57 results about "Schisandrin B" patented technology

Methods of application of Schisandrin B in the preparation of anticancer medications, and particularly for the preparation of medications for the treatment of multidrug resistant (MDR) cancer. The compound of Schisandrin B effectively reverses MDR cancer in combination with other anticancer chemotherapeutic agents. Schisandrin B reverses MDR cancer by inhibiting the drug efflux activity of P-glycoprotein, indicating its significance in clinical applications. Although it is of low toxicity, Schisandrin B is cytotoxic to human cancer cells, revealing its application in cancer chemotherapy. It is emphasized that this abstract is provided to comply with the rules requiring an abstract that will allow a searcher or other reader to quickly ascertain the subject matter of the technical disclosure. It is submitted with the understanding that it will not be used to interpret or limit the scope or meaning of the claims.

The invention relates to a schisandra fruit fingerprint spectrum quality control method, comprising that (1), adding 0.5g schisandra fruit to extract via microwave for 50min, filtering, (2), washing flow phase gradient that the spectrum column is Eclipse, as 0-40min, 60-20% A, 40-80% B, flow speed is 0.8-1.2ml/min, the wavelength is 210-280nm, the column temperature is 20-40Deg. C, and the sample amount is 10-20ul, (3), building standard fingerprint spectrum that the first peak is schisandra fruit alcohol 1, the third peak is schisandra fruit alcohol 2, the fourth peak is gemixin N/ isomer, the sixth peak is schisandra fruit I element, the seventh and eighth peaks are gemixin N/ isomer, the ninth peak is schisandra fruit II element, (4), controlling the quality of fingerprint spectrum that the check peak relative holding times are 1.00, 1.25, 1.63, 1.88, 3.18 and 3.27, (5), the schisandra fruit planting collecting method. The invention can control the quality of materials to assure the stable quality of product.

The invention discloses a method for extracting, separating and preparing lignin monomers from schisandra chinensis. The method comprises the following steps: performing supercritical extraction on schisandra chinensis medicinal powder by using CO2 to obtain a crude extract; performing primary high-speed counter-current chromatographic separation on the crude extract; forming a solvent system A by using hexane, ethyl acetate, methyl alcohol and water in the volume ratio of (2-10):(0-10):(0-10):5, wherein the upper phase A is a stationary phase A and the lower phase A is a mobile phase A; preparing a first schizandrol, a second schizandrol and a third schizandrol; recovering the separated stationary A to serve as a sample to be tested; performing secondary high-speed counter-current chromatographic separation; forming a solvent system B by using hexane, ethyl acetate, methyl alcohol and water in the volume ratio of (1-10):(0-4):(0-9):1, wherein the upper phase B is a stationary phase B, and the lower phase B is a mobile phase B; preparing deoxyschizandrin and schisandrin b. By adopting the method which integrates supercritical extraction and high-speed counter-current chromatographic separation, the deoxyschizandrin and the schisandrin b can be directly used for separating and purifying subsequent monomers without complex sample treatment after the extraction; the steps of extracting and separating are simple and efficient.

The invention discloses a method for extracting deoxyschisandrin and schisandrin B from schisandra, comprising the following steps: 1) crushing the schisandra, extracting with 80-95% ethanol by volume concentration, concentrating extracting solution to obtain concentrated solution; 2) adding ethanol in the concentrated solution and ensuring that the concentration of ethanol is 60-65% by weight, centrifuging and collecting supernate; 3) passing the supernate through a macroporous resin column, eluting with 55-65% ethanol by volume concentration, wherein the weight of ethanol is 1.5-2.5 times of that of the resin, collecting eluent A, and drying to obtain deoxyschisandrin extract; and 4) eluting with 85-95 % ethanol by volume concentration, wherein the weight of ethanol is 2.5-3.5 times of that of the resin, collecting eluent B, and drying to obtain schisandrin B extract. By using the method in the invention, the deoxyschisandrin and schisandrin B are eluted in turn, so that the deoxyschisandrin and schisandrin B are separated, wherein the content of the deoxyschisandrin is 0.8-1.2%, and the content of the schisandrin B is 3-4%.

The invention relates to a quality detection method for a liver protection dropping pill of traditional Chinese medicine preparation. The quality detection method comprises the following steps: measuring the content of effective components of the liver protection dropping pill, including saikoside a and schizandrin, and identifying schisandra chinensis, pulvis fellis suis and artemisia capillaris in the liver protection dropping pill. The quality detection method also comprises the following detection steps: (1) with the saikoside a as a reference substance, measuring whether a radix bupleuri component is contained in a liver protection dropping pill recipe by adopting a high efficiency liquid chromatography method; (2) with the schizandrin as the reference substance, measuring whether a schizandrin component is contained in the liver protection dropping pill by adopting the high efficiency liquid chromatography method; (3) with the schisandrin b as the reference substance, identifying whether a schisandra chinensis component is contained in the liver protection dropping pill by adopting a thin-layer chromatography method; (4) with hyodeoxycholic acid as the reference substance, identifying whether a pulvis fellis suis component is contained in the liver protection dropping pill by adopting the thin-layer chromatography method; and (5) with chlorogenic acid as the reference substance, identifying whether an artemisia capillaris component is contained in the liver protection dropping pill by adopting the thin-layer chromatography method. The quality detection method disclosed by the invention can be used for effectively and reliably controlling the quality of the liver protection dropping pill, and the method is scientific, feasible and reliable.

The invention discloses a composition for protecting lever and achieving a sobering function as well as a preparation method and application of the composition. The composition contains 30-180 parts by weight of sesamin, 1-6 parts by weight of schisandrin B, 150-900 parts by weight of semen hoveniae extract, 150-900 parts by weight of pueraria lobata extract, 0.0012-0.0072 parts by weight of vitamin B12 and 14-84 parts by weight of vitamin E.

The invention provides a method for extracting Schisandrin B from Schisandra chinensis. The method adopts macroporous adsorption resin column chromatography, silica gel column chromatography and recrystallization to extract and separate Schisandrin B from the Schisandra chinensis medicine in order to obtain high-purity Schisandrin B, and accords with the technological characteristics of industrial production. The method has the following advantages: the purity of the prepared Schisandrin B can reach 90-100%, and the method can be applied to the industrial large-scale preparation of the Schisandrin B.

The present invention provides a preparation for treatment or prevention of a condition in a patient, said preparation comprising Schisandrin B. The concentration of Schisandrin B in the preparation may be between about 0.01 and about 0.1%, or it may be between about 20 and about 40% w/w or w/v. The preparation may additionally comprise one or more components selected from the group consisting of herbal extracts, fluids, solvents, antioxidants, preservatives, electrolytes, salts and pH control agents.

The invention discloses a medical composite for preventing and curing Alzheimer's disease (AD). The composite comprises the following raw materials in parts by weight: 10-40 parts of hyperin, 20-80 parts of icariin and 5-20 parts of Schisandrin B. Experiments of rat place navigation and space exploration prove that the medical composite of the invention can increase the ability of spatial learning and memory of AD rat model, namely reduce the escape latency and scouting distance and obviously increase the percent of scouting time in quadrant of original platform to total time and the percent of scouting distance in quadrant of original platform to total distance in space exploration experiments. Therefore, the extract composite can be used for preventing and curing AD.

The invention discloses an extraction separation preparation method of lignin monomers in schisandra chinensis, which comprises the following steps: performing CO2 supercritical extraction of schisandra chinensis medicinal material powder, performing separation of crude extract by high-speed countercurrent chromatography, performing gradient elution, wherein the high-speed countercurrent solvent system A comprises n-hexane, ethyl acetate, methanol and water, fully mixing, standing, performing elution with the upper phase A as a stationary phase A and the bottom phase A as a mobile phase A to obtain schisandrol A and schisandrol B, changing the solvent system, increasing the volume ratio of methanol to water, performing elution with the bottom phase B as a mobile phase B to obtain schisantherin B; changing the solvent system, further increasing the volume ratio of methanol to water, performing elution with the bottom phase C as a mobile phase C to obtain schisandrin B. According to the invention, the method combines supercritical fluid extraction with high-speed countercurrent chromatography separation; the product can be used directly for subsequent monomer separation and purification without complicated sample treatment after extraction; 5 lignin monomers can be obtained by only separation once; the method is simple and high-efficient.

The invention belongs to the field of traditional Chinese medicines or the pharmaceutical chemistry, and relates to a medicinal composition for resisting hepatic fibrosis and/or hepatic cirrhosis. The medicinal composition includes ursolic acid and schisandrin B. The invention also relates to a use of the medicinal composition in the preparation of medicines for treating and/or preventing and/or adjunctively treating hepatic fibrosis and hepatic cirrhosis, or medicines for inhibiting hepatic stellate cell proliferation or inhibiting hepatic stellate cell DNA synthesis, and a method for inhibiting hepatic stellate cell proliferation or inhibiting hepatic stellate cell DNA synthesis. The medicinal composition can obviously improve the hepatic fibrosis degree, has the advantages of few components, strong effect, simple preparation, no obvious hepatotoxicity, and good market prospect, and is of certain practical significance for promoting traditional Chinese medicinal modernization.

The invention provides an application of schisandrin b in preparing an NADPH oxidase inhibitor. The NADPH oxidase comprises various sub-types of oxidase. In in-vitro enzymatic experiments, the schisandrin b is capable of inhibiting super-oxygen ion generation reaction catalyzed by NADPH oxidase and reactive oxygen generation related to NADPH oxidase in cells, and does not have the function of scavenging free radicals, thus being an inhibitor of the NADPH oxidase. Furthermore, the NADPH oxidase is capable of changing the level of a plurality of signal transduction pathways and inhibiting generation of tumor metastasis, and can be applied to preparation of medicaments for treating tumor metastasis.

The invention belongs to the technical field of the detection of components of traditional Chinese medicines, and discloses a detection method of effective components of radix seu caulis schisandrae propinquae and application. The detection method comprises steps of preparation of standard substance solutions of rutin, schizandrin A and schisandrin B respectively, preparation of a test sample solution, HPLC (High Performance Liquid Chromatography) detection and the like, wherein chromatographic conditions of the HPLC detection are as follows: a methanol-1 percent glacial acetic acid solution is used as mobile phase eluent; the gradient elution is carried out for 90min; volume fractions of methanol in the eluent corresponding to each time interval of the elution are respectively: 20 percentduring 0min to 20min, 40 percent during 20min to 40min, 60 percent during 40min to 60min, 80 percent during 60min to 80min and 100 percent during 80min to 90min; a flow speed is 1mL/min; a detectionwavelength is 257nm. The detection method uses the HPLC detection with the gradient elution, can be used for measuring three functional effective components in the radix seu caulis schisandrae propinquae at the same time, and can be used for more completely reflecting a pharmacodynamic effect of the radix seu caulis schisandrae propinquae.

The invention relates to an application of schisandrin B in the preparation of a diluted liquid for preserving boar semen. The invention also relates to a diluted liquid for preserving boar semen. The diluted liquid comprises glucose, sodium bicarbonate, sodium citrate, EDTA, critic acid, potassium chloride, gentamycin, and schisandrin B. The effect of the provided diluted liquid for preserving boar semen is good and reliable. The high quality semen stored at a room temperature can be provided for pig artificial insemination. After the boar semen is preserved in the diluted liquid at a temperature lower than 17 DEG C for five days, the semen activity is still above 0.6.

The invention relates to a combined type method for extracting schisandrin b from fructus schisandrae. The method comprises the steps of pretreatment of raw materials, preparation of compound extractant, extraction and separation and purification; the compound extractant formed by appropriate ionic liquid, assistant and hexadecyl trimethyl ammonium bromide is adopted, solid sodium carbonate is added for auxiliary extraction, and besides, microwaves and expanded solvent are adopted for complementary extraction, therefore, the method for extracting the schisandrin b from the fructus schisandrae with high yield is achieved finally, the material utilization rage is improved, waste discharge and environment pollution, caused by a great amount of organic solvents, are avoided, and wide industrial application prospect is achieved.

The invention provides a method for simultaneously testing contents of seven lignans in schisandra chinensis. According to the method, a chromatographic column ZORBAX Eclipse Plus C18 column (1.8 [mu]m, 100 mm*2.1 mm) is used; a mobile phase, namely acetonitrile-water, is adopted for gradient elution; the flowing speed is 0.3 mL*minute-1; the column temperature is 40 DEG C; the detection wavelength is 220 nm; and the sample feeding volumn is 1 [mu]L. Results show that components, namely schisandrol A, schisandrol B, schisantherin A, schisantherin B, schizandrin A, schisandrin B and schisandrinC, generally meet linear separation and are in good linear relationship, the average sampling recycling rate is within 98.29-102.5%, and the RSD (Relative Standard Deviation) is within 1.4-1.9%. Testresults show that the method is rapid, stable and reliable and applicable to testing on contents of lignans in southern and northern schisandra chinensis in different production areas, and identification on southern and northern schisandra chinensis.

Use of Fructus schisandrae in preparation of medicaments for preventing and reducing toxicity and side effects of antineoplastic agents. The toxicity and side effects of antineoplastic agents are cardiovascular toxicity, hepatotoxicity, nephrotoxicity, suppression of bone marrow, immunosuppression, or alopecia etc induced by antineoplastic agents. Fructus schisandrae and extracts thereof, especially ethanol extracts, schisandrin B, are effective in reducing antineoplastic agent's toxicity and side effects.

The invention relates to a traditional Chinese medicine formula for treating lung and kidney qi deficiency syndrome of chronic obstructive pulmonary disease (COPD). The problem of the medicine for treating the lung and kidney qi deficiency patient in the stable period of COPD is effectively solved. The traditional Chinese medicine formula comprises 6-20mg ginseng extract, 10-100mg icariin, 1-8mg paeonol, 0.5-6mg nobiletin, 5-20mg paeoniflorin, 3-12mg schisandrin b, 1-8mg peimine, 5-20mg hesperidin, 3-12mg astragalus polysaccharide and 1-8mg astragaloside. The traditional Chinese medicine formula provided by the invention can be effectively applied to the treatment for the lung and kidney qi deficiency syndrome of chronic obstructive pulmonary disease and the preparation of the preparationthereof. Repeated test proves that the traditional Chinese medicine formula is capable of improving COPD lung function, relieving lung tissue pathological injury and inflammatory cell infiltration andimproving inflammatory response and oxidative stress and can be used as long-term used medicine in the stable period for treating COPD. The traditional Chinese medicine formula has the advantages ofreasonable composition, scientific method, high innovation, accurate curative effect and obvious economic and social benefits.

The present invention relates to a discrimination method, particularly to an agricultural product production place identification method, which is mainly used for schisandra chinensis production place identification. According to the agricultural product production place identification method, the independent variable factors in the crushed schisandra chinensis sample is qualitatively and/or quantitatively determined, and is used to carry out model regression, and the belonging production place of the sample is determined according to the dependent variable level, wherein the independent variable factors (Xj) are [delta]<15>N, the schisandrin content, the schisantherin A content, the schizandrin A content, the schisandrin B content, the B content and the Sr content. According to the present invention, the schisandra chinensis is adopted as the demonstration to develop the production place traceability research to screen the geographic labels for effectively indicating the schisandra chinensis production place source, such that the drug food safety of the consumer can be easily ensured, the original production place protection of the authentic herbs can be achieved, and the theoretical basis is provided for the optimized planting of the schisandra chinensis. In addition, the technology can further be promoted and applied for the agricultural product quality safety and control, and has broad application prospects.

The invention discloses a method for carrying out simultaneous determination on contents of schisandrin, schisantherin, deoxyschizandrin and schisandrin B in a Chinese magnoliavine raw material or a Chinese magnoliavine extract. The method employs means of crushing, sieving, methanol ultrasonic extraction, calibrating volume and filtering to treat schisandra chinensis or a Chinese magnoliavine extract to obtain a sample solution for testing; a high performance liquid chromatography is utilized for simultaneous determination on contents of four lignan components. The method utilizes a reverse direction distribution chromatographic theory to carry out analysis on the sample by gradient elution, under conditions of a column temperature of 25-45 DEG C, mobile phases of methanol and 0.05-0.2% trifluoroacetic acid aqueous solution (methanol and trifluoroacetic acid aqueous solution in a volume ratio of 50:50-100:0), an elution time of 30-60 min, a flow velocity of 0.7-1.5 ml / min and a detection wavelength of 210-280 nm. The quality detection method is simple, rapid, sensitive and accurate.

The invention discloses a Chinese magnoliavine active extract, which comprises schisandrin, deoxyschizandrin, and schisandrin B. The preparation method comprises reflux extraction, concentration and water precipitation, adsorption, elution, and drying. Ethanol (85-95%) is used to extract the Chinese magnoliavine active substances through reflux extraction; then the active substances are purified by water precipitation, adsorption, macroporous resin elution, and reversed-phase high-performance liquid chromatography to obtain schisandrin, deoxyschizandrin, and schisandrin B, and the product quality is high. The production technology is simple, each operation has accurate data, the repeatability is good, the product quality is stable, ethanol is low in toxicity, and the extracted Chinese magnoliavine active substances are very safe and green.

The invention discloses a method for inducing human placenta mesenchymal stem cells to differentiate into hepatocytes in vitro. Schisandrin B is added in a hepatocellular-like induction stage, the effect of inducing the human placenta mesenchymal stem cells to differentiate into the hepatocytes with functions can be effectively improved, the used stem cells are the human placenta mesenchymal stem cells, and are rich in source, convenient to extract, clear in induction composition component and simple in induction operation, and the method has industrialization and commercialization prospects.

The invention discloses a preparation process for a traditional Chinese medicine extract and particularly relates to a preparation process for a schisandrol extract. The preparation process for the schisandrol extract comprises the following steps of pretreatment, extraction, concentration, reflux extraction, ethanol precipitation, reconcentration and drying. The schisandrol extract prepared by the preparation process has a medicinal function of lowering serum glutamic pyruvic transaminase and is used for treating chronic hepatitis. Verified by experiments, schisandrin b and total lignans only can be fully extracted in 85 percent of alcohol. When the extraction is performed by adopting the alcohol with other concentrations, active constituents cannot be completely extracted, and then, the curative effect is influenced. Meanwhile, by adopting the ethanol precipitation process in the preparation process disclosed by the invention, impurities, such as grease, can be removed, so that the purity is greatly increased, the quality is higher, and the curative effect is comparatively obvious.