Method for separating and purifying placental blood by using protective fluid and placenta squeezer and method for resuscitating placental blood

A technology of extruder and protective liquid, which is applied in sterilization method, cell dissociation method, biological material sampling method, etc., can solve the problems of long preparation time, low phenotypic detection value, difficulty in separation and extraction of placental blood, etc. , to reduce the preparation time and reduce the risk of contamination

Active Publication Date: 2020-03-24
天晴干细胞股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to solve the problems of difficult placental blood separation and extraction, low nucleated cell viability, small number of cells, long preparation time, low phenotypic detection

Method used

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  • Method for separating and purifying placental blood by using protective fluid and placenta squeezer and method for resuscitating placental blood
  • Method for separating and purifying placental blood by using protective fluid and placenta squeezer and method for resuscitating placental blood
  • Method for separating and purifying placental blood by using protective fluid and placenta squeezer and method for resuscitating placental blood

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Experimental program
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Effect test

specific Embodiment approach 1

[0023] Specific Embodiment 1: The placenta squeezer in this embodiment includes an extrusion rod 1, a fixed rod 2, an extrusion cylinder 3, an extrusion disc 4, a pressure-resistant net 5, a filter screen 6 and a fixing seat 8; wherein the extrusion rod 1 It is threadedly connected with the fixed rod 2, and the lower end of the extrusion rod 1 is connected with the extrusion disc 4; during the extrusion process, the extrusion disc 4 extends into the interior of the extrusion cylinder 3; the compression-resistant net 5 and filter The net 6 and the anti-pressure net 5 are above the filter screen 6; the bottom of the extruding cylinder 3 is conical, and the bottom end is provided with a pipe connection port 7; Connected to the fixed seat 8, fixed by the set bolt.

[0024] In this embodiment, the extrusion disk 4, the pressure-resistant net 5 and the filter net 6 have the same diameter.

[0025] The fixing seat 8 of the placenta extruder in this embodiment is provided with a tigh...

specific Embodiment approach 2

[0027] Embodiment 2: This embodiment differs from Embodiment 1 in that: the extrusion barrel 3, the extrusion disc 4 and the pressure-resistant mesh 5 are all made of stainless steel. Others are the same as the first embodiment.

specific Embodiment approach 3

[0028] Embodiment 3: This embodiment is different from Embodiment 1 or Embodiment 2 in that: the filter screen 6 is a 100-mesh filter screen. Others are the same as those in Embodiment 1 or 2.

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Abstract

The invention relates to a method for separating and purifying placental blood by using a protective fluid and a placenta squeezer and a method for resuscitating the placental blood, and relates to the method for separating and purifying the placental blood by using the protective fluid and the placenta squeezer and the method for resuscitating the placental blood. The purpose of the invention isto solve the problems of difficult separation and extraction of placental blood, low viability of nucleated cells, low number of cells, easy contamination during preparation, and reduced cell qualityduring resuscitation in a conventional method. The placenta squeezer provided by the invention is used, and the protective fluid is added, so that the blood in a placenta is fully squeezed out, and through the efficient separation and purification method, the purposes that the total number of the nucleated cells can reach 15x10<8> or more, the viability is 95% or more, the colony formation is obvious, the phenotype flow detection CD34 reaches 0.3% or more, the pollution rate is controlled within 1%, the viability after resuscitation is 90% or more, the cloning ability is realized, the phenotype flow detection CD34 can reach 0.3% or more, and the sterility test is negative can be ensured. The methods are applied to the field of placental hematopoietic stem cell preparation.

Description

technical field [0001] The invention relates to a method for separating, purifying and resuscitating placental blood by using a protective solution and a placenta squeezer. Background technique [0002] Placenta-derived hematopoietic stem cells are a group of primitive hematopoietic cells present in the placenta tissue. Hematopoietic stem cells are highly undifferentiated cells. It can also be said that they are the original cells of all blood cells (most of which are immune cells). [0003] Transplantation can treat malignant blood diseases, some malignant tumors, some genetic diseases and other 75 fatal diseases Acute leukemia, chronic leukemia, myelodysplastic syndrome, hematopoietic stem cell disease, myeloproliferative disease, lymphoproliferative disease, macrophage Diseases, hereditary metabolic diseases, histiocytic diseases, hereditary red blood cell diseases, hereditary immune system diseases, hereditary platelet diseases, plasma cell diseases, thalassemia, non-hem...

Claims

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Application Information

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IPC IPC(8): C12M1/12C12M1/00C12N5/078A01N1/02
CPCC12M33/14C12N5/0634A01N1/0231A01N1/0221A01N1/0284C12N2509/10Y02W30/62
Inventor 王博昊张怡王灵娟刘艳青
Owner 天晴干细胞股份有限公司
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