Method for positioning and displaying biological gene expression information and environmental sensitive area on chromosomes

Abstract

The invention provides a method for positioning and displaying biological gene expression information and environmental sensitive area on chromosomes. The method comprises the following steps: downloading genomic sequence of biological chromosomes; reading in the gene expression microarray data of the corresponding biological sample and then performing contrast treatment, logarithm processing and normalization processing to obtain environmental condition value; matching gene names in the gene expression microarray data matrix with the downloaded gene names in gene sequence of the biological chromosomes and then positioning the environmental condition value of the gene on the corresponding gene position in the biological genomic sequence; obtaining gene expression images and environmental sensitive areas on the biological chromosome genome according to the positioned environmental condition value. According to the method, the visualization function of distribution characteristics of expressed variant genes on the chromosomes is achieved, the distribution characteristic data of the genes in the environmental sensitive areas is obtained, which provides effective help for analysis of expressed variant sensitive areas of genes on the chromosomes and speculation of environmental sensitive transcription factors.

Description

technical field [0001] The invention belongs to the technical field of biological information, and relates to a method capable of locating gene expression information on chromosomes and then analyzing sensitive regions of gene expression changes on the chromosomes. Background technique [0002] Gene chip technology uses methods such as photoconductive in-situ synthesis or direct micro-spotting to orderly solidify a large number of DNA fragments or oligonucleotide fragments on the surface of filter membranes, silicon wafers and other supports, and use isotopes or fluorescent molecules to detect samples. After labeling, it is hybridized with the microarray, and the number and sequence information of the sample molecules are obtained by detecting the hybridization signal intensity of each probe molecule. At present, gene chips are mainly used in the detection of gene expression levels, gene diagnosis, drug screening, personalized medicine, sequencing services and bioinformatics...

AI Technical Summary

Problems solved by technology

Although the original C. elegans whole-genome expression data measured by the gene chip has complete gene expression information of C. elegans, it is not sorted according to the corresponding positions of C. elegans genes on the chromosome.

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