Method for extracting high active immunoglobulin in blood

A technology of immunoglobulin and extraction method, which is applied in the field of extraction of highly active immunoglobulin in blood, can solve the problems of cumbersome extraction steps, low immunoglobulin content, and long time, so as to simplify production steps, simplify extraction steps, reduce pollution effect

Inactive Publication Date: 2005-01-12
JINDADI BIOENG ZHEJIANG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The salting-out method can be produced at normal temperature, but it needs to go through a long period of desalination; most of the organic solvent precipitation methods need to be reacted at low temperature, and the extraction steps are cumbersome; although the ultrafiltration method can remove some impurities, it can have a high yield. The yield of immunoglobulin, but the content of immunoglobulin is relatively low

Method used

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  • Method for extracting high active immunoglobulin in blood

Examples

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example 1

[0013] Get 500g blood, at ethanol concentration 8% (mass ratio), precipitation removes fibrin under pH7.2; Then adjust ethanol concentration to 25% (mass ratio); Add distilled water to 500g, stir and dissolve, and ultrafilter three times by dilution ultrafiltration to obtain 150g of ultrafiltrate; obtain 9.2g of high-activity immunoglobulin powder by freeze-drying. The supernatant after extracting the immunoglobulin was vacuum distilled at 45° C. and 0.1 Mpa to obtain 300 g of a recovered solution containing 72.5% ethanol. The remaining solution of distillation obtains protein powder 28.6g (mainly albumin) through spray-drying, can make the content of protein and be the feed powder of the high protein of 69.2%.

example 2

[0015] Get 500g of blood, at a concentration of 12% acetone (mass ratio), precipitate and remove fibrin at pH7; then adjust the acetone concentration to 28% (mass ratio); precipitate immunoglobulin at pH5.6, in crude immunoglobulin Add distilled water to 500g, stir to dissolve, and ultrafilter three times by dilution ultrafiltration to obtain 150g of ultrafiltrate; obtain 10.1g of immunoglobulin powder by freeze-drying. The supernatant after the immunoglobulin extraction was vacuum distilled at 48° C. under 0.1 Mpa to obtain 300 g of a recovered solution containing 72.5% acetone. Re-distilling the remaining solution to obtain protein powder 27.6g (mainly albumin) through spray-drying, can be made into a high-protein feed powder with a protein content of 68.3%.

example 3

[0017] Take 500g of blood, and remove fibrin by precipitation at pH 7.5 at a polyethylene glycol concentration of 15% (mass ratio); then adjust the polyethylene glycol concentration to 35% (mass ratio); precipitate immunoglobulin at pH 6.3 , add distilled water to the crude immunoglobulin to 500g, stir and dissolve, and ultrafilter three times by dilution ultrafiltration to obtain 150g of ultrafiltrate; obtain 8.9g of immunoglobulin powder by freeze-drying. The supernatant after the immunoglobulin extraction was vacuum distilled at 50° C. and 0.1 MPa to obtain 300 g of a recovered solution containing 72.5% polyethylene glycol. Re-distilling the remaining solution to obtain protein powder 28.9g (mainly albumin) through spray drying, which can be made into a high-protein feed powder with a protein content of 69.8%.

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Abstract

The invention discloses a method for extracting high-activity immunoglobulin in blood, belonging to the range of food and feed additive and bio-chemical detection reagent preparation technology. Said invention is aimed at overcoming the defect of the existent technique and provides the method adopting low-temp. organic solvent and combining it with ultrafiltration process. Said method includes the following steps: firstly, using low-temp. organic solvent to extract immunoglobulin in the blood, then ultrafiltering and purifying immunoglobulin, finally freze-drying or low-temp. spray-drying so as to obtain the invented product. Its immunoglobulin content can be up to above 65%. Said invention can simplify production steps of low-temp. ethyl alcohol precipitation method, can reduce denaturing rate of immunoglobulin, and can recover albumin and ethyl alcohol and can fully recover and utilize wastes.

Description

technical field [0001] The invention belongs to the technical scope of preparing food, feed additives and biochemical detection reagents, and in particular relates to a method for extracting highly active immunoglobulins in blood. Background technique [0002] Immunoglobulin (Ig) is a globulin that has antibody activity and can specifically bind to the corresponding antigen. It is produced by lymphocytes in the immune response to antigen stimulation in vertebrates and is commonly found in mammals. A type of protein in animal blood, interstitial fluid, lymph fluid, and in vitro secretion fluid. They have important immune and physiological regulation functions in animals, and play an important role in improving the health of infants, middle-aged and elderly people, and people with low immunity. It is widely used in the development of new functional food additives, livestock feed, biochemical diagnosis, detection reagents, new biological drugs and medicine, etc. [0003] The ...

Claims

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Application Information

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IPC IPC(8): A23K20/147A23L33/17C07K1/34C07K16/18
Inventor 陈伯宜罗永康董翠霞周富宽金东灿
Owner JINDADI BIOENG ZHEJIANG
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