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Method for extracting rice DNA (Deoxyribonucleic Acid)

A rice extraction technology, applied in the direction of DNA preparation, recombinant DNA technology, etc., can solve the problems that are not suitable for the rapid preparation of a large number of DNA samples, and achieve the effect of simplifying the extraction steps

Inactive Publication Date: 2013-03-20
SHANGHAI AGROBIOLOGICAL GENE CENT
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Problems solved by technology

[0003] In order to solve the defect that the existing technology is not suitable for the rapid preparation of a large number of DNA samples, the present invention provides a fast, simple and high-throughput method for extracting rice DNA

Method used

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  • Method for extracting rice DNA (Deoxyribonucleic Acid)

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Embodiment Construction

[0022] SSR (Simple Sequence Repeats) marking is a molecular marking technology based on specific primer PCR developed in recent years. ~6) is a tandem repeat sequence of dozens of nucleotides consisting of repeating units. Since the sequences on both sides of each SSR are generally relatively conserved single-copy sequences.

[0023] Use the molecular marker RM520 to detect the progeny populations of Hanhui 3 and B5 materials, use a 96-well deep-well plate for sampling (field materials are selected to be planted into 8 plants×12 rows), and the sampling direction and field number of the material in the deep-well plate Make a note.

[0024] Take leaves about 5cm rolled and put them into a centrifuge tube in a 1.1ml deep-well plate. Add 1 small steel ball into the well, freeze in liquid nitrogen for 2-3 minutes, install a 96-deep well plate adapter and symmetrically install it in a mixer mill (Mixer Mill Type, Model: MM301), and then grind it at 25- Oscillate at a frequency of ...

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Abstract

The invention discloses a method for extracting DNA (Deoxyribonucleic Acid) of a rice leaf blade or a rice seed rapidly and efficiently by using a 96 deep-hole plate and a mixer mill, relating to the field of biology. The method is a rapid and effective plant DNA extraction method, the whole extraction course is free from single-shotgun operation but undergoes stranded-shotgun operation completely, and in addition, serial numbers do not need to be written, so that a rapid high-flux extraction method is realized. The method can be applied to rice molecular breeding, purity identification, seed authenticity detection, gene cloning and the like.

Description

technical field [0001] The invention relates to a method for extracting DNA, in particular to a method for extracting DNA from plant species such as rice leaves and grains. Background technique [0002] SSR markers have the characteristics of rapidity, accuracy, high information content, co-dominant inheritance, easy analysis and reliable repetition. Therefore, since the technology was produced in 1989, it has been widely used in rice gene mapping and QTL analysis, rice variety DNA fingerprinting, population genetic diversity analysis, molecular marker-assisted breeding, hybrid combination authenticity and purity identification, transgenic varieties detection etc. DNA extraction is a prerequisite for PCR analysis. At present, the process of DNA extraction using rice leaves or seeds as research materials mainly uses 2ml centrifuge tubes, and grinds, centrifuges, transfers tubes, and adds samples with a single gun in each single tube. There are many extraction steps and a l...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/10
Inventor 刘国兰刘毅王飞名王加红张安宁余新桥罗利军
Owner SHANGHAI AGROBIOLOGICAL GENE CENT
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