Method for extracting and purifying anthocyanins from blueberry red leaves
An anthocyanin and red leaf technology, applied in chemical instruments and methods, organic chemistry, azo dyes, etc., can solve problems such as extraction and purification of anthocyanins that have not been independently studied, and achieve easy industrial production, low production cost, and simple methods. Effect
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Embodiment 1
[0021] Embodiment 1 of the present invention: a method for extracting and purifying anthocyanins from the red leaves of Jatu blueberry, (1) select fresh red leaves of Jatu blueberry, remove diseased insects and rotten leaves, wash them with clean water, and put them under 0.1 atmospheric pressure for 60 Dry at ℃ for 16 hours, crush the dried blueberry red leaves to pass through a 60-mesh sieve to obtain blueberry red leaf coarse powder, and store the obtained blueberry red leaf coarse powder in a sealed dry store for subsequent use; add mass percentage concentration of 80% ethanol water solution, the liquid-to-solid ratio of ethanol water solution and blueberry red leaf coarse powder is 1:20, and 2 auxiliary extractions are carried out at room temperature with 300W ultrasonic power, each extraction time is 40min, and the obtained extraction solution is filtered to remove slag Finally, under the condition of 0.1 atmospheric pressure and 35°C, it was concentrated under reduced pr...
Embodiment 2
[0022] Embodiment 2 of the present invention: a method for extracting and purifying anthocyanins from the red leaves of blueberry and blueberry, selecting fresh red and blueberry leaves of garden blueberry, and removing the rotten leaves from diseased insects, washing them with clear water, and drying them at 60°C for 24 hours under 0.1 atmospheric pressure. Hour, the blueberry red leaf after drying is pulverized to cross 60 mesh sieves, obtains blueberry red leaf coarse powder; Add mass percent concentration in the blueberry red leaf coarse powder and be the ethanol aqueous solution of 75%, the liquid-to-material ratio of ethanol aqueous solution and blueberry red leaf coarse powder 1 :20, and use 250W ultrasonic power at room temperature to carry out 3 auxiliary extractions, each extraction time is 70min, after the obtained extraction solution is filtered to remove slag, it is concentrated under reduced pressure at 38°C under 0.1 atmospheric pressure to no ethanol , and then ...
Embodiment 3
[0023] Embodiment 3 of the present invention: the method for extracting and purifying anthocyanins from the red leaves of Baldwin blueberry, select fresh red leaves of Baldwin blueberry, and remove the rotten leaves of diseased insects, wash them with clean water, and heat them at 60°C under 0.08 atmospheric pressure Dry for 20 hours, crush the dried blueberry red leaves to pass through a 60-mesh sieve to obtain blueberry red leaf coarse powder; add ethanol aqueous solution with a mass percentage concentration of 80% to the blueberry red leaf coarse powder, and the liquid material of ethanol aqueous solution and blueberry red leaf coarse powder Ratio 1:30, and 250W ultrasonic power was used at room temperature for 3 auxiliary extractions, each extraction time was 40min, the obtained extraction solution was filtered and slag removed, and concentrated under reduced pressure at 40°C under 0.08 atmospheres to no Contain ethanol, then dissolve in 80 times pure water to obtain bluebe...
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