Method for simultaneously testing contents of seven lignans in schisandra chinensis

A technology of schisandra and lignan, applied in the field of medicine, can solve the problems of long time, high consumption of organic solvents, etc.

Inactive Publication Date: 2018-06-29
HUAZHONG UNIV OF SCI & TECH
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Problems solved by technology

At present, the liquid phase method for the determination of lignans in Schisandra chinensis generally adopts the HPLC method [see: 1. Wei H, Sun L, Tai Z, et al. A simple and sensitive HPLC method for the simultaneous determination of eight bioactive components and fingerprint analysis of Schisandra sphenanthera[J].Analytica Chimica Acta,2010,662(1):97-104; 2. Wang Tianjiao, Zhang Yuhang, Zhang Tielun. Study on the effect of determining the content of six lignin components in Schisandra granules by HPLC[J] ].Contemporary Medicine, 2015(22):37-38.] However, this method has the disadvantages of long time consumption and high consumption of organic solvents.

Method used

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  • Method for simultaneously testing contents of seven lignans in schisandra chinensis
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  • Method for simultaneously testing contents of seven lignans in schisandra chinensis

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Embodiment 1

[0057] 1 material

[0058] 1.1 Main instruments Agilent 1290 ultra-high performance liquid chromatography (including Agilent chromatographic workstation, G4226A high-performance autosampler and G4212A DAD detector (Agilent, USA); BP-211D 1 / 100,000 electronic balance (Sai Doris Scientific Instrument (Beijing) Co., Ltd., Germany), A12.04 millionth electronic balance (Sartorius Scientific Instrument (Beijing) Co., Ltd., Germany); TDL80-2B desktop centrifuge (Shanghai Anting Scientific Instrument Factory); Wait.

[0059] 1.2 The main reagents Schisandra Alcohol B, Schisandra Ester A, Schisandra Ester B, Schisandrin B, and Schisandra C reference substances were purchased from Chengdu Purifa Technology Development Co., Ltd. ); Schisandrin A and Schisandrin A reference substances were purchased from China National Institutes for Food and Drug Control (batch numbers were: JBSG-FAKJ, 1A11-1B9N); acetonitrile (Merck & Co., Ltd., Germany) was chromatographically pure, water Ultrapure w...

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Abstract

The invention provides a method for simultaneously testing contents of seven lignans in schisandra chinensis. According to the method, a chromatographic column ZORBAX Eclipse Plus C18 column (1.8 [mu]m, 100 mm*2.1 mm) is used; a mobile phase, namely acetonitrile-water, is adopted for gradient elution; the flowing speed is 0.3 mL*minute-1; the column temperature is 40 DEG C; the detection wavelength is 220 nm; and the sample feeding volumn is 1 [mu]L. Results show that components, namely schisandrol A, schisandrol B, schisantherin A, schisantherin B, schizandrin A, schisandrin B and schisandrinC, generally meet linear separation and are in good linear relationship, the average sampling recycling rate is within 98.29-102.5%, and the RSD (Relative Standard Deviation) is within 1.4-1.9%. Testresults show that the method is rapid, stable and reliable and applicable to testing on contents of lignans in southern and northern schisandra chinensis in different production areas, and identification on southern and northern schisandra chinensis.

Description

technical field [0001] The invention belongs to the field of medicine, and relates to a detection technology for active components of traditional Chinese medicinal materials, in particular to a method for measuring the content of seven kinds of lignans in Schisandra chinensis and an application thereof. Background technique [0002] The 2015 edition of the "Chinese Pharmacopoeia" stipulates that Schisandra chinensis (Turcz.) Baill. is the dried and mature fruit of the plant Schisandra chinensis (Turcz.) Baill. The main distribution area extends from Northeast Asia (Russian Far East, North Korea, Korea, Japan) to Northeast my country, North China, and the northern slope of the Qinling Mountains. .2003]. Schisandra sphenanthera is the dry mature fruit of Schisandra sphenanthera Rehd.et Wils. [1] , mainly distributed in Shaanxi, Shanxi, Gansu, Jiangxi, Hunan, Sichuan, Yunnan and other places [See: Chinese Academy of Sciences Editorial Committee of Flora of China. Flora of China...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 尹春萍马元春
Owner HUAZHONG UNIV OF SCI & TECH
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