4 results about "Gene assembly" patented technology

The invention discloses a high-fidelity DNA polymerase as well as a preparation method and application thereof, and relates to the technical field of biology. The invention provides a high-fidelity DNA polymerase, the high-fidelity DNA polymerase is formed by fusing a DNA binding protein and a Pfu DNA polymerase, the amino acid sequence of the DNA binding protein is as shown in SEQ ID NO: 3, the amino acid sequence of the Pfu DNA polymerase is as shown in SEQ ID NO: 5, and the amino acid sequence of the high-fidelity DNA polymerase is as shown in SEQ ID NO: 10. The high-fidelity DNA polymerase disclosed by the invention can splice and assemble oligonucleotides into long-fragment DNA, can reduce the number of mismatched bases, has a stable effect in splicing and assembling of multiple long-fragment DNA with different sequences, and has an important practical value for a genome assembling technology.

The invention relates to methods and compositions useful for routing and tracking multiple mobile units within a microfluidic device. Mobile units may be routed through a plurality of chemical environments, and the mobile units may be tracked to determine the path and / or environments that the mobile units have routed through. Mobile units may be routed in accordance with a predetermined algorithm. Mobile units may be routed through microfluidic devices in ordered flow. Mobile units routed through the microfluidic device can be used to perform various chemical reactions uniquely associated to the units, including without limitation peptide synthesis, enzymatic gene synthesis and gene assembly.

The present invention aims to provide a method for constructing metabolically modified microbial strains that produce useful compounds in high quantities, and to provide metabolically modified microbial strains that produce useful compounds in high quantities. [Solution] The present invention relates to a method for constructing a metabolically modified microbial strain that produces useful compounds at high levels, comprising: (A) a step of constructing a base strain by metabolically modifying a parent strain; (B) a step of preparing unit DNA cassettes in which a promoter for high expression and a terminator sequence are linked to the DNA encoding each enzyme of a group of enzymes that constitute the biosynthesis pathway of the useful compound functionally within the cell, and unit DNA cassettes in which a promoter for low expression (or without a promoter) and a terminator sequence are linked to the DNA encoding each enzyme, and then performing gene assembly using the OGAB method to construct a plasmid library; (C) the above The method for constructing metabolically modified microbial strains includes the steps of: introducing the constructed plasmid library into the base strain and measuring the production amount of the useful compound of each strain; analyzing the sequence information of the plasmids introduced into each strain obtained in steps (D) and (C); performing statistical analysis or machine learning that correlates the production amount of the useful compound of each strain obtained in steps (E) and (C) with the sequence information of the plasmids obtained in step (D) to identify useful genes that contribute to the production of the useful compound; and creating a strain in which the useful genes that contribute to the production of the useful compound identified in steps (F) and (E) have been recombinant.

Building DNA strands at a high rate that are suitable for data storage. Methods include using DNAzyme and utilizing libraries of pre-prepared oligos. A system for the DNA strand synthesis includes: a DNA symbol library comprising a number of single strand oligo symbols; a DNA linker library comprising a first set of single strand oligo linkers and a second set of single strand oligo linkers; and a DNAzyme library comprising a number of DNAzymes. An S1 end of a first DNAzyme is adapted to join the S1 end of a symbol and an S2 end of the first DNAzyme is adapted to join an S2 end of a first linker, and an S1 end of a second DNAzyme is adapted to join an S1 end of a second linker and an S2 end of the second DNAzyme is adapted to join an S2 end of the symbol.