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Preparation method of fructus gleditsiae total saponins

A technology for porcine tooth soap and total saponins, which is applied in the field of preparation of porcine tooth soap total saponins, can solve the problems of low content of total saponins, unsatisfactory efficacy and the like

Inactive Publication Date: 2013-05-08
SHANDONG ACAD OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A Chinese invention patent application with the publication number CN101537036A discloses a saponin extract and its preparation method and application. It has the following defects: the total saponin content in the saponin extract obtained by extraction is not high, and the drug effect is not ideal. room for improvement

Method used

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  • Preparation method of fructus gleditsiae total saponins
  • Preparation method of fructus gleditsiae total saponins
  • Preparation method of fructus gleditsiae total saponins

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The optimization of the preparation method of total saponins of pig tooth soap of embodiment one

[0025] 1. Research data of water decoction extraction

[0026] (1) Determination of decoction conditions: In the optimization of the decoction process of pig tooth soap, the total saponin yield was taken as an index, and the three factors and three levels of orthogonality were carried out on the amount of water added, the number of extractions, and the extraction time that affect the extraction rate. Test, preferably determined suitable water decoction extraction condition, the concrete test method of its orthogonal optimal test is: accurately take pig tooth soap coarse powder 100g, weigh 9 parts altogether, carry out 9 parts respectively by the water decoction condition of test arrangement in table 1 In the second water decoction test, each test solution is concentrated and settled to volume in a 100ml measuring bottle, and the content of total saponins in each extracted ...

Embodiment 2

[0052] The study of the inhibitory effect of total saponins of pig tooth saponins on K562 and NB4 cells of embodiment two

[0053] 1 Experimental materials and instruments

[0054] 1.1 Preparation of pig tooth soap test sample solution:

[0055] The total saponin 1 of pig tooth soap is the 70% ethanol eluate after being applied to the macroporous resin column (the process sample of the present invention), the sample batch number: 20111013. Provided by the research group. Methods as below:

[0056] (1) Pretreatment of pig tooth soap extraction: take the dried pig tooth soap medicinal material, grind it into coarse powder, and set aside;

[0057] (2) Decoction extraction process of the total saponins of pig tooth soap: take the above coarse powder of pig tooth soap, add 10 times the amount (weight multiple) of deionized water, decoct for 2 hours, filter; add 8 times the amount (weight multiple ) water, decoct for 2 hours, and filter; add 8 times the amount (weight multiple)...

Embodiment 3

[0095] Example 3 The research on the inhibitory effect of total saponins of pig tooth saponins on HL-60 cells

[0096] 1 Experimental materials and instruments

[0097] 1.1 Preparation of pig tooth soap test sample solution: Accurately weigh an appropriate amount of total saponins of pig tooth soap (same as in Example 2), and prepare a liquid medicine containing 50 mg per milliliter with water. Sterilize by filtration with a 0.22 μm syringe filter, and it is the test sample of anti-leukemia cells in vitro.

[0098] 1.2 Cisplatin for injection (CDDP): It is a positive control drug for the test, produced by Shandong Qilu Pharmaceutical Factory, 20ml / bottle, batch number 200212024, diluted with normal saline to the required concentration during the test.

[0099] MTT: purchased from Sino-American Biotechnology Company, batch number 20100712.

[0100] DG3022A ELISA: produced by Huadong Electronic Tube Factory.

[0101] Passage cells in vitro: HL-60: human promyelocytic leukemia...

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Abstract

The invention discloses a preparation method of fructus gleditsiae total saponins. The preparation method is characterized by comprising the following steps of: (1) taking a dried fructus gleditsiae medical material, and grinding the medical material into coarse powder; (2) taking the fructus gleditsiae coarse powder, adding deionized water, decocting and filtering out, extracting for three times, combining filtrate, and carrying vacuum concentration to obtain liquid medicine; (3) taking the liquid medicine, extracting with a water saturated n-butyl alcohol solution for 3 times, combining n-butyl alcohol extracting solutions, reducing pressure and recovering to obtain an n-butyl alcohol extract; and (4) taking the n-butyl alcohol extract, adding water for dissolution, filtering out, collecting filtrate, adding into a D101 macroporous resin column, firstly eluting with distilled water till the filtrate is near colorless, then eluting with 10% ethanol till the filtrate is near colorless, finally eluting with 70% ethanol, collecting 70% ethanol eluant, and drying under reduced pressure to obtain the fructus gleditsiae total saponins. The fructus gleditsiae total saponins obtained by the method is light brown orange powder, acrid and salty in taste and has pungent smell of saponin; the content of the total saponins is above 80%; and the fructus gleditsiae total saponins can be used for treating acute and chronic leukemia.

Description

technical field [0001] The invention relates to a preparation method of total saponins of pig tooth saponins. Background technique [0002] Cancer and leukemia (also known as blood cancer) are malignant diseases that seriously threaten human life. They account for the first and second deaths. However, there are still no ideal therapeutic drugs and methods at present. At present, patients with early, middle and advanced cancers often use surgery, surgery plus radiotherapy, chemotherapy, surgery plus Chinese medicine and radiotherapy, chemotherapy plus Chinese medicine and other different therapeutic methods. These methods Although it has a certain therapeutic effect, can partially relieve symptoms and prolong the life of patients, radiotherapy and chemotherapy can easily cause serious trauma to the human body. Among the existing therapeutic drugs, some drugs have relatively large toxic and side effects, some are expensive, and some are expensive. The curative effect is poor,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/483A61P35/02
Inventor 赵渤年张新军贾元印李贵海
Owner SHANDONG ACAD OF CHINESE MEDICINE
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