24 results about "Cartilage damage" patented technology

Disclosed is use of a Histatin-1 polypeptide in promotion of cartilage regeneration or repair. By preparing a combined reagent containing a Histatin-1 polypeptide, a biological scaffold material and the like, mainly a linear Hst1 is loaded on gelatin methacrylate so as to be used for repairing cartilage damage or promoting cartilage growth; meanwhile, disclosed is use of a Histatin-1 polypeptide in promotion of the expression of one or more of bone differentiation indexes, including a collagen fiber, glycosaminoglycan, type II collagen and aggrecan.

The present invention discloses the use of a benzenesulfonamide compound in the preparation of a medicament for preventing and / or treating osteoarthritis. This invention proposes for the first time the use of a benzenesulfonamide compound in a medicament for preventing and / or treating osteoarthritis. Experiments have shown that administration of a benzenesulfonamide compound can significantly improve joint swelling and wear in osteoarthritis mice; at the same time, the benzenesulfonamide compound can significantly reduce cartilage damage and inhibit the progression of joint fibrosis in osteoarthritis mice; in addition, the benzenesulfonamide compound significantly inhibits the expression of inflammation-related markers in the serum of osteoarthritis model mice. The above experiments demonstrate that benzenesulfonamide compounds can improve osteoarthritis and are of great significance for the future development of drugs and the prevention and treatment of such diseases.

The invention discloses a parasitic loranthus exosome-like nano vesicle and application thereof in preparation of a medicine for treating temporomandibular joint osteoarthritis. The taxillus sutchuenensis exosome-like nano-vesicles are prepared by combining a differential ultracentrifugation method with an ultrafiltration method. Researches show that the taxillus sutchuenensis exosome-like nano-vesicles can treat temporomandibular joint osteoarthritis through the following aspects: 1) an anti-inflammatory function: effectively reducing expression of inflammatory factors; 2) a cartilage degeneration inhibition function: blocking a cartilage matrix degradation pathway, and slowing down cartilage tissue damage; the taxillus sutchuenensis exosome-like nano-vesicles have the advantages that the taxillus sutchuenensis exosome-like nano-vesicles have obvious protection and repair effects on relieving temporal-mandibular joint osteoarthritis related bone and cartilage damage, and accordingly the taxillus sutchuenensis exosome-like nano-vesicles have good application prospects.

This invention relates to the field of biomedical materials, providing non-denatured type II collagen ultrafine powder with cartilage tissue-targeted growth and structural repair functions, its bioactivity preservation preparation method, and its application in joints. The ultrafine powder has an average particle size of 0.5-50 μm, a triple helix structure integrity rate of not less than 85%, a thermal denaturation temperature of 38-48℃, and retains chondrocyte binding domains CB10, CB11, and MMP cleavage sites. The preparation method includes raw material pretreatment, time-limited enzymatic hydrolysis, graded purification, and ultra-low temperature ball milling. The product can be used for the repair and treatment of cartilage degenerative diseases such as osteoarthritis and cartilage damage.

The application provides a composition containing curcuma and type II collagen-containing cartilage powder and application thereof in joint maintenance, and relates to the field of food technology. The application provides a composition containing milk mineral salt, curcuma, type II collagen-containing cartilage powder, enzymatic bone powder, N-acetyl glucosamine and black pepper extract, which can significantly repair joint damage, significantly reduce cartilage damage indexes such as CTX-II and COMP, and can be used for preparing a joint maintenance medicine.

The present invention provides an integrated design and manufacturing technology for a bone cartilage biomimetic gradient scaffold. The invention includes a functionally graded integrated bone cartilage biomimetic gradient scaffold, a transition structure for achieving graded transition between hard bone and cartilage tissue, and a biomimetic cartilage formation and post-processing method. The integrated bone cartilage biomimetic gradient scaffold is a porous interconnected ceramic structure that can be manufactured through integrated additive manufacturing. The transition structure is a special structure for firmly connecting hard bone and cartilage. The biomimetic cartilage formation and post-processing method involves performing special treatment on the 3D-printed bone scaffold to form biomimetic cartilage, thereby improving the strength of the cartilage layer and preventing cartilage damage caused by joint friction. The bone cartilage biomimetic scaffold structure proposed by the present invention has effective functional grading to mimic natural bone cartilage tissue, which is beneficial for preventing stress shielding and can be manufactured in one piece using 3D additive manufacturing technology.

The invention relates to the technical field of functional food, in particular to an electrolyte composition beneficial to joint injury repair after exercise and a preparation method of the electrolyte composition. The electrolyte composition is mainly prepared from the following raw materials in parts by mass: 0.1 to 2 percent of N-acetylglucosamine, 0.05 to 0.5 percent of cartilage extract, 0.05 to 0.5 percent of enzymolysis bone meal, 0.01 to 0.05 percent of milk mineral salt, 0.1 to 1 percent of collagen peptide, 0.01 to 0.3 percent of vitamin and 0.03 to 0.3 percent of electrolyte. The nano hydrated milk mineral salt is adopted, the absorption efficiency of elements such as calcium and phosphorus in a body is improved, and the electrolyte composition is prepared by combining components such as N-acetylglucosamine and the cartilage extract, so that joint inflammation and cartilage injury caused by exercise are effectively improved while water and electrolyte are supplemented, and the activity of joints and exercises is improved. The electrolyte composition is good in taste, high in stability and suitable for joint protection and nutrition supplement after exercise.

The invention discloses a nano polymer for specific targeted degradation of COX-2, a preparation method and application, and belongs to the technical field of biological medicines. The nano polymer comprises polyethylene glycol methacrylate with ROS responsiveness, a functional monomer for targeted degradation of COX-2, and a ligand structure monomer for recruiting E3 ligase. The invention proves that compared with a single COX-2 inhibitor, the prepared PKP-NP nano polymer has more excellent treatment performance. The problem of hydrophobicity of a traditional PROTAC medicine is solved, COX-2 protein is specifically and continuously degraded, cartilage cell apoptosis is inhibited, and cartilage tissue damage is repaired; the composition can accurately gather in an articular cavity by virtue of inflammation microenvironment response, prolong in-vivo retention time, relieve joint inflammation and repair cartilage injury, and has no obvious side effect. An efficient, safe and lasting scheme is provided for osteoarthritis treatment, and a new idea for clinical treatment is developed.

The present invention relates to a composition for treating osteoarthritis, the composition containing an herbal medicine extract as an active ingredient. It was found that an herbal medicine complex extract according to the present invention improves an arthritis index and increases bone mineral mass and bone density in an osteoarthritis animal model. In addition, it was found that the herbal medicine complex extract prevents cartilage damage, thereby increasing the thickness of cartilage, and regulates the expression of tissue regeneration factors and inflammatory cytokines.

The invention discloses application of NAC in preparation of a medicine for treating temporomandibular joint disorder, and particularly discloses application of the medicine in effectively improving TMD joint tissue oxidation resistance, inhibiting inflammation level, promoting extracellular matrix secretion and inhibiting iron (ferrous) ions and lipid peroxides so as to improve cartilage injury, relieve pain in a condylar process region and improve osteoarthritis. As a clinical common medicine, NAC is low in cost, high in availability, excellent in cell and tissue safety and high in practicability. The medicine can be safely applied to TMD treatment by means of condyle local injection or condyle region skin application (microneedle) and the like, has important significance in clinical medication of TMD, and has good medication value and application prospect.

The invention relates to the technical field of personal health risk assessment, and particularly provides an ultrasonic image processing method for gout articular cartilage injury grading, which comprises a double sign degree acquisition module, a first screening module, a confidence coefficient acquisition module and a second screening module, the method comprises the following steps: determining the double-track sign degree of multiple frames of initial ultrasonic images of gout articular cartilage at different angles, screening to obtain intermediate ultrasonic images, and determining the cartilage injury assessment confidence of each target ultrasonic image set when the intermediate ultrasonic image with the maximum elastic modulus mean value of the double-track sign area is used for target ultrasonic image set division each time, so as to determine the cartilage injury assessment confidence of each target ultrasonic image set. And the target ultrasonic image set corresponding to the maximum cartilage injury evaluation confidence is taken as the final ultrasonic image, so that the accuracy and reliability of ultrasonic image screening are improved, ultrasonic images with low reference value or even without reference value are prevented from participating in model training, and the reliability of a gout articular cartilage injury grading model is improved.

The present application relates to the technical field of personal health risk assessment, and specifically provides an ultrasonic image processing method for gout joint cartilage injury grading, comprising a double-track sign degree acquisition module, a first screening module, a confidence degree acquisition module and a second screening module, the double-track sign degree of multiple frames of initial ultrasonic images of different angles of gout joint cartilage is determined, and intermediate ultrasonic images are obtained through screening, in each time, the maximum intermediate ultrasonic image is taken as the target ultrasonic image set, the cartilage injury evaluation confidence of each target ultrasonic image set is determined, the target ultrasonic image set corresponding to the maximum cartilage injury evaluation confidence is taken as the final ultrasonic image, the accuracy and reliability of ultrasonic image screening are improved, and the ultrasonic images with little reference value or even no reference value are avoided to participate in model training, and the reliability of the gout joint cartilage injury grading model is improved.

The application relates to a preparation method of a cartilage repair material, a product and application thereof, in particular to a preparation method of a hydrogel for repairing a cartilage microenvironment. The application has the advantages that calcium phosphate particles are induced to deposit to form an organic-inorganic composite structure through a three-dimensional structure of an organic hydrogel, and the rigidity of the hydrogel is improved. MgH2@ZIF-8 NPs contained in the hydrogel can release hydrogen under an acidic pH condition, the inflammatory microenvironment of cartilage damage is adjusted, and the effect of immunorepair is achieved.

The invention discloses an external-use compound formula for assisting skeletal development and cartilage health of teenagers, and belongs to the technical field of external-use health-care preparations. According to the formula, yak bone marrow peptide, glucosamine hydrochloride, chondroitin sulfate and bone collagen peptide serve as core active ingredients, rose hip oil is matched to serve as a transdermal penetration enhancing component, transdermal absorption of the active ingredients is achieved through reasonable matching, osteoblast metabolism of teenagers (6-18 years old) can be promoted in a targeted mode, the bone mineral density can be increased, and the effect of preventing and treating osteoporosis is achieved. Meanwhile, cartilage matrix synthesis is strengthened, cartilage injury is reduced, and the problem that external health care products are deficient in the teenager skeletal cartilage development stage is solved. According to the technical verification of the Zhejiang traditional Chinese medicine university medicine college modern medicine research center, the formula is high in safety and excellent in transdermal efficiency, adapts to teenager skin barrier characteristics, and has remarkable application value in the fields of teenager skeletal development and cartilage health maintenance.

The invention belongs to the field of biomedicine, and particularly relates to application of BSP-MA / HA-NB hydrogel to preparation of medicine for treating bone defects, cartilage injuries or articular cartilage degenerative diseases. HA-NB (o-nitrosobenzaldehyde modified hyaluronic acid) is introduced into BSP-MA (methylacryloylated bletilla striata polysaccharide) to prepare the BSP-MA / HA-NB hydrogel, the BSP-MA / HA-NB hydrogel shows an obvious synergistic effect in the aspect of promoting cartilage and subchondral bone regeneration, and meanwhile, the BSP-MA / HA-NB hydrogel can be used for promoting cartilage and subchondral bone regeneration by inhibiting activation of M1 macrophages and promoting increase of M2 macrophages, so that the cartilage and subchondral bone regeneration can be promoted, and the cartilage and subchondral bone regeneration can be promoted. Therefore, inflammatory response is reduced, tissue repair is promoted, and the compound can be used for treating cartilage injury and / or articular cartilage degenerative diseases and has a wide application prospect.

The invention belongs to application of a substance for regulating protein FGF7 and a medicine for treating or / and relieving osteoarthritis in the field of genetic engineering. The technical problem to be solved by the invention is to provide new application of the protein FGF7 in treating or / and relieving osteoarthritis. In order to solve the technical problem, the invention provides application of FGF7 in preparation of products for treating or / and relieving osteoarthritis, and the amino acid sequence of the protein FGF7 is SEQ ID NO: 1. Research finds that after FGF7 expression in mouse joints is inhibited, abnormal bone formation can be relieved, cartilage injury repair can be promoted, and a protection effect on the joints is achieved.

The invention discloses an application of a pyrimidine compound in preparation of a medicine for preventing and / or treating osteoarthritis. The invention provides the application of the pyrimidine compound in the medicine for preventing and / or treating osteoarthritis for the first time. Experiments show that the compound can relieve pain in the joint area of a mouse, meanwhile, cartilage injury of the mouse with osteoarthritis can be obviously relieved, and the joint fibrosis process is inhibited; in addition, the pyrimidine compound can obviously inhibit the formation of osteophyte in a joint area; in addition, the compound significantly inhibits expression of inflammation-related markers in osteoarthritis model mouse joint regions and serum. The experiment shows that the pyrimidine compound can achieve the purpose of improving osteoarthritis, and has very important significance on drug development and prevention and treatment of the diseases in the future.

This invention belongs to the biomedical field, specifically relating to the use of BSP-MA / HA-NB hydrogel in the preparation of drugs for treating bone defects, cartilage damage, or degenerative diseases of articular cartilage. This invention introduces HA-NB (hyaluronic acid modified with o-nitrosobenzaldehyde) into BSP-MA (methacrylated Bletilla striata polysaccharide) to prepare BSP-MA / HA-NB hydrogel, which exhibits a significant synergistic effect in promoting cartilage and subchondral bone regeneration. Simultaneously, it can reduce inflammatory responses and promote tissue repair by inhibiting the activation of M1 macrophages and promoting the increase of M2 macrophages. It can be used to treat cartilage damage and / or degenerative diseases of articular cartilage, and has broad application prospects.

The application discloses a stem cell medicine composition for improving arthritic microenvironment, protecting cartilage and inhibiting synovial fibrosis, and a preparation method and application thereof, and belongs to the technical field of cell biology medicine. Active ingredients of the application are P6-P10 generation human umbilical cord mesenchymal stem cells which are directionally domesticated by osteoarticular compound pathological microenvironment, wherein the positive rate of the stem cells transmitted to P15 generation is less than or equal to 7%, and the comprehensive repair activity retention rate is greater than or equal to 93%; the final concentration of cells in the preparation is 1*10^6-2*10^6 pieces / mL, the pH is stable at 7.2-7.4, and injection carriers and low-temperature protection auxiliaries are matched. The stem cells are endowed with joint target repair ability by pathological microenvironment domestication, and multiple target points are synchronously intervened in arthritic inflammation, cartilage damage and synovial fibrosis, so that the industry defects of ordinary stem cells, such as weak targeting, unstable activity and large batch difference, are solved; the preparation has high standardization, can be prepared into liquid injection and freeze-drying storage and transportation dosage forms, is suitable for GMP large-scale production, clinical trials and new drug declaration, and has high clinical conversion value.

The invention provides methods and compositions for the treatment of cartilage damage or arthritis.

This invention discloses a traditional Chinese medicine composition for the prevention and / or treatment of osteoarthritis and its application. It comprises, by weight, 10-30 parts of *Achyranthes bidentata*, 10-30 parts of *Pueraria lobata*, 5-20 parts of *Alpinia oxyphylla*, 5-20 parts of *Curcuma longa*, and 5-20 parts of *Cornus officinalis*. Scientific research has confirmed that the aforementioned traditional Chinese medicine composition of *Achyranthes bidentata*, *Pueraria lobata*, *Alpinia oxyphylla*, *Curcuma longa*, and *Cornus officinalis* can alleviate pain and inflammation caused by osteoarthritis, reduce cartilage damage caused by joint injury, and prevent the occurrence and development of osteoarthritis. It has both preventive and therapeutic effects on osteoarthritis, and the traditional Chinese medicine composition containing *Achyranthes bidentata*, *Pueraria lobata*, etc., can be developed into a drug for the prevention and / or treatment of osteoarthritis.

A method for optimizing osteoarthritis (OA) treatment by promoting chondrocyte cell proliferation and migration using thrombomodulin (TM) or soluble TM. This method involves blocking interleukin 1β (IL-1β)-mediated signaling to prevent the loss of bone integrity and maintain knee joint function. Additionally, the administration of miR-up-TM enhances the expression of TM protein, thereby protecting against cartilage damage and potentially preventing cartilage-related diseases.

This invention discloses a *Taxillus chinensis* exosome-like nanovesicle and its application in the preparation of a therapeutic drug for temporomandibular joint osteoarthritis. The *Taxillus chinensis* exosome-like nanovesicle is prepared by differential ultracentrifugation combined with ultrafiltration. This invention demonstrates that *Taxillus chinensis* exosome-like nanovesicle can treat temporomandibular joint osteoarthritis through the following aspects: 1) Anti-inflammatory function: effectively reducing the expression of inflammatory factors. 2) Inhibition of cartilage degeneration function: blocking the degradation pathway of cartilage matrix and slowing down cartilage tissue damage. 3) Promotion of cartilage regeneration function: activating the synthetic activity of articular chondrocytes and enhancing cartilage repair capacity. 4) Restoration of condylar bone microstructure. The *Taxillus chinensis* exosome-like nanovesicle of this invention exhibits significant protective and repairing effects in alleviating bone and cartilage damage associated with temporomandibular joint osteoarthritis, and has promising application prospects.

The invention provides viscoelastic hydrogel microspheres with adjustable stress relaxation performance as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. According to the invention, the idea of a'cell shock absorption 'strategy is realized, and the viscoelastic hydrogel microspheres with adjustable stress relaxation performance are successfully constructed. Chemical bonds in a hydrogel network are regulated and controlled through oxidation and hydrazine coupling reaction, and the hydrogel microspheres capable of absorbing impact and relieving cartilage cell ER stress induced by mechanical stress are prepared. The preparation method comprises the following steps: encapsulating cationic liposome modified with cartilage targeting peptide Wyrgrl and tauroursodeoxycholic acid (TUDCA) in a microsphere; the microsphere system not only can obviously dissipate impact force in a joint cavity and reduce vibration and pressure conduction, but also can realize continuous release of TUDCA, so that ER stress is relieved, and OA progress is delayed. According to the microsphere strategy, dual protection on cartilage injury can be realized by reducing mechanical stress and relieving ER stress, and the microsphere shows a good application prospect in the aspect of delaying osteoarthritis.