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Application of Schisandrin-B in preparing medicine for treating tumor

A technology of schisandrin and tumor drugs, which is applied in the direction of anti-tumor drugs, drug combinations, and pharmaceutical formulas, and achieves good application prospects

Inactive Publication Date: 2005-06-01
高陈勇
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Wang Jianhua, Li Ligeng, Li En "Effects of Schisandrin B on the Proliferation and Differentiation of Rat Osteoblasts" report: Schisandrin B can promote the proliferation and differentiation of osteoblasts in vitro; (Research and Development of Natural Products, 2003, No. 5, 446), but there are no literature reports and patent publications to show that Schizandrin B has any application prospects in the preparation of tumor drugs

Method used

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  • Application of Schisandrin-B in preparing medicine for treating tumor
  • Application of Schisandrin-B in preparing medicine for treating tumor
  • Application of Schisandrin-B in preparing medicine for treating tumor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1: P-glycoprotein (P-gp) detection of multidrug resistant cells

[0045] (1) Experimental materials:

[0046] Cell lines: K562 / ADR, / K562 / VCR multi-drug resistant cell lines are high expression of P-gp protein as the main drug resistance mechanism, induced by Zhejiang University Cancer Institute, KBV200 multi-drug resistant cell lines are P- The high expression of gp protein is the main mechanism of drug resistance, which was purchased from the Institute of Hematology, Chinese Academy of Medical Sciences.

[0047] Reagent: The monoclonal fluorescently labeled antibody of P-gp is R-PE-17F9 from BD Company, USA.

[0048] Instruments: culture flasks, culture plates, carbon dioxide incubators, FCM.

[0049] (2) Experimental method:

[0050] Determination of cell P-gp expression: Take the cells of each strain in the logarithmic growth phase, collect the cells and make 1×10 6 / ml of the suspension, add primary antibody (1:400), react in the dark at 4°C for 45min, ...

Embodiment 2

[0054] Example 2: Reversal of drug resistance of Schizandrin B

[0055] (1) Experimental materials:

[0056] Cell lines: K562 / ADR, K562 / VCR multi-drug resistant cell lines are high expression of P-gp protein as the main drug resistance mechanism, induced by Zhejiang University Cancer Institute, KBV200 multi-drug resistant cell lines are P-gp High protein expression is the main mechanism of drug resistance, purchased from the Institute of Hematology, Chinese Academy of Medical Sciences.

[0057] Reagents: Compound Schizandrin B, dissolved in DMSO, made into 1 mg / ml as mother solution, and then made into working solution with RPMI-1640; all the above reagents were purchased from China National Institute of Pharmaceutical and Biological Products. RPMI-1640 medium and calf serum are products of Gibco in the United States; tetramethylazolium salt (MTT) tetramethylazolium salt (MTT) is a product of Sigma Company, dimethyl sulfoxide (dimethyl sufoxide, DMSO) Imported and packaged f...

Embodiment 3

[0086] Example 3: Toxic effects of Schizandrin B on various cells

[0087] (1) Experimental materials:

[0088] Cell lines and normal cells: K562 and KB cells were provided by Zhejiang University Cancer Institute; K562 / ADR and K562 / VCR multi-drug resistant cell lines have high expression of P-gp protein as the main drug resistance mechanism, provided by Zhejiang University Cancer Institute Induced establishment of lines, multi-drug resistant cell lines with high expression of P-gp protein as the main drug resistance mechanism, etc. have strong drug resistance; KBv200 multi-drug resistant cell lines have high expression of P-gp protein as the main drug resistance The mechanism, cited from the Institute of Materia Medica, Chinese Academy of Medical Sciences, has cross-resistance to multiple drugs.

[0089] Reagent: Compound Schizandrin B, dissolved in DMSO, made into 1 mg / ml as mother solution, and then made into working solution with RPMI-1640; purchased from China Institute f...

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PUM

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Abstract

The invention discloses the application of schisandrin B in the preparation of tumor treating drugs, especially in the preparation of tumor cell multidrug resistance reversal agents and tumor chemotherapy drugs. The compound can also be used in combination with other drugs to further improve the reversal effect. Schizandrin B can effectively reverse the multidrug resistance of tumors. The compound acts on P-glycoprotein and can completely inhibit the function of P-glycoprotein, so it has clinical application significance; in addition, Schizandrin B has low toxicity, but It is toxic to certain tumor cells, so Schizandrin B has good application prospects in clinical tumor chemotherapy, especially as a drug for reversing multidrug resistance of tumor cells.

Description

(1) Technical field [0001] The present invention relates to a new application of schisandrin B, that is, the application of schisandrin B in the preparation of drugs for treating tumors, especially the application of schisandrin B in the preparation of tumor cell multidrug resistance reversal agents and tumor chemotherapy drugs. (2) Background technology [0002] Tumor is one of the main causes of human death, and tumor chemotherapy is a major means of treating tumors. However, cancer chemotherapy will fail because tumor cells acquire drug resistance. [0003] In the "Journal of North China Coal Medical College" in March 2001, Volume 3, No. 2, "The Fluidity Changes of Membrane Lipids and the Research Progress of Tumor Multidrug Resistance Reversal Agents" edited by Zhang Huizhu et al. reported: Tumor cell multidrug resistance ( multidrugresistance, MDR) is one of the main reasons for the failure of chemotherapy. The mechanism of MDR is complex, except that it is mainly rel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/335A61K31/34A61K31/522A61K31/704A61K31/7048A61K31/7072A61K45/06
CPCA61K31/335A61K31/34A61K31/522A61K31/704A61K31/7048A61K31/7072A61K45/06A61P35/00A61K2300/00
Inventor 胡汛王弢
Owner 高陈勇
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