Detection method of functional effective components of radix seu caulis schisandrae propinquae and application

A technology of active ingredients and detection methods, which is applied in the field of detection of functional active ingredients of Xiaoxueteng, can solve the defects of quality control methods of Chinese patent medicines, does not include Xiaoxueteng quality control methods, and cannot effectively control the quality of Xiaoxueteng medicinal materials. and other problems, to achieve the effect of good linear relationship, good precision and good repeatability

Active Publication Date: 2018-05-01
HUAIHUA UNIV
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

However, the Chinese Pharmacopoeia does not include the quality control methods and functional active ingredient detection indicators of S. sagelifolia, which c

Method used

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  • Detection method of functional effective components of radix seu caulis schisandrae propinquae and application
  • Detection method of functional effective components of radix seu caulis schisandrae propinquae and application
  • Detection method of functional effective components of radix seu caulis schisandrae propinquae and application

Examples

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Embodiment 1

[0037] A method for detecting the functional active ingredients of Spatholobus, comprising the following steps:

[0038] S1, prepare standard solutions of rutin, schisandrin A and schisandrin B respectively:

[0039] (1) Preparation of rutin standard solution: Accurately weigh 0.0091 g of rutin standard into a 10 mL volumetric flask, add methanol to make up to 10 mL, and obtain 0.91 mg / mL rutin standard solution.

[0040](2) Preparation of schisandrin standard substance solution: Accurately weigh 0.0043 g of schisandrin standard substance into a 10 mL volumetric flask, add methanol to constant volume to 10 mL, and obtain 0.43 mg / mL schisandrin standard substance solution.

[0041] (3) Preparation of Schizandrin standard substance solution: Accurately weigh 0.0010 g of Schisandrin standard substance into a 10 mL volumetric flask, add methanol to dilute to 10 mL, and obtain 0.10 mg / mL Schisandrin standard substance solution.

[0042] S2, preparation of the test solution

[004...

Embodiment 2

[0048] A method for detecting the functional active ingredients of Spatholobus, the steps are basically the same as in Example 1, the difference is that in S2, the mass of Spatholobus is 10.1235g, the amount of methanol added is 45ml, and the ultrasonic time is 40min.

Embodiment 3

[0050] A method for detecting the functional active ingredients of Spatholobus, the steps are basically the same as in Example 1, the difference is that in S2, the mass of Spatholobus is 10.4751g, the amount of methanol added is 55ml, and the ultrasonic time is 35min.

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Abstract

The invention belongs to the technical field of the detection of components of traditional Chinese medicines, and discloses a detection method of effective components of radix seu caulis schisandrae propinquae and application. The detection method comprises steps of preparation of standard substance solutions of rutin, schizandrin A and schisandrin B respectively, preparation of a test sample solution, HPLC (High Performance Liquid Chromatography) detection and the like, wherein chromatographic conditions of the HPLC detection are as follows: a methanol-1 percent glacial acetic acid solution is used as mobile phase eluent; the gradient elution is carried out for 90min; volume fractions of methanol in the eluent corresponding to each time interval of the elution are respectively: 20 percentduring 0min to 20min, 40 percent during 20min to 40min, 60 percent during 40min to 60min, 80 percent during 60min to 80min and 100 percent during 80min to 90min; a flow speed is 1mL/min; a detectionwavelength is 257nm. The detection method uses the HPLC detection with the gradient elution, can be used for measuring three functional effective components in the radix seu caulis schisandrae propinquae at the same time, and can be used for more completely reflecting a pharmacodynamic effect of the radix seu caulis schisandrae propinquae.

Description

technical field [0001] The invention belongs to the technical field of detection of components of traditional Chinese medicines, and in particular relates to a detection method and application of functional effective components of Caulis Spatholobus. Background technique [0002] Schisandra Propinqua (Wall.) Baill.Var.Sinensis Oliv., Dchisandrapropingua var.sinensis, also known as Tie hoopsan, is a deciduous woody vine of the Magnoliaceae Schisandra genus. It is used as medicine with roots, canes and leaves. It has been reported in the literature that Spatholobus mainly contains enshicine, isosschizandrolic acid, deoxyschizandrin, β-sitosterol, and stearic acid. and other chemical components. We found that it contains more rutin, schisandrin A and schisandrin B by thin layer chromatography. Rutin, schisandrin and schisandrin B are medicinal ingredients with definite efficacy. They are the quality control indicators of many traditional Chinese medicines in the Chinese Phar...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 赵子剑赵畅梁玉兰诸葛树彬刘小波严丽莹王斌
Owner HUAIHUA UNIV
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