Method for purifying schizandrin and schisandrin b from schisandra extract

A technology of schisandra extract and schisandra alcohol A, which is applied in ether separation/purification, organic chemistry, ether preparation, etc., can solve the problems of high cost, complicated operation of the purification method, easy residual toxic and harmful substances, etc., and achieve low cost and good quality Prospect of medicine and health food, effect of reducing dosage

Active Publication Date: 2015-09-23
劲牌持正堂药业有限公司
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Problems solved by technology

[0005] The purpose of the present invention is to solve the current purification methods of schisandrin A and schisandrin B with complex operation, high cost, and easy residual toxic and harmful substances, and provide a simple operation, safe and non-toxic, low cost, high recovery rate, And the method that can simultaneously purify schisandrin A and schisandrin B has good prospects for the development of medicine and health food

Method used

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  • Method for purifying schizandrin and schisandrin b from schisandra extract
  • Method for purifying schizandrin and schisandrin b from schisandra extract
  • Method for purifying schizandrin and schisandrin b from schisandra extract

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Embodiment 1

[0026] A method for purifying schisandrin A and schisandrin B from Schisandra chinensis extract of the present embodiment comprises the following steps:

[0027] (1) Extraction: add 800L of pure water to 100Kg of schisandra crude drug, reflux and extract at 70°C for 2 hours, collect the medicinal residue by filtration, and discard the filtrate; add 800L of 90% (v / v) ethanol to the medicinal residue each time Aqueous solution, reflux extraction at 80°C for 2 times, 2 hours each time, collect each extract and detect the content of schisandrin A and schisandrin B, the detection spectrum is shown in image 3 , figure 2 It is the spectrogram of the standard substance of schisandrin A and schisandrin B;

[0028] (2) Concentration: Concentrate the extract to 50L, then add 300L of pure water to the concentrated solution, cool it at 0-4°C and let it stand for 5 hours for water sinking, and perform the first centrifugation of the water sinking treatment liquid, The centrifugal speed ...

Embodiment 2

[0034] see figure 1 , the present invention comprises the following steps:

[0035](1) Extraction: Add 500L of 95% (v / v) ethanol aqueous solution to 100Kg Schisandra crude drug each time, reflux extraction at 90°C for 4 times, each time for 2 hours, and collect the extracts each time;

[0036] (2) Concentration: Concentrate the extract to 80L, then add 400L of pure water to the concentrated solution, cool it at 0-4°C and let it stand for 4 hours for water sinking, and perform the first centrifugation of the water sinking treatment liquid, The centrifugal speed is 3000r / m, and the precipitate and supernatant are obtained after centrifugation. 90-95% of schisandrin and 50%-55% of schisandrin enter into the precipitate, 5-10% of schisandrin and 45-50% The schisandra alcohol A stays in the supernatant, and the precipitation is to be processed;

[0037] (3) Adsorption: Add 10Kg of activated carbon to the supernatant for adsorption, stir and absorb for 2 hours, then perform the se...

Embodiment 3

[0041] see figure 1 , the present invention comprises the following steps:

[0042] (1) Extraction: add 1000L of 70% (v / v) ethanol aqueous solution to 100Kg Schisandra crude drug each time, reflux extraction at 50°C for 2 times, each time for 3 hours, and collect the extraction solution each time;

[0043] (2) Concentration: Concentrate the extract to 30L, then add 200L of pure water to the concentrated solution, cool it at 0-4°C and let it stand for 6 hours for water sinking, and perform the first centrifugation of the water sinking treatment liquid, The centrifugal speed is 10000r / m. After centrifugation, the precipitate and supernatant are obtained. 90-95% of Schizandrin B and 50%-55% of Schizandrin A enter the precipitate, 5-10% of Schisandrin B and 45-50% The schisandra alcohol A stays in the supernatant, and the precipitation is to be processed;

[0044] (3) Adsorption: Add 20Kg of activated carbon to the supernatant for adsorption, stir and absorb for 3 hours, then pe...

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Abstract

The invention relates to a method for purifying schizandrin and schisandrin b from a schisandra extract. The method comprises the following steps: extracting a schisandra crude drug, collecting an extracting solution, concentrating the extracting solution to 0.3-0.8 time of the weight of the crude drug, adding purified water with the weight being 2-4 times of that of the crude drug in a concentrated solution, cooling under 0-4 DEG C and standing for 4-6 h for water precipitation, performing first high-speed centrifugation separation, and precipitating to wait treatment; adding 10% to 20% active carbon with the weight of the crude drug to a supernate, stirring for adsorption for 0.5-3 h, performing second high-speed centrifugation separation, and centrifuging the supernate for rejection; adding 60% to 95% (v / v) ethanol solution with the weight being 4-20 times of that of the crude drug to active carbon, heating to 50-80 DEG C for elution for 1-3 h, performing third high-speed centrifugation separation, and collecting the supernate; and adding the supernate for sedimentation after first centrifugation, stirring, homogenizing, adding auxiliary materials with the weight being 3% to 8% of the weight of the crude drug, and drying to obtain a dry extract containing the schizandrin and the schisandrin b. The method is simple in operation, safe, non-toxic, low in cost and high in recovery rate, can recycle the schizandrin and the schisandrin b simultaneously, and has good development prospects of medicine and health food.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine preparation, in particular to a method for purifying schisandrin A and schisandrin B from schisandra extract. Background technique [0002] Schisandra chinensis (Turcz.) Baill., a traditional Chinese medicine, is the dry and mature fruit of Schisandra chinensis (Turcz.) Baill. It has the effects of astringent and astringent, nourishing qi and promoting body fluid, invigorating the kidney and calming the heart. It is often used for chronic cough and shortness of breath, nocturnal emission, enuresis, frequent urination, chronic diarrhea, spontaneous sweating, thirsty wounds, internal heat and quenching thirst, palpitations and insomnia. [0003] Schisandra contains schisandrin A, schisandrin A, schisandrin B, schisandra C and other lignans, which have good liver protection and enzyme-lowering effects, and are often used in traditional Chinese medicine and health food, such as S...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C43/23C07C41/34C07D317/70
CPCC07C41/34C07C2603/36C07D317/70C07C43/23
Inventor 张雪元周彬杨跃军汪陈平吴建辉杨波
Owner 劲牌持正堂药业有限公司
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