65 results about "Dermal fibroblast" patented technology

The invention discloses Dong'e donkey-hide gelatin active peptide and application thereof. Relates to the technical field of biology. The invention provides an amino acid sequence and application of the Dong'e donkey-hide gelatin active peptide. The amino acid sequence of the CCA active peptide is YYTSASGDEMVSLK, and the CCA active peptide can significantly up-regulate the I-type collagen gene expression, increase the I-type and III-type collagen content and reduce senescence markers. The peptide fragment can be combined with TGFBR2, especially TGFBR1, gene and protein expression of the TGFBR2 can be up-regulated, downstream targets of a TGF-beta-SMAD pathway can be activated, and the MMPs / TIMPs ratio can be reduced. According to the present invention, the TGF beta-SMAD pathway is activated, the MMPs / TIMPs ratio is reduced, the collagen synthesis is promoted, and the excessive decomposition of the collagen is inhibited, such that the I type collagen content and the III type collagen content are increased, and the aging degree of the photoaging dermal fibroblast is reduced;

The invention discloses an anti-inflammatory and anti-aging composition containing a crocus sativus extract and application of the anti-inflammatory and anti-aging composition, and relates to the technical field of cosmetics, the anti-inflammatory and anti-aging composition comprises the crocus sativus extract, a kangaroo claw extract, a dendrobium devonianum stem extract and adenosine; the anti-inflammatory and anti-aging composition can promote human dermal fibroblasts to secrete III-type collagen, can promote human dermal fibroblasts to proliferate and improve the anti-oxidation and anti-inflammatory capacity of the human dermal fibroblasts, has certain anti-inflammatory and anti-aging effects, and can be widely applied to cosmetics with the anti-inflammatory and anti-aging effects.

The invention provides a beautifying composition and application thereof in whitening, anti-inflammatory and anti-aging products, and belongs to the technical field of cosmetics. The beautifying composition is prepared from collagen peptide, sodium hyaluronate and ergothioneine, wherein the mass concentration ratio of the collagen peptide to the sodium hyaluronate to the ergothioneine is (75 to 85) to (3.5 to 10) to (0.4 to 3.5); the beautifying composition shows remarkable advantages in the aspects of collagen regeneration and skin improvement, the collagen content can be remarkably increased, and multi-effect collagen regeneration promotion is achieved by inhibiting collagen degrading enzyme MMP-1 and up-regulating TGF-beta1; the content of AQP3 and HA can be increased, and water locking and moisturizing of the skin are enhanced; by activating an Nrf2 pathway, the SOD2 level is improved, IL-6 and IL-1alpha are inhibited, and the antioxidant, anti-inflammatory and whitening effects are achieved; the thickness of an epidermal layer and the density of dermal fibroblasts can be remarkably improved, wrinkle resistance and repairing are effectively achieved, and the skin structure is improved.

The present application provides gastrodia elata (Blume) alkaloids PFT201A, PFT201B and PFT203 and a preparation method thereof, and application thereof in the fields of cosmetics and medicine. Gastrodia elata Gastrodia elata (Blume) alkaloids PFT201A, PFT201B and PFT203 significantly promote the secretion of collagen type II in human dermal fibroblasts-adult (HDFa) at an extremely low concentration (0.1 μ M), and have no cytotoxicity at the concentration. These compounds can be used for preparing anti-aging drugs or skin care products.

This invention provides a method for obtaining ingredients from Dulong Paris polyphylla (… Paris dulongensis A method for preparing parisyunnanoside G (PFE55) in H. Li & Kurita and its application in cosmetics and pharmaceuticals. This research belongs to the field of cosmetics and pharmaceutical technology. PFE55 is used at extremely low concentrations (0.1... μ At a concentration of g / mL, it exhibits activity in promoting collagen secretion in adult dermal fibroblasts (HDFa) and is non-cytotoxic at this concentration, making it suitable for the preparation of anti-aging drugs or skincare products.

The invention provides a pedicel and inflorescence extract ZTM100 of Gastrodia elata Blume, a preparation method of the pedicel and inflorescence extract ZTM100 and application of the pedicel and inflorescence extract ZTM100 in the technical field of cosmetics and medicines. Belongs to the technical field of cosmetics and medicines. The ZTM100 (100 [mu] g / mL) provided by the invention can significantly improve the expression quantity of human dermal fibroblast elastin gene Elastin under UVA radiation, and does not show obvious cytotoxicity to human fibroblasts in a concentration range of 800 [mu] g / mL. The ZTM100 can be used for preparing medicines or cosmetics for resisting skin aging caused by UVA, reducing scar formation, repairing skin injury and repairing wounds.

The invention belongs to the technical field of biological medicine, and particularly discloses a small molecule compound SR4370 for promoting hair follicle growth and regeneration and application thereof. High-throughput screening finds that SR4370 can induce dermal fibroblasts to be reprogrammed into DP-like cells with characteristics of hair papilla cells and up-regulate ALP, WNT5A and other marker genes, and in-vivo and in-vitro experiments prove that SR4370 can activate resting hair follicles, promote in-vitro hair follicle growth and enhance the regeneration capacity of dermal stem cells. The invention provides a safe and efficient hair follicle regeneration strategy, and provides a new tool for alopecia treatment and tissue engineering.

The invention relates to the technical field of cosmetics, in particular to a compound active peptide with skin anti-aging, anti-inflammatory and soothing functions and application of the compound active peptide. The compound active peptide is prepared from acetyl hexapeptide-1, palmitoyl tripeptide-5, acetyl dipeptide-1 cetyl ester, copper peptide and decarboxylated carnosine hydrochloride. The compound peptide acts on dermal fibroblasts, so that expression of p16 and p21 genes is reduced, and cell cycle arrest is improved; the compound acts on epidermal keratinocytes and dermal fibroblasts and inhibits COX-2 gene expression; the compound peptide acts on epidermal keratinocytes and dermal fibroblasts, and the ROS level is reduced; the compound peptide acts on PC12 cells, inhibits secretion of acetylcholine, blocks transmission of neural signals, and plays a role in immediate wrinkle removal.

The invention provides an application of active compounds PTM39A and PTM40 in pedicels and inflorescences of Gastrodia elata Blume in the preparation of medicines and cosmetics. The active compounds PTM39A and PTM40 in the pedicels and the inflorescences of Gastrodia elata Blume are used for preparing medicines and cosmetics. Belongs to the technical field of cosmetics and medicines. According to the present invention, the compound PTM39A and the compound PTM40 can significantly promote the secretion of the type I collagen in the adult dermal fibroblasts (human dermis fibroblasts, HDFa) at the extremely low concentration (1 [mu] g / mL), and have no cytotoxicity at the concentration, such that the compound PTM39A and the compound PTM40 can significantly promote the secretion of the type I collagen in the human dermis fibroblasts (HDFa), and can significantly promote the secretion of the type I collagen in the human dermis fibroblasts (HDFa), the compound can be used for preparing anti-aging medicines or skin care products.

The present application belongs to the field of biomedical technology, and particularly relates to a cell-derived extracellular matrix and a preparation method and application of a cell culture plate coated with the cell-derived extracellular matrix. The present application cultures human astrocytes in a cell culture plate, and makes the human astrocytes secrete extracellular matrix. After the human astrocytes are added into a cell lysate for cell removal treatment, nucleic acid fragmentation treatment is performed to obtain the cell-derived extracellular matrix. The present application has relatively simple operation, short time period, and can realize batch production of extracellular matrix without external virus carrying and stable components. The obtained cell-derived extracellular matrix is resistant to storage, has good stability, is highly similar to a natural brain environment when coated on a cell culture plate, has good biocompatibility with cells, can be applied to tissue repair and regeneration as an ideal biomedical material, can regulate the progress and efficiency of human dermal fibroblast HDF transdifferentiation into neurons, and can obtain in-vitro survival neurons.

Disclosed herein are topical compositions comprising Centella asiatica extract and mandelic acid, wherein the compositions have a pH of less than 5, and methods of using the compositions to decrease cellular senescence in skin and treat dermatoporosis. Topical compositions comprising Centella asiatica extract and mandelic acid are effective to reduce the number of senescent cells in mammalian dermis, reduce the senescence-associated secretory phenotype (SASP), downregulate at least one SASP-associated gene, increase the expression of at least one cell cycle-related gene in dermal fibroblasts, downregulate at least one gene associated with skin inflammation, and increase fibroblast cell turnover.

The present invention belongs to the technical field of tissue damage repair, and specifically relates to a conductive hydrogel mediated by aminated graphene quantum dots, and its preparation method and application. The conductive hydrogel mediated by aminated graphene quantum dots provided by the present invention comprises a hydrogel obtained by photocuring acrylamide monomers, and aminated graphene quantum dots dispersed in the hydrogel; the mass of the aminated graphene quantum dots accounts for ≤0.3% of the mass of the acrylamide monomers. When the conductive hydrogel provided by the present invention is combined with exogenous electrical stimulation, an external current is applied to the wound to simulate the endogenous current at the wound, guiding dermal fibroblasts (DFs) to migrate and proliferate toward the wound along the electrical gradient, stimulating tissue growth, and thus accelerating wound healing.

Provided herein are compositions for skin lightening and for the prevention and / or treatment of skin pigmentation disorders. Also provided herein are methods and uses for preventing and / or treating skin pigmentation disorders, for inhibiting melanin production in epidermal melanocytes and / or promoting melanin degradation in dermal fibroblasts, and non-therapeutic methods for lightening complexion.

The invention discloses a composite PCL (Polycaprolactone) microsphere as well as a preparation method and application thereof. The ratio of the shell thickness to the core radius of the composite PCL microsphere provided by the invention is 1: 1-1: 3, and the particle size of the composite PCL microsphere is 20-65 [mu] m. The PCL polycaprolactone microspheres provided by the invention can make dermal fibroblasts continuously proliferate in low inflammatory response, so that the fibroblasts can orderly grow around the composite microspheres, the overall inflammatory level is reduced, collagen and extracellular matrix are continuously generated, and the regeneration effect is achieved.

This invention proposes a nucleic acid aptamer that specifically binds to human senescent dermal fibroblasts (HDFs), along with its screening, identification, and application. Specific aptamers exhibiting high targeting specificity to human senescent HDFs were screened using Cell-SELEX technology. The three selected candidate aptamers showed binding rates of 57.5%, 86.2%, and 50.7% to senescent HDF cells within the same timeframe, respectively, demonstrating high specificity and targeted binding ability. Furthermore, the aptamer possesses potential for detecting, imaging, and regulating the function of senescent cells, successfully providing a new direction for targeted anti-aging interventions and therapeutic applications.

The invention provides a Paris polyphylla (Takht.) H. Li] extract product PCT100, and an application of the Paris polyphylla (Takht.) H. Li] extract product PCT100 in the technical fields of cosmetics and medicines. Belongs to the technical field of cosmetics and medicines. The PCT100 (0.4 [mu] g / mL) provided by the invention can significantly improve the expression quantity of the human dermal fibroblast elastin gene Elastin under UVA radiation, and does not show obvious cytotoxicity to human fibroblasts in a concentration range of 3.2 [mu] g / mL. The PCT100 can be used for preparing medicines or cosmetics for resisting skin aging caused by UVA, repairing wounds, reducing scar formation, repairing skin injury and promoting elastin gene expression and synthesis.

The present invention discloses the application of hydrolyzed aloe flower protein in skin repair products. The amino acid sequence of the hydrolyzed aloe flower protein is at least one of Leu-Pro-Phe, Leu-Gly, and Ala-Phe. The hydrolyzed aloe flower protein of the present invention improves dermal homeostasis, especially the functional homeostasis of dermal fibroblasts, by enhancing the proliferation and migration abilities of dermal fibroblasts, promoting the synthesis of extracellular matrix components of dermal fibroblasts, and releasing fibroblast growth factors, thereby achieving the purpose of skin repair.

The application provides application of a carrot-derived extracellular vesicle-like particle in preparation of a skin wound repair product, and belongs to the technical field of biological medicine. The extracellular vesicle-like particle has good water dispersibility and low-temperature storage stability; can be taken by dermal fibroblasts, vascular endothelial cells and the like, can reduce cell apoptosis, improve cell activity, promote proliferation, migration and blood vessel formation related functions under high-sugar high-fat and the like damage conditions, thereby synergistically improving the functions of dermal fibroblasts and vascular endothelial cells, and has certain antibacterial capacity, thereby being beneficial to skin wound healing, and providing a scheme and a clinical conversion idea for wound treatment.

Identification of dermal fibroblast subpopulations. [Solution] The object of the present invention is a method for identifying dermal fibroblasts in vitro as papillary fibroblasts, reticular fibroblasts or dermal-subcutaneous junction fibroblasts (FJDH), the method comprising a step of measuring the levels of expression products of UCP2 and FGF9, and optionally at least one gene selected from the group consisting of the COL11A1 gene and the ACAN gene, and the level of expression product of the KLF9 gene.

The invention discloses an anti-wrinkle composition containing a camellia japonica extract and capable of promoting cell energy and an application of the anti-wrinkle composition. The anti-wrinkle composition is prepared from the following components in percentage by mass: 5 to 20 percent of camellia japonica extract, 7.4 to 7.6 percent of flaxseed oil, 0.14 to 0.16 percent of sorbitan trioleate, 0.005 to 0.007 percent of celery seed extract, 0.004 to 0.006 percent of crithmum maritimum extract, 0.003 to 0.004 percent of flaxseed extract and the balance of caprylic / capric triglyceride which is supplemented to 100 percent. The invention further discloses application of the anti-wrinkle composition in promoting synthesis of NAD < + > and ATP in dermal fibroblasts and / or epidermal keratinocytes and / or SIRT3 gene expression and application of the anti-wrinkle composition in preparing cosmetics or skin care products with anti-wrinkle and tightening effects. The composition disclosed by the invention is an oil-soluble composition, is easier to absorb, and can improve the mitochondrial activity through synergistic interaction.

Disclosed herein are topical compositions comprising an extract of centella asiatica and mandelic acid, where the pH of the composition is less than 5, and methods of using the compositions to reduce cellular senescence in the skin and to treat cutis laxa. A topical composition comprising a centella asiatica extract and mandelic acid is effective in reducing the number of senescent cells in mammalian dermis, reducing senescence-related secretory phenotypes (SASP), down-regulating at least one SASP-related gene, increasing expression of at least one cell cycle-related gene in dermal fibroblasts, and reducing the number of senescent cells in mammalian dermis. At least one gene associated with skin inflammation is down-regulated and the fibroblast turnover rate is increased.

Disclosed herein are topical compositions comprising Centella asiatica extract and mandelic acid, wherein the compositions have a pH of less than 5, and methods of using the compositions to decrease cellular senescence in skin and treat dermatoporosis. Topical compositions comprising Centella asiatica extract and mandelic acid are effective to reduce the number of senescent cells in mammalian dermis, reduce the senescence-associated secretory phenotype (SASP), downregulate at least one SASP-associated gene, increase the expression of at least one cell cycle-related gene in dermal fibroblasts, downregulate at least one gene associated with skin inflammation, and increase fibroblast cell turnover.

The invention discloses an extraction and preparation method of green tea exosome-like nano-vesicles, and particularly relates to the field of application of natural active substances, the green tea exosome-like nano-vesicles are obtained in the form of a green tea composition, and compared with exosome-like nano-vesicles of green tea from a single source, the green tea exosome-like nano-vesicles are more in quantity and rich in tea polyphenol; the green tea composition exosome-like nanocapsule prepared by the invention has the beneficial effects in the field of cosmetics; as an anti-oxidation and anti-aging skin care product, the skin care product has the following beneficial effects: 1) excessive endogenous ROS and RNS in the body are eliminated; 2) the contents of collagen and elastic fibers are increased; (3) the production of collagen degrading enzyme MMP-3 in the skin is inhibited; 4) the significant inhibitory activity of alpha-glucosidase; 5) reducing TNF-induced MMP-1 expression, and inhibiting ERK signal transduction; and 6) inhibiting phosphorylation of MEK and Src in human dermal fibroblasts.

The present application relates to the use of a substance that regulates BCL11A gene and / or its encoded product in the preparation of a drug for reversing skin photoaging, wherein the skin photoaging includes skin cell aging caused by sunlight, light, etc., and the skin cells include keratinocytes, dermal fibroblasts, melanocytes. The regulation is to up-regulate the expression of BCL11A gene and / or promote the production of its encoded product. Typical clinical manifestations of the skin photoaging include wrinkles, dry skin, loose skin, dilated capillaries, uneven skin pigmentation, and skin itching. The substance that regulates BCL11A gene and / or its encoded product includes a substance that regulates BCL11A gene DNA molecules and a substance that regulates the expression of BCL11A translation product protein molecules. The reversal of skin photoaging is manifested in reducing the proportion of cells positive for aging-related beta-galactosidase staining in skin cells and reducing the expression of aging-related protein p16 and DNA damage protein gammaH2AX.

Application of mulberry bark effective part in whitening and anti-UVA damage and preparation method thereof, the effective part is that mulberry bark is extracted with ethanol, the obtained extract is concentrated under reduced pressure, then dissolved with ethanol, then adsorbed with macroporous resin, eluted with different concentrations of ethanol, different concentrations of ethanol eluate is collected respectively, then concentrated under reduced pressure to obtain the effective part. α The protective effect of the effective part on UVA damaged human dermal fibroblasts (HDF- α ) is equivalent to that of the positive control vitamin C (100 μM). The effective part (0.3% emulsion) shows significant whitening effect in ultraviolet irradiation test population, which is equivalent to that of the positive control vitamin C (7% emulsion). The effective part of the application has good application prospect in developing whitening, anti-photo damage or double effect cosmetics.

The purpose of the present invention is to identify a dermal fibroblast aging subpopulation. The present invention has reclassified dermal fibroblasts using a single-cell analysis technique, and upon doing so, has discovered a novel subpopulation that has not previously been classified, on the basis of differences in the gene expression of dermal fibroblasts which were isolated from tissue. This subpopulation is contained in a large amount in the skin of an elderly subject, and is also contained in the skin of a young subject. The subpopulation is identified as an aging subpopulation associated with the aging of the skin.

The application provides a novel human type II collagen COLII-14K-H and a coding gene thereof, the coding gene is shown as SEQ ID NO. 1, and the amino acid sequence is shown as SEQ ID NO. 2. The application also provides a preparation method of the human type II collagen COLII-14K-H and application thereof in preparing cartilage repair drugs, skin repair drugs and skin care products. The human type II collagen can significantly improve the collagen gene expression level, realize the improvement of the content of collagen in human dermal fibroblasts, and delay skin aging. Meanwhile, the protein fragment can exhibit a significant skin repair effect at a low concentration, provides a new treatment option for skin repair, and can be widely applied in the fields of skin repair and care.

The invention relates to application of Antarctic red in a skin conditioning agent for synergistically controlling oil and resisting wrinkles, and belongs to the technical field of biology, the Antarctic red has no toxicity to cells when the concentration is not higher than 250 g / mL, gene expression of I-type 5 alpha-reductase in sebaceous gland cells is inhibited in a targeted mode, and excessive synthesis of grease is reduced from the source; the gene expression level of I type and III type collagen in dermal fibroblasts is synchronously improved, so that the internal support structure of the skin is reconstructed, and the anti-wrinkle effect is realized. According to the Antarctic red disclosed by the invention, through the synergistic effect of'descending and ascending ', the Antarctic red is proved to be capable of simultaneously and accurately intervening an'oil-wrinkle' associated pathway, and the limitation of a single functional component is broken through.

The application discloses an antibacterial polypeptide and a preparation method and application thereof, and the antibacterial polypeptide has an amino acid sequence as shown in any one of SEQ ID NO:1-2. The antibacterial polypeptide prepared by the scheme of the application has good inhibiting and sterilizing effects on pseudomonas aeruginosa and the fungus candida albicans. Meanwhile, the antibacterial polypeptide L18N6P obtained through design has no toxic side effect on cells, can even promote the proliferation of human dermal fibroblasts, has good biocompatibility, has the potential to become a drug for treating diseases caused by pseudomonas aeruginosa or candida albicans infection or to prepare antibacterial medical device materials, is not prone to drug resistance, and has high application value.

Disclosed herein are topical compositions comprising Centella asiatica (Centella asiatica) extract and mandelic acid, wherein the composition has a pH of less than 5, and methods of using the compositions to reduce cellular senescence in the skin and treat dermatoporosis. The topical composition comprising Centella asiatica extract and mandelic acid is effective in reducing the number of senescent cells in mammalian dermis, reducing the senescence-associated secretory phenotype (SASP), downregulating at least one SASP-related gene, increasing the expression of at least one cell cycle-related gene in dermal fibroblasts, downregulating at least one gene associated with skin inflammation, and increasing fibroblast turnover.