106 results about "Position effect" patented technology

A surgical forceps includes a housing having a movable handle coupled thereto and movable between an initial position and a compressed position. A drive bar is operably coupled to the movable handle such that movement of the movable handle between the initial and compressed positions effects longitudinal translation of the drive bar. The drive bar further includes a coupling member disposed at a distal end thereof. An end effector assembly includes first and second jaw members movable between spaced-apart and approximated positions. Each jaw member defines a cam slot. A cam pin is slidably disposed within the cam slot of each of the jaw members. The coupling member is configured to releasably engage the cam pin such that movement of the movable handle between the initial position and the compressed position effects movement of the jaw members between the spaced-apart position and the approximated position.

Introducing blocks of foreign genes in a single operon would avoid complications such as position effect and gene silencing inherent in putting one gene at a time into random locations in the nuclear genome. Cloning several genes into a single T-DNA does not avoid the compounded variable expression problem encountered in nuclear transgenic plants. This disclosure shows that a bacterial operon can be expressed in a single integration event as opposed to multiple events requiring several years to accomplish. Expression of multiple genes via a single transformation event opens the possibility of expressing foreign pathways or pharmaceutical proteins involving multiple genes. Expressing the Cry2aA2 operon, including a putative chaperonin to aid in protein folding, in the chloroplast via a single transformation event leads to production of crystalized insecticidal proteins. Expressing the Mer operon via a single transformation event leads to a phytoremediation system.

The invention discloses a method for targetedly integrating an exogenous gene into a target gene of a mammal, which is implemented by targetedly integrating an exogenous gene into a target gene by utilizing a CRISPR/Cas9 system, and especially targetedly integrating a Cre gene to a first exon of a mouse Enpp1 gene. The technique has the advantages of high integration efficiency (20%) and simple operation step, only needs to construct one exogenous gene vector, and can not be influenced by the position effect.

The invention discloses a positioning method and a device based on calibration point, which belongs to the field of wireless networks. The positioning method comprises the following steps: coordinate information of at least four calibration points in a preset area to be measured is determined; a mirror point of each base station is determined according to the position relationship between at least three base stations in the area to be measured and the calibration points, and the coordinate of the mirror point of each base station is set; a relational expression is built according to the position relationship between the coordinate information of at least four calibration points, the coordinates of mirror points of at least three base stations as well as the base stations in the area to be measured and the calibration points, and the coordinate information of a positioning terminal is obtained through operating and solving the relational expression. The invention utilizes the coordinate of a presorted calibration point to inversely calculate the coordinate of the mirror point formed by the last reflection of a signal arriving at the calibration point through the characteristic in the process that the signal sent by the base stations is transmitted to the calibration point, further works out the coordinate information of the positioning terminal, eliminates the influence of a non-line-of-sight error on positioning effect, and improves the precision of the positioning.

For a radiographic image detector which includes an imaging plane including two-dimensional matrix of pixel sections, each pixel section storing, when exposed to radiation, a charge according to amount of the radiation, and which is used to be exposed to radiation transmitted through the same subject each time the detector is shifted along a predetermined axis of shift, an inclination of the two-dimensional matrix relative to the axis of shift of the radiographic image detector is detected. The inclination is detected by applying radiation two times to the detector at different radiation application positions effected by the shift of the detector so that a common marker is imaged during each radiation application; carrying out a reading operation after each radiation application to acquire image data representing image information of the marker; and detecting the inclination based on a positional relationship between marker images represented by the image data.

The invention discloses a GPS (Global Position System) position method and device. The speed and the accuracy rate of GPS position are increased by optimizing the communication speed between a mobile phone and a relevant GPS server. Particularly, network packet loss of multiple servers is tested; the server with an optimal test result is selected as the server for the current position operation, so that the situation of a poor position effect caused by the inappropriate server is avoided; and the method and the device are automatic without manual setting of a user.

A nailer includes a gun body, a magazine, a feed plate, a washer barrel, a catch plate, and a locking ring. Thus, the washer is closely positioned between the catch flange of the feed plate and the locking ring, thereby facilitating a user combining the washer with the nail. In addition, the locking ring is tensioned only when the washer passes through the locking ring, so that the locking ring is not maintained at the tightened state constantly, thereby preventing the locking ring from producing fatigue, and thereby enhancing the lifetime of the locking ring. Further, the locking ring is used to stop washers made of plastic or metallic material, thereby enhancing the versatility of the nailer.

The invention discloses an intelligent integrated circuit. The intelligent integrated circuit comprises the design layout of a receiving integrated circuit (IC). The design layout comprises multiple non-overlapping IC areas, and the IC areas are divided into multiple groups based on the position effect analysis of the IC design layout to enable all the IC areas in each group to have basically identical position effects; the IC design layout is corrected by means of a correcting model comprising the position effects, so that a corrected IC design layout is generated. By the adoption of the scheme, corrected IC areas are copied to other IC areas in a corresponding group, the corrected IC design layout is stored in a tangible computer readable medium to be used for further IC process, and market application value is high.

The invention provides a Ti plasmid aspergillus niger gene replacement expression vector and application thereof, and belongs to the technical field of molecular biology. The T-DNA (Triple helix Deoxyribose Nucleic Acid) region elements of the Ti plasmid aspergillus niger gene replacement expression vector are arranged in the following sequence: an aspergillus niger target gene promoter, a multiple cloning site, an aspergillus niger target gene terminator, an aspergillus nidulans 3-phosphoglyceraldehyde dehydrogenase gene promoter PgpdA, an aspergillus niger selection marker gene and an aspergillus niger target gene terminator. According to the invention, a target gene is integrated at the site of the aspergillus niger target gene through homologous recombination, and the target gene is regulated and controlled by a target gene promotor of high expression; therefore, the position effect of transgenosis is eliminated and the expression level is improved.

The invention discloses an industrial forging mechanical clamp hand. The industrial forging mechanical clamp hand comprises a machine base and a control cabinet. The machine base is provided with a rotation mechanism. The rotation mechanism comprises a rotation motor and two parallel rotation discs. Two stand columns are arranged above the rotation discs and fixedly arranged on the upper surfaces of upper rotation disc bodies of the rotation discs through bolts. A fixing plate is arranged above the stand columns. A lifting air cylinder is arranged on the fixing plate and fixed to the upper surface of the fixing plate through bolts. A hydraulic cylinder is arranged between the stand columns. Sliding grooves are formed in the parts, making contact with the inner walls of the stand columns, of the hydraulic cylinder. According to the industrial forging mechanical clamp hand, the three-point positioning effect on a workpiece is achieved through three sets of clamp arms, the forging difficulty is lowered, the manual forging manner is omitted, the forging error is avoided, the using effect is good, and the service life is long; and the clamping force is large, the structure is compact, and the clamp arms are provided with clamp teeth, so that the friction force is increased, and objects are not prone to falling off.

The invention provides a recombined saccharomyces cerevisiae strain for producing ethyl alcohol, a construction method of the recombined saccharomyces cerevisiae strain and a method for producing ethyl alcohol through the recombined saccharomyces cerevisiae strain. According to the recombined saccharomyces cerevisiae strain for producing ethyl alcohol, a wild type saccharomyces cerevisiae strain is used as an original strain, an XI xylose metabolism pathway is introduced into the original strain, the expression of four key genes in a PPP pathway is enhanced, meanwhile, the aldose reductase gene GRE3 is knocked out, the nitrobenzene phosphatase gene PHO13 is optionally knocked out, and XI protein and XK protein introduced exogenously are positioned to a yeast cell membrane through the membrane positioning effect of a membrane positioning group. The recombined saccharomyces cerevisiae strain for producing ethyl alcohol can efficiently carry out xylose metabolism, so that co-fermentation of C5 sugar and C6 sugar is achieved, and therefore the higher ethyl alcohol conversion rate is achieved.

The invention belongs to the field of molecule biological medicine, and relates to a magnetic polyacrylic acid-modified nanometer pipe and a method for preparing the same. A multi-wall carbon nanometer pipe is modified by polyacrylonitrile and hydrolyzed under acid condition to obtain a polyacrylic acid-modified water-soluble nanometer pipe containing large quantity of carboxyl groups; by the water-phase chemical co-precipitation method, a magnetic ferroferric oxide-polyacrylic acid-modified multi-wall carbon nanometer pipe composite with the diameter of between 10 and 80nm is prepared, and the composite has good lymphotaxis and strong magnetic response; tests show that a medicine carrier can effectively adsorb chemical treatment medicines to a lymphatic system, slowly release the chemical treatment medicines in a metastasis lymph node of a malignant tumor, has good magnetic field positioning effect and obviously inhibits the proliferation and metastasis of the tumor cell of the lymph node. The invention provides a novel intervention means for lymphatic metastasis of the malignant tumor.

In the present invention, to make corrective matching thereof, it is designed as follows; position effect of defects coordinates, which are output from an inspection apparatus, is allowed, coordinates of inspected data are mutually corrected, and a state of coincidence or non-coincidence among a plurality sets of inspected data is output or displayed. Inspection data is designed to include kinds, kinds difference and dimension of defects. A state of coincidence or non-coincidence between inspected data is designed to be output or displayed appropriately, by kinds or dimensions, or by a grouping thereof, of a defects object. The same sample is inspected by every time of passing a production step, and a state of data increase or decrease, or coincidence or non-coincidence between the inspected data is designed to be output or displayed.

The invention discloses a mesh network node positioning method which comprises (1) predicting a trajectory according to a historical trajectory; (2) controlling a transmitting power of a reference node to enable that a power value of a mobile node is the largest; and (3) performing dormancy processing on an unrelated reference node to achieve node energy control. According to the disadvantages of the existing Zigbee node positioning effect of CC2431 that the energy consumption is high, the precision is low; when arranging wireless sensor network reference nodes on a large scale, the overall energy consumption of the system is increased, the energy intensity of signals is enhanced, and the positioning effect of the system is essentially improved, but the positioning accuracy is not effectively improved; the mesh network node positioning method is aimed at providing a Zigbee positioning algorithm which is high in precision, saves the energy and reduces the consumption.

A substrate loader adapted to load and unload a substrate to and from a moveable holder having a seal. The substrate loader has a base and a holder support frame coupled to the base, the holder support frame adapted to repeatably position the moveable holder relative to a predetermined datum. The substrate transport is coupled to the base and having a substrate chuck and adapted to move and transport the substrate relative to the holder. The substrate transport is deterministically positioned relative to the predetermined datum and is adapted to move the substrate from a first position, with the substrate captured by the moveable holder, to a second position with the substrate disengaged from the holder and the seal, the substrate transport movement of the substrate from the first to the second position effecting disengagement from the holder and the seal substantially without contacting the substrate.

The present invention discloses transgenic monocot plants in which the plastid genome has been genetically engineered. The bioconfined genetically engineered monocot crops have transgene-free pollen grains which eliminate or dramatically reduce transgene flow. The present invention discloses plastid transgenesis technology having the additional advantages of the absence of gene silencing and position effect variation, the ability to express polycistronic messages from a single promoter, integration via a homologous recombination process that facilitates targeted gene replacement and precise transgene control, and sequestration of foreign proteins in the organelle which prevents adverse interaction with the cytoplasmic environment.

The invention provides a method for efficiently producing recombinant proteins in mammary glands by utilizing artificial chromosomes (including BAC, PAC and YAC). In the method, a recombinant technology is adopted to completely replace target genes on artificial chromosomes of mammary gland-specific expressed proteins (including as1-casein, beta-casein, beta lactoglobulin, rabbit or mouse whey acidic protein and the like) of a mammal with an exogenous gene, so that the exogenous gene obtains an intact regulatory sequence of a mammary gland high-level expression target gene, the 'position effect' is avoided, the higher-level expression of human lysozyme or other recombinant proteins in the mammary glands can be realized, the success rate of transgenic breeding is improved, and the cost forproducing and purifying the recombinant proteins at a later stage can be saved at the same time.

The invention discloses classification stacking equipment based on a manipulator. The classification stacking equipment comprises a carrier conveying line; the carrier conveying line includes a feeding roller way conveying belt, a driven roller way and a positioning roller way conveying belt arranged in sequence; a film tearing identification mechanism is arranged above the carrier conveying line; a base is arranged on one side of the positioning roller way conveying belt; a six-shaft manipulator is arranged on the base; a grabbing mechanism is arranged on the six-shaft manipulator; and a good product placing area, a defective product placing area and a labeling mechanism are arranged at the peripheral edge of the six-shaft manipulator. The classification stacking equipment is excellent in positioning effect and high in precision, reduces manual intervention, and is low in product harm.

The invention discloses a contrast-based weak supervision time sequence action positioning evaluation method and system, and belongs to the field of computer vision and mode recognition. The contrast-based weak supervision time sequence action positioning evaluation method comprises the steps: carrying out the modeling on the contrast information in a video; then utilizing the contrast to obtain afragment-level edge degree measurement; and finally, scoring any candidate video segment by using the obtained edge degree and a known segment level action classification result. According to the contrast-based weak supervision time sequence action positioning evaluation method, three aspects of starting, ending and content are comprehensively considered, and finally, an evaluation result with relatively high reliability is given; and by means of the evaluation result, the time sequence action positioning accuracy can be greatly improved. According to the contrast-based weak supervision timesequence action positioning evaluation method, the action positioning effect of any video time period can be comprehensively evaluated under the weak supervision condition, and only video-level category labeling is needed, and data labeling does not depend on complex action boundaries, and the labeling burden of the data can be greatly reduced.

The invention discloses a spindle blade of a textile machine spindle. The spindle blade of the textile machine spindle comprises a hollow rod body (10). The rod body (10) is provided with four openings (20) and telescopic blocks which can be stretched out and drawn back in the openings (20). The spindle blade of the textile machine spindle further comprises a lever, the telescopic blocks are connected at the two ends of the lever in a movable mode, the lever (41) is arranged on a cross lever (51), the cross lever penetrates through the middle point of the lever, a cylindrical spring (60) is arranged between the cross lever and the lever, and the cross lever penetrates through the axial line of the rod body (10). When a bobbin is arranged on the spindle blade in a sleeved mode from top to bottom, the lower end of the bobbin makes contact with the inclined faces of the telescopic blocks, the telescopic blocks retract towards the inner side of the rod body, the telescopic blocks drive the lever to rotate around the cross lever, the lever drives the other end of each telescopic block to retract towards the inner side of the rod body. Due to the fact that the cross lever penetrates through the middle point of the lever, the cross lever penetrates through the axial line of the rod body, and the retracting distances towards the inner side of the rod body of the two telescopic blocks are the same. By means of the technical scheme, the center positioning effect of the bobbin is achieved.

The invention relates to the technical field of tree vegetative propagation and discloses a breeding method of excellent fir wild resources. The breeding method is characterized in that a fir plant semi-lignified lateral branch serves as an explant to perform tissue culture to obtain a tissue culture seedling, and after the tissue culture seedling is subjected to acclimatization and transplanting, a press-drying sprouting promotion method is adopted to break through a position effect of a fir adult tree lateral branch, so that a high-quality juvenile vegetative propagation material is obtained; and the obtained vegetative propagation material is subjected to tissue culture to obtain fir multiple shoots and rooting seedlings, which can be subjected to micropropagation in quantity, so that efficient in-vitro conservation of the excellent fir wild gene resources is realized, and high-quality fir tissue culture trees can be quickly bred. A production mode of mutual butting and mutual promotion of 'tissue culture seeding-cutting orchard-cutting propagation' is established, and effective resource conservation and large-scale production of vegetative trees are realized.

The invention relates to the technical field of electronic products, and discloses a sound containing device which is convenient for adjusting the sounding direction, including a base, the left and right sides of the base are fixedly connected with fixing plates, and the top of the fixing plate is threadedly connected with a mounting Bolts, the bottom of the base is provided with two left-right symmetrical mounting grooves, and the inner surface of the top of the mounting grooves is fixedly connected with a limit spring. The audio containing device which is convenient to adjust the sounding direction can achieve the rolling wheel to be put away and put down by setting the installation groove, the limit spring, the first U-shaped plate, the roller and the installation bolt to loosen and tighten the installation bolt, and then At the same time, it has the effect of easy movement and stable installation, which greatly facilitates the movement and installation of the staff, and then achieves the effect of improving the staff's daily movement of the sound position, and achieves the effect of being put into use quickly by being easy to move and stable, so as to achieve Improve the efficiency of the user's arrangement of indoor audio.