19 results about "Multigene expression" patented technology

The invention discloses a polygene expression cassette and a method for expressing I-type collagen in soybeans, and the method realizes rapid and efficient production of recombinant I-type collagen through a soybean seed expression system containing an optimized polygene expression cassette to express I-type collagen massively and stably. Moreover, the soybeans are the first oil crop in the world, the seeds of the soybeans are natural and free of toxic secondary metabolites, protein extraction does not need a complex detoxification process, in addition, the soybean seeds are stable in normal-temperature storage, the problem of rapid degradation of a leaf expression system (such as tobacco) is avoided, and the shelf life of the raw materials is remarkably prolonged.

The invention discloses a CAR-T cell modified by an interleukin 15 receptor alpha mutant and application of the CAR-T cell, and particularly relates to a gene engineering medicine. The CAR-T cell is integrated with a multi-gene expression unit through an SB100X transposon system, wherein the multi-gene expression unit comprises opti-mIL-15 (T62G / L55F), opti-mIL-15R alpha-Sushi (Q199E / D203A), a GPI anchoring sequence, a (G4S) 3 flexible connecting peptide, a targeted CAR gene and an iC9 safety switch, and a membrane binding type IL-15 / IL-15R alpha compound supported by an autocrine signal is formed. The affinity and stability of IL-15 signals are enhanced through mutants, efficient integration and co-expression are achieved through non-viral vectors, the in-vivo durability, amplification capacity and anti-tumor activity of CAR-T cells are remarkably improved, and meanwhile a cell factor local delivery and induction type safety switch system is combined through a membrane.

The invention provides a method for regulating and controlling polygene expression through single input signal frequency illumination amplitude and duty ratio. The method comprises the following steps: S1) constructing an engineering microorganism strain, wherein the engineering microorganism strain comprises a signal response module, a transcription activation module and a multi-target gene parallel expression module; s2) performing signal regulation and control by utilizing different frequencies, duty ratios and illumination amplitudes of the photostimulation signals to realize expression regulation and control of the target gene; and S3) performing quantitative detection on the expression product of the target gene. Compared with the prior art, the method has the advantages that a plurality of downstream genes can be controlled at the same time through single input by imitating the second messenger principle, that is, quantitative expression of a plurality of products can be realized by utilizing a single structure, and quantitative data of different target genes are more comparable while the operation is simple.

The invention discloses a neuropeptide S for promoting mouse spinal cord injury repair by regulating and controlling endogenous NSPCs to differentiate into neurons and perform axonal regeneration, and belongs to the field of biological medicine and nerve repair. According to the technical scheme of the invention, a complete T11 spinal cord injury mouse model is transplanted and treated by adopting a thermo-sensitive hydrogel stent loaded with NPS. In-vitro experiments show that NPS can significantly promote neural stem / progenitor cells to differentiate into neurons and prolong axons. In-vivo experiments show that the immune response of an injured area is relieved, endogenous nerve stem / progenitor cells migrate and are differentiated into functional neurons, axons extend, more synapses are formed, and the movement function of hind limbs is obviously recovered. NPS activates downstream Ca < 2 + > and cAMP signal channels through an NPS-NPSR axis, and regulates and controls polygene expression, so that nerve regeneration and functional recovery are promoted. The NPS provided by the invention provides an efficient and feasible treatment strategy for spinal cord injury repair, and has an important clinical application prospect.

This invention discloses the application of a biomarker in assessing the risk of prostate cancer recurrence, belonging to the field of tumor molecular diagnostics and bioinformatics technology. The biomarker includes at least one of the TACR2 and LPIN3 genes, and RCCD1, PRSS27, and NAP1L5, with the core detection focusing on the methylation-transcriptional combined characteristics of the TACR2 gene. This invention obtains patient samples, uses RT-qPCR to detect the expression levels of multiple genes, and methylation-specific PCR to detect the methylation level of the TACR2 promoter region. A risk scoring model is constructed using LASSO regression, and patient prognosis is stratified based on the score. This invention solves the problems of traditional assessment indicators being affected by tumor heterogeneity and having limited predictive accuracy. It is the first to use TACR2-related multi-omics characteristics for prostate cancer recurrence assessment. The multi-gene combined model improves predictive accuracy and can be developed into a standardized testing kit, providing a scientific basis for clinical recurrence risk assessment and personalized treatment, with significant industrial and clinical application value.

The invention discloses an engineering strain for improving the yield of cordycepin of cordyceps militaris based on polygene combination expression and precursor engineering and a construction method of the engineering strain, a strong promoter gpdA is adopted for driving, a P2A peptide connection strategy is utilized, and two polygene expression modules are constructed: firstly, cordycepin biosynthesis key genes cns1 and cns2 are overexpressed at the same time, and then the cordycepin biosynthesis key genes cns1 and cns2 are overexpressed at the same time; the terminal catalytic efficiency of the synthetic route is enhanced; and 2, the cns1 and a precursor supply gene nt5e are subjected to combined expression, so that the synergistic enhancement of precursor supply and terminal catalysis is realized. And introducing the expression module into cordyceps militaris through an agrobacterium-mediated method to obtain an engineering strain with genetic stability. A fermentation experiment shows that the cordycepin yield of the cns1-P2A-cns2 engineering strain reaches 186.5 mg / L, which is 2.3 times that of a wild type; and the yield of the cns1-P2A-nt5e engineering strain is further increased to 252.6 mg / L, which is 3.1 times that of a wild type. According to the invention, the induction cost is avoided, the construction is simple, the stability is strong, and a new strategy is provided for industrial efficient production of cordycepin.

According to the invention, a stable multi-gene expression system is constructed in chlamydomonas chloroplast for the first time, the expression system comprises a plurality of promoters and terminators, five gene expression units are successfully integrated at a single site of a chlamydomonas chloroplast genome, and meanwhile, one gene expression unit is integrated in a chlamydomonas nuclear genome. The establishment of the system promotes the development of chloroplast synthetic biology and metabolic engineering, and provides key technical support for synthesizing high-value compounds. On the basis of the gene expression system, a chlamydomonas engineering strain for expressing carboxylase is also constructed, a key foundation is laid for converting carboxylase into C3 crop chloroplast to improve the photosynthesis efficiency, meanwhile, technical support is provided for constructing a functional CO2 concentration mechanism in higher plants, and the final target is to improve the photosynthesis efficiency. Therefore, the crop yield is improved.

This invention relates to a promoter derived from *Chlorella sorokinense*, the sequence of which is shown in SEQ ID NO:1; a signal peptide derived from *Chlorella sorokinense*, the protein sequence of which is shown in SEQ ID NO:2; and a method for expressing exogenous genes in *Chlorella sorokinense*. This invention identifies for the first time an endogenous promoter and a chloroplast-targeting signal peptide from *Chlorella sorokinense*, and constructs a stable expression system in *Chlorella sorokinense*. Using β-carotene ketolase as an example, exogenous gene expression units were successfully integrated into the chloroplast genome and nuclear genome of *Chlorella sorokinense*, and their effective expression was achieved. Based on this research, we can further introduce multi-gene expression into *Chlorella sorokinense*, fully releasing its industrialization potential, promoting the development of chloroplast synthetic biology and metabolic engineering, and propelling *Chlorella sorokinense* towards becoming a highly efficient and controllable cell factory.

The present invention provides transgenic animals (e.g., transgenic porcine animals), organs, tissues, and cells derived from the transgenic animals that are particularly useful for xenotransplantation therapies. In particular, the present invention provides transgenic porcine animals, as well as organs, tissues and cells derived from the transgenic porcine animals, which lack any expression of a functional alpha 1,3 galactosyltransferase (GTKO) gene and comprise at least six transgenes under the control of at least three promoters within a single multi-gene expression vector, and further comprise at least four additional genetic modifications. Also provided are methods of making the transgenic animals (e.g., transgenic porcine animals), and methods of using the transgenic animals, organs, tissues, and cells derived from the transgenic animals for xenotransplantation therapies and treating a disease or condition.

This application relates to the field of molecular subtyping technology, specifically disclosing a molecular subtyping method for NK / T-cell lymphoma based on multi-gene expression characteristics and its application. The molecular subtyping method provided in this application can systematically reflect the molecular heterogeneity of NK / T-cell lymphoma, offers a standardized and reproducible molecular subtyping detection protocol, and can also assist in assessing patient prognostic risks and provide a reference for treatment decisions. The molecular subtyping method provided in this application is applicable to various conventional molecular detection platforms and has good clinical application value.

The invention discloses a method for constructing a multi-gene expression vector or a multi-target gene editing vector. The multi-gene expression vector or the multi-target gene editing vector comprises a transformation vector (pDF11) and two donor vectors (p103A and p103B), the transformation vector carries a CRI SPR-Act3.0 transcriptional activation system, a plant screening gene (hpt I I), two I-SceI restriction enzyme cutting sites in opposite directions, and a kanamycin (Km) resistance vector; the two donor vectors comprise a complete gRNA expression cassette and respective antibiotic selection marker genes, the two donor vectors respectively have spectinomycin (Spe) and ampicillin (Amp) resistance, and the two sides of the antibiotic selection marker genes also contain two I-Sce I restriction enzyme cutting sites in opposite directions; compared with the prior art, the invention has the advantages that the construction of the multi-gene and multi-target transcription activation vector is realized, the vector system constructs a series of single-target and multi-target transcription activation vectors, and one vector simultaneously contains four genes and 10 targets of two PtoWRKY41 targets, two PtoWRKY31 targets, two PtoWRKY70 targets and four PtoWRKY41a targets.

The invention relates to the technical field of biology, in particular to a novel recombinant vaccinia virus shuttle vector and application thereof.The shuttle vector comprises homologous recombination arms composed of an upstream gene sequence and a downstream gene sequence of vaccinia virus thymidine kinase TK, and the homologous recombination arms are VACV-093 and VACV-095 respectively; the kit further comprises three exogenous gene expression cassettes, and the three exogenous gene expression cassettes respectively contain a pTK11 heterozygous promoter, a pSE / L promoter and a p7.5 K promoter. The invention innovatively provides a new strategy for completely removing a TK open reading frame, retaining a pTK promoter and constructing a hybrid strong promoter, and solves the problem of promoter limitation during multi-gene expression and the problem of low screening efficiency of recombinant vaccinia virus.

The invention relates to a method of predicting an outcome of a breast cancer subject, comprising determining or receiving the result of a determination of a gene expression profile comprising the expression levels of four or more genes selected from a first, a second and / or a third gene expression profile, wherein the first gene expression profile comprising one or more immune defense response genes selected from the group consisting of: AIM2, APOBEC3A, CIAO1, DDX58, DHX9, IFI16, IFIH1, IFIT1, IFIT3, LRRFIP1, MYD88, OAS1, TLR8, and ZBP1, and / or the second gene expression profile comprising one or more T-Cell receptor signalling genes selected from the group consisting of: CD2, CD247, CD28, CD3E, CD3G, CD4, CSK, EZR, FYN, LAT, LCK, PAG1, PDE4D, PRKACA, PRKACB, PTPRC, and ZAP70, and / or the third gene expression profile comprising one or more PDE4D7 correlated genes selected from the group consisting of: ABCC5, CUX2, KIAA1549, PDE4D, RAP1GAP2, SLC39A11, TDRD1, and VWA2, said gene expression profile being determined in a biological sample obtained from the subject, determining the prediction of the outcome based on the gene expression profile comprising the expression levels of the four or more genes, wherein said prediction is a favourable risk or a non-favourable risk of breast-cancer related death, loco-regional recurrence and / or distant recurrence.

The invention discloses a tumor target spot and drug quantification system and method based on a multi-gene expression profile, and the method comprises the steps: collecting tumor reference data, and building a database; determining the transcriptome of the tissue sample to obtain the original expression counts of the patient, and carrying out standardization treatment; performing target quantification and recognition on each gene, and calculating a comprehensive score corresponding to each target through a target comprehensive quantification function; identifying gene fusion information of each target spot by using the transcriptome of the tissue sample, and identifying gene mutation information of each target spot by using the high-depth transcriptome of the tissue sample; according to the method, the potential curative effect of each drug is subjected to comprehensive quantitative analysis, the comprehensive drug effect value of the drug is calculated through the drug comprehensive quantitative function and is sequenced, and personalized drug targets of 16 types of tumors can be comprehensively evaluated, so that a scientific basis can be provided for clinical prognosis evaluation, treatment schemes and drug selection of tumors; and a more accurate treatment scheme is formulated, so that the pertinence of treatment is improved.

The application discloses a tumor and paired cell line whole transcriptome RNA standard substance and a preparation method and application, wherein the tumor and paired cell line is a lung cancer and paired paracancer tissue immortalized cell line, is preserved in the China General Microbiological Culture Collection Center, and the preservation date is October 11, 2025; the lung cancer cell line biological preservation number is CGMCC No. 46703; and the paired paracancer tissue cell line biological preservation number is CGMCC No. 46704. The RNA standard substance includes two samples, is whole transcriptome RNA extracts of the tumor and paracancer tissue immortalized cell line of the same individual source in the Chinese genetic background, and the quantity value is the copy number concentration and the ratio of high confidence genes in the two samples. The whole transcriptome RNA standard substance can be used for performance evaluation of absolute quantification or relative quantification of multi-gene expression of detection data of different transcriptome sequencing technologies and sequencing platforms.

The present invention provides transgenic animals (e.g., transgenic porcine animals), organs, tissues, and cells derived from the transgenic animals that are particularly useful for xenotransplantation therapies. In particular, the present invention provides transgenic porcine animals, as well as organs, tissues and cells derived from the transgenic porcine animals, which lack any expression of a functional alpha 1,3 galactosyltransferase (GTKO) gene and comprise at least six transgenes under the control of at least three promoters within a single multi-gene expression vector, and further comprise at least four additional genetic modifications. Also provided are methods of making the transgenic animals (e.g., transgenic porcine animals), and methods of using the transgenic animals, organs, tissues, and cells derived from the transgenic animals for xenotransplantation therapies and treating a disease or condition.

The invention discloses a tumor and paired cell line whole transcriptome RNA standard substance and a preparation method and application thereof.The tumor and paired cell line is a lung cancer and paired para-carcinoma tissue immortalized cell line and is preserved in the China General Microbiological Culture Collection Center (CGMCC) on October 11, 2025, and the preservation number is CGMCC NO. The biological preservation number of the lung cancer cell line is CGMCC (China General Microbiological Culture Collection Center) No.46703, and the biological preservation number of the para-carcinoma tissue cell line is CGMCC No.46704. The RNA standard substance comprises two samples which are whole transcriptome RNA extracts of tumor and para-carcinoma tissue immortalized cell lines with the genetic background of Chinese and from the same individual, and the quantity value is the copy number concentration and the ratio of high-confidence genes in the two samples. The whole transcriptome RNA standard substance can be used for performance evaluation of absolute quantification or relative quantification of multi-gene expression of different transcriptome sequencing technologies and sequencing platform detection data.

The invention discloses an application of a COTL1high NK cell as a biomarker in prognosis of liver cancer. Based on in-depth study on tumor microenvironment, the invention finds that COTL1high NK cells are highly enriched in liver cancer which responds to immune checkpoint blocking (ICB) treatment and are related to good prognosis. According to the method, characteristic genes of COTL1high NK are analyzed, dynamic and polygene expression information is obtained, a marker capable of predicting liver cancer prognosis is found, a liver cancer prognosis model is established according to the marker and is used for predicting prognosis of a liver cancer patient, dynamic and polygene tumor microenvironment information can be obtained through the prediction method, and the prognosis of the liver cancer patient can be predicted according to the dynamic and polygene tumor microenvironment information. The prognosis of the liver cancer patient is accurately predicted.

The invention discloses a method for predicting OPU-IVF blastocyst development and embryo production performance by identifying a multi-gene expression pattern in an oocyte and a cumulus cell, which comprises the following steps: detecting expression patterns of mitochondrial functional genes ISCU and IGF2BP3 in the oocyte and an oocyte-cumulus cell interaction gene CTNND1; the method further comprises the step of detecting expression modes of an oxidative phosphorylation and metabolism regulation pathway SOX4, a gene KLF12 participating in cell cycle control and apoptosis regulation, an oxidative phosphorylation and metabolism regulation pathway OGT and an oocyte marker FST in cumulus cells. The expression modes of the genes can predict the embryonic development potential of oocytes after in-vitro maturation and in-vitro fertilization, important genetic information prediction is provided for predicting the reproductive performance of livestock, especially applying the in-vitro fertilization technology to embryo production of large livestock, and the breeding and breeding process of high-value livestock populations is promoted.