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Method for extracting deoxyschisandrin and schisandrin B from schisandra

A technology for Schisandrin A and Schisandrin B, which is applied in the field of extracting active ingredients, can solve problems such as inconvenience and low solid yield, and achieve the effect of high extraction rate

Inactive Publication Date: 2011-05-11
GUILIN NATURAL INGREDIENTS CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using this method for extraction and purification, the loss of active components of Schisandra chinensis is small, and the yield of solids is low, but the obtained Schizandrin A and Schisandrin B are mixed in the extract and have not been separated, which requires subsequent production. It is inconvenient to use A or B alone

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] 1) Take Schisandra chinensis and break, add 85% (v / v) aqueous ethanol solution equivalent to 7 times the weight of the raw material, boil and heat-preserve for extraction for 2 hours, filter out the medicinal liquid, and then add 85% (v / v) equivalent to 10 times the weight of the raw material ) ethanol aqueous solution was boiled and kept for extraction for 1 hour, filtered, and the extract was combined, and the extract was concentrated to 2 times the weight of the raw material to obtain a concentrated solution;

[0016] 2) adding absolute ethanol to the concentrated solution so that the concentration of ethanol therein is 60% (by mass), centrifuging, and collecting the supernatant;

[0017] 3) Pass the supernatant through a DM132 type macroporous resin column, wash the column with 1.5 times the resin weight and 55% (v / v) ethanol aqueous solution, collect the eluent A, recycle the ethanol, and dry to obtain the Schisandrin A extract ;

[0018] 4) Elute with 3.5 times t...

Embodiment 2

[0021] 1) Take Schisandra chinensis and crush, add 90% (v / v) aqueous ethanol solution equivalent to 8 times the weight of the raw material, boil for heat preservation and extract for 1 hour, filter out the medicinal liquid, and then add 80% (v / v) equivalent to 8 times the weight of the raw material ) of the ethanol aqueous solution was boiled and kept for extraction for 1 hour, filtered, and the extract was combined, and the extract was concentrated to 3 times the weight of the raw material to obtain a concentrated solution;

[0022] 2) Add 95% (v / v) ethanol to the concentrated solution to make the ethanol concentration 65% (mass), let it cool and then centrifuge to collect the supernatant;

[0023] 3) Pass the supernatant through a D101 type macroporous resin column, wash the column with 2 times the resin weight and 65% (v / v) ethanol aqueous solution, collect the eluate A, recycle the ethanol, and dry to obtain the Schizandrin A extract ;

[0024] 4) Elution with 2.5 times o...

Embodiment 3

[0027] 1) Take Schisandra chinensis and break it, add 95% (v / v) aqueous ethanol solution equivalent to 9 times the weight of the raw material, boil for heat preservation and extract for 2 hours, filter out the medicinal liquid, and then add 85% (v / v) equivalent to 7 times the weight of the raw material ) of the ethanol aqueous solution was boiled and kept for extraction for 1.5 hours, filtered, and the extract was combined, and the extract was concentrated to 2.5 times the weight of the raw material to obtain a concentrated solution;

[0028] 2) Add 90% (v / v) ethanol to the concentrated solution to make the ethanol concentration 65% (mass), let it cool and then centrifuge to collect the supernatant;

[0029] 3) The supernatant is passed through a HP-20 type macroporous resin column, and the column is washed with 2.5 times the weight of the resin and 60% (v / v) ethanol aqueous solution, the eluent A is collected, the ethanol is recovered, and then the equivalent eluent is added ...

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PUM

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Abstract

The invention discloses a method for extracting deoxyschisandrin and schisandrin B from schisandra, comprising the following steps: 1) crushing the schisandra, extracting with 80-95% ethanol by volume concentration, concentrating extracting solution to obtain concentrated solution; 2) adding ethanol in the concentrated solution and ensuring that the concentration of ethanol is 60-65% by weight, centrifuging and collecting supernate; 3) passing the supernate through a macroporous resin column, eluting with 55-65% ethanol by volume concentration, wherein the weight of ethanol is 1.5-2.5 times of that of the resin, collecting eluent A, and drying to obtain deoxyschisandrin extract; and 4) eluting with 85-95 % ethanol by volume concentration, wherein the weight of ethanol is 2.5-3.5 times of that of the resin, collecting eluent B, and drying to obtain schisandrin B extract. By using the method in the invention, the deoxyschisandrin and schisandrin B are eluted in turn, so that the deoxyschisandrin and schisandrin B are separated, wherein the content of the deoxyschisandrin is 0.8-1.2%, and the content of the schisandrin B is 3-4%.

Description

technical field [0001] The invention relates to a method for extracting active components from schisandra, in particular to a method for extracting schisandrin A and schisandrin B from schisandra. Background technique [0002] Schisandra chimesmsis (Schisandra chimesmsis (Turcz.) Baill.) is the dried and mature fruit of Magnoliaceae plant Schisandra chimesmsis (Turcz.) Baill. Schisandra chinensis is an authentic medicinal material that grows in Northeast China and spreads across Xiaoxing'an Mountains, Yichun, Taoshan, Yanbian and other places. Modern research has proved that the bioactive components of Schisandra chinensis are mainly lignans and volatile oils, which have various pharmacological effects on the nervous system and digestive system, among which lignans have various activities such as liver protection, enzyme reduction, and anti-AIDS. The content of total lignans in Schisandra chinensis is 2-8%, and most of its components have the mother nucleus of biphenylcyclo...

Claims

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Application Information

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IPC IPC(8): C07C43/21C07D317/70C07C41/36A61K36/57
Inventor 宋云飞梁远盛赵军孙步祥汤凌志
Owner GUILIN NATURAL INGREDIENTS CORP
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