Method for extracting, separating and preparing lignin monomers from schisandra chinensis

A technology of schisandra and lignans, applied in the field of natural medicinal chemistry, to achieve the effect of technological innovation and improvement, simple extraction steps, and high separation and purification efficiency

Active Publication Date: 2013-12-25
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But so far there is no report on the separation and purification of lignan monomers in Sch

Method used

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  • Method for extracting, separating and preparing lignin monomers from schisandra chinensis
  • Method for extracting, separating and preparing lignin monomers from schisandra chinensis
  • Method for extracting, separating and preparing lignin monomers from schisandra chinensis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1: Supercritical fluid extraction

[0051] Take the fruits of Schisandra chinensis and crush them to obtain schisandra powder with a particle size of 50-65 mesh. Take 2g of schisandra powder and put it in a supercritical fluid extraction tank. The extraction temperature is 40℃, the extraction pressure is 25MPa, and CO 2 The flow rate is 2L / min, the extraction time is 1h, and the crude schisandra extract is obtained. Taking Schisandrin A as a representative, the extraction rate is calculated to be 0.5294%.

Embodiment 2

[0052] Example 2: Supercritical fluid extraction

[0053] Take the fruits of Schisandra chinensis and crush them to obtain Schisandra medicinal powder with a particle size of 10-20 mesh. Take 3g of Schisandra medicinal powder and put it into a supercritical fluid extraction tank. The extraction temperature is 35℃, the extraction pressure is 14MPa, and CO 2 The flow rate is 2L / min, the extraction time is 2h, and the crude schisandra extract is obtained. Taking Schisandrin A as a representative, the extraction rate was calculated to be 0.3926%.

Embodiment 3

[0054] Example 3: Supercritical fluid extraction

[0055] Take the fruits of Schisandra chinensis and crush them to obtain Schisandra medicinal powder with a particle size of 50-65 mesh. Take 3g of Schisandra medicinal powder and put it into a supercritical fluid extraction tank. The extraction temperature is 35℃, the extraction pressure is 30MPa, and CO 2 The flow rate is 2L / min, the extraction time is 2h, and the crude schisandra extract is obtained. Taking Schisandrin A as a representative, the extraction rate is calculated to be 0.6001%.

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Abstract

The invention discloses a method for extracting, separating and preparing lignin monomers from schisandra chinensis. The method comprises the following steps: performing supercritical extraction on schisandra chinensis medicinal powder by using CO2 to obtain a crude extract; performing primary high-speed counter-current chromatographic separation on the crude extract; forming a solvent system A by using hexane, ethyl acetate, methyl alcohol and water in the volume ratio of (2-10):(0-10):(0-10):5, wherein the upper phase A is a stationary phase A and the lower phase A is a mobile phase A; preparing a first schizandrol, a second schizandrol and a third schizandrol; recovering the separated stationary A to serve as a sample to be tested; performing secondary high-speed counter-current chromatographic separation; forming a solvent system B by using hexane, ethyl acetate, methyl alcohol and water in the volume ratio of (1-10):(0-4):(0-9):1, wherein the upper phase B is a stationary phase B, and the lower phase B is a mobile phase B; preparing deoxyschizandrin and schisandrin b. By adopting the method which integrates supercritical extraction and high-speed counter-current chromatographic separation, the deoxyschizandrin and the schisandrin b can be directly used for separating and purifying subsequent monomers without complex sample treatment after the extraction; the steps of extracting and separating are simple and efficient.

Description

Technical field [0001] The invention belongs to the field of natural medicine chemistry, and relates to a fast and efficient combined preparation method for extracting, separating and purifying lignan monomer components from Schisandra. It specifically relates to the use of supercritical extraction combined with high-speed countercurrent chromatography to extract and separate the monomer components of Schisandra. Background technique [0002] Schisandra is the mature fruit of Schisandra chinensis (Turcz.) Baill or Schisandra sphenanthera Rehd.et Wils. The former is called "Northern Schisandra" and the latter is called "Southern Schisandra". It was first published in "Shen Nong's Materia Medica" and was listed as top grade. Schisandra is warm in nature, sweet and sour in taste, and belongs to the lung, heart and kidney meridians. It has the effects of astringent, astringent, nourishing qi, nourishing body fluid, nourishing the kidney and calming the heart. It can be used to tr...

Claims

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Application Information

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IPC IPC(8): C07D317/70C07C43/21C07C43/23C07C41/34C07C41/36C07B63/00
CPCY02P20/54
Inventor 楚楚颜继忠章诗迪童胜强李行诺
Owner ZHEJIANG UNIV OF TECH
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