The invention discloses a novel human-like collagen haemostatic dressing which has good biocompatibility, can be absorbed and is rich in calcium, zinc, copper and iron, and discloses a preparation method of the novel human-like collagen haemostatic dressing. The preparation method comprises the following steps: by taking modified protein obtained by chelating humanized human-like collagen prepared by high-density fermentation with calcium, zinc, copper and iron ions respectively as a raw material as well as taking lysyloxidase existing in mammals as a cross-linking agent, carrying out low-temperature cross-linking reaction and freeze-drying processes to obtain the novel collagen haemostatic dressing which can be used for hemostasis of wounds and postoperative hemostasis. The preparation method disclosed by the invention has the advantages that the raw materials are easy to obtain, the cost is low, the synthetic process is simple and easy to realize, process amplification is easy, and the reproducibility is good; and the human-like collagen haemostatic dressing prepared by the preparation method does not have animal-derived virus hidden danger, has an obvious haemostatic effect, can be used for hemostasis of various wounds and postoperative hemostasis, and can be used for basic research and application of related fields, such as biomedical tissue engineering.