4 results about "Resistant virus" patented technology

The invention discloses a factory-like efficient breeding method and system for virus-free seed potatoes in a potato bottle, and relates to the technical field of agricultural biology. The method comprises the following steps: selecting healthy buds, stripping stem tips, carrying out primary culture to obtain seedlings, screening virus-free seedlings through RT-PCR (Reverse Transcription-Polymerase Chain Reaction) detection, and transferring the virus-free seedlings to a culture flask containing a liquid MS culture medium for expanding propagation; hardening the propagated virus-free seedlings to obtain stress-resistant virus-free seedlings, inoculating the stress-resistant virus-free seedlings into a tissue culture bottle containing an induction culture medium, performing induction culture under set illumination and temperature, and dynamically adjusting a nutrient solution to obtain virus-free seed potatoes; and classifying and screening the detoxified seed potatoes and storing in a specific environment. The system comprises a sterile operation unit, a multi-stage culture unit, a seedling hardening unit and a graded storage unit. The method can efficiently breed the virus-free potato seeds, reduces the virus infection risk, improves the breeding efficiency and the potato seed quality, and is suitable for industrialized production.

The application of OsFabF gene in resistance to rice stripe virus, the application relates to the technical field of rice disease resistance breeding. The application uses a CRISPR / Cas9 gene editing system to design sgRNA targeting a specific functional region of an endogenous OsFabF gene in rice, to precisely edit the region, thereby obtaining a mutant with a functionally deficient OsFabF. The editing preferably results in a frame shift mutation or a premature stop codon, to obtain a truncated OsFabF protein mutant. Experiments show that the rice mutant plant obtained in this way exhibits significantly enhanced resistance to RSV infection, reduced virus accumulation and reduced disease symptoms. The application provides an effective gene target and breeding material for cultivating new rice varieties resistant to rice stripe virus.

Disclosed in the present invention are pyridone derivatives, having structures represented by general formula I, general formula II or general formula III. Also disclosed in the present invention is a preparation method for the described derivatives, etc. Studies have shown that the resistance indexes (RI=IC50[I38T] / IC50[WT]) of the hydroxypyridone derivatives described in the present invention against wild-type and PA / I38T mutation-type influenza virus RNA polymerases are lower than that of baloxavir, which indicates that the derivatives of the present invention are less affected by PA / I38T mutation-type influenza A viruses and are superior to baloxavir. These derivatives can also effectively inhibit virus replication of avian influenza H5N1, and have lower cytotoxicity than baloxavir and have higher selectivity, and therefore can be used for preparing anti-influenza drugs for inhibiting baloxavir-resistant virus strains and avian influenza.