23 results about "Epidermal keratinocyte" patented technology

The invention discloses a pseudo-ginseng exosome-like nano vesicle loaded with an epidermal growth factor / application of the pseudo-ginseng exosome-like nano vesicle in wound healing. The research shows that not all exosome-like nano-vesicles from Chinese herbal medicines capable of promoting wound healing have the effect of promoting wound healing, the action mechanism of the pseudo-ginseng exosome-like nano-vesicles in wound healing is further explored, and the research shows that PNVs can promote the proliferation and migration of an epidermal keratinocyte line, so that the PNVs can be used for promoting the healing of the wound. The treatment can be realized by regulating and controlling the activity of immune cells to promote cell proliferation and migration, and the wound healing effect of the EGF-loaded EGF-coated PNVs is remarkably enhanced, so that the EGF-loaded EGF-coated PNVs have a synergistic effect. The invention not only provides a theoretical basis for deeply researching PELNVs, but also provides a new method and a new idea for treating acute wounds.

To provide actin reorganization inhibitors and phagocytosis inhibitors, which inhibit the actin reorganization and induction of phagocytosis in epidermal keratinocytes by contacting the epidermal keratinocytes with a fibroblast conditioned medium; to provide stain formation inhibitors containing the inhibitor; and to provide external skin preparations containing the inhibitor.SOLUTION: Provided is an inhibitor that inhibits the actin reorganization and induction of phagocytosis in epidermal keratinocytes by contacting the epidermal keratinocytes with a fibroblast conditioned medium, and contains a specific plant extract or a specific compound as an active ingredient. Also provided are a spot formation inhibitor composed of the inhibitor, and a skin external preparation containing the inhibitor.SELECTED DRAWING: None

To provide agents for promoting epidermal keratinocyte growth, filaggrin mRNA expression, serine palmitoyltransferase mRNA expression, involucrin expression or ATP production by finding substances having excellent activity in promoting epidermal keratinocyte growth, filaggrin mRNA expression, serine palmitoyltransferase mRNA expression, involucrin expression or ATP production.SOLUTION: An epidermal keratinocyte growth promoting agent of the invention comprises one or more effective ingredients selected from the group consisting of 2-ketoglutaric acid, 2-isopropyl malate, inosine 5'-monophosphoric acid, succinic acid, malic acid and pyruvic acid. Also, a filaggrin mRNA expression promotor, a serine palmitoyltransferase mRNA expression promotor, an involucrin expression promotor of the invention comprise, as an effective ingredient, 2-ketoglutaric acid. Further an ATP production promotor of the invention comprises 2-isopropyl malate as an effective ingredient.SELECTED DRAWING: None

[Summary] [Problems] The main purpose of this invention is to provide a technique for inducing an epidermal keratinocyte that can produce a three-dimensional cultured skin model with a multi-layered horny cell layer, from a pluripotent stem cell. [Solution] The present invention is a method for inducing differentiation from a pluripotent stem cell to an epidermal keratinocyte, using a non-detached cell after being treated with a cell detaching agent in the inducing step from the pluripotent stem cell, wherein when applying the epidermal keratinocyte to a three-dimensional cultured skin model, a three-dimensional cultured skin model in which the horny cell layer is multi-layered is obtained.

The invention relates to the technical field of cosmetics, in particular to a compound active peptide with skin anti-aging, anti-inflammatory and soothing functions and application of the compound active peptide. The compound active peptide is prepared from acetyl hexapeptide-1, palmitoyl tripeptide-5, acetyl dipeptide-1 cetyl ester, copper peptide and decarboxylated carnosine hydrochloride. The compound peptide acts on dermal fibroblasts, so that expression of p16 and p21 genes is reduced, and cell cycle arrest is improved; the compound acts on epidermal keratinocytes and dermal fibroblasts and inhibits COX-2 gene expression; the compound peptide acts on epidermal keratinocytes and dermal fibroblasts, and the ROS level is reduced; the compound peptide acts on PC12 cells, inhibits secretion of acetylcholine, blocks transmission of neural signals, and plays a role in immediate wrinkle removal.

To provide a screening method for novel skin disease inhibitors.SOLUTION: A comparison is made between gene expression 1 in human epidermal keratinocytes after a first culture step of culturing the human epidermal keratinocytes in the presence of Corynebacterium bacteria and gene expression 2 in human epidermal keratinocytes after a second culture step of culturing the human epidermal keratinocytes in the presence of Corynebacterium bacteria and the test substance. The comparison enables evaluation of the skin disease inhibitory effect of the test substance in the human epidermal keratinocytes.SELECTED DRAWING: None

The present invention relates to a cosmetic skincare composition, preferably anti-aging, for improving the superficial visual appearance of the skin, giving it a more youthful appearance, comprising an extract of Primrose and an extract of dedifferentiated cells of Pale Iris. The invention also relates to the use of such a combination to inhibit the expression of MMP-2 and MMP-9 in epidermal keratinocytes.

The invention discloses an anti-wrinkle composition containing a camellia japonica extract and capable of promoting cell energy and an application of the anti-wrinkle composition. The anti-wrinkle composition is prepared from the following components in percentage by mass: 5 to 20 percent of camellia japonica extract, 7.4 to 7.6 percent of flaxseed oil, 0.14 to 0.16 percent of sorbitan trioleate, 0.005 to 0.007 percent of celery seed extract, 0.004 to 0.006 percent of crithmum maritimum extract, 0.003 to 0.004 percent of flaxseed extract and the balance of caprylic / capric triglyceride which is supplemented to 100 percent. The invention further discloses application of the anti-wrinkle composition in promoting synthesis of NAD < + > and ATP in dermal fibroblasts and / or epidermal keratinocytes and / or SIRT3 gene expression and application of the anti-wrinkle composition in preparing cosmetics or skin care products with anti-wrinkle and tightening effects. The composition disclosed by the invention is an oil-soluble composition, is easier to absorb, and can improve the mitochondrial activity through synergistic interaction.

The invention provides an HPV (human papillomavirus) infected human epidermal keratinocyte model and a construction method and application thereof, and relates to the technical field of molecular biology, an HPV genome is integrated to a KRT5 site of genome DNA (deoxyribonucleic acid) of human epidermal keratinocytes to obtain the HPV infected human keratinocyte model, stable integration and expression of an HPV whole genome are successfully realized, and the HPV infected human keratinocyte model is obtained. The position effect caused by random insertion is avoided, and the genetic stability is ensured; the model has the synergistic expression function of HPV early and late genes, maintains the time sequence of virus gene expression, realizes HPV virus particle assembly, and simulates the complete life cycle of HPV. The technical problem that in the prior art, an infection model cannot simulate the complete life cycle of the HPV is solved.

The present invention addresses the problem of providing a novel therapeutic agent that is effective against diseases involving stratified squamous epithelial cells such as dry eye syndrome. The present invention is an agent for promoting normal differentiation / maturation of stratified squamous epithelial cells, which comprises a secretion of mesenchymal stem cells. It is preferable that the secretions not include animal serum, that the mesenchymal stem cells be adipose-derived mesenchymal stem cells, umbilical cord-derived mesenchymal stem cells, or bone marrow-derived mesenchymal stem cells, and that the stratified squamous epithelial cells be at least one selected from the group consisting of corneal epithelial cells, conjunctival epithelial cells, epidermal keratinocytes, oral epithelial cells, epiglottic epithelial cells, esophageal epithelial cells, vaginal epithelial cells, vocal fold epithelial cells, nasal epithelial cells, and nasal vestibular epithelial cells.

To discover, from among compositions of natural origin having high safety, ones having filaggrin mRNA expression promoting action, involucrin mRNA expression promoting action, and transglutaminase-1 mRNA expression promoting action, and provide a filaggrin mRNA expression promoter, an involucrin mRNA expression promoter, and a transglutaminase-1 mRNA expression promoter containing the same as an active ingredient.SOLUTION: A filaggrin mRNA expression promoter, an involucrin mRNA expression promoter, and a transglutaminase-1 mRNA expression promoter, which are used to promote the expression of filaggrin mRNA, involucrin mRNA, and transglutaminase-1 mRNA in epidermal keratinocytes, are made to contain, as an active ingredient, a water extract from the leaf pulp portion of Aloe vera and / or a hydrophilic organic solvent extract from the leaf pulp portion of Aloe vera.SELECTED DRAWING: None

The invention relates to a small molecule compound and application thereof in preparation of a medicine for treating 24p3R-mediated diseases. The invention relates to an application of small molecule compounds 5509605 and 5318411 or pharmaceutically acceptable salts thereof in preparation of medicines for treating diseases mediated by 24p3R (24p3R), in particular to an application of the small molecule compounds 5509605 and 5318411 in preparation of medicines for treating diseases mediated by 24p3R. According to the technical scheme, the core mechanism of the compound in treatment of 24p3R-mediated skin inflammatory diseases is disclosed for the first time by screening and verifying the targeted regulation effect of a specific small molecule compound on a 24p3R receptor. The small molecule drug can be specifically combined with an active site of a 24p3R receptor protein, and by blocking the combination of 24p3R and LCN2, the activation of an LCN2-SREBP2-NLRC4 signal axis is inhibited, and the expression of proinflammatory factors such as IL1beta, CXCL1 and CCL20 is reduced, so that the pathological proliferation and immune cell infiltration of psoriasis characteristic epidermal keratinocytes are blocked at the molecular level.

The anti-inflammatory composition promotes proliferation of macrophage and suppresses inflammation caused by inflammatory cytokine and / or NO synthase, and promotes proliferation of human fibroblast or human epidermal keratinocyte and promotes synthesis of hyaluronic acid in epidermis and dermis. To provide a skin-beautifying and promoting composition which keeps the tension of the skin, strengthens the bonding force of the skin, improves the barrier function of the skin, enhances the strength and water-retaining function of the skin surface, or promotes the anti-aging of the skin, to provide a hair-growing composition which promotes the proliferation of human epidermal keratinocyte cells, improves the strength of the skin surface, and promotes hair growth, and to provide cosmetic compositions thereof.SOLUTION: The composition comprises a high-pressure treated product of a mixture of sturgeon eggs and 1, 3-butylene glycol.SELECTED DRAWING: Figure 3

The present invention discloses the use of compound G414-0147 in the preparation of a drug for promoting skin wound healing, wherein the molecular formula of compound G414-0147 is C 25 H 30 FN7, with the structural formula #imgabs0#, is a small molecule drug proposed in the present invention for use in preparing a drug that promotes skin wound healing. The drug, in particular, contains compound G414-0147 or a pharmaceutically acceptable salt thereof as an active ingredient. The drug promotes skin wound healing by promoting epidermal keratinocyte migration. The drug is highly safe, stable, can treat large-area skin wounds, and has low synthesis cost. This drug provides a new approach for the clinical treatment of skin wound healing and has important clinical application value.

The invention provides a composition for promoting skin microcirculation, makeup removing cream and a preparation method of the makeup removing cream, and relates to the technical field of skin care products. The composition for promoting skin microcirculation comprises an ingredient for inducing vasodilation, an ingredient for improving vascular tolerance, an ingredient for reducing free heme and an ingredient for relieving perceptual pressure, and skin care is realized on the basis of improving forward emotion perception and accelerating blood circulation. Compared with a traditional single microcirculation path, for example, human epidermal keratinocyte and normal vascular endothelial cells are purely promoted to synthesize nitric oxide, a nitric oxide mediated negative feedback loop is activated to stimulate smooth muscle mediated vasodilation, and blood circulation is not obviously promoted. According to the application, the preferable raw material design comprises the steps of inducing vasodilation, preventing a large amount of hemoglobin from permeating out of the skin and then degrading permeated free heme to realize a multi-channel closed loop to promote blood microcirculation, and active matters capable of regulating endokephin secretion are matched to further relax facial muscles and relieve the pressure skin.

The invention belongs to the field of nano medicine, and particularly relates to a dendritic mesoporous silicon nano material loaded with glucose oxidase as well as a preparation method and application of the dendritic mesoporous silicon nano material. Glucose oxidase is loaded on the dendritic mesoporous silicon nano-material by utilizing the electrostatic interaction and pore adsorption effect between the dendritic mesoporous silicon nano-material with negative charges and glucose oxidase with positive charges, and the glucose oxidase is loaded on the dendritic mesoporous silicon nano-material by adjusting the dosage of the glucose oxidase and the dendritic mesoporous silicon. The dendritic mesoporous silicon nano material (DMSN (at) GOx) loaded with the glucose oxidase is obtained. The DMSN-coated GOx can effectively reduce the glucose level and the pH value of the psoriasis site, further reduce the chemotactic factor generation level of keratinocytes, cut off the bridge with immune cells, inhibit the inflammatory factor level of an inflammatory region, effectively relieve the abnormal hyperplasia of the keratinocytes of an epidermal layer and the infiltration of immune cells of a corium layer, and improve the psoriasis treatment effect. The purpose of treating psoriasis is achieved, but no obvious toxicity is caused to normal tissues.

The present invention relates to: a composition for regenerating or repairing skin damage and / or promoting epidermal cell differentiation, comprising calcium lactate as an active ingredient; and a method therefor, wherein the skin damage includes that caused by ultraviolet rays. The present invention induces differentiation of epidermal keratinocytes so as to form a biological barrier and allows the skin to maintain moisture on its own, and, as a temporary effect, plays a role in protecting the skin from UV by forming nanoparticles on the surface thereof.

The invention belongs to the field of drugs for inflammatory dermatosis, and discloses an application of myrcene in preparation of drugs for inflammatory dermatosis, which comprises the following steps: combining a mouse AD model (DNCB) with human epidermal keratinocytes (NHEK) and mouse macrophages (RAW264.7); the improvement effect of the MYR is evaluated by adopting histology and immunofluorescence technologies; by integrating transcriptomics and proteomics analysis, the molecular mechanism of MYR for relieving AD symptoms is clarified, and the causal relationship is verified by using a pharmacological inhibitor. The conclusion of the invention is as follows: Myrcene alleviates AD sample inflammation by inhibiting FAK / Src-YAP / TAZ axis. A repeatable mechanism framework is established by the data, and the natural monoterpenoids are associated with biomechanical-nuclear signal transduction and innate immune regulation in AD.

Traditional research focusing on protein synthesis has focused on the total amount of protein, and therefore requires components that either suppress the decrease in the amount of newly synthesized protein or promote its synthesis. [Solution] Focusing on the synthesis of new proteins, and utilizing a method for evaluating the amount of new protein synthesis in dermal fibroblasts and epidermal keratinocytes, neem leaf extract and kudzu extract are provided as inhibitors of the decrease in the amount of new protein synthesis or as promoters of new protein synthesis.

[Problems] The main purpose of this invention is to provide a technique for inducing an epidermal keratinocyte that can produce a three-dimensional cultured skin model with a multi-layered horny cell layer, from a pluripotent stem cell.[Solution] The present invention is a method for inducing differentiation from a pluripotent stem cell to an epidermal keratinocyte, using a non-detached cell after being treated with a cell detaching agent in the inducing step from the pluripotent stem cell, wherein when applying the epidermal keratinocyte to a three-dimensional cultured skin model, a three-dimensional cultured skin model in which the horny cell layer is multi-layered is obtained.[Selected Drawing] none.

The purpose of the present invention is to provide a novel material that promotes the production of collagen and / or hyaluronic acid and / or activates human epidermal keratinocytes. A lactic acid bacterium belonging to the genus Fructobacillus, a culture of the lactic acid bacterium, a culture supernatant of the lactic acid bacterium, and / or an extract of the lactic acid bacterium are useful as active ingredient of a collagen production promoter, a hyaluronic acid production promoter, and an epidermal keratinocyte activator.

Skin cancer is the most common form of malignancy in humans, with significant associated morbidity and cost, and steadily increasing incidence. Since it is now well established that UV-damaged epidermal keratinocytes in intact skin can harbor on the order of 5 mutations / Mb, this raises the possibility of using this mutational burden as a dosimeter of UV-induced damage and potentially skin cancer risk. In particular, sampling normal skin for biomarkers of UV exposure can lead to better understanding of the genomic alterations associated with initiation of skin cancer, which ultimately will aid in stratifying risk of skin cancer development, determination of effectiveness of current prevention methods, and better targeted therapies. Disclosed herein are devices and systems for interrogation of normal non-lesional skin to extract epidermal DNA for analysis. This approach can be generalized to any epithelial surface including in the oral cavity, gastrointestinal, or genitourinary tracts.

The invention relates to a skin care composition for external use. The skin care composition for external use comprises nicotinamide and one or more resveratrol salicylate. The composition increases the level of energy in epidermal keratinocytes while mitigating oxidative damage associated with increased ATP production due to administration of nicotinamide. The performance of AQP-3, CLDN-1, and CLDN-4 is also increased, thereby improving barrier function in the skin and increasing moisture retention. Preferably, the composition is used as a mixture or emulsion in the form of cream, lotion, essence or gel.