12 results about "Resistant virus" patented technology

The invention discloses a reagent and a method for detecting berberine-resistant viruses. The reagent provided by the invention comprises an antibody or an antigen binding fragment thereof, the antibody or the antigen binding fragment thereof comprises a heavy chain complementarity determining region HCDR1-3 and / or a light chain complementarity determining region LCDR1-3, the heavy chain complementarity determining region HCDR1-3 has a sequence as shown in SEQ ID NO.5-7, and the light chain complementarity determining region LCDR1-3 has a sequence as shown in SEQ ID NO.8-10. The reagent can be used for detecting the berberine-resistant herpes simplex virus type 1, so that the treatment effect can be evaluated in advance before berberine is applied for treatment.

The invention discloses a factory-like efficient breeding method and system for virus-free seed potatoes in a potato bottle, and relates to the technical field of agricultural biology. The method comprises the following steps: selecting healthy buds, stripping stem tips, carrying out primary culture to obtain seedlings, screening virus-free seedlings through RT-PCR (Reverse Transcription-Polymerase Chain Reaction) detection, and transferring the virus-free seedlings to a culture flask containing a liquid MS culture medium for expanding propagation; hardening the propagated virus-free seedlings to obtain stress-resistant virus-free seedlings, inoculating the stress-resistant virus-free seedlings into a tissue culture bottle containing an induction culture medium, performing induction culture under set illumination and temperature, and dynamically adjusting a nutrient solution to obtain virus-free seed potatoes; and classifying and screening the detoxified seed potatoes and storing in a specific environment. The system comprises a sterile operation unit, a multi-stage culture unit, a seedling hardening unit and a graded storage unit. The method can efficiently breed the virus-free potato seeds, reduces the virus infection risk, improves the breeding efficiency and the potato seed quality, and is suitable for industrialized production.

The invention discloses a kit for detecting berberine-resistant viruses and application of the kit. The kit provided by the invention comprises an antibody or an antigen binding fragment thereof, a buffer agent and a signal generation system, the antibody or the antigen binding fragment thereof comprises a heavy chain complementarity determining region HCDR1-3 and a light chain complementarity determining region LCDR1-3, the sequences of the heavy chain complementarity determining region HCDR1-3 are respectively as shown in SEQ ID NO.5-7, the sequences of the light chain complementarity determining region LCDR1-3 are respectively as shown in SEQ ID NO.8-10, and the signal generation system comprises a buffer agent and a signal generation system. Or the sequences of the heavy chain complementarity determining region HCDR1-3 are respectively shown as SEQ ID NO.15-17, and the sequences of the light chain complementarity determining region LCDR1-3 are respectively shown as SEQ ID NO.18-20. The kit can be used for detecting the berberine-resistant herpes simplex virus type 1, so that the treatment effect can be evaluated in advance before berberine is applied for treatment.

The application of OsFabF gene in resistance to rice stripe virus, the application relates to the technical field of rice disease resistance breeding. The application uses a CRISPR / Cas9 gene editing system to design sgRNA targeting a specific functional region of an endogenous OsFabF gene in rice, to precisely edit the region, thereby obtaining a mutant with a functionally deficient OsFabF. The editing preferably results in a frame shift mutation or a premature stop codon, to obtain a truncated OsFabF protein mutant. Experiments show that the rice mutant plant obtained in this way exhibits significantly enhanced resistance to RSV infection, reduced virus accumulation and reduced disease symptoms. The application provides an effective gene target and breeding material for cultivating new rice varieties resistant to rice stripe virus.

[Problem] To provide an antiviral oligonucleotide that acts on cells infected by viruses having multiple RNA genomes, exhibiting an antiviral effect, and does not tend to the emergence of resistant viruses, and a pharmacological composition thereof. [Solution] An oligonucleotide that takes on a G-quadruplex structure that inhibits the binding of the genomic RNA and nucleocapsid (N) protein of a virus having an RNA genome, the antiviral oligonucleotide suppressing proliferation of the virus by contact with virus-infected cells, and a pharmacological composition thereof are used.

The invention provides application of a targeted Hsp90 alpha compound in treatment of infectious encephalitis and improvement of clinical drug resistance, and belongs to the technical field of medicines. The targeted Hsp90 alpha compound disclosed by the invention has a structure as shown in a formula 1, and a parent nucleus structure of the targeted Hsp90 alpha compound is (3, 6, 6-trimethyl-4-oxo-4, 5, 6, 7-tetrahydro-1H-indazole-1-yl) benzamide. The targeted Hsp90 alpha compound can effectively treat infectious encephalitis, can treat infectious encephalitis caused by acyclovir and other nucleoside drug resistance viruses, and improves the clinical drug resistance problem of infectious encephalitis.

Biopolymer compositions comprising antimicrobial peptides (AMPs) for treating infections such as bacterial infections, viral infections, fungal infections, and parasitic infections. The compositions herein may also be used for treating infections associated with antibiotic-resistant bacteria, antifungal-resistant fungi, antiviral-resistant viruses, or for treating biological warfare agents (BWAs) such as Bacillus anthracis and Yersenia pestis. The present invention also provides methods of synthesis of said biopolymer compositions, wherein AMP biopolymers can be synthesized as an artificially engineered protein by genetically fusing an AMP; a protein that behaves similarly to polymer tethers; and a protein as a modifiable material platform that can transform to self-assembled nanoparticles, self-standing films, or adhesives to easily attach tethered AMPs onto any biomaterial surface for various clinical applications.

Disclosed in the present invention are pyridone derivatives, having structures represented by general formula I, general formula II or general formula III. Also disclosed in the present invention is a preparation method for the described derivatives, etc. Studies have shown that the resistance indexes (RI=IC50[I38T] / IC50[WT]) of the hydroxypyridone derivatives described in the present invention against wild-type and PA / I38T mutation-type influenza virus RNA polymerases are lower than that of baloxavir, which indicates that the derivatives of the present invention are less affected by PA / I38T mutation-type influenza A viruses and are superior to baloxavir. These derivatives can also effectively inhibit virus replication of avian influenza H5N1, and have lower cytotoxicity than baloxavir and have higher selectivity, and therefore can be used for preparing anti-influenza drugs for inhibiting baloxavir-resistant virus strains and avian influenza.

The application discloses a neuraminidase inhibitor based on a hydrophobic tag technology and a preparation method, a derivative, a pharmaceutical composition and an application thereof, and the structure of the neuraminidase inhibitor is shown as formula (I). The neuraminidase inhibitor based on the hydrophobic tag technology has enhanced antiviral activity and effective oseltamivir resistance reversal activity, and provides a new drug for the treatment of influenza virus infection, especially oseltamivir-resistant virus infection.

The invention provides application of venenum bufonis and bufalin in preparation of a herpes simplex virus inhibitor, herpes simplex virus is HSV-1 or acyclovir drug-resistant virus strain HSV-1 / 153, a disease caused by HSV infection is herpes keratitis or herpes progenitalis disease infection, and the invention further discloses a pharmaceutical composition. Venenum bufonis and bufalin are used as active components or main active components, and a pharmaceutically acceptable carrier is supplemented.

The application discloses a reagent and a method for detecting berberine-resistant viruses. The reagent provided by the application comprises an antibody or an antigen binding fragment thereof, and the antibody or the antigen binding fragment thereof comprises heavy chain complementarity determining regions HCDR1-3 and / or light chain complementarity determining regions LCDR1-3, wherein the heavy chain complementarity determining regions HCDR1-3 have sequences shown in SEQ ID NO. 5-7 respectively, and the light chain complementarity determining regions LCDR1-3 have sequences shown in SEQ ID NO. 8-10 respectively. The reagent of the application can be used for detecting berberine-resistant herpes simplex virus 1, so that the treatment effect can be pre-evaluated before berberine treatment.