Breeding method of excellent fir wild resources

A field, fir technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of high cost, shortened breeding and utilization cycle, low efficiency, etc., and achieve the effect of rapid breeding and effective preservation

Active Publication Date: 2017-09-26
GUANGDONG ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide an effective breeding method of excellent gene resources of Chinese fir in the wild, which can significantly shorten the breeding and utilization cycle, greatly improve the preservation time and efficiency of excellent genetic resources of Chinese

Method used

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  • Breeding method of excellent fir wild resources
  • Breeding method of excellent fir wild resources

Examples

Experimental program
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Effect test

Embodiment 1

[0067] (1) On October 15, 2013, the middle and upper side branches of No. 7 clone were collected from an 8-year-old Chinese fir clone determination forest located in Shaoguan, Guangdong Province, and the branches were properly trimmed, and the top and tip parts were retained. The length of the branches was 10-15 cm. Wrap it with a clean wet towel, put it in a plastic sealed bag, and then put it into a foam box with ice cubes. It is required that the ice cubes cannot directly contact the sprouts. The temperature in the box is controlled at 4°C to 10°C. The material was brought back to the laboratory for timely processing.

[0068] (2) In the laboratory, remove all the leaves from the branches collected from the field, put them into a clean measuring cup with a volume of 2L, add half a spoonful of neutral washing powder and 2 drops of Tween-80, soak and shake for 3min, and then place Rinse under running water for 25 minutes, take out the materials, cut off the lignified parts, k...

Embodiment 2

[0084] (1) On October 10, 2013, the middle and upper side branches of the TL5 clone were collected from the 7-year-old Chinese fir clone determination forest located in Yunfu City, Guangdong Province, and the branches were properly trimmed, and the top part was kept, and the length of the branches was 10-15cm. Wrap it with a clean wet towel, put it in a plastic sealed bag, and then put it into a foam box with ice cubes. It is required that the ice cubes cannot directly contact the sprouts. The temperature in the box is controlled at 4°C to 10°C. The material was brought back to the laboratory for timely processing.

[0085] (2) In the laboratory, remove all the leaves from the branches collected from the field, put them into a clean measuring cup with a volume of 2L, add half a spoonful of neutral washing powder and 2 drops of Tween-80, soak and shake for 5min, and then place Rinse under running water for 25 minutes, take out the materials, cut off the lignified parts, keep th...

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Abstract

The invention relates to the technical field of tree vegetative propagation and discloses a breeding method of excellent fir wild resources. The breeding method is characterized in that a fir plant semi-lignified lateral branch serves as an explant to perform tissue culture to obtain a tissue culture seedling, and after the tissue culture seedling is subjected to acclimatization and transplanting, a press-drying sprouting promotion method is adopted to break through a position effect of a fir adult tree lateral branch, so that a high-quality juvenile vegetative propagation material is obtained; and the obtained vegetative propagation material is subjected to tissue culture to obtain fir multiple shoots and rooting seedlings, which can be subjected to micropropagation in quantity, so that efficient in-vitro conservation of the excellent fir wild gene resources is realized, and high-quality fir tissue culture trees can be quickly bred. A production mode of mutual butting and mutual promotion of 'tissue culture seeding-cutting orchard-cutting propagation' is established, and effective resource conservation and large-scale production of vegetative trees are realized.

Description

technical field [0001] The invention relates to the technical field of asexual propagation of forest trees, in particular to a method for breeding excellent wild Chinese fir resources. Background technique [0002] Chinese fir (Cunninghamia Lanceolata) has the characteristics of fast growth, good material quality and high yield rate, and is an important commercial timber and afforestation tree species in southern my country. At present, my country's fir genetic improvement work has entered the fourth-generation improvement stage, and a large number of genetic resources with excellent characteristics have been selected and bred, with remarkable results. Due to the growth characteristics of forest trees, the complexity of natural conditions of forest land, and the pressure of plantation forest felling and utilization, how to preserve excellent genetic resources in a timely and effective manner, and how to transform scientific research results in a timely manner are hot issues ...

Claims

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Application Information

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IPC IPC(8): A01G17/00A01H4/00A01G9/10
CPCA01G17/005A01H4/008
Inventor 韦如萍胡德活晏姝郑会全王润辉
Owner GUANGDONG ACAD OF FORESTRY
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