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Breeding method of a semi-vine type high-yielding peanut variety

A variety of peanut technology, applied in the agricultural field, can solve problems such as unfavorable mechanized digging and weak branches, and achieve the effects of saving breeding workload, speeding up the breeding process, and shortening the generation interval

Active Publication Date: 2019-04-09
CROP RES INST GUANGDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the upright peanut plant type, the clusters are compact and the branches are weak, which is not conducive to mechanized digging

Method used

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  • Breeding method of a semi-vine type high-yielding peanut variety
  • Breeding method of a semi-vine type high-yielding peanut variety
  • Breeding method of a semi-vine type high-yielding peanut variety

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1. Directed screening of hybrid parents using three SSR molecular marker primer sets related to yield traits

[0031] 1. According to the survey results of peanut traits (Table 1), randomly select 9 local varieties of semi-climbing peanuts (Wuchuan Mengdou, Yongfa Douyi, Gaohe No. , Huilai Axe, Shengtengxi, Zhuyouzi), and 9 varieties of erect flowers (Yueyou No. 5, Zhongkaihua No. 1, Shanyou 851, Zhanyou 75, Heyou No. 9, Yueyou 52, Yueyou 13, Yueyou 79, Hanghua 2), provided by the Crop Research Institute of Guangdong Academy of Agricultural Sciences.

[0032] 2. The peanuts of the above 16 varieties are germinated, and when the sprouts grow about 1 cm, cut out the sprouts and extract DNA. According to the operating instructions of the Plant Genomic DNA Extraction Kit (spin column type) of Beijing Biotech Biotechnology Co., Ltd., use 1% agarose gel electrophoresis for detection, dilute the sample to 25ng μL-1, and store it at -20°C for later use .

[0033] 3. Using th...

Embodiment 2

[0049] 1. Among the semi-vine varieties, choose the variety Katsudosei with a statistical result of 0-0-0 as the hybrid female parent, and the traits of the female parent are semi-vine peanuts. Among the upright varieties, the variety Hanghua No. 2 with a statistical result of 1-1-1 was selected as the hybrid male parent, and the traits of the male parent are upright high-yield and disease-resistant varieties. F1 generation seeds were obtained by crossing.

[0050] 2. The spare seeds of the F1 generation are sown in a single seed, self-crossed, and harvested in a single plant. Get the F2 generation seeds

[0051] 3. Sow single seed of F2 generation spare seeds, self-cross, after the peanut leaves grow, take the young peanut leaves, extract DNA, amplify with markers GM1445, GM12531, GM2589, and select the statistical result as 1 according to the band pattern results For peanut plants of -1-1, a single plant of the semi-climb type is selected from the above-mentioned peanut pl...

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Abstract

The invention provides a breeding method of a semiprostrate high-yield peanut variety. The breeding method is a method of screening male parent and female parent peanut varieties for hybridization by means of three yield trait related SSR marked primer units: GM1445, GM2531 and GM2589. The breeding method provided by the invention is a novel variety breeding method capable of predicting the yield condition of single strains on the premise of no need to wait for harvesting and polymerizing semiprostrate and high-yield trait to a same peanut plant. The method has the advantages of being simple in breeding method, clear in breeding target, small in blindness, high in efficiency and etc., and the output result can be suitable for the general orientation of agricultural mechanization in the future.

Description

technical field [0001] The invention relates to the technical field of agriculture, in particular to a breeding method for a semi-vine type high-yield peanut variety. Background technique [0002] Peanut is one of the four major oil crops in the world, and it is also an important oil crop and economic crop in my country. For a long time, peanut breeding has been mainly based on traditional hybrid breeding, which has a long breeding cycle and a single genetic basis for the bred varieties. With the development of genome sequencing technology and molecular quantitative genetics, a peanut genetic map based on SSR markers has been constructed, and genes / QTLs related to disease resistance, stress resistance, yield, quality, etc. have been located, and molecular markers have also been proposed. Assisted selection breeding techniques. However, the target traits of peanut genetic improvement, especially the yield traits, are mostly quantitative traits, which are controlled by micro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12Q1/6858A01H1/02A01H1/04
CPCA01H1/02A01H1/04C12Q1/6895C12Q2600/13C12Q2600/156
Inventor 李杏瑜洪彦彬温世杰梁炫强陈小平
Owner CROP RES INST GUANGDONG ACAD OF AGRI SCI
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