66 results about "Epidermal keratinocyte" patented technology

Epidermal keratinocytes not incorporating magnetic particles therein and epidermal keratinocytes incorporating magnetic particles therein were seeded in a 24-well ultra-low-attachment plate, and cultured for one day after a neodymium magnet was placed outside the bottom of the well. As a result, control epidermal keratinocytes not incorporating magnetic particles therein did not adhere to the bottom of the well and did not form an undifferentiated cell sheet. On the contrary, epidermal keratinocytes incorporating magnetic particles therein, while being attracted to the bottom of the well by magnetic force, formed a multilayered cell sheet. This cell sheet could be easily recovered by removing the neodymium magnet placed outside the bottom of the well, then bringing a magnet having a hydrophilic film attached thereto close to the cell sheet from upside and to attract it via this hydrophilic film, and lifting it.

The present inventors revealed for the first time in the world that 1) a large amount of bone marrow-derived cells are mobilized in transplanted skin; 2) the mobilized bone marrow-derived cells are differentiated into any of dermal fibroblasts, adipocytes, muscle cells, vascular endothelial cells and epidermal keratinocytes in a transplanted skin graft, and in the mobilized bone marrow-derived cells, bone marrow-derived mesenchymal stem cells are contained; 3) what mobilize the bone marrow-derived mesenchymal stem cells to the graft from the peripheral blood are HMGB1, HMGB2 and HMGB3 released from necrotic tissue of the transplanted skin; 4) purified HMGB1, HMGB2 and HMGB3 promote migration of mesenchymal stem cells isolated from the bone marrow and cultured; 5) active substances including HMGB1 that allow bone marrow mesenchymal stem cells to migrate can be simply purified from an extract of a large number of organs including skin, brain and heart; 6) active substances that allow bone marrow mesenchymal stem cells to migrate can be simply extracted from cultured cells; and 7) a heparin column-purified fraction of skin extract mobilizes a large amount of bone marrow-derived cells during brain damage.

The present inventors revealed the following for the first time in the world:1) a large amount of bone marrow-derived cells are mobilized to grafted skin;2) the mobilized bone marrow-derived cells are differentiated into any of dermal fibroblasts, adipocytes, muscle cells, vascular endothelial cells, and epidermal keratinocytes in the grafted skin, and the mobilized bone marrow derived cells include bone marrow-derived mesenchymal stem cells;3) the factors which mobilize bone marrow-derived mesenchymal stem cells from peripheral blood to the grafted skin are HMGB1, HMGB2, and HMGB3 released from the necrosed tissue of recipient skin;4) purified HMGB1, HMGB2, and HMGB3 promote the migration of mesenchymal stem cells isolated and cultured from bone marrow;5) activators containing HMGB1 which allows the migration of bone marrow mesenchymal stem cells can be conveniently purified from several organ extracts including skin, brain, and heart;6) activators which allow the migration of bone marrow mesenchymal stem cells can be conveniently extracted from cultured cells; and7) a heparin-column purified fraction of skin extract mobilizes a large amount of bone marrow-derived cells in case of brain injury.

Provided are: a skin-protecting agent and an external preparation for skin protection, which not only synergistically work in combination with a plant polysaccharide but also has an effect of promoting the production of glycerol derived from Staphylococcus epidermidis, which is a skin resident bacterium useful on the skin, and the production of antimicrobial peptides derived from skin epidermal keratinocytes and exhibits excellent effect of improving the touch feeling when applied to the skin. More specifically, the present invention provides a skin protective agent and an external preparationfor skin protection, which comprise a lactic acid bacterium belonging to the genus enterococcus.

A wound healing composition comprising an amount of heat shock protein effective to promote wound healing and a method thereof to apply the composition. A preferred heat shock protein is either full-length hsp90α or the middle domain plus the charged sequence of hsp90α. The composition is topically applied to skin wounds, covering the outer surface of the wound. The heat shock protein acts by promoting migration of both human epidermal keratinocyte and dermal fibroblasts to the wound in order to close, heal, and remodel the wound.

Provided are methods of treating and / or preventing dermatological disorders. Provided are methods of reducing skin inflammation, reducing pain, and / or reducing itch in a subject in need thereof. The methods may include administering to the subject an effective amount of a TRPV4 inhibitor. Further provided are compositions including a TRPV4 inhibitor compound in combination with a carrier, vehicle, or diluent that is suitable for topical application. Further provided is a transgenic mouse whose genome includes deletions of the Trpv4 gene in keratinocytes of the epidermis, wherein said transgenic mouse is a knockout for the Trpv4 gene in keratinocytes of the epidermis following keratinocyte-specific activation and expression of a site-specific recombination enzyme.

The present invention relates to a method for determining the state of epidermal differentiation of a human keratinocyte in a human epithelium sample, said method including determining the level of expression of at least one microRNA selected from among hsa-miR-455-3p, hsa-miR-141, hsa-miR-148a, hsa-miR-182, hsa-miR-224, hsa-miR-26a, hsa-miR-26b, hsa-miR-361-5p, hsa-miR-425, hsa-miR-92b, hsa-let-7i, hsa-miR-22, hsa-miR-221, hsa-miR-222, hsa-miR-29a, hsa-miR-29b, hsa-miR-663, hsa-miR-30a, and hsa-miR-30c within said keratinocyte, and comparing the level of expression of the microRNA(s) selected at the mean level of expression of the microRNA(s) selected in the undifferentiated keratinocytes or in differentiated epidermal keratinocytes, preferably from the same strain or the same population. The present invention also relates to various uses of the method for determining the state of epidermal differentiation, as well as to pharmaceutical or cosmetic compositions.

A wound healing composition comprising an amount of heat shock protein effective to promote wound healing and a method thereof to apply the composition. A preferred heat shock protein is either full-length hsp90α or the middle domain plus the charged sequence of hsp90α. The composition is topically applied to skin wounds, covering the outer surface of the wound. The heat shock protein acts by promoting migration of both human epidermal keratinocyte and dermal fibroblasts to the wound in order to close, heal, and remodel the wound.

The present inventors assessed the possibility that bone marrow-derived cells are mobilized to the grafted skin from nonskin tissues and contribute to skin tissue regeneration during the engraftment of grafted skin on biological tissues. As a result, the present inventors for the first time in the world demonstrated that:(1) a large number of bone marrow-derived cells are mobilized to grafted skin;(2) mobilized bone marrow-derived cells differentiate into any of dermal fibroblasts, adipocytes, muscle cells, vascular endothelial cells, and epidermal keratinocytes in grafted skin, and thus mobilized bone marrow-derived cells include bone marrow-derived mesenchymal stem cells;(3) S100A8 and S100A9 released from necrotic tissues of grafted skin are responsible for mobilizing bone marrow-derived mesenchymal stem cells to the grafted skin from peripheral blood; and(4) purified S100A8 and S100A9 promote the migration of mesenchymal stem cells isolated / cultured from the bone marrow.

An object of the invention is to provide artificial skin that does not contain any animal-derived material or pathogen and has excellent biocompatibility. The invention provides, as a solution means, a method for producing artificial skin comprising the steps of: (A) forming a dermal layer by solidifying a mixture of dermal fibroblasts and a peptide hydrogel having a fibrous structure; and (B) forming an epidermal layer by seeding skin keratinocytes onto the dermal layer obtained in Step (A), and culturing the epidermal keratinocytes.

The invention relates to a cosmetic composition in the technical field of daily chemical products, in particular to an anti-blue-light cosmetic composition and a preparation method thereof. The composition is mainly prepared from, by mass, 0.1-8 parts of a citrus extract, 0.1-4 parts of a lespedeza capitata leaf / stem extract, 0.1-4 parts of cerium oxide, 0.1-8 parts of a buddleja officinalis extract, 0.1-10 parts of a hedychium coronarium root extract and 0.1-8 parts of a rutin compound. The anti-blue-light cosmetic composition can brighten the skin, reduce the oxidative damage to the skin caused by blue light, increase the survival quantity of epidermal keratinocytes under the UV radiation, maintain the integrity of the skin function, reduce the chronic mild inflammation of light aging, and provide a skin protection effect by reducing free radicals produced by the induced oxidative stress, so that the generation amount of active oxygen ROS declines to improve local microcirculation and play a vasoconstriction role, thereby controlling the fatigue signs caused by direct blue light irradiation, keeping the skin in balance of circadian rhythms, and keeping the skin in a health status.

Epidermal keratinocytes not having magnetic fine particles incorporated therein and epidermal keratinocytes having magnetic fine particles incorporated therein are seeded in a 24-well ultralow adhesion plate. A neodymium magnet is disposed outside the well bottoms, and the cells are cultured for one day. As a result, control epidermal keratinocytes not having magnetic fine particles incorporated therein do not adhere to the well bottoms, resulting in non-formation of undifferentiated cell sheet. By contrast, epidermal keratinocytes having magnetic fine particles incorporated therein, while being attracted to the well bottoms by magnetic force, form a multilayered cell sheet. This cell sheet can be easily recovered by removing the neodymium magnet disposed outside the well bottoms, causing a magnet having a hydrophilic film stuck thereto to come close to the cell sheet from upside and attract it through the hydrophilic film, and updrawing them.

The invention provides a preparation method and application of a coated yeast fermentation product, and provides a preparation method of a coated yeast fermentation product. The method comprises the following steps: 1, preparing a culture medium: using a YPD culture medium as a primary seed culture medium, wherein the fermentation expression medium is a BSM medium. 2, performing fermentation expression. The invention also discloses application of the coated yeast fermentation product. The coated yeast fermentation product is a natural fermentation yeast product, and is rich in vitamins, essential amino acids, enzymes and yeast peptides. The coated yeast fermentation product filtrate can increase the expression of sericin and prevent the reduction of serine protein tyrosine mediated sericin, and the coated yeast fermentation product filtrate can increase the expression of caspase-14 in epidermal keratinocytes in a 3D model. The product can resist bacteria and diminish inflammation, promote wound healing, repair skin barriers and improve skin and mucous membrane immunity.

The present invention provides a novel DNA microarray chip that can be used for simultaneous testing of transcriptional responses to cutaneous stressors in the context of neuro-endocrine-immune functions of the skin. The transcriptional responses to ultraviolet radiation in epidermal keratinocytes were tested using such microarray chip containing more than 700 neuro-endocrine-immune related genes. The gene expression pattern was non-random and time dependent; it included increased expression of genes involved in water and salt balance, prostaglandin synthesis, keratinocyte differentiation as well as genes coding for stress effectors, cytokines and metalloproteinases. In contrast, expression was decreased for genes coding for growth factors and their receptors, and for elements of extracellular matrix. This stochastic pattern suggests that transcriptional responses are coordinated and aimed at preservation of epidermal barrier function, prevention of early carcinogenic events and remodeling of extracellular matrix.

The invention relates to a facial cleanser which comprises a decontamination component composed of saponaria officinalis extract and sapindus mukurossi extract, an exfoliating component composed of papaya extract, an oil-control component composed of impatiens balsamina extract, an acne-removing component composed of radices stemonae japonicae extract and a moisturizing component composed of tremella polysaccharides and hyaluronic acid. The invention further relates to a method for preparing the facial cleanser. According to the facial cleanser, any chemically synthetic surfactant is not added, the saponaria officinalis extract and sapindus mukurossi extract provide proper cleaning power, and the facial cleanser is mild and non-irritating. The papaya extract contributes to removing epidermal keratinocytes, enhancing the metabolism and making the skin tender; the impatiens balsamina extract is capable of inhibiting excessive secretion of skin oil, the radices stemonae japonicae extract is capable of inhibiting growth of mites, and when the impatiens balsamina extract and the radices stemonae japonicae extract are matched with each other, the product achieves excellent effects of controlling oil and inhibiting acnes; and a moisturizing system composed of the tremella polysaccharides and hyaluronic acid has high moisturizing ability and makes the skin comfortable.

The invention discloses a construction method for an in vitro skin test model containing melanocytes. The construction method comprises the following steps: using a culture medium for human epidermal keratinocytes as a basic liquid, and respectively preparing a culture medium a and a culture medium b; respectively preparing the keratinocytes and the melanocytes into cell suspensions by adopting the culture medium a, respectively and uniformly inoculating into a Transwell chamber, then adding the culture medium a, carrying out immersed culture for 1-5 days in a 5% CO2 incubator at the temperature of 37 DEG C, and forming an epidermal layer structure containing the melanocytes; placing on the surface of a culture support in a culture dish, adding the culture medium b, carrying out gas-liquid level culture, putting into the 5% CO2 incubator at the temperature of 37 DEG C and culturing for 5-15 days, so that the skin test model containing the melanocytes is obtained. The construction method disclosed by the invention has the advantages that model stability is high, the melanocytes are more sensitive to external stimulation, the model can be accurately used for detection on safety and efficacy assessment of cosmetics, raw material cost is low, production cycle is short, stability is good, and the model can be produced in quantity.

The invention disclosed relates to the culturing of mammalian e.g. human cells, and in particular to the culturing and expansion of substantially undifferentiated human epidermal keratinocytes exhibiting stem cell-like characteristics, and in co-culture with human dermal fibroblasts utilizing a low calcium serum free and animal by-product free medium derived from a commercially available medium. The medium consists of a commercially available basal medium, and a single fibroblast growth factor (FGF) or a mimic thereof e.g. FGF7 / KGF. A method is also disclosed for treating mammalian e.g. human skin wound injuries, by applying to the wound an effective amount of substantially pure mammalian e.g. human epidermal stem cell-like keratinocytes in a substantially undifferentiated state, and optionally additionally, dermal fibroblasts e.g. human dermal fibroblasts. Both cell types can be grown separately or in a co-culture for application purposes.

The present invention concerns a process for preparation of a biomass comprising one or a more plantaricins A, N or K in association with the lactic acid bacteria used for the preparation and uses thereof in order to stimulate the barrier function of intestinal cells or human epidermal keratinocytes

Studies have been made on a possibility that a bone-marrow-derived cell is recruited from an extracutaneous tissue into a skin graft during the process of adhesion of the skin graft to a biological tissue and therefore contributes to the regeneration of a skin tissue. As a result, the following facts 1) to 4) are found: 1) a large quantity of bone-marrow-derived cells are recruited into a skin graft; 2) the recruited bone-marrow-derived cells are differentiated into all of dermal fibroblasts, adipocytes, muscle cells, vascular endothelial cells and epidermal keratinocytes in the skin graft, and bone-marrow-derived mesenchymal stem cells are contained in the recruited bone-marrow-derived cells; 3) the substances which recruit the bone-marrow-derived mesenchymal stem cells from the peripheral blood into the skin graft are S100A8 and S100A9 which are released from a dead tissue in the skin graft; and 4) purified S100A8 and S100A9 can promote the migration of a mesenchymal stem cell which has been separated from a bone marrow and cultured.

The invention discloses a Chinese medicinal herb scrub exfoliating cream, wherein the constituents of the Chinese medicinal herb scrub exfoliating cream are as follows: a pawpaw extract, glycyrrhiza essence, an oat extract, glycerol, stearin, glycerol, metronidazole, deionized water, white oil and vitamin E. The scrub exfoliating cream provided by the invention takes the herbal essences as the main raw materials and has the effects of softening epidermal keratinocytes, dredging the pores, improving rough and dark skin, nourishing the skin and changing the colour of skin and the like. The main constituents of the Chinese medicinal herb scrub exfoliating cream are herbal plants with the effects of skin protection and beauty maintenance recorded in the Compendium Of Materia Medica, such as the pawpaw, the glycyrrhiza and the oat. The Chinese medicinal herb scrub exfoliating cream is an ideal traditional Chinese medicine conditioning product, which is suitable for various skins due to the fact that the Chinese medicinal herb scrub exfoliating cream is free from side effects after being utilized.

The invention discloses the culturing, screening and amplification technology for keratinocytes. The bottlenecks are always hot spots and difficulty in biological cell field research. At present, separation and purification are conducted through basilar membrane significant adhesion characteristics, and the keratinocytes are obtained through a three-dimensional culturing and amplification means. The technology is suitable for depending on amplified anchorage-dependent cells and providing a three-dimensional high-simulation in-vivo cell extracellular matrix system for screening and separation of target cell levels. The in-vivo attachment environment is highly simulated, and a novel biological cell culturing technology for culturing adult (embryo skin) skin cells is provided. Adhering, screened and cultured target keratinocytes in the environment of high-simulation substrates have high passage capacity and multiplication capacity and can form a cortex structure under the in-vitro environment. Immunogenicity cells are screened out to safely obtain immune escape, transplanting time is prolonged, tissue engineering clinical application is improved, wound healing is promoted, skin diseases are treated, and a clinical treatment-grade cell solution with absolute advantages is provided.

The invention discloses a rat dermal mesenchymal stem cell culture method. The method comprises the steps of digesting skin tissue and keeping the skin tissue for a whole night; separating epidermis and dermis of the skin tissue; sticking a dermis tissue block in a cell culture dish and adhering in an inverted incubator at 37 DEG C for half an hour, and cultivating the tissue block through low-sugar DMEM and 10% of FBS; implementing conventional pancreatin digestion subculture when the dish is full of cells cultivated from the dermis tissue block to obtain dermal mesenchymal stem cells. The method disclosed by the invention is a novel method combining a skin tissue block adherent method and an enzyme digestion method, not only effectively avoids epidermal keratinocyte pollution caused by the skin tissue block adherent method but also avoids influence of a long-time pancreatic function on a cell growth state in the enzyme digestion method. The culture method disclosed by the invention provides possibility for further research on effects of mesenchymal stem cells in the skin tissue on skin development, growth and damage repair.

The invention discloses a photoprotective plant extract composition and application thereof. The photoprotective plant extract composition comprises the following components: a Feronia limonia Swingleextract, a butterflybush flower extract, a rhodiola rosea extract and a dried meat floss extract. The photoprotective plant extract composition effectively absorbs ultraviolet light and blue light through the synergistic effect of all the components, has excellent anti-ultraviolet and anti-blue-light effects, can effectively inhibit ultraviolet, blue light and red light induction from causing active oxygen increase, and maintains the activity of human epidermal keratinocytes and human dermal cells, so that the generation of collagen is promoted, the generation of free radicals is effectivelyresisted, a comprehensive photoprotective effect can be achieved, and the composition is beneficial to repairing the damage of radiation to the skin and relieving the skin sensitivity problem.

The invention belongs to the technical field of medicine and discloses the application of diphenylheptane compounds in the preparation of medicines or food for preventing and treating psoriasis. The diphenylheptane compound has the structure shown in formula I: Wherein, the 4-5 position of the diphenylheptane compound is a single bond or a double bond, and the 6-7 position is a single bond or a double bond; R 1 is double bond oxygen or acetoxy; R 2 is hydrogen, hydroxy or methoxy. The diphenylheptane compound of the invention has inhibitory effect on the proliferation of human skin T cell lymphoma cell line HH cells and human immortalized epidermal keratinogenic cell line HaCaT cells, and can be applied to the preparation of medicines or food for preventing and treating psoriasis.

The present invention addresses the problem of providing a new therapeutic agent that is effective against diseases involving stratified squamous epithelial cells such as dry eye syndrome. The presentinvention is a stratified squamous epithelial cell normal differentiation and maturation promoting agent that comprises secretions of mesenchymal stem cells. It is preferable that the secretions notinclude animal serum, that the mesenchymal stem cells be adipose-derived mesenchymal stem cells, umbilical cord-derived mesenchymal stem cells, or bone marrow-derived mesenchymal stem cells, and thatthe stratified squamous epithelial cells be at least one selected from the group consisting of corneal epithelial cells, conjunctival epithelial cells, epidermal keratinocytes, oral epithelial cells,epiglottic epithelial cells, esophageal epithelial cells, vaginal epithelial cells, vocal fold epithelial cells, nasal epithelial cells, and nasal vestibular epithelial cells.

The invention relates to the field of cell culture, and particularly discloses a method for constructing a human dermal fibroblast in-vitro thermal damage model and application of the method. The method mainly comprises the following steps: 1) separating and culturing human dermal fibroblasts; (2) purifying the human dermal fibroblasts: digesting primary culture cells for 1-2 minutes by using 0.05% pancreatin / EDTA according to the difference of the sensitivities of the dermal fibroblasts and the epidermal keratinocytes to pancreatin, wherein dermal fibroblasts are digested; the cells are transferred to a third generation by using the method, so that the dermal fibroblasts with the purity of 100% can be obtained; and 3) constructing the dermal fibroblast in-vitro thermal damage model: placing a culture plate inoculated with the dermal fibroblasts in a 37 DEG C water bath kettle, and carrying out heat treatment for 50 minutes to obtain the dermal fibroblast thermal damage model.

The invention relates to an exfoliating skin cream which is composed of a wormwood extract, a licorice essence, an oat essence, pseudo-ginseng, glycerol stearate, glycerin, deionized water, white oil, and vitamin A. According to the exfoliating skin cream, the wormwood extract is adopted as a main raw material. The exfoliating skin cream has the functions of epidermal keratinocyte softening, dead skin removing, pore unblocking, rough and dull skin ameliorating, skin nourishing, skin tone improving, and the like. The main components wormwood, licorice, and oat are skin-protecting and skin-nourishing herbal plants recorded in Compendium of Materia Medica. The exfoliating skin cream is a traditional Chinese medicine conditioning product causing no side effect. The exfoliating skin cream is suitable for various skins.