7 results about "Gene Position" patented technology

The invention discloses an image processing method and system based on an in-situ hybridization technology and a medium, and relates to the technical field of biological information, and the method comprises the following steps: converting a cell DAPI dyeing result into two-dimensional data from three-dimensional data by utilizing Z-axis maximum intensity projection, identifying and separating a single cell from the two-dimensional data by utilizing cell segmentation, and when the cell segmentation is used for processing an overlapping region, identifying and separating the single cell from the two-dimensional data. Using a registration algorithm Ashlar to calculate an error between adjacent visual fields of the same round to obtain cell position information; an imaging result after hybridization of the fluorescent probe and the gene is subjected to multiple rounds of registration through a registration algorithm Ashlar to generate a spliced image, local maximum values of the spliced image under different rounds are marked by using a fluorescent dot recognition algorithm, so that position information of each fluorescent dot is obtained, the position information of each fluorescent dot corresponds to the gene in a transcript, and the position information of each fluorescent dot corresponds to the gene in the transcript. Obtaining gene position information; distributing genes into cells by utilizing the cell position information and the gene position information to obtain a cell gene matrix and displaying the cell gene matrix; according to the image processing method and system and the medium, accurate matching of sequencing data and spatial information is achieved, and then the distribution rule of gene expression in space is better revealed.

The present application is directed to a method for identifying at least one candidate (Loc), namely a gene location and / or a sequence variant, indicative for at least one selected (phenotypic) trait of an organism, in particular of a plant, comprising the steps of:a. receiving a plurality of candidate lists (Can1, Can2, Can3) of candidates (Loc), the candidate lists being ordered;b. receiving a reference set (RefDB) with gene locations and / or sequence variants;c. matching at least a subset of candidates (Loc) from the candidate lists (Can1, Can2, Can3) with the reference list (RefDB) to determine an evaluation value (EV) for at least the subset;d. assigning each evaluation value (EV) to the respective candidate (Loc) in the respective candidate lists (Can1, Can2, Can3);e. calculating for each candidate list a performance value based on the evaluation value (EV), in particular by using the evaluation values (EV);f. selecting at least one candidate (Loc) as (preferred) candidate (Loc) from one of the candidate lists (Can1, Can2, Can3) using the performance values.

The present invention belongs to the field of genetic breeding technology, and specifically relates to a haplotype molecular marker affecting the thickness of sheep loin muscle and its application. The haplotype molecular marker includes CELF4 ‑SNP1~ CELF4 ‑SNP2 has 2 sites; among them, CELF4 -SNP1 is located at the 101bp position of the nucleotide sequence shown in SEQ ID NO.1, where a G to C mutation occurs; CELF4 The gene position of SNP2 is 101 bp of the nucleotide sequence shown in SEQ ID NO. 2, where a mutation from G to C occurs. The haplotype molecular marker of the present invention can be used to identify the psoas muscle thickness trait in sheep. By selecting individuals with the haplotype combination of GGGG as the sire or dam, the psoas muscle thickness of the offspring can be increased.

The application discloses a multi-gene discovery network construction method and device, equipment and a storage medium. The method comprises the following steps: acquiring genome information input by a user, constructing a discovery network among all to-be-encoded genes according to the genome information, and determining storage information of each to-be-encoded gene; generating a DNA sequence according to the discovery network and the storage information, sequencing the DNA sequence, and obtaining a sequencing result; decoding the sequencing result, obtaining gene position information, judging whether there is an encoded gene according to the gene position information, quickly and efficiently finding the corresponding gene during decoding, saving the gene searching time, quickly detecting the gene report error, avoiding the sequencing result error caused by multiple variations and the sequencing itself, and improving the speed and efficiency of the multi-gene discovery network construction.

The invention discloses a malignant cell separation method and system based on transcriptome sequencing. The method comprises the following steps: step 1, reading and preprocessing transcriptome sequencing data; step 2, calculating a window CNV based on a gene position; 3, respectively calculating a patient-level CNV threshold value and a sample-level CNV threshold value; step 4, performing reference correlation analysis, including reference cell determination, cell correlation calculation and correlation threshold determination; 5, performing cell classification by integrating the CNV threshold value and the correlation; step 6, verifying independence; and step 7, generating an analysis result and a visual chart. The problem of tumor heterogeneity can be more accurately solved, more accurate separation of malignant cells is realized, and the method particularly has obvious advantages in a complex tumor environment in which a traditional fixed threshold method fails.

The present application is directed to a method for identifying at least one candidate (Loc), namely a gene location and / or a sequence variant, indicative for at least one selected (phenotypic) trait of an organism, in particular, of a plant.

The application discloses a corn southern rust resistance gene function marker, the gene is located on the 6th chromosome of corn, from the main QTL site RppQ6.01 of the southern rust resistance of Zi 319, gene silencing test shows that ZmWRKY66 is the disease resistance gene of RppQ6.01, sequence analysis finds that there is an insertion / deletion of CACTA-TIR transposon in the promoter region of ZmWRKY66, two function markers are developed based on the DNA sequence of the transposon, and are named as CACTA-L and CACTA-R. In the application, the main QTL and gene position of the corn southern rust resistance are clear, the phenotype contribution rate is higher, the southern rust resistance of corn inbred line material can be significantly improved, the development and application of the two function markers make the corn seedling stage ZmWRKY66 gene typing detection convenient and fast, and the application is not affected by the environment, and a feasible technical approach is provided for the molecular assisted breeding of the corn southern rust resistance.