Method for positioning and displaying biological gene expression information and environmental sensitive area on chromosomes

A technology of chromosome gene and gene expression, which is applied in the field of bioinformatics and can solve problems such as ordering

Inactive Publication Date: 2014-12-03
DALIAN MARITIME UNIVERSITY
View PDF5 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the original C. elegans whole-genome expression data measured by the gene chip has complete gene expression inf

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for positioning and displaying biological gene expression information and environmental sensitive area on chromosomes
  • Method for positioning and displaying biological gene expression information and environmental sensitive area on chromosomes
  • Method for positioning and displaying biological gene expression information and environmental sensitive area on chromosomes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0039] The construction and results of the present invention will be described in detail below mainly in conjunction with the accompanying drawings and specific embodiments. However, those skilled in the art will understand that the following examples are only used to illustrate the present invention, and should not be considered as limiting the scope of the present invention.

[0040] The present invention will be described in detail below by taking the whole genome of nematodes as an example.

[0041] Nematode Whole Genome Gene Expression Microarray: Shanghai Kangcheng Biological Co., Ltd.

[0042] The genome-wide gene expression microarray data of nematodes, including two sets of data, are:

[0043] The first group is the test group: after 16.5 days of treatment under the conditions of the space flight environment, the nematodes were collected, and total RNA was extracted, and ds-cDNA was synthesized using the Invitrogen Superscript kit, and single-color fluorescently labe...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a method for positioning and displaying biological gene expression information and environmental sensitive area on chromosomes. The method comprises the following steps: downloading genomic sequence of biological chromosomes; reading in the gene expression microarray data of the corresponding biological sample and then performing contrast treatment, logarithm processing and normalization processing to obtain environmental condition value; matching gene names in the gene expression microarray data matrix with the downloaded gene names in gene sequence of the biological chromosomes and then positioning the environmental condition value of the gene on the corresponding gene position in the biological genomic sequence; obtaining gene expression images and environmental sensitive areas on the biological chromosome genome according to the positioned environmental condition value. According to the method, the visualization function of distribution characteristics of expressed variant genes on the chromosomes is achieved, the distribution characteristic data of the genes in the environmental sensitive areas is obtained, which provides effective help for analysis of expressed variant sensitive areas of genes on the chromosomes and speculation of environmental sensitive transcription factors.

Description

technical field [0001] The invention belongs to the technical field of biological information, and relates to a method capable of locating gene expression information on chromosomes and then analyzing sensitive regions of gene expression changes on the chromosomes. Background technique [0002] Gene chip technology uses methods such as photoconductive in-situ synthesis or direct micro-spotting to orderly solidify a large number of DNA fragments or oligonucleotide fragments on the surface of filter membranes, silicon wafers and other supports, and use isotopes or fluorescent molecules to detect samples. After labeling, it is hybridized with the microarray, and the number and sequence information of the sample molecules are obtained by detecting the hybridization signal intensity of each probe molecule. At present, gene chips are mainly used in the detection of gene expression levels, gene diagnosis, drug screening, personalized medicine, sequencing services and bioinformatics...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G06F19/20
Inventor 马宝山杨存敏高英徐丹董辉孙野青
Owner DALIAN MARITIME UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap