Extraction separation preparation method of lignin monomers in schisandra chinensis

A technology of schisandra and lignans, applied in the field of natural medicinal chemistry

Active Publication Date: 2014-04-02
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But so far there is no report on the separation and purification of lignan monomers in Schisandra chinensis by supercritical extraction and high-speed countercurrent chromatography

Method used

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  • Extraction separation preparation method of lignin monomers in schisandra chinensis
  • Extraction separation preparation method of lignin monomers in schisandra chinensis
  • Extraction separation preparation method of lignin monomers in schisandra chinensis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Embodiment 1: supercritical fluid extraction

[0048] Take the fruit of Schisandra chinensis and crush it to get Schisandra medicinal material powder with a particle size of 50-65 mesh. Take 2 g of Schisandra medicinal material powder and put it into a supercritical fluid extraction tank. The extraction temperature is 40 °C, the extraction pressure is 25 MPa, CO 2 The flow rate is 2L / min, the extraction time is 1h, and the crude extract of Schisandra chinensis is obtained. Taking Schisandrin A as a representative, the calculated extraction rate is 0.5294%.

Embodiment 2

[0049] Embodiment 2: supercritical fluid extraction

[0050] Take the fruit of Schisandra chinensis and crush it to obtain the powder of Schisandra chinensis medicinal material with a particle size of 10-20 meshes. Take 3g of the medicinal material powder of Schisandra chinensis and put it into a supercritical fluid extraction tank. The extraction temperature is 35°C, the extraction pressure is 14MPa, CO 2 The flow rate is 2L / min, the extraction time is 2h, and the crude extract of Schisandra chinensis is obtained. Taking Schisandrin A as a representative, the calculated extraction rate is 0.3926%.

Embodiment 3

[0051] Embodiment 3: supercritical fluid extraction

[0052] Take the fruit of Schisandra chinensis and pulverize it to obtain the medicinal material powder of Schisandra chinensis. The particle size is 50-65 meshes. Take 3g of the medicinal material powder of Schisandra chinensis and put it into a supercritical fluid extraction tank. The extraction temperature is 35°C, the extraction pressure is 30MPa, CO 2 The flow rate is 2L / min, the extraction time is 2h, and the crude extract of Schisandra chinensis is obtained. Taking Schisandrin A as a representative, the calculated extraction rate is 0.6001%.

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Abstract

The invention discloses an extraction separation preparation method of lignin monomers in schisandra chinensis, which comprises the following steps: performing CO2 supercritical extraction of schisandra chinensis medicinal material powder, performing separation of crude extract by high-speed countercurrent chromatography, performing gradient elution, wherein the high-speed countercurrent solvent system A comprises n-hexane, ethyl acetate, methanol and water, fully mixing, standing, performing elution with the upper phase A as a stationary phase A and the bottom phase A as a mobile phase A to obtain schisandrol A and schisandrol B, changing the solvent system, increasing the volume ratio of methanol to water, performing elution with the bottom phase B as a mobile phase B to obtain schisantherin B; changing the solvent system, further increasing the volume ratio of methanol to water, performing elution with the bottom phase C as a mobile phase C to obtain schisandrin B. According to the invention, the method combines supercritical fluid extraction with high-speed countercurrent chromatography separation; the product can be used directly for subsequent monomer separation and purification without complicated sample treatment after extraction; 5 lignin monomers can be obtained by only separation once; the method is simple and high-efficient.

Description

technical field [0001] The invention belongs to the field of natural medicinal chemistry and relates to a fast and efficient combined preparation method for extracting, separating and purifying lignan monomer components from Schisandra chinensis. It specifically relates to the extraction and separation of monomer components in Schisandra chinensis by means of supercritical extraction combined with high-speed countercurrent chromatography. Background technique [0002] Schisandra chinensis is the mature fruit of Schisandra chinensis (Turcz.) Baill or Schisandra sphenanthera Rehd.et Wils. The former is commonly called "Northern Schisandra", and the latter is commonly called "Southern Schisandra". It was first recorded in "Shen Nong's Materia Medica" and listed as top grade. Schisandra is warm in nature, sweet and sour in taste, and returns to the lung, heart and kidney channels. It has the effects of astringent and astringent, nourishing qi and promoting body fluid, invigor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D317/70C07C43/21C07C43/23C07C41/34
CPCC07C41/34C07D317/70C07C43/21C07C43/23Y02P20/54
Inventor 楚楚章诗迪颜继忠童胜强李行诺
Owner ZHEJIANG UNIV OF TECH
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