SNP (Single Nucleotide Polymorphism) molecular marker related to rabbit muscle quality character and application of SNP molecular marker

A molecular marker, muscle quality technology, applied in the field of molecular biotechnology and molecular markers, to achieve the effect of saving cost, good accuracy and improving selection strength

Pending Publication Date: 2022-07-15
SICHUAN ANIMAL SCI ACAD
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  • Description
  • Claims
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Problems solved by technology

However, so far there are no related studies on the rabbit IGF2R gene

Method used

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  • SNP (Single Nucleotide Polymorphism) molecular marker related to rabbit muscle quality character and application of SNP molecular marker
  • SNP (Single Nucleotide Polymorphism) molecular marker related to rabbit muscle quality character and application of SNP molecular marker
  • SNP (Single Nucleotide Polymorphism) molecular marker related to rabbit muscle quality character and application of SNP molecular marker

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Experimental program
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Embodiment 1

[0044] (1) Use the Invitrogen whole blood DNA extraction kit to extract the genomic DNA from the blood samples of the meat rabbits to be tested, and then use the NanoDrop2000 instrument for OD value detection, 1.25% agarose gel electrophoresis detection, and after the DNA quality inspection is qualified, transfer To a 96-well plate, store at -20°C for later use.

[0045] (2) take the genomic DNA of the meat rabbit to be tested as a template, carry out PCR reaction with the PCR amplification primers (SEQ ID NO: 2 and SEQ ID NO: 3) in the primer set to obtain a PCR product, after the reaction finishes Store at 4°C;

[0046] The reaction volume of the PCR reaction was 5 μL, including 1 μL of 20ng / μL genomic DNA, 0.625 μL of 10× PCR reaction buffer, and 25 mmol / L MgCl 2 0.325μL, 25μmol / L dNTPs 0.1μL, 0.5μmol / L upstream primer (SEQ ID NO: 2) and downstream primer (SEQ ID NO: 3) total 1 μL, 5U / μL Taq DNA polymerase 0.1 μL, deionized water 1.85 μL;

[0047] The reaction program of...

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Abstract

The invention belongs to the technical field of molecular biotechnology and molecular markers, and discloses an SNP molecular marker related to rabbit muscle quality traits and application of the SNP molecular marker, the nucleotide sequence of the SNP molecular marker is shown as SEQ ID No.1, the SNP site corresponds to the 149848214bp site of the 12th chromosome of the rabbit genome sequence OryCann2.0 version, and the site has G / A base mutation. The invention further provides a primer group for identifying the SNP molecular marker, the meat rabbit muscle quality character can be determined through the molecular marker and the primer group, and a reliable tool is provided for breeding of a new meat rabbit variety with excellent meat quality; by optimizing the dominant alleles of the SNP molecular marker, the breeding genetic progress of rabbits can be accelerated, the breeding cost is saved, and the breeding economic benefit is improved.

Description

technical field [0001] The invention belongs to the technical field of molecular biotechnology and molecular markers, and in particular relates to a SNP molecular marker related to rabbit muscle quality traits and its application. Background technique [0002] Rabbit meat, as a high-protein, low-fat and low-cholesterol food, is nutritious and does not make people fat. It is an ideal "beauty food" and is increasingly favored by people. my country is the world's largest rabbit-raising country, with both domestic rabbit breeding and rabbit product output ranking first in the world. However, for a long time, the improved breed of meat rabbits has relied on imports. Although the imported matching lines have a fast growth rate, due to excessive pursuit of growth rate, their On the contrary, the muscle quality has become worse and worse, which cannot meet people's demand for high-quality rabbit meat, which has seriously affected the sustainable development of my country's rabbit ind...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCC12Q1/6888C12Q1/6858C12Q2600/156C12Q2600/124C12Q2531/113C12Q2521/525Y02P60/87
Inventor 邝良德李丛艳李钰莹梅秀丽雷岷郭志强郑洁杨锐谢晓红季杨任永军张翔宇张翠霞杨超唐丽袁定胜
Owner SICHUAN ANIMAL SCI ACAD
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