Method for extracting Schisandrin B from Schisandra chinensis

A technology of schisandra B and schisandra, applied in the direction of organic chemistry, etc., can solve the problems of limited production capacity and difficult industrial production technology.

Active Publication Date: 2013-10-16
梦阳药业上海有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The implementation of this method requires special equipment such as high-speed countercurrent chromatography, and the industrial production technology is difficult and the production capacity is limited.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1: precipitating agent preferred test

[0018] The composition of the ethanol extract of Schisandra chinensis is relatively complex, containing a lot of water-soluble components such as tannins and organic acids. Such impurities pollute the resin column and affect the service life of the resin. Therefore, it is considered to add precipitating agents during the extraction of medicinal materials to reduce their content in the extraction solution. In this experiment, the amount of schisandrin in the medicinal material extract prepared by adding different precipitating agents and the weight of the dry paste were used as the investigation indicators to analyze whether the addition of precipitating agents was reasonable and appropriate.

[0019] Extraction method: Take 5g of schisandra powder (batch number: SC03) (or the mixture of schisandra powder and precipitant) in a round bottom flask, add 80% EtOH 40ml (8 times the amount), soak for 1hr, stir in 80°C water ba...

Embodiment 2

[0028] 1. Medicinal material extraction: Take 500g of Schisandra chinensis medicinal material, crush it into coarse grains, add 50g of calcium oxide, add 80% ethanol and soak at 80°C, stir and extract twice, each time for 3hr, the first time is 8 times the amount (4L), the second time 6 times the amount (3L), combined the two alcoholic extracts, recovered ethanol under reduced pressure, and obtained the concentrated alcoholic extract of Schisandra chinensis (1g crude drug / ml).

[0029] 2. Macroporous adsorption resin column chromatography: take the concentrated solution of Schisandra chinensis alcohol extraction, and load the sample on the AB-8 type that has been processed (macroporous adsorption resin 350g, 95% ethanol wet filling, 60% ethanol pre-balanced 1500ml) (Tianjin Nankai Hecheng Technology Co., Ltd.) on a macroporous adsorption resin column (1BV=500mL), let it stand for 1hr, add 60% ethanol to elute 4BV, add 80% ethanol to elute 8BV, collect the fractions, and combine...

Embodiment 3

[0033] 1. Medicinal material extraction: Take 500g of Schisandra chinensis, crush it into coarse grains, add 50g of calcium hydroxide, add 85% ethanol to reflux at 90°C for 2 times, each time for 3 hours, the first time 6 times (3L), the second time 4 times Double the amount (2L), combine the two alcohol extracts, and recover under reduced pressure to obtain the concentrated alcohol extract of Schisandra chinensis (1g crude drug / ml).

[0034]2. Macroporous adsorption resin column chromatography: take the concentrated solution of Schisandra chinensis alcohol extraction, load the sample on the treated NKA macroporous adsorption resin column (1BV=500mL), let it stand for 2hr, add 60% ethanol to elute 4BV, Add 85% ethanol to elute 8BV, collect the fractions, combine the fractions containing Schizandrin B to obtain the front and pure fractions of Schisandrin B, combine the pure fractions of Schizandrin B to recover the solvent under reduced pressure, and add an appropriate amount of...

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PUM

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Abstract

The invention provides a method for extracting Schisandrin B from Schisandra chinensis. The method adopts macroporous adsorption resin column chromatography, silica gel column chromatography and recrystallization to extract and separate Schisandrin B from the Schisandra chinensis medicine in order to obtain high-purity Schisandrin B, and accords with the technological characteristics of industrial production. The method has the following advantages: the purity of the prepared Schisandrin B can reach 90-100%, and the method can be applied to the industrial large-scale preparation of the Schisandrin B.

Description

(1) Technical field [0001] The invention relates to a method for extracting and separating schisandrin B from schisandra chinensis. (2) Background technology [0002] Schisandra chinensis is the dry and mature fruit of Schisandra chinensis, a plant of the Magnoliaceae family. The lignans with biphenylcyclooctadiene structure in Schisandra chinensis are the main bioactive components, mainly including Schizandrin B, Schisandrin A, Schisandrin A, etc., and the content of Schisandrin B is generally 0.1-0.4 %between. In recent years, studies have found that Schizandrin B has various biological activities such as anti-tumor, liver protection, anti-ulcer, anti-virus, and has a good effect on scavenging free radicals. Therefore, the development and application of Schizandrin B as a medicinal raw material has important research value. However, there are few sources of schisandrin, and it is mainly obtained by extracting and purifying Schisandra chinensis, and the content of schisa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D317/70
Inventor 汪豪张敏曹涛田文桥
Owner 梦阳药业上海有限公司
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