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Tissue- engineered cartilage graftimplant and preparation method thereof

A technique of tissue engineering and grafts, applied in the field of tissue engineered cartilage grafts and their preparation, and repair materials for cartilage defect repair, can solve the problems that cartilage defect repair has not been effectively solved, and an ideal has not been produced. , to achieve the effect of improving biomechanical properties, facilitating adhesion growth and vascularization, and improving healing speed and effect

Inactive Publication Date: 2014-01-08
THE SECOND PEOPLES HOSPITAL OF SHENZHEN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] For a long time, cartilage defect repair has been an important research topic in the field of biomaterials and medicine. However, the repair of large-scale cartilage defects caused by trauma, infection and tumor resection has not been effectively solved in clinical practice so far.
Cartilage transplantation as an effective cartilage defect repair method has been recognized by scholars, but because an ideal artificial material has not yet been produced as a substitute for cartilage transplantation, the study of cartilage defect repair materials has become a hot topic in the past century one

Method used

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  • Tissue- engineered cartilage graftimplant and preparation method thereof
  • Tissue- engineered cartilage graftimplant and preparation method thereof
  • Tissue- engineered cartilage graftimplant and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1: Preparation of Nano-HA:

[0050] Hydroxyapatite was synthesized by sol-flocculation method. Preparation method: chemically synthesize the aqueous solution of calcium nitrate and ammonium phosphate, add a certain amount of ammonia water during the synthesis process, adjust the pH value of the solution to 8-13, add a dispersant, select a suitable stirrer, adjust the speed of the stirrer and stir Time to make it precipitate completely, then wash and filter, dry the precipitate at 80-120°C, and sinter at 600-800°C for 2-3 hours to obtain a powder with a particle size of less than 100nm that is similar to the composition of human bone tissue Nano-hydroxyapatite powder.

Embodiment 2

[0051] Embodiment 2, Preparation of PLLA

[0052] 1. Preparation of lactide: Take lactic acid and ZnO powder, gradually increase the temperature to dehydrate the lactic acid into lactide; then heat up and vacuumize to evaporate the light yellow lactide monomer, then use ethyl acetate as solvent to repeatedly wash and re- Purification by crystallization, suction filtration, and vacuum drying yielded colorless flaky pure lactide crystals.

[0053] 2. Synthesis of PLLA: pure lactide monomer, add stannous octoate initiator, vacuum dry at room temperature for a certain period of time, soak in a silicone oil bath for 3-5min, seal the reaction bottle, and shake it repeatedly when the temperature rises until the monomer just melts The reaction bottle was mixed evenly, and then timed to polymerize for a certain period of time to take out the reaction bottle.

[0054] 3. Purification of PLLA: Add dichloromethane to the crude PLLA to dissolve it completely, filter, add methanol to the...

Embodiment 3

[0057] Example 3: Preparation of tissue-engineered cartilage grafts:

[0058] A tissue engineered cartilage graft, comprising a scaffold material and seed cells adhered to the scaffold material, the seed cells are chondrocytes induced and differentiated by BMSCs through TGF-β1; the preparation method of the tissue engineered cartilage graft as follows:

[0059] 1. Preparation of Nano-HA / PLLA cartilage scaffold material

[0060] (1), Preparation of Nano-HA / PLLA cartilage scaffold material:

[0061] (a), dissolving Nano-HA and PLLA in an organic solvent in a mass ratio of 1:4, and mixing uniformly to obtain a prepared biological material;

[0062] (b) Form parallel ice lines with intervals by RIPF process, the filling speed of RIPF process is 20mm / s, and the filling speed determines the interval between parallel ice lines;

[0063] (c), filling the prepared biological material into the interval between the ice lines, and scraping the surface filled with the biological mate...

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Abstract

The invention relates to a tissue tissue-engineered cartilage graftimplant and a preparation method thereof and belongs to the technical field of induced differentiation carried out on bone marrow mesenchymal stem cells (BMSCs) by utilizing a bioactive inducing factor to form a cartilage cell chondroblast composite scaffold material and so as to construct a tissue tissue-engineered cartilage by utilizing a biological activity inducing factor in biomedicine tissue engineering. The tissue tissue-engineered cartilage graftimplant is prepared by adopting the method comprising the following steps: (1) preparing a Nano-HA / PLLA (hyaluronic aciddroxyapatite / poly left L-lactic acid) cartilage scaffold material; (2) carrying out coculture on BMSCs and the Nano-HA / PLLA cartilage scaffold material, and carrying out induced differentiation on BMSCs to form cartilage cells by adopting a cartilage formation inducing solution, so that the tissue tissue-engineered cartilage graftimplant is obtained. The tissue tissue-engineered cartilage graftimplant improves flexibility and biodegradability of the cartilage scaffold material, improves biomechanical property and is more beneficial to adhesion, growth and vascularization of bone cells; an animal experiment proves that the tissue tissue-engineered cartilage graftimplant has a good cartilage defect repairing function.

Description

technical field [0001] The invention belongs to the technical field of constructing tissue engineered cartilage by using bioactive inducing factors to induce differentiation of BMSCs chondrocyte composite scaffold materials in biomedical tissue engineering, and relates to a repair material for cartilage defect repair, in particular to a tissue engineered Cartilage grafts and methods for their preparation. Background technique [0002] For a long time, cartilage defect repair has been an important research topic in the field of biomaterials and medicine. However, so far, the repair of large-scale cartilage defects caused by trauma, infection and tumor resection has not been effectively resolved clinically. Cartilage transplantation as an effective cartilage defect repair method has been recognized by scholars, but because an ideal artificial material has not yet been produced as a substitute for cartilage transplantation, the study of cartilage defect repair materials has bec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/46A61L27/12A61L27/18A61L27/38A61L27/56C12N5/0775
Inventor 王大平黄江鸿熊建义朱伟民刘建全尤微段莉张巨峰陈洁琳
Owner THE SECOND PEOPLES HOSPITAL OF SHENZHEN
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