64 results about "Chondroblast" patented technology

Methods and compositions for directing adipose-derived stromal cells cultivated in vitro to differentiate into cells of the chondrocyte lineage are disclosed. The invention further provides a variety of chondroinductive agents which can be used singly or in combination with other nutrient components to induce chondrogenesis in adipose-derived stromal cells either in cultivating monolayers or in a biocompatible lattice or matrix in a three-dimensional configuration. Use of the differentiated chondrocytes for the therapeutic treatment of a number of human conditions and diseases including repair of cartilage in vivo is disclosed.

This application describes apparatuses and methods for musculoskeletal tissue engineering. Specifically, graft collar and scaffold apparatuses are provided for promoting fixation of musculoskeletal soft tissue to bone.This application provides for graft collars comprising biopolymer mesh and / or polymer-fiber mesh for fixing tendon to bone. In one aspect, the graft collar comprises more than one region, wherein the regions can comprise different materials configured to promote integration of and the regeneration of the interfacial region between tendon and bone.This application also provides for scaffold apparatuses and methods for fixing musculoskeletal soft tissue to bone. The scaffold apparatus is multiphasic, preferably triphasic, and each phase is configured promote growth and proliferation of a different cell and its associated tissue. In one aspect, the scaffold apparatus is triphasic, with phases comprising materials to promote growth and proliferation of fibroblasts, chondroblasts, and osteoblasts. In addition, an apparatus comprising two portions, each of said portion being the scaffold apparatus described above is provided, wherein each of said portion encases one end of a soft tissue graft. Further, a triphasic interference screw is provided.This application further provides apparatuses and methods for inducing formation of fibrocartilage comprising wrapping a graft collar with polymer-fiber mesh configured to apply compression to the graft collar. In another aspect, the polymer-fiber is applied directly to the graft to apply compression to the graft.

The present invention relates to a complex support body for regenerating bone-cartilage, a method for manufacturing thereof, and a composition for treating bone and cartilage related diseases comprising the same as an active ingredient, and more particularly, to a complex support body for regenerating bone-cartilage, which comprises a bone regeneration layer consisting of a biodegradable polymer and a biocompatible ceramic, and a cartilage regeneration layer, which is coupled to the bone regeneration layer and in which cells that can be differentiated into cartilages cells are fixed; a method for manufacturing thereof; and a composition for treating bone and cartilage related diseases comprising the same as an active ingredient. The complex support body of the present invention for regenerating bone-cartilage is manufactured as a three-dimensional support body, which is similar to a living tissue, according to a bioplotting method, and exerts the effect of regeneration into a bone tissue and a cartilage tissue, respectively, depending on materials encountered in the environment where the complex support body for regenerating bone-cartilage is used.

The invention discloses preparation, induced differentiation and application of a self-assembled mesenchymal stem cell aggregate. The mesenchymal stem cell aggregate is prepared through the following steps of (1), coating a porous culture plate by using aquogel under aseptic conditions; (2), carrying out amplification cultivation on mesenchymal stem cells to obtain the third generation; and (3), preparing cell suspension from the third generation of mesenchymal stem cells by using aggregate forming induced liquid, adding the cell suspension into each pore of the coated porous culture plate so that the number of cells in each pore is 1*10<5> to 1*10<6>, changing the aggregate every day to form induced liquid for one time, and putting the porous culture plate in a cell culture box to culture so as to form the mesenchymal stem cell aggregate with the diameter of 500-600mu m. According to the invention, high-density three-dimensional culture of mesenchymal stem cells can be realized without a bracket material; the uniformity of chondroblast induced mesenchymal stem cells is increased; and the mesenchymal stem cell aggregate can be directly used for repairing articular cartilage defects.

The invention provides a biobrick, comprising MSCs, a core layer wrapping the MSCs and a shell layer encapsulating the core layer, wherein the core layer and the shell layer are independently prepared from a biodegradable material and the core layer contains an inducing factor capable of inducing differentiation of MSCs into osteoblasts or bone. The invention also provides another biobrick, comprising MSCs, a core layer wrapping the MSCs and a shell layer encapsulating the core layer, wherein the core layer and the shell layer are independently prepared from a biodegradable material and the core layer contains an inducing factor capable of inducing differentiation of MSCs into chondroblasts or cartilage. The invention further provides a method for preparing a composite structure containing bone and cartilage. The composite structure comprises the above-mentioned two biobricks. The invention also provides the composite structure containing bone and cartilage prepared by using the method.

The invention relates to a method for inducing fibroblasts into chondrocyte like cells, and belongs to the field of cell biology and regenerative medicine. The method aims at the general problems that carrier mediation and transgenic operation are needed, requirements on experimental facilities and technical level are relatively high, the security of produced chondrocytes is relatively poor and the like in methods for transdifferentiation of the fibroblasts into the chondrocytes in the prior art, and uses fish oocyte extract for inducing reprogramming of the fibroblasts into the chondrocyte like cells. The chondrocyte like cells are obtained only by use of the fish oocyte extract for inducing and use of a chondroblast induction medium for directional inducing culture without introduction of exogenous genes, so that experimental operation is relatively simple, and the obtained chondrocyte like cells are better in security, and have a broad application prospect in the field of cartilage tissue engineering and regenerative medicine.

The invention discloses a method for converting in-vitro activated adipose-derived stem cells into chondroblasts. The method comprises the following steps of preparing a DMEM culture medium, wherein the DMEM culture medium is prepared from 1% of fetal calf serum, 10 ng / ml of TGF-beta, 100 ng / ml of BMP-6, 6.25 mu g / ml of insulin, 0.1 mu mol / L of dexamethasone, 6.25 mu g / ml of transferrin and 50 mumol / L of ascorbyl phosphate; culturing the adipose-derived stem cells formed into micelles in the DMEM culture medium; and injecting the adipose-derived stem cells cultured for 7-9 days into an articular cavity and converting the adipose-derived stem cells into chondroblasts. The method has the advantages of time saving, simple process control and low rejection of the generated chondroblasts.

The invention provides a method for inducing differentiation of adipose derived stem cells into chondrocyte. The method comprises the steps of preparing adipose derived stem cell-sodium alginate suspension, preparing adipose derived stem cell-calcium alginate microbeads, and performing induced culture. According to the method for inducing differentiation of adipose derived stem cells into chondrocyte, an incomplete cartilage forming induced culture solution containing PRP and transforming growth factor-beta superfamily members (TGF-[beta]1, IGF-1 and BMP-6) is utilized, and PRP and transforming growth factor-beta superfamily members are in united use, so that synergistic reactions are achieved, Sox-9 gene expression can be raised, differentiation of induced adipose derived stem cells intohypertrophy phenotypes is restrained, cell proliferation is promoted, and induced differentiation efficiency is improved. The proliferation vitality of the chondrocyte obtained through induced differentiation for 13-15 days by the method disclosed by the invention is 3.4 times of that of a control group A, 2.2 times of that of an experimental group B, and 1.95 times of that of an experimental group C.

The invention discloses a disposal method for a tissue-engineered graft, comprising the following steps: (a) placing a bone graft or a cartilage graft and osteogenesis induced liquid or chondroblast induced liquid in a container for 2 hours to 14 days under 37 plus or minus 2 DEG C; (b) taking out of the bone graft or the cartilage graft from the container as the preparation material for transplant operation. The method can preserve the graft in a short term but brings no damage to the graft and keep good physical performance while the cost is low and the operation is easy.

The invention relates to a preparation method and application of polymer coating for long-term in-vitro culture of human fat stem cells; the preparation method comprises: treating a cover glass, preparing the polymer coating, carrying out long-term culture of human fat stem cells on the polymer coating, inducing differentiation of the human fat stem cells to lipoblasts, osteoblasts and chondroblasts, and carrying out characterizing three types of induced adult cells by staining; monomers include hydroxyethyl methylacrylate A, methacryloyloxyethyl trimethyl ammonium chloride solution E, cyclohexyl methacrylate L, and 2-diethylaminoethyl methacrylate K; the polymer coating is from EK1L1, E3K1L2 or E3A1L2, wherein the numbers refer to the mass ratios of the monomers. The preparation method and application of the polymer coating for long-term in-vitro culture of human fat stem cells have the advantages that the polymer coating based on acrylate / acrylamide has good optical clarity and biocompatibility, benefiting cell culture and observation; the polymer coating can promote the growth of human fat stem cells to maintain their reproducibility, and may also maintain stem cell characteristics of the human fat stem cells.

The invention relates to a method for differentiating SMSCs to chondrocytes through in-vitro adenovirus mediated BMP-2 / 7 gene coexpression induction. The method comprises the following steps: separating and culturing SMSCs; constructing a pAdTrack-CMV-BMP2-IRES-BMP7 double gene recombinant vector; transferring the fifth-generation SMSCs through utilizing the pAdTrack-CMV-BMP2-IRES-BMP7 double gene recombinant vector; amplifying the recombinant viruses obtained after the transfection; adding the recombinant viruses to a chondroblast induction culture solution for induction; and culturing for above 10 days to obtain fibrochondrocytes. The method which adopts the adenovirus mediated BMP-2 / 7 gene coexpression vector to transfer SMSCs has the advantages of high transgenic efficiency, instant expression and high safety, and allows the SMSCs to be differentiated to the chondrocytes through the in-vitro induction.