24 results about "Dermal fibroblast" patented technology

This invention provides a method for obtaining ingredients from Dulong Paris polyphylla (… Paris dulongensis A method for preparing parisyunnanoside G (PFE55) in H. Li & Kurita and its application in cosmetics and pharmaceuticals. This research belongs to the field of cosmetics and pharmaceutical technology. PFE55 is used at extremely low concentrations (0.1... μ At a concentration of g / mL, it exhibits activity in promoting collagen secretion in adult dermal fibroblasts (HDFa) and is non-cytotoxic at this concentration, making it suitable for the preparation of anti-aging drugs or skincare products.

The present application belongs to the field of biomedical technology, and particularly relates to a cell-derived extracellular matrix and a preparation method and application of a cell culture plate coated with the cell-derived extracellular matrix. The present application cultures human astrocytes in a cell culture plate, and makes the human astrocytes secrete extracellular matrix. After the human astrocytes are added into a cell lysate for cell removal treatment, nucleic acid fragmentation treatment is performed to obtain the cell-derived extracellular matrix. The present application has relatively simple operation, short time period, and can realize batch production of extracellular matrix without external virus carrying and stable components. The obtained cell-derived extracellular matrix is resistant to storage, has good stability, is highly similar to a natural brain environment when coated on a cell culture plate, has good biocompatibility with cells, can be applied to tissue repair and regeneration as an ideal biomedical material, can regulate the progress and efficiency of human dermal fibroblast HDF transdifferentiation into neurons, and can obtain in-vitro survival neurons.

This invention proposes a nucleic acid aptamer that specifically binds to human senescent dermal fibroblasts (HDFs), along with its screening, identification, and application. Specific aptamers exhibiting high targeting specificity to human senescent HDFs were screened using Cell-SELEX technology. The three selected candidate aptamers showed binding rates of 57.5%, 86.2%, and 50.7% to senescent HDF cells within the same timeframe, respectively, demonstrating high specificity and targeted binding ability. Furthermore, the aptamer possesses potential for detecting, imaging, and regulating the function of senescent cells, successfully providing a new direction for targeted anti-aging interventions and therapeutic applications.

The application provides application of a carrot-derived extracellular vesicle-like particle in preparation of a skin wound repair product, and belongs to the technical field of biological medicine. The extracellular vesicle-like particle has good water dispersibility and low-temperature storage stability; can be taken by dermal fibroblasts, vascular endothelial cells and the like, can reduce cell apoptosis, improve cell activity, promote proliferation, migration and blood vessel formation related functions under high-sugar high-fat and the like damage conditions, thereby synergistically improving the functions of dermal fibroblasts and vascular endothelial cells, and has certain antibacterial capacity, thereby being beneficial to skin wound healing, and providing a scheme and a clinical conversion idea for wound treatment.

Identification of dermal fibroblast subpopulations. [Solution] The object of the present invention is a method for identifying dermal fibroblasts in vitro as papillary fibroblasts, reticular fibroblasts or dermal-subcutaneous junction fibroblasts (FJDH), the method comprising a step of measuring the levels of expression products of UCP2 and FGF9, and optionally at least one gene selected from the group consisting of the COL11A1 gene and the ACAN gene, and the level of expression product of the KLF9 gene.

The present application relates to the use of a substance that regulates BCL11A gene and / or its encoded product in the preparation of a drug for reversing skin photoaging, wherein the skin photoaging includes skin cell aging caused by sunlight, light, etc., and the skin cells include keratinocytes, dermal fibroblasts, melanocytes. The regulation is to up-regulate the expression of BCL11A gene and / or promote the production of its encoded product. Typical clinical manifestations of the skin photoaging include wrinkles, dry skin, loose skin, dilated capillaries, uneven skin pigmentation, and skin itching. The substance that regulates BCL11A gene and / or its encoded product includes a substance that regulates BCL11A gene DNA molecules and a substance that regulates the expression of BCL11A translation product protein molecules. The reversal of skin photoaging is manifested in reducing the proportion of cells positive for aging-related beta-galactosidase staining in skin cells and reducing the expression of aging-related protein p16 and DNA damage protein gammaH2AX.

Application of mulberry bark effective part in whitening and anti-UVA damage and preparation method thereof, the effective part is that mulberry bark is extracted with ethanol, the obtained extract is concentrated under reduced pressure, then dissolved with ethanol, then adsorbed with macroporous resin, eluted with different concentrations of ethanol, different concentrations of ethanol eluate is collected respectively, then concentrated under reduced pressure to obtain the effective part. α The protective effect of the effective part on UVA damaged human dermal fibroblasts (HDF- α ) is equivalent to that of the positive control vitamin C (100 μM). The effective part (0.3% emulsion) shows significant whitening effect in ultraviolet irradiation test population, which is equivalent to that of the positive control vitamin C (7% emulsion). The effective part of the application has good application prospect in developing whitening, anti-photo damage or double effect cosmetics.

The purpose of the present invention is to identify a dermal fibroblast aging subpopulation. The present invention has reclassified dermal fibroblasts using a single-cell analysis technique, and upon doing so, has discovered a novel subpopulation that has not previously been classified, on the basis of differences in the gene expression of dermal fibroblasts which were isolated from tissue. This subpopulation is contained in a large amount in the skin of an elderly subject, and is also contained in the skin of a young subject. The subpopulation is identified as an aging subpopulation associated with the aging of the skin.

The invention relates to application of Antarctic red in a skin conditioning agent for synergistically controlling oil and resisting wrinkles, and belongs to the technical field of biology, the Antarctic red has no toxicity to cells when the concentration is not higher than 250 g / mL, gene expression of I-type 5 alpha-reductase in sebaceous gland cells is inhibited in a targeted mode, and excessive synthesis of grease is reduced from the source; the gene expression level of I type and III type collagen in dermal fibroblasts is synchronously improved, so that the internal support structure of the skin is reconstructed, and the anti-wrinkle effect is realized. According to the Antarctic red disclosed by the invention, through the synergistic effect of'descending and ascending ', the Antarctic red is proved to be capable of simultaneously and accurately intervening an'oil-wrinkle' associated pathway, and the limitation of a single functional component is broken through.

Disclosed herein are topical compositions comprising Centella asiatica (Centella asiatica) extract and mandelic acid, wherein the composition has a pH of less than 5, and methods of using the compositions to reduce cellular senescence in the skin and treat dermatoporosis. The topical composition comprising Centella asiatica extract and mandelic acid is effective in reducing the number of senescent cells in mammalian dermis, reducing the senescence-associated secretory phenotype (SASP), downregulating at least one SASP-related gene, increasing the expression of at least one cell cycle-related gene in dermal fibroblasts, downregulating at least one gene associated with skin inflammation, and increasing fibroblast turnover.

The invention relates to the technical field of gel, in particular to gel based on an intelligent response type dynamic double-membrane hydration system and a preparation method and application of the gel. Tris (hydroxymethyl) aminomethane and carbomer cooperate to form a carbomer-pH-sensitive hydrogel matrix, the swelling degree of the carbomer-pH-sensitive hydrogel matrix is dynamically adjusted along with the change of the pH of a scar microenvironment, and the pH-sensitive hydrogel matrix has the advantages that the stability is high; a static sealing mode of a traditional silicone preparation is broken through, intelligent hydration management of different scar repairing stages (an acute stage and a mature stage) is achieved, an outer silicone isolating membrane formed by polydimethylsiloxane and an inner bioactive lipid membrane formed by composite plant active lipid achieve synergistic interaction, the hydration efficiency is improved by 70% or above, and the application range is wide. The nano transdermal carrier provided by the invention solves the problem of transdermal penetration of silicone and plant active ingredients, and targets to dermal fibroblasts, and clinical data shows that the scar softening rate is improved by more than 40% after the gel is used for 7 days, and the hyperpigmentation improvement rate is improved by more than 50% compared with that of a traditional product.

This invention discloses a method for predicting and validating the comprehensive skin toxicity of daily-use preservatives based on multi-pathway responses in keratinocyte-fibroblast co-culture, belonging to the technical field of daily chemical safety evaluation. This invention uses human keratinocytes (HaCaT) and human dermal fibroblasts (HSF) as dual targets, and assesses the mechanistic skin toxicity of preservatives through multiple endpoints including inflammation, barrier damage, cell senescence, and organelle damage, classifying them and guiding their formulation application. The method of this invention boasts high prediction accuracy, high sensitivity, high efficiency, and compliance with regulations. It overcomes the limitations of traditional methods that only focus on "cell viability," providing a panoramic analysis of the toxic action pathways of preservatives from three dimensions: gene expression (early signal), organelle damage (intermediate process), and population apoptosis (final outcome), avoiding false negatives caused by single endpoints and possessing broad application prospects.

This invention provides steroidal saponins such as Paris polyphylla saponin A, Paris polyphylla saponin B, Xuefengshan saponin A, Xuefengshan saponin B, and Xuefengshan saponin C, their preparation methods, and their applications in the cosmetics and pharmaceutical fields. It belongs to the field of cosmetics and pharmaceutical technology. Paris polyphylla saponin VII, protodiospin, protodiospin, protodiospin, Xuefengshan saponin B, Xuefengshan saponin C, and methyl protosaponin Pb are prepared at extremely low concentrations (0.1%). m At concentrations of M, it exhibits activity in promoting collagen secretion in adult dermal fibroblasts (HDFa), with an increase in collagen secretion exceeding 50%; furthermore, at this concentration, it is not cytotoxic and can be used to prepare anti-aging drugs or skincare products.

The invention belongs to the technical field of cosmetics, and particularly relates to cyclic peptide for promoting expression of human skin full-configuration protein and application of the cyclic peptide in cosmetics. The structure of the cyclic peptide is shown as a formula (I) or (II). The cyclopeptide provided by the invention can significantly up-regulate the expression levels of COL1A1, COL3A1, ELN and ITGB1 in human dermal fibroblasts and promote the synthesis of collagen, elastin and integrin, so that the skin elasticity and compactness can be improved, and the cyclopeptide has excellent stability and biological safety. The cyclic peptide disclosed by the invention can be widely applied to cosmetics for resisting aging, improving skin elasticity, tightening and repairing. (I) (II)

The invention discloses a method for evaluating senescence of dermal fibroblasts. The method comprises the following steps: measuring the assembly density of F-actin in dermal fibroblasts, and comparing with a contrast; if the assembly density is lower than that of the contrast, evaluating that the dermal fibroblasts are senescent cells or senescent cells; the control includes the assembly density of F-actin in dermal fibroblasts that are defined to be unaged. The evaluation method has the effect of screening out symptoms such as skin wrinkles, relaxation and droop caused by the change of the physical and chemical environment of the extracellular matrix.

The invention belongs to the technical field of bioengineering, and particularly relates to recombinant human IV-type collagen as well as a preparation method and application thereof. According to the application, a segment from natural IV type collagen is selected, expression in escherichia coli is improved by optimizing an auxiliary element, and meanwhile, it is verified that the recombinant human IV type collagen can remarkably promote proliferation of human skin fibroblasts, promote senescent human primary dermal fibroblasts to generate energy and recover cell viability, so that the recombinant human IV type collagen can be used for improving the human skin fibroblasts. The method has good application potential in preparation of cosmetics and skin care products in the aspects of substrate membrane stability maintaining, repairing and the like.

Traditional research focusing on protein synthesis has focused on the total amount of protein, and therefore requires components that either suppress the decrease in the amount of newly synthesized protein or promote its synthesis. [Solution] Focusing on the synthesis of new proteins, and utilizing a method for evaluating the amount of new protein synthesis in dermal fibroblasts and epidermal keratinocytes, neem leaf extract and kudzu extract are provided as inhibitors of the decrease in the amount of new protein synthesis or as promoters of new protein synthesis.

The present disclosure provides an engineered dermis comprising one or more layers of human dermal fibroblast compositions as well as scaffolds and methods of the same. The disclosure also provides compositions comprising one or more interwoven extracellular matrices, wherein the interwoven extracellular matrices have been decellularized as well as scaffolds and methods of the same.

This invention discloses a method for extracting lipid components from dermal adipocytes and its application, comprising the following steps: (1) extracting primary dermal fibroblasts from mice on day 0 of birth; (2) culturing the primary dermal fibroblasts in DMEM+++ medium until the cell adhesion density reaches 100%, then inducing adipogenic differentiation in vitro using adipogenic differentiation medium, and then maintaining the culture in maintenance medium until the cells are in an early adipogenic state, and separating the supernatant of the early differentiated cells; (3) extracting and separating the supernatant obtained in step (2) using the Matyash method to obtain the lipid components of the dermal adipocytes. This invention, by extracting and separating lipid and protein components from the supernatant of early differentiated adipocyte culture, obtains lipid components with significant immunomodulatory functions, which can significantly inhibit the inflammatory state of macrophages and the proliferation of keratinocytes.

Disclosed are bioprinted, three-dimensional, biological skin tissues comprising: a dermal layer comprising dermal fibroblasts; and an epidermal layer comprising keratinocytes, the epidermal layer in contact with the dermal layer to form the three-dimensional, engineered, biological skin tissue. Also disclosed are arrays of engineered skin tissues and methods of making engineered skin tissues.

The application discloses composite PCL microspheres and a preparation method and application thereof. The ratio of the shell thickness of the composite PCL microspheres to the core radius is 1:1-1:3, and the particle size of the composite PCL microspheres is 20-65 mu m. The PCL polycaprolactone microspheres can make the dermal fibroblasts continuously proliferate under low inflammation reaction, make the fibroblasts grow in order around the composite microspheres, reduce the overall inflammation level, continuously produce collagen and extracellular matrix, and achieve the regeneration effect.

The present application provides a kind of composition for preventing or eliminating striae gravidarum and its preparation method and application, belong to the field of cosmetics.The present application composition includes the following raw materials by mass fraction: Rana chensinensis skin elastic polypeptide 3-5 parts, soybean oligopeptide 2-4 parts, Ganoderma lucidum polysaccharide extract 2-3 parts, sea buckthorn fruit oil extract 1.5-2.5 parts, calendula total flavonoid extract 1-2 parts, marula oil 4-6 parts and acacia vera oil 3-5 parts.The present application can significantly promote human dermal fibroblast proliferation, improve I / III collagen and elastin synthesis, and efficiently inhibit MMP-9 activity;Human trials show that the present application cream can effectively improve skin elasticity, reduce the area of striae gravidarum, fade red striae and redness, and improve rough skin, and is mild and non-irritating, suitable for sensitive skin during pregnancy and postpartum, with dual effects of prevention and repair.

The invention provides a traditional Chinese medicine composition for maintaining beauty and keeping young and application of the traditional Chinese medicine composition in health-care products, and relates to the technical field of traditional Chinese medicine, the traditional Chinese medicine composition is prepared from citron, seville orange flower, emblic leafflower fruit, perilla fruit, roxburgh rose, fresh reed rhizome, hyacinth bean flower, coix seed, rose, lophatherum gracile, fructus cannabis and radish seed. The traditional Chinese medicine composition disclosed by the invention has definite beauty maintaining and young keeping effects; antioxidant activity experiments show that the composition can effectively remove DPPH and ABTS < + > free radicals; cell experiments show that the composition can promote the synthesis of type I collagen, enhance skin matrix components and improve skin elasticity and compactness, and has no significant influence on the activity of human dermal fibroblasts (HDF). The traditional Chinese medicine composition disclosed by the invention can be widely applied to beauty-maintaining and young-keeping health-care products such as health-care wine, tea substitute drinks and the like, provides a scheme with traditional advantages and modern scientific basis for the field, and assists the innovative application of traditional Chinese medicines in the field of large health.

This invention discloses a novel application of walnut bark extract. The invention employs a complex enzymatic hydrolysis combined with gradient solvent extraction, supplemented by macroporous resin purification technology, to obtain a walnut bark extract enriched with polyphenolic compounds. It has been demonstrated that this extract can increase the elastin content in human dermal fibroblasts, inhibit the expression of various types of matrix metalloproteinases, and has the effects of improving skin firmness and anti-photoaging. Therefore, it can be applied in the preparation of cosmetics with firming and anti-photoaging functions.