The invention discloses a preparation method of fructus gleditsiae total saponins. The preparation method is characterized by comprising the following steps of: (1) taking a dried fructus gleditsiae medical material, and
grinding the medical material into coarse
powder; (2) taking the fructus gleditsiae coarse
powder, adding deionized water, decocting and filtering out, extracting for three times, combining filtrate, and carrying vacuum concentration to obtain liquid
medicine; (3) taking the liquid
medicine, extracting with a water saturated n-butyl
alcohol solution for 3 times, combining n-butyl
alcohol extracting solutions, reducing pressure and recovering to obtain an n-butyl
alcohol extract; and (4) taking the n-butyl alcohol extract, adding water for
dissolution, filtering out, collecting filtrate, adding into a D101
macroporous resin column, firstly eluting with
distilled water till the filtrate is near colorless, then eluting with 10%
ethanol till the filtrate is near colorless, finally eluting with 70%
ethanol, collecting 70%
ethanol eluant, and
drying under reduced pressure to obtain the fructus gleditsiae total saponins. The fructus gleditsiae total saponins obtained by the method is light brown orange
powder, acrid and salty in taste and has pungent smell of
saponin; the content of the total saponins is above 80%; and the fructus gleditsiae total saponins can be used for treating acute and
chronic leukemia.