Molecular marker affecting eriscerated yield of chicken and application of molecular marker
A technology of molecular markers and genotyping, which is applied in the direction of recombinant DNA technology, microbial measurement/inspection, DNA/RNA fragments, etc., can solve the problems of long breeding cycle, low selection intensity, and high breeding cost, so as to reduce the breeding cycle, The effect of increasing selection intensity and reducing breeding cost
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experiment example 2
[0041] Experimental Example 2 Obtaining Molecular Markers Affecting Whole Eviscerated Weight of Chickens
[0042] (1) Blood sample collection
[0043] Vacuum blood collection tubes containing EDTA-dipotassium were used to collect blood from chicken wing veins.
[0044] (2) Extraction and identification of genomic DNA
[0045] GentraPuregene Blood Kit (Qiagen) column centrifugation kit was used to extract blood genomic DNA. After the DNA was completely dissolved, its concentration was detected with a NannoDrop nucleic acid analyzer, and the integrity of the DNA band was detected under 1% agarose gel electrophoresis conditions. .
[0046] (3) DNA library construction and sequencing
[0047] The construction of the library was completed using NEBNext DNA librarykit (NewEngland Biolabs). The specific operation steps were as follows: firstly, the genomic DNA was randomly broken into small fragments, and then Illumina double-end adapters were connected to the fragments with a lengt...
experiment example 3
[0064] Experimental example 3 A kind of molecular marker genotyping detection method that affects chicken whole eviscerated weight
[0065] (1) Experimental animals
[0066] The experimental flock group used in the present invention is 200 (100 males and females) of 150-day-old Daweishan miniature breeder chickens of Yunnan Agricultural University, which is the core group of breeder chickens. Slaughter experiments were carried out to determine the eviscerated weight.
[0067] (2) Target DNA sequence amplification and sequencing
[0068] 1) Primer design
[0069] The DNA sequence of the chondrolectin (CHODL) gene on chicken chromosome 1 was downloaded from the NCBI website, and primers were designed using the primer design software primer premier 6.0.
[0070] The DNA sequences of the designed primers are as follows:
[0071] Upstream primer primer-F: 5'-CGCAGATACCAGGGAGATCA-3';
[0072] Downstream primer primer-R: 5'-CAGAAGCAGGGAGGTGATTG-3'.
[0073] 2) PCR amplification...
experiment example 4
[0083] Experimental Example 4 Molecular marker SNP site g.328T>C effect analysis
[0084] The invention provides a SNP molecular marker capable of significantly improving the growth and development of chickens, and using the SNP molecular marker for marker-assisted selection can greatly accelerate the growth and development breeding process of chickens. If the present invention selects and breeds all TC-type individuals of molecular markers that affect chicken meat production performance into CC-type individuals, the average whole eviscerated weight of the Daweishan miniature chicken breeder population can be increased by 84.72g, which can significantly improve the meat production of breeder chickens performance.
[0085] Among individuals with this SNP molecular marker, by optimizing the dominant allele (C) of the SNP in Yunnan local chickens, the economic benefits of commercial chickens can be improved, thereby increasing the profits of enterprises.
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