Method for controlling the quality of schisandra raw material fingerprint in the plant medicine for improving hemorheology
A quality control method and fingerprint technology, which are applied in the field of raw material quality control of Schisandra chinensis, can solve the problems of lack of quality control of traditional Chinese medicinal materials and imperfection, and achieve the effects of reliable method, strong specificity and stable medicinal quality.
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[0065] Schisandra Medicinal Material: Northeast China (10 batches)
[0066] equipment name
model
Electronic balance
AL104
METTLER TOLEDO
High performance liquid chromatography
Agilent 1100series
Agilent
Column
Luna C 18 250×4.6cm
temperate box
TC-100
T&D
Circulating water multi-purpose vacuum pump
SHZ-D(3)
Gongyi Yuhua Instrument Co., Ltd.
ultrasonic cleaner
KQ-50E
Kunshan Ultrasonic Instrument Co., Ltd.
[0067] Weigh 0.5g schisandra medicinal material powder, add 20ml 80% methanol, weigh, ultrasonically extract for 50min, make up the lost weight, and filter through 0.45μm microporous membrane to obtain the test solution. Chromatographic conditions: column temperature: 35°C, detection wavelength: 254nm, injection volume: 20μl, chromatographic column: Eclipse (150×4.6mm, 5μm), elution conditions (see Table 1)
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