114 results about "Dermal fibroblast" patented technology

The invention belongs to the field of cosmetic detection, and discloses a cosmetic and cosmetic raw material sunscreen function evaluation method and application. According to the evaluation method, a sunscreen effect test is carried out on a test sample by using a non-contact administration device, wherein the test sample is a cosmetic finished product or a diluted cosmetic raw material; the test comprises evaluation of in-vitro sunscreen effect by skin cells, evaluation of in-vivo sunscreen effect by a zebra fish embryo model and result evaluation. According to the method, the sunburn-resistant effect is evaluated through expression of beta-galactosidase in human dermal fibroblasts and / or secretion of collagen, the sunburn-resistant effect is evaluated through the survival rate of human keratinocytes, the DNA damage condition or inflammatory factor secretion and the lethal / teratogenic condition of zebra fish embryos, and multi-layer and all-around sunburn-resistant effect evaluation is achieved. The method provided by the invention is suitable for evaluating the sunscreen function of various sunscreen products, has accuracy and practicability, and provides an important technical basis for research and development, quality control and performance optimization of sunscreen cosmetics.

The invention provides a novel human-derived II-type collagen COLII-14K-H and a coding gene thereof, the coding gene is shown as SEQ ID NO.1, and the amino acid sequence is shown as SEQ ID NO.2. The invention further provides a preparation method of the novel human-derived II-type collagen COLII-14K-H. The invention also provides a preparation method of the human-derived II-type collagen COLII-14K-H and application of the human-derived II-type collagen COLII-14K-H in preparation of cartilage repair drugs, skin repair drugs and skin care products. The inventor-derived II-type collagen can obviously improve the gene expression level of the collagen, so that the content of the collagen in human dermal fibroblasts is improved, and the skin aging is delayed; meanwhile, the protein fragment can show a remarkable skin repairing effect at low concentration, provides a new treatment choice for skin repairing, and can be widely applied to the fields of skin repairing and nursing.

The present invention belongs to the field of cosmetics detection, and discloses a method for evaluating the sun protection function of cosmetics and cosmetic raw materials and its application. This evaluation method uses a non-contact drug delivery device to test the sun protection effect of the test samples, and the test samples are finished cosmetics or diluted cosmetic raw materials; the tests include evaluating the in vitro sun protection efficacy by skin cells, evaluating the in vivo sun protection efficacy by the zebrafish embryo model, and result evaluation. This method evaluates the anti-aging effect of sun protection through the expression of β-galactosidase and / or the secretion of collagen in human dermal fibroblasts, and evaluates the sunburn prevention effect through the survival rate of human keratinocytes, DNA damage, or the secretion of inflammatory factors and the lethal / teratogenic conditions of zebrafish embryos, realizing multi-level and all-round evaluation of the sun protection efficacy. The method provided by the present invention is applicable to the evaluation of the sun protection function of various sun protection products, has accuracy and practicability, and provides an important technical basis for the research and development, quality control, and performance optimization of sun protection cosmetics.

The invention relates to the technical field of gene engineering, in particular to application of a detection reagent for a hair follicle development related gene of a down producing goat. According to the present invention, the Cashmere goat hair follicle development related gene is any one or more of lncRNA MSTRG.20890.1, chi-miR-24-3p and ADAMTS3, and the lncRNA MSTRG.20890.1 and the ADAMTS3 are competitively combined with the chi-miR-24-3p, such that the significant differential expression of the lncRNA MSTRG.20890.1 in the Cashmere goat secondary hair follicle morphogenesis process is found, and the results prove that the lncRNA MSTRG.20890.1 can regulate the proliferation and the migration of dermal fibroblasts through the competitive combination of the lncRNA MSTRG.20890.1 and the ADAMTS3, such that the growth of the Cashmere goat secondary hair follicle morphogenesis can be inhibited therefore, the formation of a dermal papilla structure is inhibited.

The invention relates to application of a white mulberry root-bark effective part in whitening and UVA damage resistance and a preparation method of the white mulberry root-bark effective part. The white mulberry root-bark effective part is obtained by extracting white mulberry root-bark with ethanol, concentrating the obtained extracting solution under reduced pressure, dissolving the concentrated extracting solution with ethanol, adsorbing with macroporous resin, eluting with ethanol with different concentrations, respectively collecting ethanol eluates with different concentrations, and concentrating under reduced pressure. The protection effect of the effective part on UVA damaged human dermal fibroblasts (HDF-alpha) is equivalent to that of positive control vitamin C (100 mu M). The effective part (0.3% emulsion) shows a remarkable whitening effect in ultraviolet radiation tested people, and the effect of the effective part is equivalent to that of positive control vitamin C (7% emulsion). The effective part disclosed by the invention has a better application prospect in developing cosmetics with whitening, light damage resistance or dual effects.

The invention discloses Dong'e donkey-hide gelatin active peptide and application thereof. Relates to the technical field of biology. The invention provides an amino acid sequence and application of the Dong'e donkey-hide gelatin active peptide. The amino acid sequence of the CCA active peptide is YYTSASGDEMVSLK, and the CCA active peptide can significantly up-regulate the I-type collagen gene expression, increase the I-type and III-type collagen content and reduce senescence markers. The peptide fragment can be combined with TGFBR2, especially TGFBR1, gene and protein expression of the TGFBR2 can be up-regulated, downstream targets of a TGF-beta-SMAD pathway can be activated, and the MMPs / TIMPs ratio can be reduced. According to the present invention, the TGF beta-SMAD pathway is activated, the MMPs / TIMPs ratio is reduced, the collagen synthesis is promoted, and the excessive decomposition of the collagen is inhibited, such that the I type collagen content and the III type collagen content are increased, and the aging degree of the photoaging dermal fibroblast is reduced;

The invention provides a Dendrobium officinale Kimura amp (MIG) leaf extract, a preparation method of the Dendrobium officinale Kimura amp (MIG) leaf extract, and application of the Dendrobium officinale Kimura amp (MIG) leaf extract in the fields of cosmetics and medicines. Belongs to the technical field of cosmetics and medicines. The dendrobium officinale leaf extract WYH169, the dendrobium officinale leaf extract WYH2202, the dendrobium officinale leaf extract WYH205 and the dendrobium officinale leaf extract WYH328 have the effect of promoting collagen secretion in human dermis fibroblasts (human dermis fibroblasts, HDFa), and can be used for preparing anti-aging drugs or skin care products, so that the dendrobium officinale leaf extract WYH169, the dendrobium officinale leaf extract WYH2202, the dendrobium officinale leaf extract WYH205 and the dendrobium officinale leaf extract WYH328 can be used for preparing anti-aging drugs or skin care products.

The invention discloses an anti-inflammatory and anti-aging composition containing a crocus sativus extract and application of the anti-inflammatory and anti-aging composition, and relates to the technical field of cosmetics, the anti-inflammatory and anti-aging composition comprises the crocus sativus extract, a kangaroo claw extract, a dendrobium devonianum stem extract and adenosine; the anti-inflammatory and anti-aging composition can promote human dermal fibroblasts to secrete III-type collagen, can promote human dermal fibroblasts to proliferate and improve the anti-oxidation and anti-inflammatory capacity of the human dermal fibroblasts, has certain anti-inflammatory and anti-aging effects, and can be widely applied to cosmetics with the anti-inflammatory and anti-aging effects.

The invention belongs to the technical field of biological protein, and particularly relates to a cytological detection method of recombinant three-type humanized collagen, which comprises the following steps: S1, culturing cells: inoculating human dermal fibroblasts into a culture medium, and culturing to a logarithmic phase for later use; s2, co-culture: co-culturing the standard recombinant three-type humanized collagen with different concentrations and the human dermal fibroblasts in the logarithmic production period; s3, constructing a standard curve; s4, quantifying and evaluating. The concentration of the target protein can be calculated according to the standard curve, the method is simple, expensive detection instruments are not needed, and the method is more practical and convenient. Meanwhile, dozens of samples can be detected in batches at the same time, and the detection efficiency is greatly improved. When the concentration of the target protein is quantitatively detected, the biological activity of the target protein can be evaluated through the cell adhesion rate, and compared with an existing detection method, more biological information of the target protein can be provided.

The invention relates to the technical field of biology, in particular to an engineered vesicle carrying mRNA targeting and application of the engineered vesicle to improvement of fibroblast functionality. The iRGD polypeptide carried by the engineered vesicle is used as a targeting polypeptide of fibroblasts, specifically targets human dermal fibroblasts and can be combined with rich integrin alpha v and NRP-1 on cell membranes of the fibroblasts, meanwhile, the iRGD polypeptide and exosome surface mask protein emp2b construct a fusion protein, MS2 capsid protein is fused in exosome, and the human dermal fibroblasts can be specifically targeted to the human dermal fibroblasts. According to the present invention, with the combination of the mRNA containing the MS2 stem loop, the more target mRNA can be obtained, such that the more target mRNA can enter the senescent fibroblast in the targeting manner so as to improve the activity of the fibroblast and delay the senescence of the human skin;

The invention provides a beautifying composition and application thereof in whitening, anti-inflammatory and anti-aging products, and belongs to the technical field of cosmetics. The beautifying composition is prepared from collagen peptide, sodium hyaluronate and ergothioneine, wherein the mass concentration ratio of the collagen peptide to the sodium hyaluronate to the ergothioneine is (75 to 85) to (3.5 to 10) to (0.4 to 3.5); the beautifying composition shows remarkable advantages in the aspects of collagen regeneration and skin improvement, the collagen content can be remarkably increased, and multi-effect collagen regeneration promotion is achieved by inhibiting collagen degrading enzyme MMP-1 and up-regulating TGF-beta1; the content of AQP3 and HA can be increased, and water locking and moisturizing of the skin are enhanced; by activating an Nrf2 pathway, the SOD2 level is improved, IL-6 and IL-1alpha are inhibited, and the antioxidant, anti-inflammatory and whitening effects are achieved; the thickness of an epidermal layer and the density of dermal fibroblasts can be remarkably improved, wrinkle resistance and repairing are effectively achieved, and the skin structure is improved.

The invention discloses a circadian rhythm-based cosmetic raw material in-vitro repair efficacy evaluation method, and belongs to the field of cosmetic evaluation, and the method comprises the following steps: S1, HDFA human dermal fibroblast culture, and RAW264.7 mouse macrophage culture; s2, determining the detection concentration of the cosmetic raw materials through cell viability detection; s3, inducing HDFA human dermal fibroblasts with blue light, and detecting the expression quantity of the clock gene BMAL1 and / or CLOCK; s4, after cell scratches of the HDFA human dermal fibroblasts are made, the HDFA human dermal fibroblasts are induced by blue light, and the healing area of the cell scratches is detected; s5, inducing the RAW264.7 mouse macrophage model by virtue of an induction drug lipopolysaccharide, and detecting the expression quantity of a clock gene Dbp and / or Rev-erbalpha; s6, processing and analyzing detection results of the steps S3 to S5, and scoring the detection results; and summarizing all detection index scores. Different cell model detection results are combined, the repairing function of the cosmetic raw materials is subjected to multi-dimensional evaluation, the evaluation accuracy is improved, and the evaluation process is rapid and efficient.

The invention provides a pedicel and inflorescence extract of Gastrodia elata Blume, a preparation method of the pedicel and inflorescence extract of Gastrodia elata Blume, and application of the pedicel and inflorescence extract of Gastrodia elata Blume in the fields of cosmetics and medicines. Belongs to the technical field of cosmetics and medicines. The gastrodia elata pedicel and inflorescence extracts ZTM-2, ZTM-4, ZTM-5, ZTM-6, ZTM-7 and ZTM-8 have the effect of promoting collagen secretion, and the ZTM-5 and the ZTM-6 have the activity of promoting collagen secretion in human dermis fibroblasts (human dermis fibroblasts, HDFa) at the extremely low concentration (1 mu g / mL), and have the activity of promoting collagen secretion in human dermis fibroblasts (human dermis fibroblasts, human dermis fibroblasts) at the extremely low concentration (1 mu g / mL) at the extremely low concentration (1 mu g / mL) at the extremely low concentration (1 mu g / mL) at the extremely low concentration (1 mu g / mL) at the extremely low concentration (1 mu g / mL). Under the concentration, the compound has no cytotoxicity and can be used for preparing anti-aging drugs or skin care products.

Nature-inspired smart materials offer numerous advantages over environment-friendliness and efficiency. Emulating the excellent adhesive properties of mussels foot proteins, where the Lysine is in close proximity with the 3, 4-dihydroxy-L-phenylalanine (DOPA), embodiments report the synthesis of a novel photo-curable peptide-based adhesive consisting exclusively of these two amino acids. The adhesive is a highly concentrated aqueous solution of a monomer, a crosslinker and a photoinitiator. No toxicity of the adhesive was observed when the cytocompatibility on human dermal fibroblast cells was assessed. Lap-shear adhesion measurements on plastic and glass surfaces and comparison with different types of commercial adhesives showed that the adhesive strength of the disclosed glue is comparable when applied in the air and superior when used underwater. Given the convenience of the facile synthesis, biocompatibility, ease of application underwater and high adhesive strength, embodiments expect that the adhesive may find application, but not limited, to the biomedical field.

The present application provides gastrodia elata (Blume) alkaloids PFT201A, PFT201B and PFT203 and a preparation method thereof, and application thereof in the fields of cosmetics and medicine. Gastrodia elata Gastrodia elata (Blume) alkaloids PFT201A, PFT201B and PFT203 significantly promote the secretion of collagen type II in human dermal fibroblasts-adult (HDFa) at an extremely low concentration (0.1 μ M), and have no cytotoxicity at the concentration. These compounds can be used for preparing anti-aging drugs or skin care products.

This invention provides a method for obtaining ingredients from Dulong Paris polyphylla (… Paris dulongensis A method for preparing parisyunnanoside G (PFE55) in H. Li & Kurita and its application in cosmetics and pharmaceuticals. This research belongs to the field of cosmetics and pharmaceutical technology. PFE55 is used at extremely low concentrations (0.1... μ At a concentration of g / mL, it exhibits activity in promoting collagen secretion in adult dermal fibroblasts (HDFa) and is non-cytotoxic at this concentration, making it suitable for the preparation of anti-aging drugs or skincare products.

The invention provides a pedicel and inflorescence extract ZTM100 of Gastrodia elata Blume, a preparation method of the pedicel and inflorescence extract ZTM100 and application of the pedicel and inflorescence extract ZTM100 in the technical field of cosmetics and medicines. Belongs to the technical field of cosmetics and medicines. The ZTM100 (100 [mu] g / mL) provided by the invention can significantly improve the expression quantity of human dermal fibroblast elastin gene Elastin under UVA radiation, and does not show obvious cytotoxicity to human fibroblasts in a concentration range of 800 [mu] g / mL. The ZTM100 can be used for preparing medicines or cosmetics for resisting skin aging caused by UVA, reducing scar formation, repairing skin injury and repairing wounds.

The invention belongs to the technical field of biological medicine, and particularly discloses a small molecule compound SR4370 for promoting hair follicle growth and regeneration and application thereof. High-throughput screening finds that SR4370 can induce dermal fibroblasts to be reprogrammed into DP-like cells with characteristics of hair papilla cells and up-regulate ALP, WNT5A and other marker genes, and in-vivo and in-vitro experiments prove that SR4370 can activate resting hair follicles, promote in-vitro hair follicle growth and enhance the regeneration capacity of dermal stem cells. The invention provides a safe and efficient hair follicle regeneration strategy, and provides a new tool for alopecia treatment and tissue engineering.

The invention relates to the technical field of cosmetics, in particular to a compound active peptide with skin anti-aging, anti-inflammatory and soothing functions and application of the compound active peptide. The compound active peptide is prepared from acetyl hexapeptide-1, palmitoyl tripeptide-5, acetyl dipeptide-1 cetyl ester, copper peptide and decarboxylated carnosine hydrochloride. The compound peptide acts on dermal fibroblasts, so that expression of p16 and p21 genes is reduced, and cell cycle arrest is improved; the compound acts on epidermal keratinocytes and dermal fibroblasts and inhibits COX-2 gene expression; the compound peptide acts on epidermal keratinocytes and dermal fibroblasts, and the ROS level is reduced; the compound peptide acts on PC12 cells, inhibits secretion of acetylcholine, blocks transmission of neural signals, and plays a role in immediate wrinkle removal.

The invention discloses repair hydrogel as well as a preparation method and application thereof. The raw materials of the repairing hydrogel comprise 3D-ECM, 3D-EVs and sodium hyaluronate, the 3D-ECM is an epimatrix extracted by 3D culture of human dermal fibroblasts in a hydrogel scaffold, and the 3D-EVs is an epivesicle extracted by 3D culture of human stem cells in the hydrogel scaffold. The repairing hydrogel provides a good example for the application of the 3D-ECM and the 3D-EVs; the repair hydrogel is rich in more than 80 kinds of protein and polysaccharide substances beneficial to regeneration and repair of the articular cartilage, and has a good effect of promoting repair of the articular cartilage injury.

The invention provides an application of active compounds PTM39A and PTM40 in pedicels and inflorescences of Gastrodia elata Blume in the preparation of medicines and cosmetics. The active compounds PTM39A and PTM40 in the pedicels and the inflorescences of Gastrodia elata Blume are used for preparing medicines and cosmetics. Belongs to the technical field of cosmetics and medicines. According to the present invention, the compound PTM39A and the compound PTM40 can significantly promote the secretion of the type I collagen in the adult dermal fibroblasts (human dermis fibroblasts, HDFa) at the extremely low concentration (1 [mu] g / mL), and have no cytotoxicity at the concentration, such that the compound PTM39A and the compound PTM40 can significantly promote the secretion of the type I collagen in the human dermis fibroblasts (HDFa), and can significantly promote the secretion of the type I collagen in the human dermis fibroblasts (HDFa), the compound can be used for preparing anti-aging medicines or skin care products.

The invention provides a medicine for treating skin photoaging. The medicine for treating skin photoaging comprises extracellular vesicles derived from adipose tissue. The medicine for treating skin photoaging provided by the invention can recover the activity of human dermal fibroblasts damaged by UVB, including improvement of cell proliferation and migration ability, stabilization of cell cycle, reduction of generation of reactive oxygen species (ROS) and reduction of expression of aging markers.

The present application belongs to the field of biomedical technology, and particularly relates to a cell-derived extracellular matrix and a preparation method and application of a cell culture plate coated with the cell-derived extracellular matrix. The present application cultures human astrocytes in a cell culture plate, and makes the human astrocytes secrete extracellular matrix. After the human astrocytes are added into a cell lysate for cell removal treatment, nucleic acid fragmentation treatment is performed to obtain the cell-derived extracellular matrix. The present application has relatively simple operation, short time period, and can realize batch production of extracellular matrix without external virus carrying and stable components. The obtained cell-derived extracellular matrix is resistant to storage, has good stability, is highly similar to a natural brain environment when coated on a cell culture plate, has good biocompatibility with cells, can be applied to tissue repair and regeneration as an ideal biomedical material, can regulate the progress and efficiency of human dermal fibroblast HDF transdifferentiation into neurons, and can obtain in-vitro survival neurons.

Disclosed herein are topical compositions comprising Centella asiatica extract and mandelic acid, wherein the compositions have a pH of less than 5, and methods of using the compositions to decrease cellular senescence in skin and treat dermatoporosis. Topical compositions comprising Centella asiatica extract and mandelic acid are effective to reduce the number of senescent cells in mammalian dermis, reduce the senescence-associated secretory phenotype (SASP), downregulate at least one SASP-associated gene, increase the expression of at least one cell cycle-related gene in dermal fibroblasts, downregulate at least one gene associated with skin inflammation, and increase fibroblast cell turnover.

The present invention belongs to the technical field of tissue damage repair, and specifically relates to a conductive hydrogel mediated by aminated graphene quantum dots, and its preparation method and application. The conductive hydrogel mediated by aminated graphene quantum dots provided by the present invention comprises a hydrogel obtained by photocuring acrylamide monomers, and aminated graphene quantum dots dispersed in the hydrogel; the mass of the aminated graphene quantum dots accounts for ≤0.3% of the mass of the acrylamide monomers. When the conductive hydrogel provided by the present invention is combined with exogenous electrical stimulation, an external current is applied to the wound to simulate the endogenous current at the wound, guiding dermal fibroblasts (DFs) to migrate and proliferate toward the wound along the electrical gradient, stimulating tissue growth, and thus accelerating wound healing.

Provided herein are compositions for skin lightening and for the prevention and / or treatment of skin pigmentation disorders. Also provided herein are methods and uses for preventing and / or treating skin pigmentation disorders, for inhibiting melanin production in epidermal melanocytes and / or promoting melanin degradation in dermal fibroblasts, and non-therapeutic methods for lightening complexion.

The invention discloses a composite PCL (Polycaprolactone) microsphere as well as a preparation method and application thereof. The ratio of the shell thickness to the core radius of the composite PCL microsphere provided by the invention is 1: 1-1: 3, and the particle size of the composite PCL microsphere is 20-65 [mu] m. The PCL polycaprolactone microspheres provided by the invention can make dermal fibroblasts continuously proliferate in low inflammatory response, so that the fibroblasts can orderly grow around the composite microspheres, the overall inflammatory level is reduced, collagen and extracellular matrix are continuously generated, and the regeneration effect is achieved.

To provide pharmaceutical compositions that enhance hyaluronic acid synthesis aiming at effective utilization of and value addition to pomegranate seeds to be disposed.SOLUTION: The invention provides a pharmaceutical composition for enhancing hyaluronic acid synthesis that comprises a pomegranate seed extract. In a preferred embodiment, the pharmaceutical composition for enhancing hyaluronic acid synthesis enhances the synthesis of hyaluronic acid in dermal fibroblasts. In a preferred embodiment, the pharmaceutical composition for enhancing hyaluronic acid synthesis increases the expression of a hyaluronic acid synthesizing enzyme, where the hyaluronic acid synthesizing enzyme is HAS-1 or HAS-2.SELECTED DRAWING: Figure 1

To provide pharmaceutical compositions for suppressing elastin fiber decomposition to achieve effective utilization of and value addition to disposed pomegranate seeds.SOLUTION: The pharmaceutical compositions for suppressing elastin fiber decomposition of the invention comprise a pomegranate seed extract. In a preferred embodiment of the pharmaceutical compositions for suppressing elastin fiber decomposition, the pharmaceutical composition suppresses elastin fiber decomposition in the dermal fibroblasts. In a preferred embodiment of the pharmaceutical compositions for suppressing elastin fiber decomposition, the pharmaceutical composition inhibits activities of elastin-degrading enzymes.SELECTED DRAWING: Figure 1

This invention proposes a nucleic acid aptamer that specifically binds to human senescent dermal fibroblasts (HDFs), along with its screening, identification, and application. Specific aptamers exhibiting high targeting specificity to human senescent HDFs were screened using Cell-SELEX technology. The three selected candidate aptamers showed binding rates of 57.5%, 86.2%, and 50.7% to senescent HDF cells within the same timeframe, respectively, demonstrating high specificity and targeted binding ability. Furthermore, the aptamer possesses potential for detecting, imaging, and regulating the function of senescent cells, successfully providing a new direction for targeted anti-aging interventions and therapeutic applications.