Cell culture solution for enhancing cartilage differentiation induction, method and application

A technology of cell culture and cartilage, applied in the direction of cell culture active agents, bone/connective tissue cells, biochemical equipment and methods, etc., can solve the problems of poor function and low induction rate

Pending Publication Date: 2020-10-27
北京中卫医正科技有限公司
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Problems solved by technology

[0005] Aiming at the defects of low induction rate and poor function in the prior art of transforming chondrocytes from induced mesenchymal stem cells, the present invent

Method used

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  • Cell culture solution for enhancing cartilage differentiation induction, method and application
  • Cell culture solution for enhancing cartilage differentiation induction, method and application
  • Cell culture solution for enhancing cartilage differentiation induction, method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0032] 1. Preparation of placental blood platelet lysate plasma (PBPL)

[0033] 1. Take 200mL placental blood into the blood collection bag, and use 2000UI sodium heparin as an anticoagulant. Draw placental blood and pack into 50ml sterile centrifuge tubes. Centrifuge at 2000rpm at 4°C for 10-15min. Collect the supernatant.

[0034] 2. Transfer the supernatant collected in the previous step to a new 50ml centrifuge tube, centrifuge at 1500rpm for 10min, discard the upper 3 / 4 supernatant and the bottom red blood cells, and shake the remaining middle layer on a vortex shaker to mix Uniform, that is, placental blood platelet-rich plasma.

[0035] 3. The platelet-rich plasma collected in the previous step was freeze-thawed three times to prepare platelet lysate. That is, the platelet-rich plasma was placed at -80°C / 37°C and repeatedly frozen and thawed three times with an interval of 10 to 15 minutes.

[0036] 4. Centrifuge the plasma lysate collected in the previous step at ...

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PUM

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Abstract

The invention discloses a cell culture solution for enhancing cartilage differentiation induction, a method and an application. A blood platelet lysate is extracted and prepared from clinically wasteplacental blood, so that the source is wide, and the cost is low. Mesenchymal stem cells obtained by the method can stably grow in an adherent manner, characteristics are similar to those of cells obtained by a conventional culture method, and the cells are in a typical fusiform vortex shape under a microscope; and compared with the conventional culture method, the method has the advantages that expression of cartilage differentiation marker genes SOX9 and COL2A1 in the cells can be effectively activated, the number and strength of formed cartilages after in-vitro induction are obviously improved, and the higher chondroblast differentiation capacity is shown.

Description

technical field [0001] The invention belongs to the technical field of cartilage tissue engineering, and relates to a cell culture solution for enhancing and inducing cartilage differentiation, a method and an application. Background technique [0002] Cartilage damage is an important cause of osteoarthritis and joint dysfunction. The repair of articular cartilage defects has always been a difficult and hot topic in orthopedic research. The traditional articular cartilage protective treatment methods have great differences in treatment effects and low long-term satisfaction; the dysfunction caused by severe cartilage damage can only be treated through joint surgery. Replacement is a solution, which is expensive and painful, and some patients need to be replaced again. [0003] In recent years, the development of cartilage tissue engineering has provided people with more ideas for cartilage defect repair. Among them, umbilical cord mesenchymal stem cells (UC-MSCs), as the s...

Claims

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Application Information

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IPC IPC(8): C12N5/077A61L27/38
CPCA61L27/3834A61L27/3852A61L27/3895A61L2430/06A61L2430/40C12N5/0655C12N2500/84C12N2500/90C12N2501/999C12N2506/1392
Inventor 修冰水李帅民宋娅莉史秀珍刘世红
Owner 北京中卫医正科技有限公司
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