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Application of schisandrin B in preparation of diluted liquid for preserving boar semen

A technique for schisandra B and swine semen is applied in the field of dilution of swine semen stored at room temperature and its preparation field, which can solve the problems of high cost and different application effects, and achieve the effects of reliable preservation effect and good preservation effect.

Active Publication Date: 2015-07-15
NORTHWEST A & F UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In addition, there are many kinds of dilution and storage formulas for pig semen at home and abroad, most of which have the disadvantages of inconsistent application effects and high costs, which bring a lot of inconvenience to production plants, grass-roots breed improvement stations and professional households

Method used

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  • Application of schisandrin B in preparation of diluted liquid for preserving boar semen
  • Application of schisandrin B in preparation of diluted liquid for preserving boar semen
  • Application of schisandrin B in preparation of diluted liquid for preserving boar semen

Examples

Experimental program
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Effect test

Embodiment

[0020] (1) Diluent preparation for porcine semen stored at room temperature

[0021] The diluent used for the storage of pig semen at room temperature given in this example is an aqueous solution, and every 1L of the aqueous solution contains the following substances:

[0022] Glucose: 35.0~40.0g, Sodium bicarbonate: 0.5~1.0g, Sodium citrate: 6.0~7.0g, EDTA: 1.0~1.5g, Citric acid: 0.1~0.5g, Potassium chloride: 0.5~1.0g, Qing Damycin: 4×10 6 IU, SchB: 10 μmol. The preparation method comprises the following steps:

[0023] (1) Take glucose, sodium bicarbonate, sodium citrate, EDTA, citric acid, potassium chloride, gentamicin, Sch B by formula for subsequent use;

[0024] (2) Glucose, sodium bicarbonate, sodium citrate, EDTA, citric acid, and potassium chloride were prepared into an aqueous solution with 1L double-distilled water;

[0025] (3) After the components in the aqueous solution are fully dissolved, add the formulated amount of gentamycin and Sch B to obtain a diluti...

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Abstract

The invention relates to an application of schisandrin B in the preparation of a diluted liquid for preserving boar semen. The invention also relates to a diluted liquid for preserving boar semen. The diluted liquid comprises glucose, sodium bicarbonate, sodium citrate, EDTA, critic acid, potassium chloride, gentamycin, and schisandrin B. The effect of the provided diluted liquid for preserving boar semen is good and reliable. The high quality semen stored at a room temperature can be provided for pig artificial insemination. After the boar semen is preserved in the diluted liquid at a temperature lower than 17 DEG C for five days, the semen activity is still above 0.6.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a diluent for storing animal semen at room temperature, in particular to a diluent for storing porcine semen at room temperature and a preparation method thereof. Background technique [0002] With the promotion and application of pig artificial insemination technology in intensive pig farms, more and more attention has been paid to the preservation technology of semen, and it has increasingly shown that it can reduce the amount of boar breeding and feeding costs, accelerate the improvement of pig breeds, There are many advantages in reducing the spread of disease. Compared with frozen semen, semen preserved in liquid state at room temperature has the advantages of good reproductive performance of sows (high conception rate, farrowing rate and litter size), low cost and simple operation procedures after artificial insemination; from the perspective of semen preservation effect , The s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
Inventor 胡建宏刘琦苏泽智何锦朱林炜李亚新王立强杨公社梁国栋贾永宏
Owner NORTHWEST A & F UNIV
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