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Preparation method of magnetic micro-beads for extracting nucleic acid

A technology of magnetic microbeads and nucleic acids, applied in DNA preparation, chemical instruments and methods, alkali metal oxides/hydroxides, etc., can solve the problems of small surface energy, large particle size of biological magnetic beads, low nucleic acid adsorption efficiency, etc. , to achieve the effect of small particle size, increased surface energy, and prolonged suspension time

Active Publication Date: 2016-06-08
HEILONGJIANG HEIKE TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention aims to solve the problems of large particle size, small surface energy and low nucleic acid adsorption efficiency in existing biological magnetic beads, and provides a preparation method of magnetic microbeads for nucleic acid extraction

Method used

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Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0048] Specific embodiment one: the preparation method of magnetic microbeads for nucleic acid extraction in this embodiment is carried out according to the following steps:

[0049] 1. Add nano-Fe to 0.5mol / L citric acid solution 3 o 4 , adjust the pH value of the solution to 5.5, stir under nitrogen protection, and then add nano-Fe 3 o 4 Take it out, wash it with acetone, and put the nano-Fe 3 o 4 Put it into deionized water for ultrasonic dispersion, then add absolute ethanol to form an ethanol-water system, and adjust the pH value of the solution to 6, and then add nano Fe 3 o 4 Add ethyl orthosilicate dropwise to the ethanol-water solution, raise the temperature to 60-80°C, stir and react for 8 hours, and finally add nano-Fe 3 o 4 Rinse with deionized water and absolute ethanol in turn, and dry in an oven to obtain Fe 3 o 4 / SiO 2 Magnetic microbeads; Nano Fe 3 o 4 The particle size is 13nm;

[0050] Two, the Fe prepared in step one 3 o 4 / SiO 2 Add the ma...

specific Embodiment approach 2

[0055] Specific embodiment two: the difference between this embodiment and specific embodiment one is: use NH in step one 3 ·H 2 O adjusts the pH. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0056] Embodiment 3: This embodiment is different from Embodiment 1 or 2 in that: in step 1, stir for 2 hours under the protection of nitrogen. Others are the same as in the first or second embodiment.

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Abstract

The invention provides a preparation method of magnetic micro-beads for extracting nucleic acid and relates to the preparation method of the magnetic micro-beads, aiming at solving the problems of existing biological magnetic beads that the grain diameter is large, the surface energy is small and the adsorption efficiency of the nucleic acid is low. The method 1 comprises the following steps: 1, preparing Fe3O4 / SiO2 magnetic micro-beads; 2, washing the magnetic micro-beads; 3, preparing magnetic micro-beads bonded with epoxy groups on the surfaces; 4, preparing magnetic micro-beads bonded with glycol groups on the surfaces. The method 2 comprises the following steps: 1, preparing the Fe3O4 / SiO2 magnetic micro-beads; 2, washing the magnetic micro-beads; 3, preparing the magnetic micro-beads bonded with the epoxy groups on the surfaces; 4, preparing magnetic micro-beads coated with iminodiacetic acid on the surfaces. The grain diameter of the magnetic beads prepared by the method provided by the invention is small and is nano-grade; the surface energy of the magnetic beads is improved and suspension time of the magnetic beads in an extracting solution is prolonged; the magnetic beads can be in more efficient contact with target extract, and the extracting efficiency of the target extract is improved. The preparation method is used for preparing the magnetic micro-beads for extracting the nucleic acid.

Description

technical field [0001] The invention relates to a preparation method of magnetic microbeads. Background technique [0002] With the continuous wide application of molecular biology technology, molecular biology technology has gradually changed from small-scale laboratory research in the past to large-scale programmed research such as disease diagnosis, genome sequencing, and epidemiological investigation of disease genes. practical means of operation. Taking human genome sequencing as an example, nucleic acid extraction, purification, and sequencing are required year after year. A lot of time of many excellent senior scientific researchers is spent in simple repetitive work, which is really a huge waste. At the same time, the inevitable irregularity of manual operation is likely to cause errors. Human genome sequencing generally needs to be repeated more than 3 times, which further increases the work intensity. [0003] Magnetic polymer microbeads, referred to as magnetic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/22B01J20/28B01J20/30C12N15/10
CPCB01J20/0229B01J20/103B01J20/22B01J20/28007B01J20/28009B01J20/28019C12N15/1013
Inventor 黄波李鹏谢洋杨帆付东隋新张晓臣
Owner HEILONGJIANG HEIKE TECH CO LTD
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