high-valued substances, thereby not only improving the utilization rate of raw materials, but also shortening the production cycle; and no organic solvent is added, so that the method is clean, green and environmentally friendly.
Description
technical field [0001] The invention relates to a method for extracting useful substances from a shrimp shell, in particular to a method for extracting astaxanthin, shrimp protein chitosan, calcium citrate and other useful substances from the shrimp shell by using a biological enzyme method. Background technique [0002] The chemical name of astaxanthin is 3.3'-dihydroxy-4.4'-diketone-B, B--carotene, pigment Aj067-69, CAS NO: 472-62-7, molecular formula is C 40 h 52 o 4 , molecular weight: 596.86. Pure astaxanthin is dark reddish brown, with a melting point of 224°C, insoluble in water, and soluble in most organic solvents. is a red pigment that imparts a pink color to aquarium fish, salmon, shrimp and flamingos. Astaxanthin has a super strong antioxidant capacity, and also has the functions of anti-tumor, anti-aging, enhancing the body's immunity, and preventing cardiovascular and cerebrovascular diseases. Therefore, astaxanthin has a very wide range of applications in...
Claims
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