A method of preparing a grosvenor momordica fruit extract

Abstract

The invention discloses a method of preparing a grosvenor momordica fruit extract through continuous countercurrent extraction. The method includes directly feeding grosvenor momordica fruit into a continuous countercurrent extraction device after the grosvenor momordica fruit is crushed, extracting the grosvenor momordica fruit, and performing filtration to remove impurities, decoloring, adsorption purification, and concentration spray drying to obtain the extract. The content of mogroside V in the extract is not less than 40%. According to the method, only pure water or water-containing ethanol is adopted as a purification solvent in the whole process so that the method is safe and nontoxic. The method is simplified and capable of continuous operation so that a large amount of labor and resources can be saved. Preparing equipment and site requirements are low. The method is suitable for industrial large-scale production.

Description

technical field [0001] The invention belongs to the field of extracting, separating and preparing effective components of natural plants, and relates to a method for preparing a Luo Han Guo extract, in particular to a method for preparing a Luo Han Guo extract with a mogroside V content greater than 40%. Background technique [0002] Luo Han Guo is the fruit of the genus Cucurbitaceae. It is a multi-purpose medicinal and edible medicinal material. Luo Han Guo is extremely sweet, cool in nature, and non-toxic. It enters the spleen and lung meridian. It has the functions of clearing lung and relieving cough, moistening bowel and laxative, clearing heat and detoxification, benefiting liver and spleen, calming thirst, promoting gastrointestinal function, anti-virus, anti-tumor, Various pharmacological effects such as lowering blood sugar and blood pressure. A large amount of research data shows that Luo Han Guo not only contains a variety of pharmacologically active ingredients...

AI Technical Summary

Problems solved by technology

[0004] The research on the preparation of Luo Han Guo extract is mainly concentrated in China, and there are many related patent literature reports, but few of them can be actually applied to production, mainly reflected in the following aspects: (1) The process is cumbersome and the yield is low; (2) The use of a large amount of organic solvents requires high equipment and large investment; (3) the process consumes a lot of energy and costs; (4) the introduction of other substances in the process affects product quality

For example, CN101029071B discloses a method for preparing high-purity mogroside V from Luo Han Guo. The method is to extract the dried fruit of Luo Han Guo with alcohol, concentrate, extract with ethyl acetate and n-butanol, and then use silica gel column, C18 reverse phase silica gel column chromatography , high-purity sweet glycoside V product after drying, the main defects of this method are cumbersome process, large solvent loss, low yield, which is not conducive to large-scale industrial production; CN100572552C discloses Luo Han Guo extract with mogroside V ≥ 40% and its preparation The method is to extract the fresh fruit of Luo Han Guo with water, concentrate, enzymolyze, absorb it with a macroporous resin, decolorize it with an ion exchange resin, and finally concentrate and dry it to obtain the product. Because this method does not filter before loading the column, the subsequent processing cost of the resin is Larger, unfavorable for continuous production; CN101407535B discloses a preparation method of high-purity mogroside V, which uses a certain purity of Luo Han Guo extract as raw material, dissolves in methanol, extracts with acetone, absorbs on normal phase silica gel, and separates , crystallization and recrystallization to obtain the final product. This method has a simple process, but a large amount of organic solvent is used in the process, which has higher requirements on the production site. In addition, the obtained product also has a certain amount of organic solvent residue, which affects the quality; CN101863946B discloses a A method for extracting high-content mogroside V from dried grosvenor fruit. The method is to crush the dried grosvenor fruit, carry out alcohol extraction, concentration, centrifugation, macroporous resin adsorption separation, ion exchange resin decolorization, and finally concentration and drying to obtain a finished product. Because this method is not filtered, there are more macromolecular impurities in the upper column liquid, which affects the adsorption performance of the resin, the utilization rate is low, the follow-up treatment is relatively difficult, and the product cost is high.