SNP (Single Nucleotide Polymorphism) molecular marker related to rabbit muscle quality character and application of SNP molecular marker
A molecular marker, muscle quality technology, applied in the field of molecular biotechnology and molecular markers, to achieve the effect of saving cost, good accuracy and improving selection strength
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[0044] (1) Use the Invitrogen whole blood DNA extraction kit to extract the genomic DNA from the blood samples of the meat rabbits to be tested, and then use the NanoDrop2000 instrument for OD value detection, 1.25% agarose gel electrophoresis detection, and after the DNA quality inspection is qualified, transfer To a 96-well plate, store at -20°C for later use.
[0045] (2) take the genomic DNA of the meat rabbit to be tested as a template, carry out PCR reaction with the PCR amplification primers (SEQ ID NO: 2 and SEQ ID NO: 3) in the primer set to obtain a PCR product, after the reaction finishes Store at 4°C;
[0046] The reaction volume of the PCR reaction was 5 μL, including 1 μL of 20ng / μL genomic DNA, 0.625 μL of 10× PCR reaction buffer, and 25 mmol / L MgCl 2 0.325μL, 25μmol / L dNTPs 0.1μL, 0.5μmol / L upstream primer (SEQ ID NO: 2) and downstream primer (SEQ ID NO: 3) total 1 μL, 5U / μL Taq DNA polymerase 0.1 μL, deionized water 1.85 μL;
[0047] The reaction program of...
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