66 results about "Epidermal keratinocyte" patented technology

The present invention discloses a nano silver biological bionic dressing and its preparation method. Said invention utilizes nano technology and self-assembling technology to assemble nano silver particle layer on the chitosan film. Its preparation includes the following steps: (1) preparation of nano silver particles: preparing silver nitrate (Ag NO3) solution, placing said solution in a container, stirring, heating and boiling, then drop-adding sodium citrate (Na3 C6 H5 O7 2H2O) solution, reacting for a certain time, then naturally cooling; (2) preparation of chitosan film: using acetic acid solution to dissolve chitosan, suction-filtering, pouring the suction-filtered solution into culture dish, drying to form film, using sodium hydroxide (No OH) to make treatment, finally using running water to rinse the film; and (3) preparation of nano silver biological bionic dressing: soaking the chitosan film in the nano silver particle solution, utilizing self-assembling technique to make nano silver particles be assembled on the chitosan film so as to obtain the invented product.

Epidermal keratinocytes not incorporating magnetic particles therein and epidermal keratinocytes incorporating magnetic particles therein were seeded in a 24-well ultra-low-attachment plate, and cultured for one day after a neodymium magnet was placed outside the bottom of the well. As a result, control epidermal keratinocytes not incorporating magnetic particles therein did not adhere to the bottom of the well and did not form an undifferentiated cell sheet. On the contrary, epidermal keratinocytes incorporating magnetic particles therein, while being attracted to the bottom of the well by magnetic force, formed a multilayered cell sheet. This cell sheet could be easily recovered by removing the neodymium magnet placed outside the bottom of the well, then bringing a magnet having a hydrophilic film attached thereto close to the cell sheet from upside and to attract it via this hydrophilic film, and lifting it.

The present inventors revealed the following for the first time in the world:

• 1) a large amount of bone marrow-derived cells are mobilized to grafted skin;
• 2) the mobilized bone marrow-derived cells are differentiated into any of dermal fibroblasts, adipocytes, muscle cells, vascular endothelial cells, and epidermal keratinocytes in the grafted skin, and the mobilized bone marrow derived cells include bone marrow-derived mesenchymal stem cells;
• 3) the factors which mobilize bone marrow-derived mesenchymal stem cells from peripheral blood to the grafted skin are HMGB1, HMGB2, and HMGB3 released from the necrosed tissue of recipient skin;
• 4) purified HMGB1, HMGB2, and HMGB3 promote the migration of mesenchymal stem cells isolated and cultured from bone marrow;
• 5) activators containing HMGB1 which allows the migration of bone marrow mesenchymal stem cells can be conveniently purified from several organ extracts including skin, brain, and heart;
• 6) activators which allow the migration of bone marrow mesenchymal stem cells can be conveniently extracted from cultured cells; and
• 7) a heparin-column purified fraction of skin extract mobilizes a large amount of bone marrow-derived cells in case of brain injury.

A wound healing composition comprising an amount of heat shock protein effective to promote wound healing and a method thereof to apply the composition. A preferred heat shock protein is either full-length hsp90α or the middle domain plus the charged sequence of hsp90α. The composition is topically applied to skin wounds, covering the outer surface of the wound. The heat shock protein acts by promoting migration of both human epidermal keratinocyte and dermal fibroblasts to the wound in order to close, heal, and remodel the wound.

Provided are methods of treating and/or preventing dermatological disorders. Provided are methods of reducing skin inflammation, reducing pain, and/or reducing itch in a subject in need thereof. The methods may include administering to the subject an effective amount of a TRPV4 inhibitor. Further provided are compositions including a TRPV4 inhibitor compound in combination with a carrier, vehicle, or diluent that is suitable for topical application. Further provided is a transgenic mouse whose genome includes deletions of the Trpv4 gene in keratinocytes of the epidermis, wherein said transgenic mouse is a knockout for the Trpv4 gene in keratinocytes of the epidermis following keratinocyte-specific activation and expression of a site-specific recombination enzyme.

The present invention relates to a method for determining the state of epidermal differentiation of a human keratinocyte in a human epithelium sample, said method including determining the level of expression of at least one microRNA selected from among hsa-miR-455-3p, hsa-miR-141, hsa-miR-148a, hsa-miR-182, hsa-miR-224, hsa-miR-26a, hsa-miR-26b, hsa-miR-361-5p, hsa-miR-425, hsa-miR-92b, hsa-let-7i, hsa-miR-22, hsa-miR-221, hsa-miR-222, hsa-miR-29a, hsa-miR-29b, hsa-miR-663, hsa-miR-30a, and hsa-miR-30c within said keratinocyte, and comparing the level of expression of the microRNA(s) selected at the mean level of expression of the microRNA(s) selected in the undifferentiated keratinocytes or in differentiated epidermal keratinocytes, preferably from the same strain or the same population. The present invention also relates to various uses of the method for determining the state of epidermal differentiation, as well as to pharmaceutical or cosmetic compositions.

The present inventors assessed the possibility that bone marrow-derived cells are mobilized to the grafted skin from nonskin tissues and contribute to skin tissue regeneration during the engraftment of grafted skin on biological tissues. As a result, the present inventors for the first time in the world demonstrated that:

(1) a large number of bone marrow-derived cells are mobilized to grafted skin;

(2) mobilized bone marrow-derived cells differentiate into any of dermal fibroblasts, adipocytes, muscle cells, vascular endothelial cells, and epidermal keratinocytes in grafted skin, and thus mobilized bone marrow-derived cells include bone marrow-derived mesenchymal stem cells;

(3) S100A8 and S100A9 released from necrotic tissues of grafted skin are responsible for mobilizing bone marrow-derived mesenchymal stem cells to the grafted skin from peripheral blood; and

(4) purified S100A8 and S100A9 promote the migration of mesenchymal stem cells isolated/cultured from the bone marrow.

An object of the invention is to provide artificial skin that does not contain any animal-derived material or pathogen and has excellent biocompatibility. The invention provides, as a solution means, a method for producing artificial skin comprising the steps of: (A) forming a dermal layer by solidifying a mixture of dermal fibroblasts and a peptide hydrogel having a fibrous structure; and (B) forming an epidermal layer by seeding skin keratinocytes onto the dermal layer obtained in Step (A), and culturing the epidermal keratinocytes.

The invention relates to a cosmetic composition in the technical field of daily chemical products, in particular to an anti-blue-light cosmetic composition and a preparation method thereof. The composition is mainly prepared from, by mass, 0.1-8 parts of a citrus extract, 0.1-4 parts of a lespedeza capitata leaf/stem extract, 0.1-4 parts of cerium oxide, 0.1-8 parts of a buddleja officinalis extract, 0.1-10 parts of a hedychium coronarium root extract and 0.1-8 parts of a rutin compound. The anti-blue-light cosmetic composition can brighten the skin, reduce the oxidative damage to the skin caused by blue light, increase the survival quantity of epidermal keratinocytes under the UV radiation, maintain the integrity of the skin function, reduce the chronic mild inflammation of light aging, and provide a skin protection effect by reducing free radicals produced by the induced oxidative stress, so that the generation amount of active oxygen ROS declines to improve local microcirculation and play a vasoconstriction role, thereby controlling the fatigue signs caused by direct blue light irradiation, keeping the skin in balance of circadian rhythms, and keeping the skin in a health status.

The present invention provides a novel DNA microarray chip that can be used for simultaneous testing of transcriptional responses to cutaneous stressors in the context of neuro-endocrine-immune functions of the skin. The transcriptional responses to ultraviolet radiation in epidermal keratinocytes were tested using such microarray chip containing more than 700 neuro-endocrine-immune related genes. The gene expression pattern was non-random and time dependent; it included increased expression of genes involved in water and salt balance, prostaglandin synthesis, keratinocyte differentiation as well as genes coding for stress effectors, cytokines and metalloproteinases. In contrast, expression was decreased for genes coding for growth factors and their receptors, and for elements of extracellular matrix. This stochastic pattern suggests that transcriptional responses are coordinated and aimed at preservation of epidermal barrier function, prevention of early carcinogenic events and remodeling of extracellular matrix.

The invention relates to a facial cleanser which comprises a decontamination component composed of saponaria officinalis extract and sapindus mukurossi extract, an exfoliating component composed of papaya extract, an oil-control component composed of impatiens balsamina extract, an acne-removing component composed of radices stemonae japonicae extract and a moisturizing component composed of tremella polysaccharides and hyaluronic acid. The invention further relates to a method for preparing the facial cleanser. According to the facial cleanser, any chemically synthetic surfactant is not added, the saponaria officinalis extract and sapindus mukurossi extract provide proper cleaning power, and the facial cleanser is mild and non-irritating. The papaya extract contributes to removing epidermal keratinocytes, enhancing the metabolism and making the skin tender; the impatiens balsamina extract is capable of inhibiting excessive secretion of skin oil, the radices stemonae japonicae extract is capable of inhibiting growth of mites, and when the impatiens balsamina extract and the radices stemonae japonicae extract are matched with each other, the product achieves excellent effects of controlling oil and inhibiting acnes; and a moisturizing system composed of the tremella polysaccharides and hyaluronic acid has high moisturizing ability and makes the skin comfortable.

The invention disclosed relates to the culturing of mammalian e.g. human cells, and in particular to the culturing and expansion of substantially undifferentiated human epidermal keratinocytes exhibiting stem cell-like characteristics, and in co-culture with human dermal fibroblasts utilizing a low calcium serum free and animal by-product free medium derived from a commercially available medium. The medium consists of a commercially available basal medium, and a single fibroblast growth factor (FGF) or a mimic thereof e.g. FGF7/KGF. A method is also disclosed for treating mammalian e.g. human skin wound injuries, by applying to the wound an effective amount of substantially pure mammalian e.g. human epidermal stem cell-like keratinocytes in a substantially undifferentiated state, and optionally additionally, dermal fibroblasts e.g. human dermal fibroblasts. Both cell types can be grown separately or in a co-culture for application purposes.

The present invention concerns a process for preparation of a biomass comprising one or a more plantaricins A, N or K in association with the lactic acid bacteria used for the preparation and uses thereof in order to stimulate the barrier function of intestinal cells or human epidermal keratinocytes

The invention discloses a Chinese medicinal herb scrub exfoliating cream, wherein the constituents of the Chinese medicinal herb scrub exfoliating cream are as follows: a pawpaw extract, glycyrrhiza essence, an oat extract, glycerol, stearin, glycerol, metronidazole, deionized water, white oil and vitamin E. The scrub exfoliating cream provided by the invention takes the herbal essences as the main raw materials and has the effects of softening epidermal keratinocytes, dredging the pores, improving rough and dark skin, nourishing the skin and changing the colour of skin and the like. The main constituents of the Chinese medicinal herb scrub exfoliating cream are herbal plants with the effects of skin protection and beauty maintenance recorded in the Compendium Of Materia Medica, such as the pawpaw, the glycyrrhiza and the oat. The Chinese medicinal herb scrub exfoliating cream is an ideal traditional Chinese medicine conditioning product, which is suitable for various skins due to the fact that the Chinese medicinal herb scrub exfoliating cream is free from side effects after being utilized.

The invention discloses a rat dermal mesenchymal stem cell culture method. The method comprises the steps of digesting skin tissue and keeping the skin tissue for a whole night; separating epidermis and dermis of the skin tissue; sticking a dermis tissue block in a cell culture dish and adhering in an inverted incubator at 37 DEG C for half an hour, and cultivating the tissue block through low-sugar DMEM and 10% of FBS; implementing conventional pancreatin digestion subculture when the dish is full of cells cultivated from the dermis tissue block to obtain dermal mesenchymal stem cells. The method disclosed by the invention is a novel method combining a skin tissue block adherent method and an enzyme digestion method, not only effectively avoids epidermal keratinocyte pollution caused by the skin tissue block adherent method but also avoids influence of a long-time pancreatic function on a cell growth state in the enzyme digestion method. The culture method disclosed by the invention provides possibility for further research on effects of mesenchymal stem cells in the skin tissue on skin development, growth and damage repair.

The invention belongs to the technical field of medicine and discloses the application of diphenylheptane compounds in the preparation of medicines or food for preventing and treating psoriasis. The diphenylheptane compound has the structure shown in formula I: Wherein, the 4-5 position of the diphenylheptane compound is a single bond or a double bond, and the 6-7 position is a single bond or a double bond; R ₁ is double bond oxygen or acetoxy; R ₂ is hydrogen, hydroxy or methoxy. The diphenylheptane compound of the invention has inhibitory effect on the proliferation of human skin T cell lymphoma cell line HH cells and human immortalized epidermal keratinogenic cell line HaCaT cells, and can be applied to the preparation of medicines or food for preventing and treating psoriasis.

The invention relates to an exfoliating skin cream which is composed of a wormwood extract, a licorice essence, an oat essence, pseudo-ginseng, glycerol stearate, glycerin, deionized water, white oil, and vitamin A. According to the exfoliating skin cream, the wormwood extract is adopted as a main raw material. The exfoliating skin cream has the functions of epidermal keratinocyte softening, dead skin removing, pore unblocking, rough and dull skin ameliorating, skin nourishing, skin tone improving, and the like. The main components wormwood, licorice, and oat are skin-protecting and skin-nourishing herbal plants recorded in Compendium of Materia Medica. The exfoliating skin cream is a traditional Chinese medicine conditioning product causing no side effect. The exfoliating skin cream is suitable for various skins.