Molecular marker affecting eriscerated yield of chicken and application of molecular marker

A technology of molecular markers and genotyping, which is applied in the direction of recombinant DNA technology, microbial measurement/inspection, DNA/RNA fragments, etc., can solve the problems of long breeding cycle, low selection intensity, and high breeding cost, so as to reduce the breeding cycle, The effect of increasing selection intensity and reducing breeding cost

Active Publication Date: 2020-11-20
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, half of China's domestic chicken market is still white-feathered broiler chickens that are highly selected and bred abroad, which are easily affected by the price of foreign provenance, and the variety is diversified. However, the local seed industry has not really established an industry with local breeds as the core technology. The development model has led to a serious lag in the development and utilization of local resources. The work of using domestic chicken resources to breed new varieties is not in place, the breeding methods are backward, and there are few quantitative measurement equipment and facilities for some highly automated and accurate traits.

Method used

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  • Molecular marker affecting eriscerated yield of chicken and application of molecular marker
  • Molecular marker affecting eriscerated yield of chicken and application of molecular marker
  • Molecular marker affecting eriscerated yield of chicken and application of molecular marker

Examples

Experimental program
Comparison scheme
Effect test

experiment example 2

[0041] Experimental Example 2 Obtaining Molecular Markers Affecting Whole Eviscerated Weight of Chickens

[0042] (1) Blood sample collection

[0043] Vacuum blood collection tubes containing EDTA-dipotassium were used to collect blood from chicken wing veins.

[0044] (2) Extraction and identification of genomic DNA

[0045] GentraPuregene Blood Kit (Qiagen) column centrifugation kit was used to extract blood genomic DNA. After the DNA was completely dissolved, its concentration was detected with a NannoDrop nucleic acid analyzer, and the integrity of the DNA band was detected under 1% agarose gel electrophoresis conditions. .

[0046] (3) DNA library construction and sequencing

[0047] The construction of the library was completed using NEBNext DNA librarykit (NewEngland Biolabs). The specific operation steps were as follows: firstly, the genomic DNA was randomly broken into small fragments, and then Illumina double-end adapters were connected to the fragments with a lengt...

experiment example 3

[0064] Experimental example 3 A kind of molecular marker genotyping detection method that affects chicken whole eviscerated weight

[0065] (1) Experimental animals

[0066] The experimental flock group used in the present invention is 200 (100 males and females) of 150-day-old Daweishan miniature breeder chickens of Yunnan Agricultural University, which is the core group of breeder chickens. Slaughter experiments were carried out to determine the eviscerated weight.

[0067] (2) Target DNA sequence amplification and sequencing

[0068] 1) Primer design

[0069] The DNA sequence of the chondrolectin (CHODL) gene on chicken chromosome 1 was downloaded from the NCBI website, and primers were designed using the primer design software primer premier 6.0.

[0070] The DNA sequences of the designed primers are as follows:

[0071] Upstream primer primer-F: 5'-CGCAGATACCAGGGAGATCA-3';

[0072] Downstream primer primer-R: 5'-CAGAAGCAGGGAGGTGATTG-3'.

[0073] 2) PCR amplification...

experiment example 4

[0083] Experimental Example 4 Molecular marker SNP site g.328T>C effect analysis

[0084] The invention provides a SNP molecular marker capable of significantly improving the growth and development of chickens, and using the SNP molecular marker for marker-assisted selection can greatly accelerate the growth and development breeding process of chickens. If the present invention selects and breeds all TC-type individuals of molecular markers that affect chicken meat production performance into CC-type individuals, the average whole eviscerated weight of the Daweishan miniature chicken breeder population can be increased by 84.72g, which can significantly improve the meat production of breeder chickens performance.

[0085] Among individuals with this SNP molecular marker, by optimizing the dominant allele (C) of the SNP in Yunnan local chickens, the economic benefits of commercial chickens can be improved, thereby increasing the profits of enterprises.

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Abstract

The invention discloses a molecular marker affecting the eriscerated yield of chicken and application of the molecular marker, and belongs to the technical fields of molecular marker-assisted selection and animal genetics and breeding. The molecular marker affecting the eriscerated yield of chicken is obtained by genome-wide association (GWAS) analysis on 741 test chickens from F2 generation resource population formed by Daweishan miniature chickens and recessive white plymouth rock, nucleotide single base mutation T>C (named: Chr.1 100911059T>C) exists at 100911059bp on chromosome 1 of the chicken ginseng genome Gallus_gallus. GRCg6a version, a gene positioned nearby the nucleotide single base mutation is gene of CHODL (chondrolectin), and the chicken eriscerated yield is significantly affected by mutation. The invention discloses acquisition and application of the molecular marker. The invention also provides a genotyping detection method for the molecular marker affecting the chicken eriscerated yield. An efficient and accurate molecular marker-assisted breeding technology can be established by the method, and is applied to genetic improvement of chicken meat production performance, so that chicken meat production is increased.

Description

technical field [0001] The invention relates to the field of molecular marker-assisted selection technology and animal genetic breeding technology, in particular to a molecular marker affecting chicken eviscerated weight and its application. Background technique [0002] As the global population continues to grow, chicken and eggs are the two types of animal protein that can be purchased with the highest grams of animal protein per currency except milk, and they are relatively competitive in terms of production costs. In China, chicken is the second largest meat product, accounting for 15% of meat production, and its ratio is increasing year by year. In 2019, the output of pig, beef, mutton and poultry in my country was 76.49 million tons, of which, the output of beef was 6.67 million tons, an increase of 3.6%; the output of mutton was 4.88 million tons, an increase of 2.6%; the output of poultry was 22.39 million tons, an increase of 12.3%; 33.09 million tons, an increase o...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCC12Q1/6888C12Q1/6858C12Q2600/124C12Q2600/156C12Q2600/172C12Q2531/113C12Q2535/122Y02P60/87
Inventor 贾俊静黄英王坤何洋豆腾飞简宗辉孙帅闫世雄荣华曹玮娜徐志强谷大海葛长荣
Owner YUNNAN AGRICULTURAL UNIVERSITY
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