Preparation method for tissue engineering corium

A tissue engineering, dermis technology, applied in skin transplantation, medical science, prosthesis, etc., can solve the problems of not achieving epidermal reconstruction, slow vascularization, limited sources, etc.

Inactive Publication Date: 2005-12-21
陕西艾尔肤组织工程有限公司
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main disadvantages are: lack of viable fibroblasts, which may delay dermal reconstruction; collagen in acellular xenogenic dermal matrices may cause immune reactions; commercially available products only achieve dermal replacement, not epidermal reconstruction, and must be covered with autologous or cultured epidermis Cell sheets; Alloderm is derived from cadaver skin, limited source and risk of spreading viral diseases (especially AIDS)
But there are also disadvantages: a large number of fibroblasts are required to produce synthetic omentum dermal substitutes; it is difficult to change the thickness of the omentum, and the cost is high
Disadvantages are slow vascularization, poor anti-infection ability, and expensive
At present, due to various reasons, there is no ideal artificial leather available in China.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0012] Now in conjunction with experiment the present invention will be described in further detail:

[0013] 1. Acquisition and expanded culture of fibroblasts: Take the skin tissue after circumcision of newborns. It has been confirmed that the fibroblasts from this source have a low immune response and will not cause obvious rejection. After the obtained foreskins were disinfected with promethamine or alcohol, fibroblasts were cultured by enzyme separation method or tissue block method. After the cells were confluent, they were digested and passaged with enzymes, and expanded for culture. The specific process is:

[0014] Wash the sterilized material with 0.1M phosphate buffered saline (PBS) containing 1% penicillin / streptomycin, remove subcutaneous fat and muscle layer, cut the skin into strips, add protease (Dispase) 4U / ml, Digest at 37 °C for 60-120 min. Remove the epidermis, cut the dermal tissue into small pieces, add 625U / ml collagenase to digest at 37°C for 90-160 m...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the technical field of biomedical tissue engineering, and relates to a tissue engineering dermis and a preparation method thereof. The characteristics of the method include the acquisition and expanded culture of skin fibroblasts, the preparation of extracellular matrix complexes, the preparation of biological scaffold materials, the preparation of medium, the preparation of tissue engineered dermis and three-dimensional culture. The prepared tissue engineered dermis is constructed by compounding skin fibroblasts on the surface of biological scaffold materials. The tissue engineered dermis prepared by the invention not only has certain elasticity and toughness, but also has short culture time and no obvious immune rejection reaction, which is similar to the structure of natural dermis. It will play a multi-faceted role in clinical treatment.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering of biological materials, and relates to a tissue engineering dermis and a preparation method thereof. Background technique [0002] In recent years, in the fields of medical cosmetology, trauma, burn surgery, etc., with the requirements for functional recovery and aesthetic considerations, it is far from enough to achieve epithelialization of some deep skin defects, and efforts must be made to explore and apply more Reasonable method, so that the skin graft area can finally achieve the same or similar results as full-thickness skin grafting. Dermal substitutes play an important role in the skin reconstruction process. They can enhance skin elasticity, flexibility and mechanical wear resistance after wound healing, reduce scar hyperplasia, control contractures, improve the success rate of epidermal grafting and improve the quality of healing. Live dermal substitutes containing fibroblas...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): A61F2/10A61L27/00A61L27/60
Inventor 金岩张勇杰聂鑫王新文董蕊
Owner 陕西艾尔肤组织工程有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap