30 results about "Gene assembly" patented technology

The present invention is directed to methods and compositions for use of homologous recombination for directed evolution, gene reassembly, and directed mutagenesis. One aspect of the present invention relates to methods for use of bacterial conjugative transfer and homologous recombination for directed evolution, gene reassembly, and directed mutagenesis. Another aspect of the present invention relates to compositions for use in or produced by the methods of the present invention, including libraries, archives and databases.

A viral vector production system is provided which system comprises: (i) a viral genome comprising at least one first nucleotide sequence encoding a gene product capable of binding to and effecting the cleavage, directly or indirectly, of a second nucleotide sequence, or transcription product thereof, encoding a viral polypeptide required for the assembly of viral particles; (ii) a third nucleotide sequence encoding said viral polypeptide required for the assembly of the viral genome into viral particles, which third nucleotide sequence has a different nucleotide sequence to the second nucleotide sequence such that said third nucleotide sequence, or transcription product thereof, is resistant to cleavage directed by said gene product. The viral vector production system may be used to produce viral particles for use in treating or preventing viral infection.

The present invention is directed to methods and materials for RNA-mediated gene assembly from oligonucleotide sequences. In some embodiments, the oligonucleotides used for gene assembly are provided in an array format. An RNA polymerase promoter is appended to surface-bound oligonucleotides and a plurality of RNA copies of each oligonucleotide are then produced with an RNA polymerase. These RNA molecules self-assemble into a desired full-length RNA transcript by hybridization and ligation. The resulting RNA transcript may then be converted into double strand DNA useful in a variety of applications including protein expression.

The invention provides an informatics-based gene transcript assembly and quantification method and system, wherein the method comprises: aligning a sequencing read with a reference genome, and predicting initial start and end positions of a gene and transcript according to the alignment results for the sequencing read and reference genome; after prediction, establishing a directed graph to simulate possible transcripts to obtain a candidate transcript set; subjecting the candidate transcript set to transcript prediction and kurtosis estimation according the mode of maxium information transmission capacity. The method and system of the invention have the advantages that dependence on external gene positional marking is avoided, gene assembly accuracy is significantly improved, and sequencing precision is improved.

Embodiments relate to methods and compositions useful for routing and tracking multiple mobile units within a microfluidic device. Mobile units may be routed through a plurality of chemical environments, and the mobile units may be tracked to determine the path and / or environments that the mobile units have routed through. Mobile units may be routed in accordance with a predetermined algorithm. Mobile units may be routed through microfluidic devices in ordered flow. Mobile units routed through the microfluidic device can be used to perform various chemical reactions uniquely associated to the units, including without limitation peptide synthesis, enzymatic gene synthesis and gene assembly.

The invention discloses a recombinant CL7-CVN protein, its preparation method and application. The preparation method of the recombinant CL7-CVN protein comprises: cloning its coding sequence into an expression vector to obtain a recombinant expression vector; transforming the recombinant expression vector into a host cell for expression and purification to obtain a recombinant CL7-CVN protein. The present invention constructs a fusion expression vector through a synthetic CVN gene and a gene assembly method to realize its highly soluble expression in Escherichia coli; according to the heat resistance of the recombinant CL7-CVN protein, through heat treatment and 3C protease digestion, it can be passed One-step affinity chromatography quickly obtains recombinant CL7‑CVN protein and CVN protein with a purity of up to 99%. The preparation method provided by the invention can significantly increase the expression level of the recombinant protein, make the activity more stable, and the purification method is simpler; both the recombinant CL7‑CVN protein and the CVN protein prepared by the invention have antiviral activity.

The present invention provides an error correcting method of test sequence, which involves receiving test sequences, configuring high frequency short string list based on a preset high frequency threshold value, traversing each received test sequence, searching an area with the largest number of continuous high frequency short strings on each test sequence in combination with high frequency short string list, configuring whole left sequence and / or right sequence of high frequency short strings at left side and / or right side of searched area according to corresponding received test sequence and high frequency short string list, and constituting corresponding test sequence according to configured left and / or right sequence and searched area. The present invention also provides corresponding error correcting system of test sequence and gene assembly equipment.

The invention provides a gene engineering method for preparing a recombinant glutathion peroxidase, which belongs to the field of biological technology. The method comprises the following steps: assembling a target gene to a secretion type prokaryotic expression vector, introducing a catalytic group SeCys of GPX into a substrate combination position of GPX by a gene mutant method in an auxotroph prokaryotic expression system or an auxotroph and SPP combined expression system, thereby the activity of the GPS is very high; or assembling the target gene with the SeCys insertion sequence into a secretion type mammalia cell expression vector, assembling a SeCys insertion sequence binding protein 2 into an intracellular type mammalia cell expression vector, and cotransfecting the two vectors into the same lactation cell strain, and synthesizing the GPX under the existence of sodium selenite. The invention the advantages of simple method, recombined GPX vitality, high output and good stability, thereby avoiding decreased yield and inactivation caused by renaturation of an inclusion body, and solving the problem that the natural GPX source is limited and the property is not stable.